PAPH_ECOLX
ID PAPH_ECOLX Reviewed; 195 AA.
AC P07111;
DT 01-APR-1988, integrated into UniProtKB/Swiss-Prot.
DT 01-APR-1988, sequence version 1.
DT 25-MAY-2022, entry version 98.
DE RecName: Full=PAP fimbrial minor pilin protein;
DE Flags: Precursor;
GN Name=papH;
OS Escherichia coli.
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=562;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 700336 / J96 / UPEC;
RX PubMed=2882856; DOI=10.1016/0092-8674(87)90565-4;
RA Baga M., Norgren M., Normark S.;
RT "Biogenesis of E. coli Pap pili: papH, a minor pilin subunit involved in
RT cell anchoring and length modulation.";
RL Cell 49:241-251(1987).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 700336 / J96 / UPEC;
RX PubMed=1357526; DOI=10.1111/j.1365-2958.1992.tb01399.x;
RA Marklund B.-I., Tennent J.M., Garcia E., Hamers A., Baga M., Lindberg F.,
RA Gaastra W., Normark S.;
RT "Horizontal gene transfer of the Escherichia coli pap and prs pili operons
RT as a mechanism for the development of tissue-specific adhesive
RT properties.";
RL Mol. Microbiol. 6:2225-2242(1992).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 149-195.
RC STRAIN=ATCC 700336 / J96 / UPEC;
RX PubMed=2897064; DOI=10.1111/j.1365-2958.1987.tb00509.x;
RA Norgren M., Baga M., Tennent J.M., Normark S.;
RT "Nucleotide sequence, regulation and functional analysis of the papC gene
RT required for cell surface localization of Pap pili of uropathogenic
RT Escherichia coli.";
RL Mol. Microbiol. 1:169-178(1987).
RN [4]
RP X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS) OF 23-195, AND DISULFIDE BOND.
RX PubMed=17082819; DOI=10.1038/sj.embor.7400833;
RA Verger D., Miller E., Remaut H., Waksman G., Hultgren S.;
RT "Molecular mechanism of P pilus termination in uropathogenic Escherichia
RT coli.";
RL EMBO Rep. 7:1228-1232(2006).
CC -!- FUNCTION: Fimbriae (also called pili), polar filaments radiating from
CC the surface of the bacterium to a length of 0.5-1.5 micrometers and
CC numbering 100-300 per cell, enable bacteria to colonize the epithelium
CC of specific host organs.
CC -!- FUNCTION: PapH seems to anchor the pilus to the bacterial cell. In
CC addition the stoichiometric relationship between PapH and PapA
CC determines the pilus length.
CC -!- SUBCELLULAR LOCATION: Secreted. Fimbrium.
CC -!- MISCELLANEOUS: Strains of E.coli that cause infection of the human
CC urinary tract produce pap-pili which are hair-like appendages
CC consisting of about 1000 helically arranged subunits of the protein
CC PapA. These pili mediate binding to digalactoside-containing
CC glycolipids present on the epithelial cells which line the urinary
CC tract.
CC -!- SIMILARITY: Belongs to the fimbrial protein family. {ECO:0000305}.
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DR EMBL; Y00529; CAA68587.1; -; Genomic_DNA.
DR EMBL; M16202; AAA24286.1; -; Genomic_DNA.
DR EMBL; X61239; CAA43563.1; -; Genomic_DNA.
DR PIR; A27021; YQECPH.
DR RefSeq; WP_001239368.1; NZ_VOHF01000048.1.
DR PDB; 2J2Z; X-ray; 2.30 A; B=23-195.
DR PDB; 2XG4; X-ray; 2.40 A; B=23-195.
DR PDB; 2XG5; X-ray; 2.00 A; B=23-195.
DR PDBsum; 2J2Z; -.
DR PDBsum; 2XG4; -.
DR PDBsum; 2XG5; -.
DR AlphaFoldDB; P07111; -.
DR SMR; P07111; -.
DR DIP; DIP-44596N; -.
DR IntAct; P07111; 3.
DR MINT; P07111; -.
DR EvolutionaryTrace; P07111; -.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0009289; C:pilus; IEA:UniProtKB-SubCell.
DR GO; GO:0007155; P:cell adhesion; IEA:InterPro.
DR Gene3D; 2.60.40.1090; -; 1.
DR InterPro; IPR036937; Adhesion_dom_fimbrial_sf.
DR InterPro; IPR008966; Adhesion_dom_sf.
DR SUPFAM; SSF49401; SSF49401; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Disulfide bond; Fimbrium; Secreted; Signal.
FT SIGNAL 1..22
FT CHAIN 23..195
FT /note="PAP fimbrial minor pilin protein"
FT /id="PRO_0000009198"
FT DISULFID 58..97
FT /evidence="ECO:0000269|PubMed:17082819"
FT STRAND 59..61
FT /evidence="ECO:0007829|PDB:2XG5"
FT STRAND 68..70
FT /evidence="ECO:0007829|PDB:2XG5"
FT HELIX 76..80
FT /evidence="ECO:0007829|PDB:2XG5"
FT STRAND 88..97
FT /evidence="ECO:0007829|PDB:2XG5"
FT TURN 106..110
FT /evidence="ECO:0007829|PDB:2XG5"
FT STRAND 111..118
FT /evidence="ECO:0007829|PDB:2XG5"
FT STRAND 130..132
FT /evidence="ECO:0007829|PDB:2J2Z"
FT STRAND 134..142
FT /evidence="ECO:0007829|PDB:2XG5"
FT STRAND 165..174
FT /evidence="ECO:0007829|PDB:2XG5"
FT STRAND 187..194
FT /evidence="ECO:0007829|PDB:2XG5"
SQ SEQUENCE 195 AA; 21835 MW; D2120FC5DA063169 CRC64;
MRLRFSVPLF FFGCVFVHGV FAGPFPPPGM SLPEYWGEEH VWWDGRAAFH GEVVRPACTL
AMEDAWQIID MGETPVRDLQ NGFSGPERKF SLRLRNCEFN SQGGNLFSDS RIRVTFDGVR
GETPDKFNLS GQAKGINLQI ADVRGNIARA GKVMPAIPLT GNEEALDYTL RIVRNGKKLE
AGNYFAVLGF RVDYE
