ID   PAP1_ORFN2              Reviewed;         472 AA.
AC   Q2F8F7;
DT   23-OCT-2007, integrated into UniProtKB/Swiss-Prot.
DT   21-MAR-2006, sequence version 1.
DT   29-SEP-2021, entry version 41.
DE   RecName: Full=Poly(A) polymerase catalytic subunit;
DE            EC=2.7.7.19;
DE   AltName: Full=Poly(A) polymerase large subunit;
DE            Short=PAP-L;
GN   Name=PAPL;
OS   Orf virus (strain NZ2) (OV NZ-2).
OC   Viruses; Varidnaviria; Bamfordvirae; Nucleocytoviricota; Pokkesviricetes;
OC   Chitovirales; Poxviridae; Chordopoxvirinae; Parapoxvirus.
OX   NCBI_TaxID=10259;
OH   NCBI_TaxID=9925; Capra hircus (Goat).
OH   NCBI_TaxID=9606; Homo sapiens (Human).
OH   NCBI_TaxID=9940; Ovis aries (Sheep).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=16274827; DOI=10.1016/j.virusres.2005.09.011;
RA   Mercer A.A., Ueda N., Friederichs S.M., Hofmann K., Fraser K.M.,
RA   Bateman T., Fleming S.B.;
RT   "Comparative analysis of genome sequences of three isolates of Orf virus
RT   reveals unexpected sequence variation.";
RL   Virus Res. 116:146-158(2006).
CC   -!- FUNCTION: Polymerase that creates the 3'-poly(A) tail of mRNA's.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + RNA(n) = diphosphate + RNA(n)-3'-adenine ribonucleotide;
CC         Xref=Rhea:RHEA:11332, Rhea:RHEA-COMP:14527, Rhea:RHEA-COMP:17347,
CC         ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:140395,
CC         ChEBI:CHEBI:173115; EC=2.7.7.19;
CC   -!- SUBUNIT: Heterodimer of a large (catalytic) subunit and a small
CC       (regulatory) subunit. {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the poxviridae poly(A) polymerase catalytic
CC       subunit family. {ECO:0000305}.
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DR   EMBL; DQ184476; ABA00535.1; -; Genomic_DNA.
DR   SMR; Q2F8F7; -.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0004652; F:polynucleotide adenylyltransferase activity; IEA:UniProtKB-EC.
DR   GO; GO:0006397; P:mRNA processing; IEA:UniProtKB-KW.
DR   Gene3D; 1.20.1270.320; -; 1.
DR   Gene3D; 3.30.460.60; -; 1.
DR   InterPro; IPR037265; PolyA_pol_cat_sf.
DR   InterPro; IPR024231; Pox_polyA_pol_nucTrfase.
DR   InterPro; IPR038419; Pox_polyA_pol_nucTrfase_sf.
DR   InterPro; IPR004976; Poxviridae_polyA_pol_cat.
DR   InterPro; IPR024397; Poxvirus_polyA_pol_cat_C.
DR   InterPro; IPR024398; Poxvirus_polyA_pol_cat_N.
DR   InterPro; IPR038337; Poxvirus_polyA_pol_cat_N_sf.
DR   Pfam; PF03296; Pox_polyA_pol; 1.
DR   Pfam; PF12629; Pox_polyA_pol_C; 1.
DR   Pfam; PF12630; Pox_polyA_pol_N; 1.
DR   PIRSF; PIRSF015693; VAC-48L_nuct; 1.
DR   SUPFAM; SSF160957; SSF160957; 1.
PE   3: Inferred from homology;
KW   ATP-binding; mRNA processing; Nucleotide-binding; Transcription;
KW   Transferase.
FT   CHAIN           1..472
FT                   /note="Poly(A) polymerase catalytic subunit"
FT                   /id="PRO_0000308937"
FT   ACT_SITE        191
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        193
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   472 AA;  53032 MW;  FBE20290D08336F9 CRC64;
     MAPPVIEEYL GSRPSMERCV LLRAQRRNMT RVLNFDRKLF LSLVVKSRRR FFKKLGGSEK
     EIAARIEEYF SRQRRLEKLG QILTVLELQS VIVSEFTRTL GSLTTPTPAI NAAVRRVDAP
     RARALASRAL NSYAVLPPPE EAAPMARHKH SDLVEVVKRL VKEHLRRHNK RCVCYGSYAL
     HLLNPEIEYG DIDMVQTNAR PFLINLAFLI YFVTGRQTVL LRVPYLKNYV VLQDEEGGHI
     LDSFNVRQAT LRALPTLLVD NIYVLHPFVQ LMAMLKMFSQ TDRIRDLADN FDKARARMET
     LLAFALEELG EAPADGRTPL PCAFLPPEGG ASRVLEADAR ALDCGFARAV VFLDEPALVQ
     TLLDMGVQDD EIVDFESVSN SAFLVRDNTL FTYFSNTVLM DGDAVHDLSR RGVSAHIVMF
     LLLTRHPAAE GAVRSMLGSL VSDARPVTSV VERERKTGTH GVIDIAKNVI TH