PANG2_DROME
ID PANG2_DROME Reviewed; 1192 AA.
AC Q8IMA8; A0AVW4;
DT 26-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT 13-NOV-2007, sequence version 2.
DT 03-AUG-2022, entry version 158.
DE RecName: Full=Protein pangolin, isoform J;
DE AltName: Full=dTCF;
GN Name=pan {ECO:0000312|FlyBase:FBgn0085432};
GN Synonyms=TCF {ECO:0000303|PubMed:9118222}; ORFNames=CG34403;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1] {ECO:0000312|EMBL:AAN06549.2}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley {ECO:0000269|PubMed:10731132};
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2] {ECO:0000305, ECO:0000312|EMBL:AAN06549.2}
RP GENOME REANNOTATION, AND ALTERNATIVE SPLICING.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3] {ECO:0000305, ECO:0000312|EMBL:ABK30919.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 804-1192.
RC STRAIN=Berkeley;
RA Stapleton M., Carlson J.W., Frise E., Kapadia B., Park S., Wan K.H., Yu C.,
RA Celniker S.E.;
RL Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases.
RN [4] {ECO:0000305}
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 831-1192.
RC STRAIN=Crete24, Crete26, Crete30, Crete31, Crete35, Crete40, Crete42,
RC Crete43, Crete44, Crete8, FTF1, FTF105, FTF14, FTF2, FTF20, FTF23, FTF26,
RC FTF28, FTF5, FTF6, Zim11, Zim2, Zim30, and Zim53;
RX PubMed=14668375; DOI=10.1093/genetics/165.3.1195;
RA Sheldahl L.A., Weinreich D.M., Rand D.M.;
RT "Recombination, dominance and selection on amino acid polymorphism in the
RT Drosophila genome: contrasting patterns on the X and fourth chromosomes.";
RL Genetics 165:1195-1208(2003).
RN [5] {ECO:0000305}
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=9118222; DOI=10.1016/s0092-8674(00)81925-x;
RA van de Wetering M., Cavallo R.A., Dooijes D., van Beest M., van Es J.,
RA Loureiro J., Ypma A., Hursh D., Jones T., Bejsovec A., Peifer M.,
RA Mortin M., Clevers H.;
RT "Armadillo coactivates transcription driven by the product of the
RT Drosophila segment polarity gene dTCF.";
RL Cell 88:789-799(1997).
RN [6] {ECO:0000305}
RP FUNCTION.
RX PubMed=9039917; DOI=10.1038/385829a0;
RA Brunner E., Peter O., Schweizer L., Basler K.;
RT "Pangolin encodes a Lef-1 homologue that acts downstream of Armadillo to
RT transduce the Wingless signal in Drosophila.";
RL Nature 385:829-833(1997).
RN [7] {ECO:0000305}
RP FUNCTION, INTERACTION WITH ARM AND GRO, AND SUBCELLULAR LOCATION.
RX PubMed=9783586; DOI=10.1038/26982;
RA Cavallo R.A., Cox R.T., Moline M.M., Roose J., Polevoy G.A., Clevers H.,
RA Peifer M., Bejsovec A.;
RT "Drosophila Tcf and Groucho interact to repress Wingless signalling
RT activity.";
RL Nature 395:604-608(1998).
CC -!- FUNCTION: Segment polarity protein. Functions together with arm to
CC transduce the Wingless (Wg) signal in embryos and in developing adult
CC tissues. Acts as a transcriptional activator, but in the absence of
CC arm, it binds to gro and acts as a transcriptional repressor of wg-
CC responsive genes. {ECO:0000269|PubMed:9039917,
CC ECO:0000269|PubMed:9118222, ECO:0000269|PubMed:9783586}.
CC -!- SUBUNIT: Binds to the beta-catenin homolog arm or to gro.
