PAQR8_HUMAN
ID PAQR8_HUMAN Reviewed; 354 AA.
AC Q8TEZ7; B2RCF6; Q86WL0; Q8N6D3; Q9HD02;
DT 16-AUG-2004, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2002, sequence version 1.
DT 03-AUG-2022, entry version 136.
DE RecName: Full=Membrane progestin receptor beta {ECO:0000303|PubMed:23763432};
DE Short=mPR beta {ECO:0000303|PubMed:23763432};
DE AltName: Full=Lysosomal membrane protein in brain 1 {ECO:0000303|PubMed:11676489};
DE AltName: Full=Membrane progesterone P4 receptor beta {ECO:0000303|PubMed:23763432};
DE AltName: Full=Membrane progesterone receptor beta {ECO:0000303|PubMed:23763432};
DE AltName: Full=Progesterone and adipoQ receptor family member 8;
DE AltName: Full=Progestin and adipoQ receptor family member 8 {ECO:0000303|PubMed:16044242};
DE AltName: Full=Progestin and adipoQ receptor family member VIII;
GN Name=PAQR8 {ECO:0000312|HGNC:HGNC:15708};
GN Synonyms=C6orf33 {ECO:0000312|HGNC:HGNC:15708}, LMPB1, MPRB;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, AND SUBCELLULAR LOCATION.
RC TISSUE=Brain;
RX PubMed=11676489; DOI=10.1006/bbrc.2001.5825;
RA Suzuki T., Ganesh S., Agarwala K.L., Morita R., Sugimoto Y., Inazawa J.,
RA Delgado-Escueta A.V., Yamakawa K.;
RT "A novel gene in the chromosomal region for juvenile myoclonic epilepsy on
RT 6p12 encodes a brain-specific lysosomal membrane protein.";
RL Biochem. Biophys. Res. Commun. 288:626-636(2001).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA], AND TISSUE SPECIFICITY.
RX PubMed=16044242; DOI=10.1007/s00239-004-0375-2;
RA Tang Y.T., Hu T., Arterburn M., Boyle B., Bright J.M., Emtage P.C.,
RA Funk W.D.;
RT "PAQR proteins: a novel membrane receptor family defined by an ancient 7-
RT transmembrane pass motif.";
RL J. Mol. Evol. 61:372-380(2005).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Placenta;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=14574404; DOI=10.1038/nature02055;
RA Mungall A.J., Palmer S.A., Sims S.K., Edwards C.A., Ashurst J.L.,
RA Wilming L., Jones M.C., Horton R., Hunt S.E., Scott C.E., Gilbert J.G.R.,
RA Clamp M.E., Bethel G., Milne S., Ainscough R., Almeida J.P., Ambrose K.D.,
RA Andrews T.D., Ashwell R.I.S., Babbage A.K., Bagguley C.L., Bailey J.,
RA Banerjee R., Barker D.J., Barlow K.F., Bates K., Beare D.M., Beasley H.,
RA Beasley O., Bird C.P., Blakey S.E., Bray-Allen S., Brook J., Brown A.J.,
RA Brown J.Y., Burford D.C., Burrill W., Burton J., Carder C., Carter N.P.,
RA Chapman J.C., Clark S.Y., Clark G., Clee C.M., Clegg S., Cobley V.,
RA Collier R.E., Collins J.E., Colman L.K., Corby N.R., Coville G.J.,
RA Culley K.M., Dhami P., Davies J., Dunn M., Earthrowl M.E., Ellington A.E.,
RA Evans K.A., Faulkner L., Francis M.D., Frankish A., Frankland J.,
RA French L., Garner P., Garnett J., Ghori M.J., Gilby L.M., Gillson C.J.,
RA Glithero R.J., Grafham D.V., Grant M., Gribble S., Griffiths C.,
RA Griffiths M.N.D., Hall R., Halls K.S., Hammond S., Harley J.L., Hart E.A.,
RA Heath P.D., Heathcott R., Holmes S.J., Howden P.J., Howe K.L., Howell G.R.,
RA Huckle E., Humphray S.J., Humphries M.D., Hunt A.R., Johnson C.M.,
RA Joy A.A., Kay M., Keenan S.J., Kimberley A.M., King A., Laird G.K.,
RA Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C.R.,
RA Lloyd D.M., Loveland J.E., Lovell J., Martin S., Mashreghi-Mohammadi M.,
