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PAP1_VACCW
ID   PAP1_VACCW              Reviewed;         479 AA.
AC   P23371; Q80HX2;
DT   01-NOV-1991, integrated into UniProtKB/Swiss-Prot.
DT   07-DEC-2004, sequence version 2.
DT   29-SEP-2021, entry version 98.
DE   RecName: Full=Poly(A) polymerase catalytic subunit;
DE            EC=2.7.7.19;
DE   AltName: Full=Poly(A) polymerase large subunit;
DE            Short=PAP-L;
DE   AltName: Full=VP55;
GN   Name=PAPL; OrderedLocusNames=VACWR057; ORFNames=E1L;
OS   Vaccinia virus (strain Western Reserve) (VACV) (Vaccinia virus (strain
OS   WR)).
OC   Viruses; Varidnaviria; Bamfordvirae; Nucleocytoviricota; Pokkesviricetes;
OC   Chitovirales; Poxviridae; Chordopoxvirinae; Orthopoxvirus; Vaccinia virus.
OX   NCBI_TaxID=10254;
OH   NCBI_TaxID=9913; Bos taurus (Bovine).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RA   Gershon P.D., Jones E.V., Moss B., Ahn B.-Y.;
RL   Submitted (JUL-1990) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA   Esposito J.J., Frace A.M., Sammons S.A., Olsen-Rasmussen M., Osborne J.,
RA   Wohlhueter R.;
RT   "Sequencing of the coding region of Vaccinia-WR to an average 9-fold
RT   redundancy and an error rate of 0.16/10kb.";
RL   Submitted (FEB-2003) to the EMBL/GenBank/DDBJ databases.
RN   [3]
RP   FUNCTION.
RX   PubMed=1670500; DOI=10.1016/0092-8674(91)90048-4;
RA   Gershon P.D., Ahn B.-Y., Garfield M., Moss B.;
RT   "Poly(A) polymerase and a dissociable polyadenylation stimulatory factor
RT   encoded by vaccinia virus.";
RL   Cell 66:1269-1278(1991).
CC   -!- FUNCTION: Polymerase that creates the 3'-poly(A) tail of mRNA's.
CC       {ECO:0000269|PubMed:1670500}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + RNA(n) = diphosphate + RNA(n)-3'-adenine ribonucleotide;
CC         Xref=Rhea:RHEA:11332, Rhea:RHEA-COMP:14527, Rhea:RHEA-COMP:17347,
CC         ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:140395,
CC         ChEBI:CHEBI:173115; EC=2.7.7.19;
CC   -!- SUBUNIT: Heterodimer of a large (catalytic) subunit and a small
CC       (regulatory) subunit.
CC   -!- SIMILARITY: Belongs to the poxviridae poly(A) polymerase catalytic
CC       subunit family. {ECO:0000305}.
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DR   EMBL; M36339; AAB59821.1; -; Genomic_DNA.
DR   EMBL; AY243312; AAO89336.1; -; Genomic_DNA.
DR   RefSeq; YP_232939.1; NC_006998.1.
DR   PDB; 2GA9; X-ray; 2.30 A; D=11-479.
DR   PDB; 2GAF; X-ray; 2.40 A; D=11-479.
DR   PDB; 3ER8; X-ray; 3.18 A; C/D=1-479.
DR   PDB; 3ER9; X-ray; 2.06 A; B=1-479.
DR   PDB; 3ERC; X-ray; 3.21 A; C/D=1-479.
DR   PDB; 3OWG; X-ray; 2.86 A; A/B=1-479.
DR   PDBsum; 2GA9; -.
DR   PDBsum; 2GAF; -.
DR   PDBsum; 3ER8; -.
DR   PDBsum; 3ER9; -.
DR   PDBsum; 3ERC; -.
DR   PDBsum; 3OWG; -.
DR   SMR; P23371; -.
DR   DIP; DIP-48309N; -.
DR   IntAct; P23371; 1.
DR   DNASU; 3707514; -.
DR   GeneID; 3707514; -.
DR   KEGG; vg:3707514; -.
DR   EvolutionaryTrace; P23371; -.
DR   Proteomes; UP000000344; Genome.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0004652; F:polynucleotide adenylyltransferase activity; IEA:UniProtKB-EC.
DR   GO; GO:0006397; P:mRNA processing; IEA:UniProtKB-KW.
DR   Gene3D; 1.20.1270.320; -; 1.
DR   Gene3D; 3.30.460.60; -; 1.
DR   InterPro; IPR037265; PolyA_pol_cat_sf.
DR   InterPro; IPR024231; Pox_polyA_pol_nucTrfase.
DR   InterPro; IPR038419; Pox_polyA_pol_nucTrfase_sf.
DR   InterPro; IPR004976; Poxviridae_polyA_pol_cat.
DR   InterPro; IPR024397; Poxvirus_polyA_pol_cat_C.
DR   InterPro; IPR024398; Poxvirus_polyA_pol_cat_N.
DR   InterPro; IPR038337; Poxvirus_polyA_pol_cat_N_sf.
DR   Pfam; PF03296; Pox_polyA_pol; 1.
DR   Pfam; PF12629; Pox_polyA_pol_C; 1.
DR   Pfam; PF12630; Pox_polyA_pol_N; 1.
DR   PIRSF; PIRSF015693; VAC-48L_nuct; 1.
DR   SUPFAM; SSF160957; SSF160957; 1.
PE   1: Evidence at protein level;
KW   3D-structure; ATP-binding; mRNA processing; Nucleotide-binding;
KW   Reference proteome; Transcription; Transferase.
