A36DE_DROME
ID A36DE_DROME Reviewed; 912 AA.
AC Q9V3R1; O46198; Q9U9Y5;
DT 15-NOV-2002, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 142.
DE RecName: Full=Accessory gland protein Acp36DE;
DE Flags: Precursor;
GN Name=Acp36DE; ORFNames=CG7157;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227 {ECO:0000312|EMBL:AAG37359.1};
RN [1] {ECO:0000305}
RP NUCLEOTIDE SEQUENCE, FUNCTION, AND TISSUE SPECIFICITY.
RC STRAIN=Canton-S; TISSUE=Male accessory gland;
RX PubMed=9474779; DOI=10.1016/s0965-1748(97)00056-8;
RA Wolfner M.F., Harada H.A., Bertram M.J., Stelick T.J., Kraus K.W.,
RA Kalb J.M., Lung Y.O., Neubaum D.M., Park M., Tram U.K.;
RT "New genes for male accessory gland proteins in Drosophila melanogaster.";
RL Insect Biochem. Mol. Biol. 27:825-834(1997).
RN [2] {ECO:0000305, ECO:0000312|EMBL:AAM29349.1}
RP SEQUENCE REVISION TO N-TERMINUS AND C-TERMINUS.
RA Wolfner M.F., Harada H.A., Bertram M.J., Stelick T.J., Kraus K.W.,
RA Kalb J.M., Lung Y.O., Neubaum D.M., Park M., Tram U.K.;
RL Submitted (JUN-1999) to the EMBL/GenBank/DDBJ databases.
RN [3] {ECO:0000305}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley; TISSUE=Head;
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
RN [6] {ECO:0000305}
RP NUCLEOTIDE SEQUENCE OF 144-889.
RC STRAIN=SENGWA2, SENGWA22, SENGWA29, SENGWA30, SENGWA32, SENGWA37, SENGWA51,
RC WSII1, WSII12, WSII16, WSII19, WSII26, WSII47, WSII49, WSII6, and WSII9;
RX PubMed=11102381; DOI=10.1093/genetics/156.4.1879;
RA Begun D.J., Whitley P., Todd B.L., Waldrip-Dail H.M., Clark A.G.;
RT "Molecular population genetics of male accessory gland proteins in
RT Drosophila.";
RL Genetics 156:1879-1888(2000).
RN [7] {ECO:0000305}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 401-912.
RC STRAIN=Berkeley; TISSUE=Head;
RX PubMed=10731138; DOI=10.1126/science.287.5461.2222;
RA Rubin G.M., Hong L., Brokstein P., Evans-Holm M., Frise E., Stapleton M.,
RA Harvey D.A.;
RT "A Drosophila complementary DNA resource.";
RL Science 287:2222-2224(2000).
RN [8]
RP FUNCTION.
RX PubMed=12939382; DOI=10.1242/jeb.00585;
RA Bloch-Qazi M.C., Wolfner M.F.;
RT "An early role for the Drosophila melanogaster male seminal protein Acp36DE
RT in female sperm storage.";
RL J. Exp. Biol. 206:3521-3528(2003).
RN [9]
RP TISSUE SPECIFICITY, AND PROTEOLYTIC PROCESSING BY SEMP1.
RX PubMed=24514904; DOI=10.1534/genetics.113.160101;
RA Laflamme B.A., Avila F.W., Michalski K., Wolfner M.F.;
RT "A Drosophila protease cascade member, seminal metalloprotease-1, is
RT activated stepwise by male factors and requires female factors for full
RT activity.";
RL Genetics 196:1117-1129(2014).
CC -!- FUNCTION: Responsible for physiological and behavioral changes in mated
CC female flies. Associates with sperm and localizes to specific regions
CC of the female reproductive tract, including the sperm storage organs.
CC It accelerates sperm accumulation into storage but does not mediate the
CC entry of the first sperm into storage. Once sperm storage has initiated
CC it seems to act as a guidance factor helping subsequent sperm move into
CC storage, a corral concentrating sperm around the SSO entrances and/or a
CC trigger for responses within the female that accelerate storage of
CC sperm. {ECO:0000269|PubMed:12939382, ECO:0000269|PubMed:9474779}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000305}.
CC -!- TISSUE SPECIFICITY: Detected in the male accessory glands (at protein
CC level) (PubMed:24514904). Produced in the accessory glands and secreted
CC into seminal fluid (PubMed:9474779). {ECO:0000269|PubMed:24514904,
CC ECO:0000269|PubMed:9474779}.
CC -!- PTM: Proteolytically cleaved by the seminal metalloprotease Semp1
CC (PubMed:24514904). Cleavage appears to take place in the mated female
CC (PubMed:24514904). {ECO:0000269|PubMed:24514904}.
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DR EMBL; U85759; AAB96383.2; -; mRNA.
DR EMBL; AF157488; AAD40185.1; -; Genomic_DNA.
DR EMBL; AE014134; AAF53664.1; -; Genomic_DNA.
DR EMBL; AY113344; AAM29349.1; -; mRNA.
DR EMBL; AY010577; AAG37359.1; -; Genomic_DNA.
