ID   ASND1_DROME             Reviewed;         564 AA.
AC   Q5LJP9; Q9W5U0;
DT   18-MAR-2008, integrated into UniProtKB/Swiss-Prot.
DT   18-MAR-2008, sequence version 2.
DT   03-AUG-2022, entry version 100.
DE   RecName: Full=Asparagine synthetase domain-containing protein CG17486;
GN   ORFNames=CG17486;
OS   Drosophila melanogaster (Fruit fly).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC   Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC   Drosophilidae; Drosophila; Sophophora.
OX   NCBI_TaxID=7227;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Berkeley;
RX   PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA   Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA   Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA   George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA   Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA   Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA   Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA   An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA   Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA   Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA   Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA   Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA   Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA   Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA   Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA   Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA   Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA   Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA   Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA   Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA   Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA   Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA   McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA   Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA   Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA   Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA   Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA   Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA   Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA   Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA   Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA   Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA   Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA   Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA   Venter J.C.;
RT   "The genome sequence of Drosophila melanogaster.";
RL   Science 287:2185-2195(2000).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=Berkeley;
RX   PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA   Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA   Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA   Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA   Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA   Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA   Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT   "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT   review.";
RL   Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=Berkeley; TISSUE=Embryo;
RX   PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA   Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA   Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA   Celniker S.E.;
RT   "A Drosophila full-length cDNA resource.";
RL   Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
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DR   EMBL; AE013599; EAA45987.3; -; Genomic_DNA.
DR   EMBL; AY069456; AAL39601.1; -; mRNA.
DR   RefSeq; NP_001036446.2; NM_001042981.3.
DR   AlphaFoldDB; Q5LJP9; -.
DR   SMR; Q5LJP9; -.
DR   BioGRID; 78263; 1.
DR   DIP; DIP-23653N; -.
DR   IntAct; Q5LJP9; 1.
DR   STRING; 7227.FBpp0289202; -.
DR   PaxDb; Q5LJP9; -.
DR   PRIDE; Q5LJP9; -.
DR   DNASU; 3355138; -.
DR   EnsemblMetazoa; FBtr0299924; FBpp0289202; FBgn0032997.
DR   GeneID; 3355138; -.
DR   KEGG; dme:Dmel_CG17486; -.
DR   UCSC; CG17486-RB; d. melanogaster.
DR   FlyBase; FBgn0032997; CG17486.
DR   VEuPathDB; VectorBase:FBgn0032997; -.
DR   eggNOG; KOG0573; Eukaryota.
DR   GeneTree; ENSGT00390000012446; -.
DR   HOGENOM; CLU_012368_2_0_1; -.
DR   InParanoid; Q5LJP9; -.
DR   OMA; LEMDWQR; -.
DR   OrthoDB; 83779at2759; -.
DR   PhylomeDB; Q5LJP9; -.
DR   BioGRID-ORCS; 3355138; 1 hit in 1 CRISPR screen.
DR   GenomeRNAi; 3355138; -.
DR   PRO; PR:Q5LJP9; -.
DR   Proteomes; UP000000803; Chromosome 2R.
DR   Bgee; FBgn0032997; Expressed in egg cell and 23 other tissues.
DR   Genevisible; Q5LJP9; DM.
DR   GO; GO:0004066; F:asparagine synthase (glutamine-hydrolyzing) activity; IEA:InterPro.
DR   GO; GO:0006529; P:asparagine biosynthetic process; IEA:UniProtKB-KW.
DR   GO; GO:0006541; P:glutamine metabolic process; IEA:UniProtKB-KW.
DR   CDD; cd01991; Asn_Synthase_B_C; 1.
DR   Gene3D; 3.40.50.620; -; 1.
DR   Gene3D; 3.60.20.10; -; 1.
DR   InterPro; IPR001962; Asn_synthase.
DR   InterPro; IPR017932; GATase_2_dom.
DR   InterPro; IPR029055; Ntn_hydrolases_N.
DR   InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR   Pfam; PF00733; Asn_synthase; 1.
DR   Pfam; PF13537; GATase_7; 1.
DR   SUPFAM; SSF56235; SSF56235; 1.
PE   2: Evidence at transcript level;
KW   Amino-acid biosynthesis; Asparagine biosynthesis;
KW   Glutamine amidotransferase; Reference proteome.
FT   INIT_MET        1
FT                   /note="Removed"
FT                   /evidence="ECO:0000250"
FT   CHAIN           2..564
FT                   /note="Asparagine synthetase domain-containing protein
FT                   CG17486"
FT                   /id="PRO_0000324765"
FT   DOMAIN          2..180
FT                   /note="Glutamine amidotransferase type-2"
FT   DOMAIN          280..541
FT                   /note="Asparagine synthetase"
FT   ACT_SITE        2
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   564 AA;  64746 MW;  6AE0CB400C0B34E3 CRC64;
     MCGIFCSVVN NVPLNSFNIS SALKVILKNR GPDVQDEVVI DYCFGKILFA GFVLWQQGES
     VQKQPVVEDD FIFLFNGDIY NTSKPEYMSD TTWIAERLAE CRCKEQNILK ILKRLEGPHC
     LIIYDKREQI LYFSRDALGR NSLLIERICN GFQLLSTSHF EDKKISLELP PLGLFRVKLN
     DLNSCVLYPW QPLNNYSTQL LRNLDLAIGW KTAVESPMSP DWMLNCNPAF SYNFYEFQYI
     KNNVNLYKNL IDQSEIKYTL SILHTLLSDS IKNRVRNMAP FCRLCMQKLN ISPPCRHAKL
     CILFSGGLDC TILALLANQF VPVNEPIELI NVAFESVKGQ NISEKLFDVP DRKTSLVSVN
     ELKQLCPERC WNLLKVNVTR LELQQHLTSR IKHLIYPLDT VLDESLGCAF WFASHCTHST
     ARVALIGSGA DELFGGYTRY RNSYSRCLGN DLERQLAVQN ELERDWQRIS ARNLARDDRI
     IADTGKTARS PFIEENFVKF IRSLEVYQKC CFGFPEGVGD KLLLRLYGYQ IGLRDVVFLK
     KRAIQFGSRI ANKKQNGSHQ SDNL