PAP1_VACCW
ID PAP1_VACCW Reviewed; 479 AA.
AC P23371; Q80HX2;
DT 01-NOV-1991, integrated into UniProtKB/Swiss-Prot.
DT 07-DEC-2004, sequence version 2.
DT 29-SEP-2021, entry version 98.
DE RecName: Full=Poly(A) polymerase catalytic subunit;
DE EC=2.7.7.19;
DE AltName: Full=Poly(A) polymerase large subunit;
DE Short=PAP-L;
DE AltName: Full=VP55;
GN Name=PAPL; OrderedLocusNames=VACWR057; ORFNames=E1L;
OS Vaccinia virus (strain Western Reserve) (VACV) (Vaccinia virus (strain
OS WR)).
OC Viruses; Varidnaviria; Bamfordvirae; Nucleocytoviricota; Pokkesviricetes;
OC Chitovirales; Poxviridae; Chordopoxvirinae; Orthopoxvirus; Vaccinia virus.
OX NCBI_TaxID=10254;
OH NCBI_TaxID=9913; Bos taurus (Bovine).
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RA Gershon P.D., Jones E.V., Moss B., Ahn B.-Y.;
RL Submitted (JUL-1990) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Esposito J.J., Frace A.M., Sammons S.A., Olsen-Rasmussen M., Osborne J.,
RA Wohlhueter R.;
RT "Sequencing of the coding region of Vaccinia-WR to an average 9-fold
RT redundancy and an error rate of 0.16/10kb.";
RL Submitted (FEB-2003) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP FUNCTION.
RX PubMed=1670500; DOI=10.1016/0092-8674(91)90048-4;
RA Gershon P.D., Ahn B.-Y., Garfield M., Moss B.;
RT "Poly(A) polymerase and a dissociable polyadenylation stimulatory factor
RT encoded by vaccinia virus.";
RL Cell 66:1269-1278(1991).
CC -!- FUNCTION: Polymerase that creates the 3'-poly(A) tail of mRNA's.
CC {ECO:0000269|PubMed:1670500}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + RNA(n) = diphosphate + RNA(n)-3'-adenine ribonucleotide;
CC Xref=Rhea:RHEA:11332, Rhea:RHEA-COMP:14527, Rhea:RHEA-COMP:17347,
CC ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:140395,
CC ChEBI:CHEBI:173115; EC=2.7.7.19;
CC -!- SUBUNIT: Heterodimer of a large (catalytic) subunit and a small
CC (regulatory) subunit.
CC -!- SIMILARITY: Belongs to the poxviridae poly(A) polymerase catalytic
CC subunit family. {ECO:0000305}.
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DR EMBL; M36339; AAB59821.1; -; Genomic_DNA.
DR EMBL; AY243312; AAO89336.1; -; Genomic_DNA.
DR RefSeq; YP_232939.1; NC_006998.1.
DR PDB; 2GA9; X-ray; 2.30 A; D=11-479.
DR PDB; 2GAF; X-ray; 2.40 A; D=11-479.
DR PDB; 3ER8; X-ray; 3.18 A; C/D=1-479.
DR PDB; 3ER9; X-ray; 2.06 A; B=1-479.
DR PDB; 3ERC; X-ray; 3.21 A; C/D=1-479.
DR PDB; 3OWG; X-ray; 2.86 A; A/B=1-479.
DR PDBsum; 2GA9; -.
DR PDBsum; 2GAF; -.
DR PDBsum; 3ER8; -.
DR PDBsum; 3ER9; -.
DR PDBsum; 3ERC; -.
DR PDBsum; 3OWG; -.
DR SMR; P23371; -.
DR DIP; DIP-48309N; -.
DR IntAct; P23371; 1.
DR DNASU; 3707514; -.
DR GeneID; 3707514; -.
DR KEGG; vg:3707514; -.
DR EvolutionaryTrace; P23371; -.
DR Proteomes; UP000000344; Genome.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0004652; F:polynucleotide adenylyltransferase activity; IEA:UniProtKB-EC.
DR GO; GO:0006397; P:mRNA processing; IEA:UniProtKB-KW.
DR Gene3D; 1.20.1270.320; -; 1.
DR Gene3D; 3.30.460.60; -; 1.
DR InterPro; IPR037265; PolyA_pol_cat_sf.
DR InterPro; IPR024231; Pox_polyA_pol_nucTrfase.
DR InterPro; IPR038419; Pox_polyA_pol_nucTrfase_sf.
DR InterPro; IPR004976; Poxviridae_polyA_pol_cat.
DR InterPro; IPR024397; Poxvirus_polyA_pol_cat_C.
DR InterPro; IPR024398; Poxvirus_polyA_pol_cat_N.
DR InterPro; IPR038337; Poxvirus_polyA_pol_cat_N_sf.
DR Pfam; PF03296; Pox_polyA_pol; 1.
DR Pfam; PF12629; Pox_polyA_pol_C; 1.
DR Pfam; PF12630; Pox_polyA_pol_N; 1.
DR PIRSF; PIRSF015693; VAC-48L_nuct; 1.
DR SUPFAM; SSF160957; SSF160957; 1.
PE 1: Evidence at protein level;
KW 3D-structure; ATP-binding; mRNA processing; Nucleotide-binding;
KW Reference proteome; Transcription; Transferase.