DR EMBL; AY010578; AAG37360.1; -; Genomic_DNA.
DR EMBL; AY010579; AAG37361.1; -; Genomic_DNA.
DR EMBL; AY010580; AAG37362.1; -; Genomic_DNA.
DR EMBL; AY010581; AAG37363.1; -; Genomic_DNA.
DR EMBL; AY010582; AAG37364.1; -; Genomic_DNA.
DR EMBL; AY010583; AAG37365.1; -; Genomic_DNA.
DR EMBL; AY010584; AAG37366.1; -; Genomic_DNA.
DR EMBL; AY010585; AAG37367.1; -; Genomic_DNA.
DR EMBL; AY010586; AAG37368.1; -; Genomic_DNA.
DR EMBL; AY010587; AAG37369.1; -; Genomic_DNA.
DR EMBL; AY010588; AAG37370.1; -; Genomic_DNA.
DR EMBL; AY010589; AAG37371.1; -; Genomic_DNA.
DR EMBL; AY010590; AAG37372.1; -; Genomic_DNA.
DR EMBL; AY010591; AAG37373.1; -; Genomic_DNA.
DR EMBL; AY010592; AAG37374.1; -; Genomic_DNA.
DR EMBL; AF145666; AAD38641.1; -; mRNA.
DR RefSeq; NP_001286040.1; NM_001299111.1.
DR RefSeq; NP_523594.1; NM_078870.3.
DR AlphaFoldDB; Q9V3R1; -.
DR BioGRID; 61091; 2.
DR DIP; DIP-24017N; -.
DR IntAct; Q9V3R1; 8.
DR MINT; Q9V3R1; -.
DR STRING; 7227.FBpp0080607; -.
DR PaxDb; Q9V3R1; -.
DR PRIDE; Q9V3R1; -.
DR EnsemblMetazoa; FBtr0081054; FBpp0080607; FBgn0011559.
DR EnsemblMetazoa; FBtr0340581; FBpp0309468; FBgn0011559.
DR GeneID; 35096; -.
DR KEGG; dme:Dmel_CG7157; -.
DR CTD; 35096; -.
DR FlyBase; FBgn0011559; Acp36DE.
DR VEuPathDB; VectorBase:FBgn0011559; -.
DR HOGENOM; CLU_357253_0_0_1; -.
DR OMA; IHELYQN; -.
DR OrthoDB; 1590807at2759; -.
DR PhylomeDB; Q9V3R1; -.
DR SignaLink; Q9V3R1; -.
DR BioGRID-ORCS; 35096; 0 hits in 1 CRISPR screen.
DR GenomeRNAi; 35096; -.
DR PRO; PR:Q9V3R1; -.
DR Proteomes; UP000000803; Chromosome 2L.
DR Bgee; FBgn0011559; Expressed in male reproductive gland and 13 other tissues.
DR ExpressionAtlas; Q9V3R1; baseline and differential.
DR Genevisible; Q9V3R1; DM.
DR GO; GO:0005576; C:extracellular region; ISM:FlyBase.
DR GO; GO:0005615; C:extracellular space; IDA:FlyBase.
DR GO; GO:0005179; F:hormone activity; NAS:UniProtKB.
DR GO; GO:0042628; P:mating plug formation; TAS:FlyBase.
DR GO; GO:0045434; P:negative regulation of female receptivity, post-mating; NAS:UniProtKB.
DR GO; GO:0019953; P:sexual reproduction; IEP:FlyBase.
DR GO; GO:0046692; P:sperm competition; IDA:FlyBase.
DR GO; GO:0046693; P:sperm storage; IMP:FlyBase.
DR InterPro; IPR006595; CTLH_C.
PE 1: Evidence at protein level;
KW Behavior; Reference proteome; Secreted; Signal.
