ID   ASND1_DANRE             Reviewed;         612 AA.
AC   Q8JFW1; Q7ZTT7;
DT   18-MAR-2008, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2002, sequence version 1.
DT   03-AUG-2022, entry version 88.
DE   RecName: Full=Asparagine synthetase domain-containing protein 1;
GN   Name=asnsd1; ORFNames=si:dz211o13.6;
OS   Danio rerio (Zebrafish) (Brachydanio rerio).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes;
OC   Danionidae; Danioninae; Danio.
OX   NCBI_TaxID=7955;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Tuebingen;
RX   PubMed=23594743; DOI=10.1038/nature12111;
RA   Howe K., Clark M.D., Torroja C.F., Torrance J., Berthelot C., Muffato M.,
RA   Collins J.E., Humphray S., McLaren K., Matthews L., McLaren S., Sealy I.,
RA   Caccamo M., Churcher C., Scott C., Barrett J.C., Koch R., Rauch G.J.,
RA   White S., Chow W., Kilian B., Quintais L.T., Guerra-Assuncao J.A., Zhou Y.,
RA   Gu Y., Yen J., Vogel J.H., Eyre T., Redmond S., Banerjee R., Chi J., Fu B.,
RA   Langley E., Maguire S.F., Laird G.K., Lloyd D., Kenyon E., Donaldson S.,
RA   Sehra H., Almeida-King J., Loveland J., Trevanion S., Jones M., Quail M.,
RA   Willey D., Hunt A., Burton J., Sims S., McLay K., Plumb B., Davis J.,
RA   Clee C., Oliver K., Clark R., Riddle C., Elliot D., Threadgold G.,
RA   Harden G., Ware D., Begum S., Mortimore B., Kerry G., Heath P.,
RA   Phillimore B., Tracey A., Corby N., Dunn M., Johnson C., Wood J., Clark S.,
RA   Pelan S., Griffiths G., Smith M., Glithero R., Howden P., Barker N.,
RA   Lloyd C., Stevens C., Harley J., Holt K., Panagiotidis G., Lovell J.,
RA   Beasley H., Henderson C., Gordon D., Auger K., Wright D., Collins J.,
RA   Raisen C., Dyer L., Leung K., Robertson L., Ambridge K., Leongamornlert D.,
RA   McGuire S., Gilderthorp R., Griffiths C., Manthravadi D., Nichol S.,
RA   Barker G., Whitehead S., Kay M., Brown J., Murnane C., Gray E.,
RA   Humphries M., Sycamore N., Barker D., Saunders D., Wallis J., Babbage A.,
RA   Hammond S., Mashreghi-Mohammadi M., Barr L., Martin S., Wray P.,
RA   Ellington A., Matthews N., Ellwood M., Woodmansey R., Clark G., Cooper J.,
RA   Tromans A., Grafham D., Skuce C., Pandian R., Andrews R., Harrison E.,
RA   Kimberley A., Garnett J., Fosker N., Hall R., Garner P., Kelly D., Bird C.,
RA   Palmer S., Gehring I., Berger A., Dooley C.M., Ersan-Urun Z., Eser C.,
RA   Geiger H., Geisler M., Karotki L., Kirn A., Konantz J., Konantz M.,
RA   Oberlander M., Rudolph-Geiger S., Teucke M., Lanz C., Raddatz G.,
RA   Osoegawa K., Zhu B., Rapp A., Widaa S., Langford C., Yang F.,
RA   Schuster S.C., Carter N.P., Harrow J., Ning Z., Herrero J., Searle S.M.,
RA   Enright A., Geisler R., Plasterk R.H., Lee C., Westerfield M.,
RA   de Jong P.J., Zon L.I., Postlethwait J.H., Nusslein-Volhard C.,
RA   Hubbard T.J., Roest Crollius H., Rogers J., Stemple D.L.;
RT   "The zebrafish reference genome sequence and its relationship to the human
RT   genome.";
RL   Nature 496:498-503(2013).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RG   NIH - Zebrafish Gene Collection (ZGC) project;
RL   Submitted (APR-2003) to the EMBL/GenBank/DDBJ databases.
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DR   EMBL; AL591593; CAD43473.1; -; Genomic_DNA.
DR   EMBL; BC051619; AAH51619.1; -; mRNA.
DR   RefSeq; NP_956389.1; NM_200095.1.
DR   AlphaFoldDB; Q8JFW1; -.
DR   SMR; Q8JFW1; -.
DR   STRING; 7955.ENSDARP00000123026; -.
DR   PaxDb; Q8JFW1; -.
DR   GeneID; 368827; -.
DR   KEGG; dre:368827; -.
