PAP1_ARATH
ID PAP1_ARATH Reviewed; 318 AA.
AC O81439;
DT 26-APR-2005, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1998, sequence version 1.
DT 25-MAY-2022, entry version 134.
DE RecName: Full=Probable plastid-lipid-associated protein 1, chloroplastic;
DE Short=AtPap1;
DE AltName: Full=Fibrillin-1a;
DE AltName: Full=Plastoglobulin 35;
DE Short=AtPGL35;
DE Flags: Precursor;
GN Name=PAP1; Synonyms=FBN1a, FIB1a, PGL35; OrderedLocusNames=At4g04020;
GN ORFNames=T24H24.16;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617198; DOI=10.1038/47134;
RA Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T.,
RA Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B.,
RA Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M.,
RA de Simone V., Obermaier B., Mache R., Mueller M., Kreis M., Delseny M.,
RA Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D.,
RA Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J.,
RA Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B.,
RA Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J.,
RA Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R.,
RA Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M.,
RA Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P.,
RA Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S.,
RA Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C.,
RA Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J.,
RA Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S.,
RA Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A.,
RA Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M.,
RA Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D.,
RA Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E.,
RA Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S.,
RA Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R.,
RA Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M.,
RA Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E.,
RA Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P.,
RA Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K.,
RA Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K.,
RA de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K.,
RA Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M.,
RA Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G.,
RA Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K.,
RA Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K.,
RA Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W.,
RA Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H.,
RA Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B.,
RA Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J.,
RA Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K.,
RA O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N.,
RA Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A.,
RA Martienssen R., McCombie W.R.;
RT "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.";
RL Nature 402:769-777(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA Feldmann K.A.;
RT "Full-length cDNA from Arabidopsis thaliana.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP PROTEIN SEQUENCE OF 56-62, AND SUBCELLULAR LOCATION.
RX PubMed=11719511; DOI=10.1074/jbc.m108575200;
RA Schubert M., Petersson U.A., Haas B.J., Funk C., Schroeder W.P.,
RA Kieselbach T.;
RT "Proteome map of the chloroplast lumen of Arabidopsis thaliana.";
RL J. Biol. Chem. 277:8354-8365(2002).
RN [6]
RP SUBCELLULAR LOCATION.
RC STRAIN=cv. Col-2;
RX PubMed=16414959; DOI=10.1074/jbc.m511939200;
RA Vidi P.-A., Kanwischer M., Baginsky S., Austin J.R., Csucs G., Doermann P.,
RA Kessler F., Brehelin C.;
RT "Tocopherol cyclase (VTE1) localization and vitamin E accumulation in
RT chloroplast plastoglobule lipoprotein particles.";
RL J. Biol. Chem. 281:11225-11234(2006).
RN [7]
RP IDENTIFICATION BY MASS SPECTROMETRY, AND SUBCELLULAR LOCATION [LARGE SCALE
RP ANALYSIS].
RC STRAIN=cv. Columbia;
RX PubMed=16461379; DOI=10.1104/pp.105.076083;
RA Ytterberg A.J., Peltier J.-B., van Wijk K.J.;
RT "Protein profiling of plastoglobules in chloroplasts and chromoplasts. A
RT surprising site for differential accumulation of metabolic enzymes.";
RL Plant Physiol. 140:984-997(2006).
RN [8]
RP FUNCTION, INDUCTION BY ABSCISIC ACID, AND INTERACTION WITH ABI2.
RX PubMed=16571665; DOI=10.1073/pnas.0501720103;
RA Yang Y., Sulpice R., Himmelbach A., Meinhard M., Christmann A., Grill E.;
RT "Fibrillin expression is regulated by abscisic acid response regulators and
RT is involved in abscisic acid-mediated photoprotection.";
RL Proc. Natl. Acad. Sci. U.S.A. 103:6061-6066(2006).
RN [9]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-57, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=19376835; DOI=10.1104/pp.109.138677;
RA Reiland S., Messerli G., Baerenfaller K., Gerrits B., Endler A.,
RA Grossmann J., Gruissem W., Baginsky S.;
RT "Large-scale Arabidopsis phosphoproteome profiling reveals novel
RT chloroplast kinase substrates and phosphorylation networks.";
RL Plant Physiol. 150:889-903(2009).
RN [10]
RP FUNCTION.
