PANS1_ARATH
ID PANS1_ARATH Reviewed; 193 AA.
AC Q9LJG6; Q84WK8; Q8LFX6;
DT 30-NOV-2016, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 03-AUG-2022, entry version 102.
DE RecName: Full=Protein PATRONUS 1 {ECO:0000303|Ref.5};
DE AltName: Full=Protein COPPER MODIFIED RESISTANCE 1 {ECO:0000303|PubMed:24134393};
GN Name=PANS1 {ECO:0000303|Ref.5};
GN Synonyms=CMR1 {ECO:0000303|PubMed:24134393};
GN OrderedLocusNames=At3g14190 {ECO:0000312|Araport:AT3G14190,
GN ECO:0000312|EMBL:AEE75484.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702 {ECO:0000312|EMBL:BAB02986.1};
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10907853; DOI=10.1093/dnares/7.3.217;
RA Kaneko T., Katoh T., Sato S., Nakamura Y., Asamizu E., Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 3. II. Sequence
RT features of the 4,251,695 bp regions covered by 90 P1, TAC and BAC
RT clones.";
RL DNA Res. 7:217-221(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA Feldmann K.A.;
RT "Full-length cDNA from Arabidopsis thaliana.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP FUNCTION, INDUCTION, TISSUE SPECIFICITY, DISRUPTION PHENOTYPE, INTERACTION
RP WITH CDC27B AND CDC20-1, MUTAGENESIS OF 14-ASP--ASN-16 AND 46-ARG--LEU-49,
RP AND DOMAIN.
RX PubMed=24206843; DOI=10.1016/j.cub.2013.08.036;
RA Cromer L., Jolivet S., Horlow C., Chelysheva L., Heyman J., De Jaeger G.,
RA Koncz C., De Veylder L., Mercier R.;
RT "Centromeric cohesion is protected twice at meiosis, by SHUGOSHINs at
RT anaphase I and by PATRONUS at interkinesis.";
RL Curr. Biol. 23:2090-2099(2013).
RN [7]
RP FUNCTION, DISRUPTION PHENOTYPE, AND SUBCELLULAR LOCATION.
RX PubMed=24134393; DOI=10.1111/nph.12554;
RA Juraniec M., Lequeux H., Hermans C., Willems G., Nordborg M.,
RA Schneeberger K., Salis P., Vromant M., Lutts S., Verbruggen N.;
RT "Towards the discovery of novel genetic component involved in stress
RT resistance in Arabidopsis thaliana.";
RL New Phytol. 201:810-824(2014).
RN [8]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=24506176; DOI=10.1111/tpj.12432;
RA Zamariola L., De Storme N., Vannerum K., Vandepoele K., Armstrong S.J.,
RA Franklin F.C., Geelen D.;
RT "SHUGOSHINs and PATRONUS protect meiotic centromere cohesion in Arabidopsis
RT thaliana.";
RL Plant J. 77:782-794(2014).
RN [9]
RP FUNCTION, DISRUPTION PHENOTYPE, SUBCELLULAR LOCATION, TISSUE SPECIFICITY,
RP AND INDUCTION.
RX PubMed=26272661; DOI=10.1186/s12870-015-0558-6;
RA Singh D.K., Spillane C., Siddiqi I.;
RT "PATRONUS1 is expressed in meiotic prophase I to regulate centromeric
RT cohesion in Arabidopsis and shows synthetic lethality with OSD1.";
RL BMC Plant Biol. 15:201-201(2015).
RN [10]
RP FUNCTION, TISSUE SPECIFICITY, INDUCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=26261921; DOI=10.1111/nph.13589;
RA Juraniec M., Heyman J., Schubert V., Salis P., De Veylder L.,
RA Verbruggen N.;
RT "Arabidopsis COPPER MODIFIED RESISTANCE1/PATRONUS1 is essential for growth
RT adaptation to stress and required for mitotic onset control.";
RL New Phytol. 209:177-191(2016).
CC -!- FUNCTION: Required for the maintenance of centromeric cohesion during
CC interkinesis, until meiosis II (PubMed:24206843, PubMed:26272661).
