PAP6_ARATH
ID PAP6_ARATH Reviewed; 284 AA.
AC Q9LW57; Q8LBY7;
DT 15-MAY-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 25-MAY-2022, entry version 114.
DE RecName: Full=Plastid-lipid-associated protein 6, chloroplastic {ECO:0000303|PubMed:16850419};
DE AltName: Full=Fibrillin-4 {ECO:0000303|PubMed:16461379};
DE AltName: Full=Fibrillin-6 {ECO:0000303|PubMed:18455927};
DE AltName: Full=Harpin-binding protein 1 {ECO:0000303|Ref.1};
DE Short=HrBP1 {ECO:0000303|Ref.1};
DE AltName: Full=Plastoglobulin 30.4 {ECO:0000303|PubMed:16414959};
DE Short=AtPGL30.4 {ECO:0000303|PubMed:16414959};
DE Flags: Precursor;
GN Name=PAP6 {ECO:0000303|PubMed:16850419};
GN Synonyms=FBN4 {ECO:0000303|PubMed:21571574},
GN FIB4 {ECO:0000303|PubMed:16461379}, HRBP1 {ECO:0000303|Ref.1},
GN PGL30.4 {ECO:0000303|PubMed:16414959};
GN OrderedLocusNames=At3g23400 {ECO:0000312|Araport:AT3G23400};
GN ORFNames=MLM24.13 {ECO:0000312|EMBL:BAB02284.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RA Song X., Linderoth N.A., Bariola P.A., Bednarski D., Fan H., Wei Z.;
RT "The Arabidopsis HrBP1 gene encodes a harpin-interacting factor that is a
RT member of a PAP/fibrillin subfamily.";
RL Submitted (SEP-2003) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10819329; DOI=10.1093/dnares/7.2.131;
RA Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 3. I. Sequence
RT features of the regions of 4,504,864 bp covered by sixty P1 and TAC
RT clones.";
RL DNA Res. 7:131-135(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA Feldmann K.A.;
RT "Full-length cDNA from Arabidopsis thaliana.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Shinn P., Chen H., Kim C.J., Ecker J.R.;
RT "Arabidopsis ORF clones.";
RL Submitted (MAR-2006) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP SUBCELLULAR LOCATION.
RC STRAIN=cv. Col-2;
RX PubMed=16414959; DOI=10.1074/jbc.m511939200;
RA Vidi P.-A., Kanwischer M., Baginsky S., Austin J.R., Csucs G., Doermann P.,
RA Kessler F., Brehelin C.;
RT "Tocopherol cyclase (VTE1) localization and vitamin E accumulation in
RT chloroplast plastoglobule lipoprotein particles.";
RL J. Biol. Chem. 281:11225-11234(2006).
RN [7]
RP IDENTIFICATION BY MASS SPECTROMETRY, AND SUBCELLULAR LOCATION [LARGE SCALE
RP ANALYSIS].
RC STRAIN=cv. Columbia;
RX PubMed=16461379; DOI=10.1104/pp.105.076083;
RA Ytterberg A.J., Peltier J.-B., van Wijk K.J.;
RT "Protein profiling of plastoglobules in chloroplasts and chromoplasts. A
RT surprising site for differential accumulation of metabolic enzymes.";
RL Plant Physiol. 140:984-997(2006).
RN [8]
RP PHOSPHORYLATION.
RC STRAIN=cv. Col-5;
RX PubMed=16850419; DOI=10.1002/pmic.200500172;
RA Jones A.M., Bennett M.H., Mansfield J.W., Grant M.;
RT "Analysis of the defence phosphoproteome of Arabidopsis thaliana using
RT differential mass tagging.";
RL Proteomics 6:4155-4165(2006).
RN [9]
RP IDENTIFICATION IN THE LHCII COMPLEX, AND IDENTIFICATION BY MASS
RP SPECTROMETRY.
RX PubMed=18455927; DOI=10.1016/j.jasms.2008.03.014;
RA Galetskiy D., Susnea I., Reiser V., Adamska I., Przybylski M.;
RT "Structure and dynamics of photosystem II light-harvesting complex revealed
RT by high-resolution FTICR mass spectrometric proteome analysis.";
RL J. Am. Soc. Mass Spectrom. 19:1004-1013(2008).