RX PubMed=19906042; DOI=10.1111/j.1365-313x.2009.04067.x;
RA Youssef A., Laizet Y., Block M.A., Marechal E., Alcaraz J.P., Larson T.R.,
RA Pontier D., Gaffe J., Kuntz M.;
RT "Plant lipid-associated fibrillin proteins condition jasmonate production
RT under photosynthetic stress.";
RL Plant J. 61:436-445(2010).
RN [11]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=21571574; DOI=10.1016/j.tplants.2011.03.014;
RA Singh D.K., McNellis T.W.;
RT "Fibrillin protein function: the tip of the iceberg?";
RL Trends Plant Sci. 16:432-441(2011).
RN [12]
RP IDENTIFICATION BY MASS SPECTROMETRY, AND SUBCELLULAR LOCATION [LARGE SCALE
RP ANALYSIS].
RC STRAIN=cv. Columbia;
RX PubMed=22274653; DOI=10.1104/pp.111.193144;
RA Lundquist P.K., Poliakov A., Bhuiyan N.H., Zybailov B., Sun Q.,
RA van Wijk K.J.;
RT "The functional network of the Arabidopsis plastoglobule proteome based on
RT quantitative proteomics and genome-wide coexpression analysis.";
RL Plant Physiol. 158:1172-1192(2012).
RN [13]
RP SUBCELLULAR LOCATION.
RC STRAIN=cv. Columbia;
RX PubMed=23086498; DOI=10.1007/s11103-012-9977-z;
RA Shanmugabalaji V., Besagni C., Piller L.E., Douet V., Ruf S., Bock R.,
RA Kessler F.;
RT "Dual targeting of a mature plastoglobulin/fibrillin fusion protein to
RT chloroplast plastoglobules and thylakoids in transplastomic tobacco
RT plants.";
RL Plant Mol. Biol. 81:13-25(2013).
RN [14]
RP TISSUE SPECIFICITY, SUBCELLULAR LOCATION, AND DEVELOPMENTAL STAGE.
RC STRAIN=cv. Columbia;
RX PubMed=23602096; DOI=10.1016/j.plantsci.2013.02.008;
RA Suzuki T., Tsunekawa S., Koizuka C., Yamamoto K., Imamura J., Nakamura K.,
RA Ishiguro S.;
RT "Development and disintegration of tapetum-specific lipid-accumulating
RT organelles, elaioplasts and tapetosomes, in Arabidopsis thaliana and
RT Brassica napus.";
RL Plant Sci. 207:25-36(2013).
CC -!- FUNCTION: Probably involved in light/cold stress-related jasmonate (JA)
CC biosynthesis. Contributes to the protection of photosystem II (PSII)
CC against light stress. {ECO:0000269|PubMed:16571665,
CC ECO:0000269|PubMed:19906042}.
CC -!- SUBUNIT: Interacts (via N-terminus) with ABI2.
CC {ECO:0000269|PubMed:16571665}.
CC -!- INTERACTION:
CC O81439; O04719: ABI2; NbExp=5; IntAct=EBI-962363, EBI-537680;
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast, plastoglobule
CC {ECO:0000269|PubMed:16414959, ECO:0000269|PubMed:16461379,
CC ECO:0000269|PubMed:22274653}. Plastid, chloroplast thylakoid.
CC Note=Located in the elaioplast, a tapetum-specific plastidial lipid
CC organelle.
CC -!- TISSUE SPECIFICITY: Expressed in flower buds. Detected in tapetal
CC cells, endothecium and connective in anthers and in subepidermal cells
CC in filaments. {ECO:0000269|PubMed:23602096}.
CC -!- DEVELOPMENTAL STAGE: Expressed throughout anther development.
CC {ECO:0000269|PubMed:23602096}.
CC -!- INDUCTION: Up-regulated by abscisic acid.
CC {ECO:0000269|PubMed:16571665}.
CC -!- MISCELLANEOUS: Simultaneous down-regulation of PAP1, PAP2 and PAP3
CC leads to impaired long-term acclimation to environmental constraint,
CC namely photooxidative stress imposed by high light combined with cold.
CC {ECO:0000305|PubMed:19906042}.
CC -!- SIMILARITY: Belongs to the PAP/fibrillin family. {ECO:0000305}.
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DR EMBL; AF075598; AAC28198.1; -; Genomic_DNA.
DR EMBL; AL161499; CAB77870.1; -; Genomic_DNA.
DR EMBL; CP002687; AEE82363.1; -; Genomic_DNA.