CC {ECO:0000269|PubMed:9783586}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00267,
CC ECO:0000269|PubMed:9783586}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=5;
CC Name=J {ECO:0000269|PubMed:10731132};
CC IsoId=Q8IMA8-1; Sequence=Displayed;
CC Name=S;
CC IsoId=P91943-6; Sequence=External;
CC Name=A {ECO:0000269|PubMed:10731132}; Synonyms=C
CC {ECO:0000269|PubMed:10731132}, D {ECO:0000269|PubMed:10731132}, E
CC {ECO:0000269|PubMed:10731132}, F {ECO:0000269|PubMed:10731132}, G
CC {ECO:0000269|PubMed:10731132};
CC IsoId=P91943-3; Sequence=External;
CC Name=H {ECO:0000269|PubMed:10731132};
CC IsoId=P91943-4; Sequence=External;
CC Name=I {ECO:0000269|PubMed:10731132};
CC IsoId=P91943-5; Sequence=External;
CC -!- DISRUPTION PHENOTYPE: Flies exhibit a segment polarity phenotype and
CC altered expression of the wg target genes en and Ubx.
CC {ECO:0000269|PubMed:9118222}.
CC -!- SIMILARITY: Belongs to the TCF/LEF family. {ECO:0000255}.
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DR EMBL; AE014135; AAN06549.2; -; Genomic_DNA.
DR EMBL; BT029282; ABK30919.1; -; mRNA.
DR EMBL; AY312729; AAQ67543.1; -; Genomic_DNA.
DR EMBL; AY312730; AAQ67544.1; -; Genomic_DNA.
DR EMBL; AY312731; AAQ67545.1; -; Genomic_DNA.
DR EMBL; AY312732; AAQ67546.1; -; Genomic_DNA.
DR EMBL; AY312733; AAQ67547.1; -; Genomic_DNA.
DR EMBL; AY312734; AAQ67548.1; -; Genomic_DNA.
DR EMBL; AY312735; AAQ67549.1; -; Genomic_DNA.
DR EMBL; AY312736; AAQ67550.1; -; Genomic_DNA.
DR EMBL; AY312737; AAQ67551.1; -; Genomic_DNA.
DR EMBL; AY312738; AAQ67552.1; -; Genomic_DNA.
DR EMBL; AY312739; AAQ67553.1; -; Genomic_DNA.
DR EMBL; AY312740; AAQ67554.1; -; Genomic_DNA.
DR EMBL; AY312741; AAQ67555.1; -; Genomic_DNA.
DR EMBL; AY312742; AAQ67556.1; -; Genomic_DNA.
DR EMBL; AY312743; AAQ67557.1; -; Genomic_DNA.
DR EMBL; AY312744; AAQ67558.1; -; Genomic_DNA.
DR EMBL; AY312745; AAQ67559.1; -; Genomic_DNA.
DR EMBL; AY312746; AAQ67560.1; -; Genomic_DNA.
DR EMBL; AY312747; AAQ67561.1; -; Genomic_DNA.
DR EMBL; AY312748; AAQ67562.1; -; Genomic_DNA.
DR EMBL; AY312749; AAQ67563.1; -; Genomic_DNA.
DR EMBL; AY312750; AAQ67564.1; -; Genomic_DNA.
DR EMBL; AY312751; AAQ67565.1; -; Genomic_DNA.
DR EMBL; AY312752; AAQ67566.1; -; Genomic_DNA.
DR RefSeq; NP_726528.2; NM_166724.4. [Q8IMA8-1]
DR AlphaFoldDB; Q8IMA8; -.
DR SMR; Q8IMA8; -.
DR BioGRID; 68607; 71.
DR IntAct; Q8IMA8; 9.
DR STRING; 7227.FBpp0111569; -.
DR PaxDb; Q8IMA8; -.
DR PRIDE; Q8IMA8; -.
DR DNASU; 43769; -.
DR EnsemblMetazoa; FBtr0112657; FBpp0111569; FBgn0085432. [Q8IMA8-1]
DR GeneID; 43769; -.