RN [10]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-105, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=19376835; DOI=10.1104/pp.109.138677;
RA Reiland S., Messerli G., Baerenfaller K., Gerrits B., Endler A.,
RA Grossmann J., Gruissem W., Baginsky S.;
RT "Large-scale Arabidopsis phosphoproteome profiling reveals novel
RT chloroplast kinase substrates and phosphorylation networks.";
RL Plant Physiol. 150:889-903(2009).
RN [11]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=20813909; DOI=10.1104/pp.110.164095;
RA Singh D.K., Maximova S.N., Jensen P.J., Lehman B.L., Ngugi H.K.,
RA McNellis T.W.;
RT "FIBRILLIN4 is required for plastoglobule development and stress resistance
RT in apple and Arabidopsis.";
RL Plant Physiol. 154:1281-1293(2010).
RN [12]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=21571574; DOI=10.1016/j.tplants.2011.03.014;
RA Singh D.K., McNellis T.W.;
RT "Fibrillin protein function: the tip of the iceberg?";
RL Trends Plant Sci. 16:432-441(2011).
RN [13]
RP IDENTIFICATION BY MASS SPECTROMETRY, AND SUBCELLULAR LOCATION [LARGE SCALE
RP ANALYSIS].
RC STRAIN=cv. Columbia;
RX PubMed=22274653; DOI=10.1104/pp.111.193144;
RA Lundquist P.K., Poliakov A., Bhuiyan N.H., Zybailov B., Sun Q.,
RA van Wijk K.J.;
RT "The functional network of the Arabidopsis plastoglobule proteome based on
RT quantitative proteomics and genome-wide coexpression analysis.";
RL Plant Physiol. 158:1172-1192(2012).
RN [14]
RP 3D-STRUCTURE MODELING, AND PHOSPHORYLATION AT SER-96; SER-105; SER-148;
RP SER-151 AND SER-155.
RX PubMed=26962209; DOI=10.1093/jxb/erw091;
RA Lohscheider J.N., Friso G., van Wijk K.J.;
RT "Phosphorylation of plastoglobular proteins in Arabidopsis thaliana.";
RL J. Exp. Bot. 67:3975-3984(2016).
CC -!- FUNCTION: Required for plastoglobule development and resistance to
CC multiple stresses. Regulates plastoglobule osmiophilic content. May be
CC involved in the transport of lipophilic antioxidants in and out of the
CC plastoglobule. {ECO:0000269|PubMed:20813909}.
CC -!- SUBUNIT: Part of the Photosystem II light-harvesting complex (LHCII).
CC {ECO:0000269|PubMed:18455927}.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast, plastoglobule
CC {ECO:0000269|PubMed:16414959, ECO:0000269|PubMed:16461379,
CC ECO:0000269|PubMed:22274653}.
CC -!- PTM: Phosphorylated as part of a basal defense response.
CC {ECO:0000269|PubMed:16850419}.
CC -!- DISRUPTION PHENOTYPE: Increased susceptibility to ozone and to
CC bacterial disease. {ECO:0000269|PubMed:20813909}.
CC -!- SIMILARITY: Belongs to the PAP/fibrillin family. {ECO:0000305}.
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DR EMBL; AY383618; AAR26476.1; -; mRNA.
DR EMBL; AB015474; BAB02284.1; -; Genomic_DNA.
DR EMBL; CP002686; AEE76761.1; -; Genomic_DNA.
DR EMBL; AY086921; AAM64485.1; -; mRNA.
DR EMBL; BT024911; ABD91502.1; -; mRNA.
DR RefSeq; NP_566728.1; NM_113243.4.
DR AlphaFoldDB; Q9LW57; -.
DR SMR; Q9LW57; -.
DR BioGRID; 7253; 3.
DR IntAct; Q9LW57; 1.
DR STRING; 3702.AT3G23400.1; -.
DR iPTMnet; Q9LW57; -.
DR PaxDb; Q9LW57; -.
DR PRIDE; Q9LW57; -.
DR ProteomicsDB; 236322; -.