CC Required for regular configuration and segregation of sister chromatids
CC in meiosis II (PubMed:24506176). Also required for centromere cohesion
CC during meiosis I (PubMed:26272661). Involved in spindle organization at
CC the end of telophase I and in meiosis II (PubMed:24506176). Required to
CC prevent precocious release of pericentromeric cohesins during meiosis,
CC but not for cohesion establishment and monopolar orientation of
CC kinetochores at meiosis I (PubMed:24206843). Involved also in somatic
CC development (PubMed:24206843). Regulates mitotic cell division and
CC ploidy stability in somatic cell types (PubMed:24506176). May be
CC involved in the organization of microtubules dynamics
CC (PubMed:24506176). Involved in abiotic stresses and mono- or divalent
CC ions tolerance and may play a role in maintaining meristematic activity
CC under saline conditions (PubMed:24134393). PANS1 and GIG1 are part of a
CC network linking centromere cohesion and cell cycle progression through
CC control of APC/C activity (PubMed:26272661). Regulates the number of
CC dividing cells in root meristem and is necessary for the anaphase onset
CC control through an APC/C-mediated pathway (PubMed:26261921). Involved
CC in maintaining correct chromosome arm cohesion under stress conditions
CC (PubMed:26261921). {ECO:0000269|PubMed:24134393,
CC ECO:0000269|PubMed:24206843, ECO:0000269|PubMed:24506176,
CC ECO:0000269|PubMed:26261921, ECO:0000269|PubMed:26272661}.
CC -!- SUBUNIT: Interacts directly with the anaphase promoting
CC complex/cyclosome (APC/C) through the CDC27B and CDC20-1 subunits.
CC {ECO:0000269|PubMed:24206843}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:24134393,
CC ECO:0000269|PubMed:26272661}. Cytoplasm {ECO:0000269|PubMed:24134393}.
CC Note=Excluded from the nucleolus. {ECO:0000269|PubMed:26272661}.
CC -!- TISSUE SPECIFICITY: Expressed in somatic and reproductive tissues
CC (PubMed:24206843). Expressed in inflorescence, young buds, roots and
CC basal portion of young leaves (PubMed:26272661). Expressed in
CC proliferating cells such as apical meristems of roots and shoots,
CC expanding cotyledons and leaves, root vascular tissues, and in stomatal
CC precursor cells (PubMed:26261921). {ECO:0000269|PubMed:24206843,
CC ECO:0000269|PubMed:26261921, ECO:0000269|PubMed:26272661}.
CC -!- INDUCTION: Cell cycle regulated with a peak at late M/early G1 phase
CC mitosis (PubMed:24206843). Peak of expression during metaphase
CC (PubMed:26261921). Strongly expressed up to mid-prophase I and
CC decreases during late prophase (PubMed:26272661). Not induced by stress
CC (PubMed:26261921). {ECO:0000269|PubMed:26261921,
CC ECO:0000269|PubMed:26272661, ECO:0000305|PubMed:24206843}.
CC -!- DOMAIN: The DEN-box is not essential for the meiotic function.
CC {ECO:0000269|PubMed:24206843}.
CC -!- DISRUPTION PHENOTYPE: Reduced fertility when homozygous
CC (PubMed:24206843, PubMed:24506176). High level of gametophytic
CC aneuploidy (PubMed:24506176, PubMed:26272661). Occurrence of split
CC sister centromeres at metaphase I (PubMed:26272661). Pans1 and pans2
CC double mutants are lethal when homozygous (PubMed:24206843). Increased
CC mono- or divalent ions sensitivity resulting in primary root growth
CC inhibition and increased lateral root density (PubMed:24134393).
CC Hypersensitivity to microtubule-depolymerizing drugs and higher
CC frequency of anaphase bridges under stress conditions
CC (PubMed:26261921). {ECO:0000269|PubMed:24134393,
CC ECO:0000269|PubMed:24206843, ECO:0000269|PubMed:24506176,
CC ECO:0000269|PubMed:26261921, ECO:0000269|PubMed:26272661}.
CC -!- MISCELLANEOUS: Patronus is Latin for protector.
CC {ECO:0000305|PubMed:24206843}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AP000600; BAB02986.1; -; Genomic_DNA.
DR EMBL; CP002686; AEE75484.1; -; Genomic_DNA.
DR EMBL; CP002686; ANM65516.1; -; Genomic_DNA.
DR EMBL; BT003111; AAO24543.1; -; mRNA.
DR EMBL; AK228197; BAF00151.1; -; mRNA.