RA Maslen G.L., Matthews L., McCann O.T., McLaren S.J., McLay K., McMurray A.,
RA Moore M.J.F., Mullikin J.C., Niblett D., Nickerson T., Novik K.L.,
RA Oliver K., Overton-Larty E.K., Parker A., Patel R., Pearce A.V., Peck A.I.,
RA Phillimore B.J.C.T., Phillips S., Plumb R.W., Porter K.M., Ramsey Y.,
RA Ranby S.A., Rice C.M., Ross M.T., Searle S.M., Sehra H.K., Sheridan E.,
RA Skuce C.D., Smith S., Smith M., Spraggon L., Squares S.L., Steward C.A.,
RA Sycamore N., Tamlyn-Hall G., Tester J., Theaker A.J., Thomas D.W.,
RA Thorpe A., Tracey A., Tromans A., Tubby B., Wall M., Wallis J.M.,
RA West A.P., White S.S., Whitehead S.L., Whittaker H., Wild A., Willey D.J.,
RA Wilmer T.E., Wood J.M., Wray P.W., Wyatt J.C., Young L., Younger R.M.,
RA Bentley D.R., Coulson A., Durbin R.M., Hubbard T., Sulston J.E., Dunham I.,
RA Rogers J., Beck S.;
RT "The DNA sequence and analysis of human chromosome 6.";
RL Nature 425:805-811(2003).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Brain;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [6]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 133-354, AND TISSUE SPECIFICITY.
RC TISSUE=Brain;
RX PubMed=12601167; DOI=10.1073/pnas.0436133100;
RA Zhu Y., Bond J., Thomas P.;
RT "Identification, classification, and partial characterization of genes in
RT humans and other vertebrates homologous to a fish membrane progestin
RT receptor.";
RL Proc. Natl. Acad. Sci. U.S.A. 100:2237-2242(2003).
RN [7]
RP REVIEW.
RX PubMed=12606724; DOI=10.1073/pnas.0530224100;
RA Hammes S.R.;
RT "The further redefining of steroid-mediated signaling.";
RL Proc. Natl. Acad. Sci. U.S.A. 100:2168-2170(2003).
RN [8]
RP FUNCTION, REVIEW, AND SUBCELLULAR LOCATION.
RX PubMed=23763432; DOI=10.1111/jne.12060;
RA Petersen S.L., Intlekofer K.A., Moura-Conlon P.J., Brewer D.N.,
RA Del Pino Sans J., Lopez J.A.;
RT "Nonclassical progesterone signalling molecules in the nervous system.";
RL J. Neuroendocrinol. 25:991-1001(2013).
RN [9]
RP FUNCTION, AND TISSUE SPECIFICITY.
RX PubMed=23161870; DOI=10.1210/en.2012-1772;
RA Pang Y., Dong J., Thomas P.;
RT "Characterization, neurosteroid binding and brain distribution of human
RT membrane progesterone receptors delta and {epsilon} (mPRdelta and
RT mPR{epsilon}) and mPRdelta involvement in neurosteroid inhibition of
RT apoptosis.";
RL Endocrinology 154:283-295(2013).
CC -!- FUNCTION: Plasma membrane progesterone (P4) receptor coupled to G
CC proteins (PubMed:23763432). Seems to act through a G(i) mediated
CC pathway (PubMed:23763432). May be involved in oocyte maturation (By
CC similarity). Also binds dehydroepiandrosterone (DHEA), pregnanolone,
CC pregnenolone and allopregnanolone (PubMed:23161870).
CC {ECO:0000250|UniProtKB:Q80ZE5, ECO:0000269|PubMed:23161870,
CC ECO:0000303|PubMed:23763432}.
CC -!- INTERACTION:
CC Q8TEZ7; Q08426: EHHADH; NbExp=3; IntAct=EBI-12847818, EBI-2339219;
CC Q8TEZ7; O95214: LEPROTL1; NbExp=3; IntAct=EBI-12847818, EBI-750776;
CC Q8TEZ7; Q00013: MPP1; NbExp=3; IntAct=EBI-12847818, EBI-711788;
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:11676489,
CC ECO:0000303|PubMed:23763432}; Multi-pass membrane protein
CC {ECO:0000255}. Note=Colocalizes with a lysosomal protein CTSD/cathepsin
CC D. {ECO:0000269|PubMed:11676489}.