DR EnsemblPlants; AT3G23400.1; AT3G23400.1; AT3G23400.
DR GeneID; 821921; -.
DR Gramene; AT3G23400.1; AT3G23400.1; AT3G23400.
DR KEGG; ath:AT3G23400; -.
DR Araport; AT3G23400; -.
DR TAIR; locus:2090890; AT3G23400.
DR eggNOG; ENOG502QVEU; Eukaryota.
DR HOGENOM; CLU_069245_3_0_1; -.
DR OMA; IPPFDIP; -.
DR OrthoDB; 1220408at2759; -.
DR PhylomeDB; Q9LW57; -.
DR PRO; PR:Q9LW57; -.
DR Proteomes; UP000006548; Chromosome 3.
DR ExpressionAtlas; Q9LW57; baseline and differential.
DR Genevisible; Q9LW57; AT.
DR GO; GO:0009507; C:chloroplast; HDA:TAIR.
DR GO; GO:0009941; C:chloroplast envelope; HDA:TAIR.
DR GO; GO:0009534; C:chloroplast thylakoid; HDA:TAIR.
DR GO; GO:0009535; C:chloroplast thylakoid membrane; HDA:TAIR.
DR GO; GO:0005829; C:cytosol; HDA:TAIR.
DR GO; GO:0005634; C:nucleus; HDA:TAIR.
DR GO; GO:0005886; C:plasma membrane; HDA:TAIR.
DR GO; GO:0010287; C:plastoglobule; HDA:TAIR.
DR GO; GO:0009579; C:thylakoid; HDA:TAIR.
DR GO; GO:0031977; C:thylakoid lumen; HDA:TAIR.
DR GO; GO:0009658; P:chloroplast organization; IMP:TAIR.
DR GO; GO:0042742; P:defense response to bacterium; IMP:TAIR.
DR GO; GO:0010193; P:response to ozone; IMP:TAIR.
DR InterPro; IPR039633; PAP.
DR InterPro; IPR006843; PAP/fibrillin_dom.
DR PANTHER; PTHR31906; PTHR31906; 1.
DR Pfam; PF04755; PAP_fibrillin; 1.
DR PROSITE; PS00213; LIPOCALIN; 1.
PE 1: Evidence at protein level;
KW Chloroplast; Phosphoprotein; Plastid; Reference proteome; Transit peptide.
FT TRANSIT 1..72
FT /note="Chloroplast"
FT /evidence="ECO:0000255"
FT CHAIN 73..284
FT /note="Plastid-lipid-associated protein 6, chloroplastic"
FT /id="PRO_0000286532"
FT REGION 1..47
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 29..43
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 96
FT /note="Phosphoserine"
FT /evidence="ECO:0000305|PubMed:26962209"
FT MOD_RES 105
FT /note="Phosphoserine"
FT /evidence="ECO:0000305|PubMed:26962209,
FT ECO:0007744|PubMed:19376835"
FT MOD_RES 148
FT /note="Phosphoserine"
FT /evidence="ECO:0000305|PubMed:26962209"
FT MOD_RES 151
FT /note="Phosphoserine"
FT /evidence="ECO:0000305|PubMed:26962209"
FT MOD_RES 155
FT /note="Phosphoserine"
FT /evidence="ECO:0000305|PubMed:26962209"
FT CONFLICT 177
FT /note="D -> N (in Ref. 4; AAM64485)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 284 AA; 30455 MW; 142104702C1DC284 CRC64;
MATSSTFSSL LPSPPALLSD HRSPPPSIRY SFSPLTTPKS SRLGFTVPEK RNLAANSSLV
EVSIGGESDP PPSSSGSGGD DKQIALLKLK LLSVVSGLNR GLVASVDDLE RAEVAAKELE
TAGGPVDLTD DLDKLQGKWR LLYSSAFSSR SLGGSRPGLP TGRLIPVTLG QVFQRIDVFS
KDFDNIAEVE LGAPWPFPPL EATATLAHKF ELLGTCKIKI TFEKTTVKTS GNLSQIPPFD
IPRLPDSFRP SSNPGTGDFE VTYVDDTMRI TRGDRGELRV FVIA