FT SIGNAL 1..23
FT /evidence="ECO:0000305"
FT CHAIN 24..912
FT /note="Accessory gland protein Acp36DE"
FT /id="PRO_0000020583"
FT REGION 193..255
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 271..349
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 518..544
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 638..671
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 732..912
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 732..804
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 814..912
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VARIANT 148
FT /note="N -> S (in strain: WSII16)"
FT /evidence="ECO:0000305"
FT VARIANT 190
FT /note="A -> V (in strain: WSII16)"
FT /evidence="ECO:0000305"
FT VARIANT 198
FT /note="T -> S (in strain: SENGWA2, SENGWA22, SENGWA29,
FT SENGWA32, SENGWA37, SENGWA51, WSII19 and WSII49)"
FT /evidence="ECO:0000305"
FT VARIANT 223
FT /note="Missing (in strain: SENGWA22)"
FT /evidence="ECO:0000305"
FT VARIANT 231
FT /note="E -> G (in strain: WSII6)"
FT /evidence="ECO:0000305"
FT VARIANT 253
FT /note="Q -> QSQ (in strain: SENGWA2, SENGWA29, SENGWA32,
FT SENGWA51, WSII19 and WSII49)"
FT /evidence="ECO:0000305"
FT VARIANT 307
FT /note="E -> G (in strain: WSII6)"
FT /evidence="ECO:0000305"
FT VARIANT 316
FT /note="I -> F (in strain: WSII16)"
FT /evidence="ECO:0000305"
FT VARIANT 325
FT /note="K -> T (in strain: SENGWA37)"
FT /evidence="ECO:0000305"
FT VARIANT 335
FT /note="S -> P (in strain: SENGWA30)"
FT /evidence="ECO:0000305"
FT VARIANT 352
FT /note="Q -> QLLREAQQK (in strain: SENGWA2, SENGWA22,
FT SENGWA29, SENGWA30, SENGWA32, SENGWA37, SENGWA51, WSII16,
FT WSII19 and WSII49)"
FT /evidence="ECO:0000305"
FT VARIANT 385
FT /note="F -> HQN (in strain: WSII1)"
FT /evidence="ECO:0000305"
FT VARIANT 430
FT /note="V -> I (in strain: WSII16)"
FT /evidence="ECO:0000305"
FT VARIANT 464
FT /note="L -> F (in strain: Canton-S, SENGWA2, SENGWA22,
FT SENGWA29, SENGWA32 and SENGWA51)"
FT VARIANT 496
FT /note="E -> G (in strain: WSII6)"
FT /evidence="ECO:0000305"
FT VARIANT 507
FT /note="L -> V (in strain: SENGWA22, SENGWA29, SENGWA37 and
FT SENGWA51)"
FT /evidence="ECO:0000305"
FT VARIANT 575
FT /note="L -> Q (in strain: WSII16)"
FT /evidence="ECO:0000305"
FT VARIANT 601
FT /note="S -> I (in strain: WSII26 and WSII49)"
FT /evidence="ECO:0000305"
FT VARIANT 613
FT /note="E -> K (in strain: SENGWA30)"
FT /evidence="ECO:0000305"
FT VARIANT 650
FT /note="P -> A (in strain: SENGWA22, SENGWA30 and WSII16)"
FT /evidence="ECO:0000305"
FT VARIANT 666
FT /note="L -> V (in strain: SENGWA29, SENGWA30, SENGWA37 and
FT SENGWA51)"
FT /evidence="ECO:0000305"
FT VARIANT 670
FT /note="G -> E (in strain: SENGWA29, SENGWA30, SENGWA37 and
FT SENGWA51)"
FT /evidence="ECO:0000305"
FT VARIANT 758
FT /note="T -> A (in strain: SENGWA37 and SENGWA51)"
FT /evidence="ECO:0000305"
FT VARIANT 782..786
FT /note="Missing (in strain: SENGWA37 and SENGWA51)"
FT /evidence="ECO:0000305"
FT VARIANT 830
FT /note="Q -> E (in strain: WSII26)"
FT /evidence="ECO:0000305"
FT VARIANT 844
FT /note="Q -> R (in strain: WSII16)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 912 AA; 101921 MW; 81E2C53431EAFBC5 CRC64;
MWTLTCQQFI ALILLGTLVP SESFLCKHCF RKNLEKVHES FRDILSPPIF GVNPQPLIEV
QQPKVTPKPE SSQVIHVHQP QVILKPIYYP KVDTISTKNQ IGIHGPYSQY PSLLPSANLL
GIPNQQLINA QDVLSDKDQK QTQVQNNNLH IRFGVSALRE GRNNPSLETI SRDKVDKISP
ALQLQLLRYA DSQSQSQTQS QSASQSESNA SSQFQAQEQS NRLLENPPVS ESQSQSESQS
QSESQKQSQS QSQRQQQIQT QLQILRQLQQ KSNEQSAAQS ASQIQSQRQS DSQSNLQLQE
QSQSQSEQGK PIQSQIQILQ GLQQKELDDK SASQSQSESK TRQEQQKQLN LQQLEELSSS
LSQSRLGLGQ QIQSQLQKNQ LDKQFASQFQ SQSKSQLEQQ MQLQLQSLRQ LQQKQLDEQS
ASQSQPQSQV AQQIQSHLQL LRLLQSRLKT QSALKSDLEQ QILLQLKKLT EVQQKQLAEQ
PTLRPSSKSQ SPGQLEQQIL LQLQNLLQFQ QNQLKSDTQT QSQLQESKSN SLSQSQSQSQ
EQLQLQRDQN LRQLEQIKLE MQNIRELLQK GKSELQTQSD SQRRIHELYQ NILQLNKEKL
SYQLKQLKLK ELEDQKKSQA EISKGSNPSN LFIIGQLPSE GKPAPGNQGP SIEPKLVPQP
GSLDKLPSGG GLIGKPASTG LYILSPDFND LSDYRDQFRL QQELKKHQNI LSLLQRRQND
IKKQQNAQLL LGQQQKEQQA QESINKQQSS SAGSSSQTKL QQDIQSTGAQ GSQQGLQAGS
TGLQTSSLQG TESSASQSAA LQRLKEQEQL RIQTENDQKT SSSSSHSNSQ NSQSSSSQSS
QASQSEAQRQ EAGNRNTLLL DQSSSKTQSE SKSESSSQSS SHSSSQSTSN SSSNVQSKLQ
GESQALLNNL SG
