ID   PAPA1_MYCBO             Reviewed;         511 AA.
AC   Q7TVK9; A0A1R3Y5E3; X2BQ32;
DT   15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2003, sequence version 1.
DT   03-AUG-2022, entry version 80.
DE   RecName: Full=2'-acyl-2-O-sulfo-trehalose (hydroxy)phthioceranyltransferase PapA1 {ECO:0000250|UniProtKB:P9WIK9};
DE            EC=2.3.1.283 {ECO:0000250|UniProtKB:P9WIK9};
DE   AltName: Full=Polyketide synthase-associated protein A1;
DE   AltName: Full=SL659 acyltransferase PapA1 {ECO:0000250|UniProtKB:P9WIK9};
GN   Name=papA1; OrderedLocusNames=BQ2027_MB3854C;
OS   Mycobacterium bovis (strain ATCC BAA-935 / AF2122/97).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycobacterium; Mycobacterium tuberculosis complex.
OX   NCBI_TaxID=233413;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC BAA-935 / AF2122/97;
RX   PubMed=12788972; DOI=10.1073/pnas.1130426100;
RA   Garnier T., Eiglmeier K., Camus J.-C., Medina N., Mansoor H., Pryor M.,
RA   Duthoy S., Grondin S., Lacroix C., Monsempe C., Simon S., Harris B.,
RA   Atkin R., Doggett J., Mayes R., Keating L., Wheeler P.R., Parkhill J.,
RA   Barrell B.G., Cole S.T., Gordon S.V., Hewinson R.G.;
RT   "The complete genome sequence of Mycobacterium bovis.";
RL   Proc. Natl. Acad. Sci. U.S.A. 100:7877-7882(2003).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND GENOME REANNOTATION.
RC   STRAIN=ATCC BAA-935 / AF2122/97;
RX   PubMed=28385856; DOI=10.1128/genomea.00157-17;
RA   Malone K.M., Farrell D., Stuber T.P., Schubert O.T., Aebersold R.,
RA   Robbe-Austerman S., Gordon S.V.;
RT   "Updated reference genome sequence and annotation of Mycobacterium bovis
RT   AF2122/97.";
RL   Genome Announc. 5:E00157-E00157(2017).
CC   -!- FUNCTION: Required for the biosynthesis of sulfolipid-1 (SL-1), a major
CC       mycobacterial cell wall lipid. Catalyzes the acylation of trehalose-2-
CC       sulfate-2'-palmitate (SL659) by adding the (hydroxy)phthioceranoyl
CC       group at the 3'-position to yield the diacylated intermediate 2-
CC       palmitoyl-3-(C43)-phthioceranyl-alpha, alpha'-D-trehalose-2'-sulfate
CC       (SL1278). {ECO:0000250|UniProtKB:P9WIK9}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a (hydroxy)phthioceranyl-[(hydroxy)phthioceranic acid
CC         synthase] + 2'-palmitoyl/stearoyl-2-O-sulfo-alpha,alpha-trehalose = a
CC         3'-(hydroxy)phthioceranyl-2'-palmitoyl/stearoyl-2-O-sulfo-
CC         alpha,alpha-trehalose + holo-[(hydroxy)phthioceranic acid synthase].;
CC         EC=2.3.1.283; Evidence={ECO:0000250|UniProtKB:P9WIK9};
CC   -!- SIMILARITY: Belongs to the PapA acyltransferase family. {ECO:0000305}.
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DR   EMBL; LT708304; SIU02483.1; -; Genomic_DNA.
DR   RefSeq; NP_857491.1; NC_002945.3.
DR   RefSeq; WP_003899711.1; NC_002945.4.
DR   AlphaFoldDB; Q7TVK9; -.
DR   SMR; Q7TVK9; -.
DR   EnsemblBacteria; SIU02483; SIU02483; BQ2027_MB3854C.
DR   PATRIC; fig|233413.5.peg.4215; -.
DR   OMA; AEIHLMY; -.
DR   Proteomes; UP000001419; Chromosome.
DR   GO; GO:0016746; F:acyltransferase activity; IEA:UniProtKB-KW.
DR   GO; GO:0071555; P:cell wall organization; IEA:UniProtKB-KW.
DR   GO; GO:0008610; P:lipid biosynthetic process; IEA:UniProt.
DR   Gene3D; 3.30.559.10; -; 1.
DR   InterPro; IPR023213; CAT-like_dom_sf.
DR   InterPro; IPR001242; Condensatn.
DR   Pfam; PF00668; Condensation; 1.
PE   3: Inferred from homology;
KW   Acyltransferase; Cell wall biogenesis/degradation; Lipid biosynthesis;
KW   Lipid metabolism; Transferase.
FT   CHAIN           1..511
FT                   /note="2'-acyl-2-O-sulfo-trehalose
FT                   (hydroxy)phthioceranyltransferase PapA1"
FT                   /id="PRO_0000314656"
SQ   SEQUENCE   511 AA;  56128 MW;  07D7766EF181C4E5 CRC64;
     MRIGPVELSA VKDWDPAPGV LVSWHPTPAS CAKALAAPVS AVPPSYVQAR QIRSFSEQAA
     RGLDHSRLLI ASVEVFGHCD LRAMTYVINA HLRRHDTYRS WFELRDTDHI VRHSIADPAD
     IEFVPTTHGE MTSADLRQHI VATPDSLHWD CFSFGVIQRA DSFTFYASID HLHADGQFVG
     VGLMEFQSMY TALIMGEPPI GLSEAGSYVD FCVRQHEYTS ALTVDSPEVR AWIDFAEINN
     GTFPEFPLPL GDPSVRCGGD LLSMMLMDEQ QTQRFESACM AANARFIGGM LACIAIAIHE
     LTGADTYFGI TPKDIRTPAD LMTQGWFTGQ IPVTVPVAGL SFNEIARIAQ TSFDTGADLA
     KVPFERVVEL SPSLRRPQPL FSLVNFFDAQ VGPLSAVTKL FEGLNVGTYS DGRVTYPLST
     MVGRFDETAA SVLFPDNPVA RESVTAYLRA IRSVCMRIAN GGTAERVGNV VALSPGRRNN
     IERMTWRSCR AGDFIDICNL KVANVTVDRE A