DR CTD; 43769; -.
DR FlyBase; FBgn0085432; pan.
DR VEuPathDB; VectorBase:FBgn0085432; -.
DR eggNOG; KOG3248; Eukaryota.
DR GeneTree; ENSGT00940000168653; -.
DR HOGENOM; CLU_271719_0_0_1; -.
DR InParanoid; Q8IMA8; -.
DR PhylomeDB; Q8IMA8; -.
DR Reactome; R-DME-201722; Formation of the beta-catenin:TCF transactivating complex.
DR Reactome; R-DME-3769402; Deactivation of the beta-catenin transactivating complex.
DR Reactome; R-DME-4086398; Ca2+ pathway.
DR Reactome; R-DME-4641265; Repression of WNT target genes.
DR Reactome; R-DME-8853884; Transcriptional Regulation by VENTX.
DR Reactome; R-DME-8951430; RUNX3 regulates WNT signaling.
DR SignaLink; Q8IMA8; -.
DR BioGRID-ORCS; 43769; 0 hits in 3 CRISPR screens.
DR ChiTaRS; pan; fly.
DR GenomeRNAi; 43769; -.
DR Proteomes; UP000000803; Chromosome 4.
DR Bgee; FBgn0085432; Expressed in brain and 32 other tissues.
DR ExpressionAtlas; Q8IMA8; baseline and differential.
DR Genevisible; Q8IMA8; DM.
DR GO; GO:1990907; C:beta-catenin-TCF complex; IMP:FlyBase.
DR GO; GO:0005634; C:nucleus; IDA:FlyBase.
DR GO; GO:0005667; C:transcription regulator complex; IPI:FlyBase.
DR GO; GO:0001228; F:DNA-binding transcription activator activity, RNA polymerase II-specific; IDA:FlyBase.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IDA:FlyBase.
DR GO; GO:0019900; F:kinase binding; IPI:FlyBase.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IDA:FlyBase.
DR GO; GO:0001222; F:transcription corepressor binding; IPI:FlyBase.
DR GO; GO:0060070; P:canonical Wnt signaling pathway; IDA:FlyBase.
DR GO; GO:0090254; P:cell elongation involved in imaginal disc-derived wing morphogenesis; IMP:FlyBase.
DR GO; GO:0035293; P:chitin-based larval cuticle pattern formation; IGI:FlyBase.
DR GO; GO:0048546; P:digestive tract morphogenesis; IMP:FlyBase.
DR GO; GO:0009880; P:embryonic pattern specification; IMP:FlyBase.
DR GO; GO:0007507; P:heart development; IMP:FlyBase.
DR GO; GO:0007476; P:imaginal disc-derived wing morphogenesis; IMP:FlyBase.
DR GO; GO:0007500; P:mesodermal cell fate determination; TAS:FlyBase.
DR GO; GO:0045892; P:negative regulation of transcription, DNA-templated; IMP:UniProtKB.
DR GO; GO:0010628; P:positive regulation of gene expression; IMP:FlyBase.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IDA:FlyBase.
DR GO; GO:0045893; P:positive regulation of transcription, DNA-templated; IMP:UniProtKB.
DR GO; GO:0072091; P:regulation of stem cell proliferation; IMP:FlyBase.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; IMP:UniProtKB.
DR GO; GO:0007435; P:salivary gland morphogenesis; IMP:FlyBase.
DR GO; GO:0007367; P:segment polarity determination; IGI:FlyBase.
DR GO; GO:0035277; P:spiracle morphogenesis, open tracheal system; IMP:FlyBase.
DR Gene3D; 1.10.30.10; -; 1.
DR InterPro; IPR009071; HMG_box_dom.
DR InterPro; IPR036910; HMG_box_dom_sf.
DR InterPro; IPR028782; Pangolin-like.
DR InterPro; IPR024940; TCF/LEF.
DR PANTHER; PTHR10373; PTHR10373; 1.