DR   CTD; 54529; -.
DR   ZFIN; ZDB-GENE-030616-362; asnsd1.
DR   eggNOG; KOG0573; Eukaryota.
DR   InParanoid; Q8JFW1; -.
DR   PhylomeDB; Q8JFW1; -.
DR   PRO; PR:Q8JFW1; -.
DR   Proteomes; UP000000437; Genome assembly.
DR   Proteomes; UP000814640; Unplaced.
DR   GO; GO:0004066; F:asparagine synthase (glutamine-hydrolyzing) activity; IEA:InterPro.
DR   GO; GO:0006529; P:asparagine biosynthetic process; IEA:UniProtKB-KW.
DR   GO; GO:0006541; P:glutamine metabolic process; IEA:UniProtKB-KW.
DR   CDD; cd01991; Asn_Synthase_B_C; 1.
DR   Gene3D; 3.40.50.620; -; 1.
DR   Gene3D; 3.60.20.10; -; 1.
DR   InterPro; IPR001962; Asn_synthase.
DR   InterPro; IPR017932; GATase_2_dom.
DR   InterPro; IPR029055; Ntn_hydrolases_N.
DR   InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR   Pfam; PF00733; Asn_synthase; 2.
DR   Pfam; PF13537; GATase_7; 1.
DR   SUPFAM; SSF56235; SSF56235; 1.
DR   PROSITE; PS51278; GATASE_TYPE_2; 1.
PE   2: Evidence at transcript level;
KW   Amino-acid biosynthesis; Asparagine biosynthesis;
KW   Glutamine amidotransferase; Reference proteome.
FT   INIT_MET        1
FT                   /note="Removed"
FT                   /evidence="ECO:0000250"
FT   CHAIN           2..612
FT                   /note="Asparagine synthetase domain-containing protein 1"
FT                   /id="PRO_0000324764"
FT   DOMAIN          2..183
FT                   /note="Glutamine amidotransferase type-2"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00609"
FT   DOMAIN          277..582
FT                   /note="Asparagine synthetase"
FT   ACT_SITE        2
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00609"
FT   CONFLICT        13..15
FT                   /note="QHN -> PHD (in Ref. 2; AAH51619)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        22..23
FT                   /note="HK -> LQ (in Ref. 2; AAH51619)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        38
FT                   /note="E -> K (in Ref. 2; AAH51619)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        83
FT                   /note="L -> I (in Ref. 2; AAH51619)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        151
FT                   /note="S -> F (in Ref. 2; AAH51619)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        155
FT                   /note="A -> V (in Ref. 2; AAH51619)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        219
FT                   /note="R -> K (in Ref. 2; AAH51619)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        258
FT                   /note="S -> T (in Ref. 2; AAH51619)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        372
FT                   /note="T -> M (in Ref. 2; AAH51619)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        375
FT                   /note="D -> G (in Ref. 2; AAH51619)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        382
FT                   /note="F -> S (in Ref. 2; AAH51619)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        500
FT                   /note="V -> I (in Ref. 2; AAH51619)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   612 AA;  68298 MW;  305E17CC50140CA2 CRC64;
     MCGICCVVSL TTQHNPLEEN VHKNLKHRGP NCSKDITEEI SNCSVLFSAH VLHMRGCLTP
     QPLQDDTGNM LLWNGEVFGG LKLEQEENDT EVLLHQLSMA TSASDIVCLL SQLEGPWAFI
     YYQKSERCIW FGRDFFGRRS LLWKFNPERA SFTLASVASQ PADDSQSQWQ EVPPGGVYRF
     DLTDTSLDGT FIFELYPWIF HSDDKPIESP ELKCEGLPRC VSVNTNSSGL YLTSPVCPMN
     TSISSLTTEQ NHNASQTSVE NLKAFLTNTD KKAMVSALID VLSEAVRKRV QYLPARDALE
     VSLNQTKADI AILFSGGIDS MILAALTDRH VPADKPIDLL NVAFKMQEAK MPPKSMKKGK
     NKKNDCDDAD KTQLDQQKFN PFNVPDRITG RAGLQELKNL SPKRQWNFIE INVTQEELVE
     MRQKRICHLV HPLDTVLDDS LGCAIWFAAR GIGVINEAVD EELYISEAKV VLTGIGADEQ
     LAGYSRHRVR YKHSGLEGLV RELAMELGRI SSRNLGRDDR IIGDHGKEAR FPYLDEDVVS
     FLNGLPVSEK ADLSLPRGVG EKLLLRLAAV ELGLGLSALL PKRAMQFGSR IAKLEDNHEK
     ASDKCKRLVT TI