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ASNC_ECOLI
ID   ASNC_ECOLI              Reviewed;         152 AA.
AC   P0ACI6; P03809; Q2M861;
DT   21-JUL-1986, integrated into UniProtKB/Swiss-Prot.
DT   21-JUL-1986, sequence version 1.
DT   25-MAY-2022, entry version 131.
DE   RecName: Full=Regulatory protein AsnC;
GN   Name=asnC; OrderedLocusNames=b3743, JW3721;
OS   Escherichia coli (strain K12).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=83333;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=K12;
RX   PubMed=6117826; DOI=10.1093/nar/9.18.4669;
RA   Nakamura M., Yamada M., Hirota Y., Sugimoto K., Oka A., Takanami M.;
RT   "Nucleotide sequence of the asnA gene coding for asparagine synthetase of
RT   E. coli K-12.";
RL   Nucleic Acids Res. 9:4669-4676(1981).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=K12;
RX   PubMed=6357950; DOI=10.1016/0378-1119(83)90087-2;
RA   Buhk H.-J., Messer W.;
RT   "The replication origin region of Escherichia coli: nucleotide sequence and
RT   functional units.";
RL   Gene 24:265-279(1983).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / MG1655 / ATCC 47076;
RX   PubMed=7686882; DOI=10.1006/geno.1993.1230;
RA   Burland V.D., Plunkett G. III, Daniels D.L., Blattner F.R.;
RT   "DNA sequence and analysis of 136 kilobases of the Escherichia coli genome:
RT   organizational symmetry around the origin of replication.";
RL   Genomics 16:551-561(1993).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / MG1655 / ATCC 47076;
RX   PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA   Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA   Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA   Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA   Shao Y.;
RT   "The complete genome sequence of Escherichia coli K-12.";
RL   Science 277:1453-1462(1997).
RN   [5]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX   PubMed=16738553; DOI=10.1038/msb4100049;
RA   Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA   Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT   "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT   and W3110.";
RL   Mol. Syst. Biol. 2:E1-E5(2006).
RN   [6]
RP   FUNCTION.
RX   PubMed=3909107; DOI=10.1093/nar/13.24.8797;
RA   de Wind N., de Jong M., Meijer M., Stuitje A.R.;
RT   "Site-directed mutagenesis of the Escherichia coli chromosome near oriC:
RT   identification and characterization of asnC, a regulatory element in E.
RT   coli asparagine metabolism.";
RL   Nucleic Acids Res. 13:8797-8811(1985).
RN   [7]
RP   FUNCTION.
RX   PubMed=2864330; DOI=10.1128/jb.164.1.310-315.1985;
RA   Koelling R., Lother H.;
RT   "AsnC: an autogenously regulated activator of asparagine synthetase A
RT   transcription in Escherichia coli.";
RL   J. Bacteriol. 164:310-315(1985).
RN   [8]
RP   FUNCTION.
RX   PubMed=2836709; DOI=10.1007/bf00322450;
RA   Koelling R., Gielow A., Seufert W., Kuecherer C., Messer W.;
RT   "AsnC, a multifunctional regulator of genes located around the replication
RT   origin of Escherichia coli, oriC.";
RL   Mol. Gen. Genet. 212:99-104(1988).
CC   -!- FUNCTION: Activator of asnA transcription; autogenous regulator of its
CC       own transcription; and repressor of the expression of gidA at a post-
CC       transcriptional level. {ECO:0000269|PubMed:2836709,
CC       ECO:0000269|PubMed:2864330, ECO:0000269|PubMed:3909107}.
CC   -!- INTERACTION:
CC       P0ACI6; P31658: hchA; NbExp=2; IntAct=EBI-1133670, EBI-909144;
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DR   EMBL; V00263; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; K00826; AAA24252.1; -; Genomic_DNA.
DR   EMBL; L10328; AAA62095.1; -; Genomic_DNA.
DR   EMBL; U00096; AAC76766.1; -; Genomic_DNA.
DR   EMBL; AP009048; BAE77545.1; -; Genomic_DNA.
DR   PIR; A04434; QQECE1.
DR   RefSeq; NP_418199.1; NC_000913.3.
DR   RefSeq; WP_000432970.1; NZ_STEB01000015.1.
DR   RefSeq; YP_006952152.1; NC_019049.1.
DR   PDB; 2CG4; X-ray; 2.40 A; A/B=1-152.
DR   PDBsum; 2CG4; -.
DR   AlphaFoldDB; P0ACI6; -.
DR   SMR; P0ACI6; -.
DR   BioGRID; 4263262; 98.
DR   BioGRID; 852561; 1.
DR   DIP; DIP-9178N; -.
DR   IntAct; P0ACI6; 1.
DR   STRING; 511145.b3743; -.
DR   jPOST; P0ACI6; -.
DR   PaxDb; P0ACI6; -.
DR   PRIDE; P0ACI6; -.
