ID   PANSN_PANAR             Reviewed;          66 AA.
AC   F6KSI8; A0A0S2XGP2;
DT   01-MAY-2013, integrated into UniProtKB/Swiss-Prot.
DT   10-OCT-2018, sequence version 2.
DT   25-MAY-2022, entry version 13.
DE   RecName: Full=Panusin {ECO:0000303|PubMed:27616720};
DE   AltName: Full=Defensin-like peptide 7 {ECO:0000303|PubMed:22885029};
DE            Short=PaD7 {ECO:0000303|PubMed:22885029};
DE   Flags: Precursor;
OS   Panulirus argus (Caribbean spiny lobster) (Palinurus argus).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Crustacea; Multicrustacea;
OC   Malacostraca; Eumalacostraca; Eucarida; Decapoda; Pleocyemata; Achelata;
OC   Palinuroidea; Palinuridae; Panulirus.
OX   NCBI_TaxID=6737;
RN   [1] {ECO:0000312|EMBL:AEE69605.1}
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   TISSUE=Hemolymph {ECO:0000269|PubMed:22885029};
RX   PubMed=22885029; DOI=10.1016/j.fsi.2012.07.013;
RA   Montero-Alejo V., Acosta-Alba J., Perdomo-Morales R., Perera E.,
RA   Hernandez-Rodriguez E.W., Estrada M.P., Porto-Verdecia M.;
RT   "Defensin like peptide from Panulirus argus relates structurally with beta
RT   defensin from vertebrates.";
RL   Fish Shellfish Immunol. 33:872-879(2012).
RN   [2] {ECO:0000305}
RP   NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 27-48, FUNCTION, SUBUNIT,
RP   MASS SPECTROMETRY, PRESENCE OF DISULFIDE BONDS, AND AMIDATION AT TYR-65.
RC   TISSUE=Hemocyte {ECO:0000269|PubMed:27616720};
RX   PubMed=27616720; DOI=10.1016/j.dci.2016.09.002;
RA   Montero-Alejo V., Corzo G., Porro-Suardiaz J., Pardo-Ruiz Z., Perera E.,
RA   Rodriguez-Viera L., Sanchez-Diaz G., Hernandez-Rodriguez E.W., Alvarez C.,
RA   Peigneur S., Tytgat J., Perdomo-Morales R.;
RT   "Panusin represents a new family of beta-defensin-like peptides in
RT   invertebrates.";
RL   Dev. Comp. Immunol. 67:310-321(2017).
CC   -!- FUNCTION: Antimicrobial peptide. Has antibacterial activity against
CC       Gram-positive bacteria S.aureus ATCC 29737 and B.subtilis ATCC 6633 as
CC       well as against Gram-negative bacteria E.coli ATCC 10536 and
CC       K.pneumoniae ATCC 10031. {ECO:0000269|PubMed:27616720}.
CC   -!- SUBUNIT: Forms dimers and higher-order oligomers.
CC       {ECO:0000305|PubMed:27616720}.
CC   -!- PTM: Contains 3 disulfide bonds. {ECO:0000269|PubMed:27616720}.
CC   -!- MASS SPECTROMETRY: Mass=4260.3; Method=Electrospray;
CC       Evidence={ECO:0000269|PubMed:27616720};
CC   -!- SIMILARITY: Belongs to the beta-defensin family. {ECO:0000255}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; HQ599307; AEE69605.1; -; mRNA.
DR   EMBL; KT253226; ALQ10737.1; -; mRNA.
DR   AlphaFoldDB; F6KSI8; -.
DR   SMR; F6KSI8; -.
DR   GO; GO:0042742; P:defense response to bacterium; IEA:UniProtKB-KW.
PE   1: Evidence at protein level;
KW   Amidation; Antibiotic; Antimicrobial; Defensin; Direct protein sequencing;
KW   Disulfide bond; Signal.
FT   SIGNAL          1..22
FT                   /evidence="ECO:0000255"
FT   PROPEP          23..26
FT                   /evidence="ECO:0000305|PubMed:27616720"
FT                   /id="PRO_0000422178"
FT   PEPTIDE         27..65
FT                   /note="Panusin"
FT                   /evidence="ECO:0000269|PubMed:27616720"
FT                   /id="PRO_0000445467"
FT   MOD_RES         65
FT                   /note="Tyrosine amide"
FT                   /evidence="ECO:0000269|PubMed:27616720"
FT   DISULFID        32..54
FT                   /evidence="ECO:0000303|PubMed:27616720"
FT   DISULFID        39..61
FT                   /evidence="ECO:0000303|PubMed:27616720"
FT   DISULFID        44..60
FT                   /evidence="ECO:0000303|PubMed:27616720"
FT   CONFLICT        6..7
FT                   /note="VL -> IV (in Ref. 1; AEE69605)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        63..66
FT                   /note="RSYG -> HAYV (in Ref. 1; AEE69605)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   66 AA;  7102 MW;  919077AD8441E1DF CRC64;
     MKTKAVLMLM LLVLVAATLV QGEPEPSYVG DCGSNGGSCV SSYCPYGNRL NYFCPLGRTC
     CRRSYG