FT   CHAIN           1..479
FT                   /note="Poly(A) polymerase catalytic subunit"
FT                   /id="PRO_0000099107"
FT   ACT_SITE        202
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        204
FT                   /evidence="ECO:0000250"
FT   CONFLICT        10
FT                   /note="L -> F (in Ref. 1; AAB59821)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        68
FT                   /note="N -> D (in Ref. 1; AAB59821)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        199
FT                   /note="R -> L (in Ref. 1; AAB59821)"
FT                   /evidence="ECO:0000305"
FT   HELIX           13..22
FT                   /evidence="ECO:0007829|PDB:3ER9"
FT   HELIX           28..34
FT                   /evidence="ECO:0007829|PDB:3ER9"
FT   HELIX           35..37
FT                   /evidence="ECO:0007829|PDB:3ER9"
FT   HELIX           38..46
FT                   /evidence="ECO:0007829|PDB:3ER9"
FT   HELIX           49..63
FT                   /evidence="ECO:0007829|PDB:3ER9"
FT   HELIX           71..81
FT                   /evidence="ECO:0007829|PDB:3ER9"
FT   HELIX           82..87
FT                   /evidence="ECO:0007829|PDB:3ER9"
FT   HELIX           91..111
FT                   /evidence="ECO:0007829|PDB:3ER9"
FT   HELIX           113..116
FT                   /evidence="ECO:0007829|PDB:3ER9"
FT   HELIX           131..134
FT                   /evidence="ECO:0007829|PDB:3ER9"
FT   HELIX           135..145
FT                   /evidence="ECO:0007829|PDB:3ER9"
FT   HELIX           165..178
FT                   /evidence="ECO:0007829|PDB:3ER9"
FT   TURN            180..182
FT                   /evidence="ECO:0007829|PDB:3ER9"
FT   STRAND          183..187
FT                   /evidence="ECO:0007829|PDB:3ER9"
FT   HELIX           188..192
FT                   /evidence="ECO:0007829|PDB:3ER9"
FT   STRAND          204..209
FT                   /evidence="ECO:0007829|PDB:3ER9"
FT   HELIX           210..225
FT                   /evidence="ECO:0007829|PDB:3ER9"
FT   STRAND          230..233
FT                   /evidence="ECO:0007829|PDB:3ER9"
FT   STRAND          237..244
FT                   /evidence="ECO:0007829|PDB:3ER9"
FT   STRAND          250..256
FT                   /evidence="ECO:0007829|PDB:3ER9"
FT   HELIX           259..264
FT                   /evidence="ECO:0007829|PDB:3ER9"
FT   STRAND          267..270
FT                   /evidence="ECO:0007829|PDB:3ER9"
FT   STRAND          273..276
FT                   /evidence="ECO:0007829|PDB:3ER9"
FT   HELIX           278..288
FT                   /evidence="ECO:0007829|PDB:3ER9"
FT   HELIX           292..300
FT                   /evidence="ECO:0007829|PDB:3ER9"
FT   HELIX           302..320
FT                   /evidence="ECO:0007829|PDB:3ER9"
FT   STRAND          335..338
FT                   /evidence="ECO:0007829|PDB:3ER9"
FT   TURN            339..342
FT                   /evidence="ECO:0007829|PDB:3ER9"
FT   STRAND          343..347
FT                   /evidence="ECO:0007829|PDB:3ER9"
FT   TURN            349..351
FT                   /evidence="ECO:0007829|PDB:3ER9"
FT   STRAND          352..361
FT                   /evidence="ECO:0007829|PDB:3ER9"
FT   HELIX           363..372
FT                   /evidence="ECO:0007829|PDB:3ER9"
FT   HELIX           382..384
FT                   /evidence="ECO:0007829|PDB:3ER9"
FT   STRAND          386..392
FT                   /evidence="ECO:0007829|PDB:3ER9"
FT   STRAND          395..399
FT                   /evidence="ECO:0007829|PDB:3ER9"
FT   STRAND          405..408
FT                   /evidence="ECO:0007829|PDB:3ER9"
FT   HELIX           414..430
FT                   /evidence="ECO:0007829|PDB:3ER9"
FT   HELIX           436..444
FT                   /evidence="ECO:0007829|PDB:3ER9"
FT   STRAND          468..471
FT                   /evidence="ECO:0007829|PDB:3ER9"
FT   TURN            472..475
FT                   /evidence="ECO:0007829|PDB:3ER9"
FT   STRAND          476..478
FT                   /evidence="ECO:0007829|PDB:3ER9"
SQ   SEQUENCE   479 AA;  55531 MW;  2E76F2BB1A246D5F CRC64;
     MNRNPDQNTL PNITLKIIET YLGRVPSVNE YHMLKLQARN IQKITVFNKD IFVSLVKKNK
     KRFFSDVNTS ASEIKDRILS YFSKQTQTYN IGKLFTIIEL QSVLVTTYTD ILGVLTIKAP
     NVISSKISYN VTSMEELARD MLNSMNVAVI DKAKVMGRHN VSSLVKNVNK LMEEYLRRHN
     KSCICYGSYS LYLINPNIRY GDIDILQTNS RTFLIDLAFL IKFITGNNII LSKIPYLRNY
     MVIKDENDNH IIDSFNIRQD TMNVVPKIFI DNIYIVDPTF QLLNMIKMFS QIDRLEDLSK
     DPEKFNARMA TMLEYVRYTH GIVFDGKRNN MPMKCIIDEN NRIVTVTTKD YFSFKKCLVY
     LDENVLSSDI LDLNADTSCD FESVTNSVYL IHDNIMYTYF SNTILLSDKG KVHEISARGL
     CAHILLYQML TSGEYKQCLS DLLNSMMNRD KIPIYSHTER DKKPGRHGFI NIEKDIIVF
 
 
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