DR   EnsemblBacteria; AAC76766; AAC76766; b3743.
DR   EnsemblBacteria; BAE77545; BAE77545; BAE77545.
DR   GeneID; 67417730; -.
DR   GeneID; 948259; -.
DR   KEGG; ecj:JW3721; -.
DR   KEGG; eco:b3743; -.
DR   PATRIC; fig|1411691.4.peg.2957; -.
DR   EchoBASE; EB0091; -.
DR   eggNOG; COG1522; Bacteria.
DR   HOGENOM; CLU_091233_5_0_6; -.
DR   InParanoid; P0ACI6; -.
DR   OMA; EDCWFIA; -.
DR   PhylomeDB; P0ACI6; -.
DR   BioCyc; EcoCyc:PD00250; -.
DR   EvolutionaryTrace; P0ACI6; -.
DR   PRO; PR:P0ACI6; -.
DR   Proteomes; UP000000318; Chromosome.
DR   Proteomes; UP000000625; Chromosome.
DR   GO; GO:0003700; F:DNA-binding transcription factor activity; IDA:EcoCyc.
DR   GO; GO:0043565; F:sequence-specific DNA binding; IEA:InterPro.
DR   GO; GO:0045892; P:negative regulation of transcription, DNA-templated; IDA:EcoCyc.
DR   GO; GO:0045893; P:positive regulation of transcription, DNA-templated; IDA:EcoCyc.
DR   GO; GO:0043200; P:response to amino acid; IDA:EcoCyc.
DR   CDD; cd00090; HTH_ARSR; 1.
DR   Gene3D; 1.10.10.10; -; 1.
DR   InterPro; IPR011991; ArsR-like_HTH.
DR   InterPro; IPR000485; AsnC-type_HTH_dom.
DR   InterPro; IPR011008; Dimeric_a/b-barrel.
DR   InterPro; IPR019888; Tscrpt_reg_AsnC-like.
DR   InterPro; IPR019887; Tscrpt_reg_AsnC/Lrp_C.
DR   InterPro; IPR019885; Tscrpt_reg_HTH_AsnC-type_CS.
DR   InterPro; IPR036388; WH-like_DNA-bd_sf.
DR   InterPro; IPR036390; WH_DNA-bd_sf.
DR   Pfam; PF01037; AsnC_trans_reg; 1.
DR   PRINTS; PR00033; HTHASNC.
DR   SMART; SM00344; HTH_ASNC; 1.
DR   SUPFAM; SSF46785; SSF46785; 1.
DR   SUPFAM; SSF54909; SSF54909; 1.
DR   PROSITE; PS00519; HTH_ASNC_1; 1.
DR   PROSITE; PS50956; HTH_ASNC_2; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Activator; DNA-binding; Reference proteome; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..152
FT                   /note="Regulatory protein AsnC"
FT                   /id="PRO_0000111719"
FT   DOMAIN          6..67
FT                   /note="HTH asnC-type"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00319"
FT   DNA_BIND        25..44
FT                   /note="H-T-H motif"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00319"
FT   HELIX           8..19
FT                   /evidence="ECO:0007829|PDB:2CG4"
FT   HELIX           25..32
FT                   /evidence="ECO:0007829|PDB:2CG4"
FT   HELIX           36..48
FT                   /evidence="ECO:0007829|PDB:2CG4"
FT   STRAND          51..59
FT                   /evidence="ECO:0007829|PDB:2CG4"
FT   TURN            61..65
FT                   /evidence="ECO:0007829|PDB:2CG4"
FT   STRAND          68..78
FT                   /evidence="ECO:0007829|PDB:2CG4"
FT   HELIX           79..81
FT                   /evidence="ECO:0007829|PDB:2CG4"
FT   HELIX           82..90
FT                   /evidence="ECO:0007829|PDB:2CG4"
FT   STRAND          95..104
FT                   /evidence="ECO:0007829|PDB:2CG4"
FT   STRAND          106..114
FT                   /evidence="ECO:0007829|PDB:2CG4"
FT   HELIX           116..125
FT                   /evidence="ECO:0007829|PDB:2CG4"
FT   TURN            126..129
FT                   /evidence="ECO:0007829|PDB:2CG4"
FT   STRAND          133..140
FT                   /evidence="ECO:0007829|PDB:2CG4"
FT   STRAND          142..147
FT                   /evidence="ECO:0007829|PDB:2CG4"
SQ   SEQUENCE   152 AA;  16888 MW;  B37581D88D15ECED CRC64;
     MENYLIDNLD RGILEALMGN ARTAYAELAK QFGVSPGTIH VRVEKMKQAG IITGARIDVS
     PKQLGYDVGC FIGIILKSAK DYPSALAKLE SLDEVTEAYY TTGHYSIFIK VMCRSIDALQ
     HVLINKIQTI DEIQSTETLI VLQNPIMRTI KP
 
 
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