DR EMBL; AY120766; AAM53324.1; -; mRNA.
DR EMBL; BT000148; AAN15467.1; -; mRNA.
DR EMBL; AY085330; AAM62561.1; -; mRNA.
DR PIR; T01472; T01472.
DR RefSeq; NP_192311.1; NM_116640.5.
DR AlphaFoldDB; O81439; -.
DR SMR; O81439; -.
DR BioGRID; 11027; 14.
DR DIP; DIP-37456N; -.
DR IntAct; O81439; 15.
DR MINT; O81439; -.
DR STRING; 3702.AT4G04020.1; -.
DR iPTMnet; O81439; -.
DR PaxDb; O81439; -.
DR PRIDE; O81439; -.
DR ProteomicsDB; 236839; -.
DR EnsemblPlants; AT4G04020.1; AT4G04020.1; AT4G04020.
DR GeneID; 825714; -.
DR Gramene; AT4G04020.1; AT4G04020.1; AT4G04020.
DR KEGG; ath:AT4G04020; -.
DR Araport; AT4G04020; -.
DR TAIR; locus:2136627; AT4G04020.
DR eggNOG; ENOG502QS2T; Eukaryota.
DR HOGENOM; CLU_045041_1_0_1; -.
DR OMA; VHRPDFK; -.
DR OrthoDB; 1037321at2759; -.
DR PhylomeDB; O81439; -.
DR PRO; PR:O81439; -.
DR Proteomes; UP000006548; Chromosome 4.
DR ExpressionAtlas; O81439; baseline and differential.
DR Genevisible; O81439; AT.
DR GO; GO:0009507; C:chloroplast; HDA:TAIR.
DR GO; GO:0009570; C:chloroplast stroma; HDA:TAIR.
DR GO; GO:0009534; C:chloroplast thylakoid; HDA:TAIR.
DR GO; GO:0009535; C:chloroplast thylakoid membrane; HDA:TAIR.
DR GO; GO:0005829; C:cytosol; HDA:TAIR.
DR GO; GO:0005634; C:nucleus; HDA:TAIR.
DR GO; GO:0009536; C:plastid; IDA:TAIR.
DR GO; GO:0010287; C:plastoglobule; HDA:TAIR.
DR GO; GO:0010319; C:stromule; IDA:TAIR.
DR GO; GO:0009579; C:thylakoid; HDA:TAIR.
DR GO; GO:0031977; C:thylakoid lumen; HDA:TAIR.
DR GO; GO:0010205; P:photoinhibition; IMP:TAIR.
DR GO; GO:0009737; P:response to abscisic acid; IEP:TAIR.
DR GO; GO:0009409; P:response to cold; IEP:TAIR.
DR InterPro; IPR039633; PAP.
DR InterPro; IPR006843; PAP/fibrillin_dom.
DR PANTHER; PTHR31906; PTHR31906; 1.
DR Pfam; PF04755; PAP_fibrillin; 1.
PE 1: Evidence at protein level;
KW Chloroplast; Direct protein sequencing; Phosphoprotein; Plastid;
KW Reference proteome; Thylakoid; Transit peptide.
FT TRANSIT 1..55
FT /note="Chloroplast"
FT /evidence="ECO:0000255"
FT CHAIN 56..318
FT /note="Probable plastid-lipid-associated protein 1,
FT chloroplastic"
FT /id="PRO_0000023208"
FT MOD_RES 57
FT /note="Phosphothreonine"
FT /evidence="ECO:0007744|PubMed:19376835"
SQ SEQUENCE 318 AA; 34948 MW; 8BD08F386AF0AE48 CRC64;
MATVPLFTQF PCKTLNPSSS NTKHQSKSPI LLPINSINRR SEIGVSVHRP DFKIRATDID
DEWGQDGVER VFASSSTVSV ADKAIESVEE TERLKRSLAD SLYGTDRGLS VSSDTRAEIS
ELITQLESKN PTPAPNEALF LLNGKWILAY TSFVGLFPLL SRRIEPLVKV DEISQTIDSD
SFTVQNSVRF AGPFSTTSFS TNAKFEIRSP KRVQIKFEQG VIGTPQLTDS IEIPESVEVL
GQKIDLNPIK GLLTSVQDTA SSVARTISNQ PPLKFSLPSD NTQSWLLTTY LDKDLRISRG
DGGSVYVLIK EGSSLLNP