DR EMBL; AY084592; AAM61157.1; -; mRNA.
DR RefSeq; NP_001327476.1; NM_001338112.1.
DR RefSeq; NP_566480.1; NM_112275.3.
DR AlphaFoldDB; Q9LJG6; -.
DR STRING; 3702.AT3G14190.1; -.
DR PaxDb; Q9LJG6; -.
DR PRIDE; Q9LJG6; -.
DR EnsemblPlants; AT3G14190.1; AT3G14190.1; AT3G14190.
DR EnsemblPlants; AT3G14190.2; AT3G14190.2; AT3G14190.
DR GeneID; 820636; -.
DR Gramene; AT3G14190.1; AT3G14190.1; AT3G14190.
DR Gramene; AT3G14190.2; AT3G14190.2; AT3G14190.
DR KEGG; ath:AT3G14190; -.
DR Araport; AT3G14190; -.
DR TAIR; locus:2087482; AT3G14190.
DR eggNOG; ENOG502R1GX; Eukaryota.
DR HOGENOM; CLU_1423353_0_0_1; -.
DR InParanoid; Q9LJG6; -.
DR OMA; KYDTEMM; -.
DR OrthoDB; 1331402at2759; -.
DR PhylomeDB; Q9LJG6; -.
DR PRO; PR:Q9LJG6; -.
DR Proteomes; UP000006548; Chromosome 3.
DR ExpressionAtlas; Q9LJG6; baseline and differential.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005730; C:nucleolus; IDA:TAIR.
DR GO; GO:0005634; C:nucleus; IDA:TAIR.
DR GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
DR GO; GO:0007059; P:chromosome segregation; IEA:UniProtKB-KW.
DR GO; GO:0051321; P:meiotic cell cycle; IEA:UniProtKB-KW.
DR GO; GO:0034096; P:positive regulation of maintenance of meiotic sister chromatid cohesion; IMP:TAIR.
DR GO; GO:2000711; P:positive regulation of maintenance of meiotic sister chromatid cohesion, centromeric; IMP:TAIR.
DR GO; GO:0007346; P:regulation of mitotic cell cycle; IMP:TAIR.
DR InterPro; IPR039326; Patronus.
DR PANTHER; PTHR35125; PTHR35125; 1.
PE 1: Evidence at protein level;
KW Cell cycle; Cell division; Chromosome partition; Cytoplasm; Meiosis;
KW Mitosis; Nucleus; Reference proteome.
FT CHAIN 1..193
FT /note="Protein PATRONUS 1"
FT /id="PRO_0000438544"
FT MOTIF 14..16
FT /note="DEN-box"
FT /evidence="ECO:0000305"
FT MOTIF 46..49
FT /note="D-box"
FT /evidence="ECO:0000305"
FT MUTAGEN 14..16
FT /note="DEN->AAA: No effect on the interaction with CDC27B,
FT but strongly decreased interaction with CDC20-1. Loss of
FT interaction with CDC20-1 and lethality; when associated
FT with 46-LKAV-49."
FT /evidence="ECO:0000269|PubMed:24206843"
FT MUTAGEN 46..49
FT /note="RKAL->LKAV: No effect on the interaction with
FT CDC27B, but strongly decreased interaction with CDC20-1.
FT Loss of interaction with CDC20-1 and lethality; when
FT associated with 14-AAA-16."
FT /evidence="ECO:0000269|PubMed:24206843"
FT CONFLICT 43
FT /note="G -> V (in Ref. 5; AAM61157)"
FT /evidence="ECO:0000305"
FT CONFLICT 77
FT /note="K -> E (in Ref. 3; AAO24543 and 4; BAF00151)"
FT /evidence="ECO:0000305"
FT CONFLICT 77
FT /note="K -> KD (in Ref. 5; AAM61157)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 193 AA; 21733 MW; DC8718A166F75161 CRC64;
MANMNALQQM IFPDENAPIH RKKSVTAASV KSKGTVLGQK KPGGARKALN DITNKSGIHA
KAAASSKNKQ IASAAVKEID IAGERFLHDH SKCIKEQQNL WDDHYSADLM LLHHGSSIKE
KHLNWDIEKM DAKDDLTYEE PEEMASPKFS DWLKNSTPWR SPIRHGSMMP STPLAWRFDS
CEFTLKEDSD DLF