FT CHAIN 1..479
FT /note="Poly(A) polymerase catalytic subunit"
FT /id="PRO_0000099107"
FT ACT_SITE 202
FT /evidence="ECO:0000250"
FT ACT_SITE 204
FT /evidence="ECO:0000250"
FT CONFLICT 10
FT /note="L -> F (in Ref. 1; AAB59821)"
FT /evidence="ECO:0000305"
FT CONFLICT 68
FT /note="N -> D (in Ref. 1; AAB59821)"
FT /evidence="ECO:0000305"
FT CONFLICT 199
FT /note="R -> L (in Ref. 1; AAB59821)"
FT /evidence="ECO:0000305"
FT HELIX 13..22
FT /evidence="ECO:0007829|PDB:3ER9"
FT HELIX 28..34
FT /evidence="ECO:0007829|PDB:3ER9"
FT HELIX 35..37
FT /evidence="ECO:0007829|PDB:3ER9"
FT HELIX 38..46
FT /evidence="ECO:0007829|PDB:3ER9"
FT HELIX 49..63
FT /evidence="ECO:0007829|PDB:3ER9"
FT HELIX 71..81
FT /evidence="ECO:0007829|PDB:3ER9"
FT HELIX 82..87
FT /evidence="ECO:0007829|PDB:3ER9"
FT HELIX 91..111
FT /evidence="ECO:0007829|PDB:3ER9"
FT HELIX 113..116
FT /evidence="ECO:0007829|PDB:3ER9"
FT HELIX 131..134
FT /evidence="ECO:0007829|PDB:3ER9"
FT HELIX 135..145
FT /evidence="ECO:0007829|PDB:3ER9"
FT HELIX 165..178
FT /evidence="ECO:0007829|PDB:3ER9"
FT TURN 180..182
FT /evidence="ECO:0007829|PDB:3ER9"
FT STRAND 183..187
FT /evidence="ECO:0007829|PDB:3ER9"
FT HELIX 188..192
FT /evidence="ECO:0007829|PDB:3ER9"
FT STRAND 204..209
FT /evidence="ECO:0007829|PDB:3ER9"
FT HELIX 210..225
FT /evidence="ECO:0007829|PDB:3ER9"
FT STRAND 230..233
FT /evidence="ECO:0007829|PDB:3ER9"
FT STRAND 237..244
FT /evidence="ECO:0007829|PDB:3ER9"
FT STRAND 250..256
FT /evidence="ECO:0007829|PDB:3ER9"
FT HELIX 259..264
FT /evidence="ECO:0007829|PDB:3ER9"
FT STRAND 267..270
FT /evidence="ECO:0007829|PDB:3ER9"
FT STRAND 273..276
FT /evidence="ECO:0007829|PDB:3ER9"
FT HELIX 278..288
FT /evidence="ECO:0007829|PDB:3ER9"
FT HELIX 292..300
FT /evidence="ECO:0007829|PDB:3ER9"
FT HELIX 302..320
FT /evidence="ECO:0007829|PDB:3ER9"
FT STRAND 335..338
FT /evidence="ECO:0007829|PDB:3ER9"
FT TURN 339..342
FT /evidence="ECO:0007829|PDB:3ER9"
FT STRAND 343..347
FT /evidence="ECO:0007829|PDB:3ER9"
FT TURN 349..351
FT /evidence="ECO:0007829|PDB:3ER9"
FT STRAND 352..361
FT /evidence="ECO:0007829|PDB:3ER9"
FT HELIX 363..372
FT /evidence="ECO:0007829|PDB:3ER9"
FT HELIX 382..384
FT /evidence="ECO:0007829|PDB:3ER9"
FT STRAND 386..392
FT /evidence="ECO:0007829|PDB:3ER9"
FT STRAND 395..399
FT /evidence="ECO:0007829|PDB:3ER9"
FT STRAND 405..408
FT /evidence="ECO:0007829|PDB:3ER9"
FT HELIX 414..430
FT /evidence="ECO:0007829|PDB:3ER9"
FT HELIX 436..444
FT /evidence="ECO:0007829|PDB:3ER9"
FT STRAND 468..471
FT /evidence="ECO:0007829|PDB:3ER9"
FT TURN 472..475
FT /evidence="ECO:0007829|PDB:3ER9"
FT STRAND 476..478
FT /evidence="ECO:0007829|PDB:3ER9"
SQ SEQUENCE 479 AA; 55531 MW; 2E76F2BB1A246D5F CRC64;
MNRNPDQNTL PNITLKIIET YLGRVPSVNE YHMLKLQARN IQKITVFNKD IFVSLVKKNK
KRFFSDVNTS ASEIKDRILS YFSKQTQTYN IGKLFTIIEL QSVLVTTYTD ILGVLTIKAP
NVISSKISYN VTSMEELARD MLNSMNVAVI DKAKVMGRHN VSSLVKNVNK LMEEYLRRHN
KSCICYGSYS LYLINPNIRY GDIDILQTNS RTFLIDLAFL IKFITGNNII LSKIPYLRNY
MVIKDENDNH IIDSFNIRQD TMNVVPKIFI DNIYIVDPTF QLLNMIKMFS QIDRLEDLSK
DPEKFNARMA TMLEYVRYTH GIVFDGKRNN MPMKCIIDEN NRIVTVTTKD YFSFKKCLVY
LDENVLSSDI LDLNADTSCD FESVTNSVYL IHDNIMYTYF SNTILLSDKG KVHEISARGL
CAHILLYQML TSGEYKQCLS DLLNSMMNRD KIPIYSHTER DKKPGRHGFI NIEKDIIVF