DR PANTHER; PTHR10373:SF38; PTHR10373:SF38; 1.
DR Pfam; PF00505; HMG_box; 1.
DR SMART; SM00398; HMG; 1.
DR SUPFAM; SSF47095; SSF47095; 1.
DR PROSITE; PS50118; HMG_BOX_2; 1.
PE 1: Evidence at protein level;
KW Activator; Alternative splicing; Developmental protein; DNA-binding;
KW Nucleus; Reference proteome; Repressor; Segmentation polarity protein;
KW Transcription; Transcription regulation; Wnt signaling pathway.
FT CHAIN 1..1192
FT /note="Protein pangolin, isoform J"
FT /id="PRO_0000322557"
FT DNA_BIND 714..782
FT /note="HMG box"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00267"
FT REGION 691..713
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 790..812
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 847..916
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 955..986
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1135..1192
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 351..357
FT /note="Nuclear localization signal"
FT /evidence="ECO:0000255"
FT COMPBIAS 894..916
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 1192 AA; 132080 MW; 173ED73F8673DAA6 CRC64;
MPHCGTTNSE INLNSNELIS YQKKKSNEDL QKKHDKKCSI NLKTSQVPDG GFNNQYTQLT
ANKIEQYNCN DLDKVCIVPS SRSDGMEASF SELLLSPNKL ISQPWQTAEE IENWQNDSFR
QRNEMFSCIY TNSMLNQQPC SQQQLLATQL LYARLLRSQL AEREFHSNKF NMVHYSGSKK
TMLREDELLS TPSSQDNNNN IKLIKDIENS ISCVDPPLFE FSNVHQRAEQ KNKQDDKNCY
SPKLKSNKEA LDGYDLQHTC DFIREQKNIL IDIKKKLDNL SDSSGKFRKR LSVRQSHIEV
NNGSNSALEE SVRRQLNGNR KSIENLLEEV KRLYNQWSSA ELYYVRSLQR LGLPSEEDGE
YTSTPTHTIM ALAAIALSNE SILPQKTNAV HSKIPDVESE SDFFLTSAKP KTLVEIEDII
LQLASSVNMH QSSSASTPHE SYSDSVKSDC EESNSAPTCI WHSTRQTFRH KKDVEPCSTA
AEIILEYASL SSSSNIETSR LLTSASNLTD VTENYNVTTQ LPIVFNYNRE SSAESIVTDP
LLVPEFSTAP STPSTGSNSG CSTGMVSGIF GLSQNRRKQR LARHIETLTT NSFKSNAIRG
NVDNEITNQL KTSPSIRALP TENVSHLITK TLSSANASLT AQELSITNLF KERLCALQGN
AGSMKTEIFP MIDAPYDLSI GSKTKHMNLE AKHTSNAQSN ESKETTNDKK KPHIKKPLNA
FMLYMKEMRA KVVAECTLKE SAAINQILGR RWHELSREEQ SKYYEKARQE RQLHMELYPG
WSARDNYGYV SKKKKRKKDR STTDSGGNNM KKCRARFGLD QQSQWCKPCR RKKKCIRYME
ALNGNGPAED GSCFDEHGSQ LSDDDEDDYD DDKLGGSCGS ADETNKIEDE DSESLNQSMP
SPGCLSGLSS LQSPSTTMSL ASPLNMNANS ATNVIFPASS NALLIVGADQ PTAQQRPTLV
STSGSSSGST SSISTTPNTS STVSPVTCMT GPCLGSSQER AMMLGNRFSH LGMGLSPPVV
STSTSKSEPF FKPHPTVCNN PIFALPSIGN CSLNISSMPN TSRNPIGANP RDINNPLSIN
QLTKRREYKN VELIEASESK TIVAHAATSI IQHVAVNGYH ANHSLLNSNL GHLHHQLNNR
TENPNRSEQT MLSVSNHSVN SSECHKESDS QAIVSSNPPN AGSSDNGVIS VS