CC -!- TISSUE SPECIFICITY: Highly expressed in the hypothalamus
CC (PubMed:23161870). Also expressed in spinal cord, kidney and testis.
CC {ECO:0000269|PubMed:11676489, ECO:0000269|PubMed:12601167,
CC ECO:0000269|PubMed:16044242, ECO:0000269|PubMed:23161870}.
CC -!- MISCELLANEOUS: Non-classical progesterone receptors involved in
CC extranuclear signaling are classified in 2 groups: the class II
CC progestin and adipoQ receptor (PAQR) family (also called mPRs) (PAQR5,
CC PAQR6, PAQR7, PAQR8 and PAQR9) and the b5-like heme/steroid-binding
CC protein family (also called MAPRs) (PGRMC1, PGRMC2, NENF and CYB5D2).
CC {ECO:0000303|PubMed:23763432}.
CC -!- SIMILARITY: Belongs to the ADIPOR family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAR08374.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; AF347029; AAL79777.2; -; mRNA.
DR EMBL; AY424286; AAR08374.1; ALT_INIT; mRNA.
DR EMBL; AK315088; BAG37553.1; -; mRNA.
DR EMBL; AL136125; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC030664; AAH30664.2; -; mRNA.
DR EMBL; AF313619; AAO47232.1; -; mRNA.
DR CCDS; CCDS4941.1; -.
DR RefSeq; NP_588608.1; NM_133367.4.
DR AlphaFoldDB; Q8TEZ7; -.
DR SMR; Q8TEZ7; -.
DR BioGRID; 124469; 3.
DR IntAct; Q8TEZ7; 3.
DR STRING; 9606.ENSP00000406197; -.
DR TCDB; 1.C.113.1.12; the hemolysin iii (hly iii) family.
DR iPTMnet; Q8TEZ7; -.
DR PhosphoSitePlus; Q8TEZ7; -.
DR BioMuta; PAQR8; -.
DR DMDM; 51316493; -.
DR MassIVE; Q8TEZ7; -.
DR PaxDb; Q8TEZ7; -.
DR PeptideAtlas; Q8TEZ7; -.
DR PRIDE; Q8TEZ7; -.
DR ProteomicsDB; 74529; -.
DR Antibodypedia; 30905; 115 antibodies from 23 providers.
DR DNASU; 85315; -.
DR Ensembl; ENST00000360726.3; ENSP00000353953.3; ENSG00000170915.9.
DR Ensembl; ENST00000442253.3; ENSP00000406197.2; ENSG00000170915.9.
DR GeneID; 85315; -.
DR KEGG; hsa:85315; -.
DR MANE-Select; ENST00000442253.3; ENSP00000406197.2; NM_133367.5; NP_588608.1.
DR UCSC; uc003pao.4; human.
DR CTD; 85315; -.
DR DisGeNET; 85315; -.
DR GeneCards; PAQR8; -.
DR HGNC; HGNC:15708; PAQR8.
DR HPA; ENSG00000170915; Tissue enhanced (brain).
DR MIM; 607780; gene.
DR neXtProt; NX_Q8TEZ7; -.
DR OpenTargets; ENSG00000170915; -.
DR PharmGKB; PA25934; -.
DR VEuPathDB; HostDB:ENSG00000170915; -.
DR eggNOG; KOG0748; Eukaryota.
DR GeneTree; ENSGT00940000159860; -.
DR HOGENOM; CLU_052356_0_0_1; -.
DR InParanoid; Q8TEZ7; -.
DR OMA; QYGCSLG; -.
DR OrthoDB; 1524940at2759; -.
DR PhylomeDB; Q8TEZ7; -.
DR TreeFam; TF319738; -.
DR PathwayCommons; Q8TEZ7; -.
DR SignaLink; Q8TEZ7; -.
DR BioGRID-ORCS; 85315; 18 hits in 1073 CRISPR screens.
DR ChiTaRS; PAQR8; human.
DR GeneWiki; PAQR8; -.
DR GenomeRNAi; 85315; -.
DR Pharos; Q8TEZ7; Tbio.
DR PRO; PR:Q8TEZ7; -.
DR Proteomes; UP000005640; Chromosome 6.
DR RNAct; Q8TEZ7; protein.
DR Bgee; ENSG00000170915; Expressed in inferior vagus X ganglion and 175 other tissues.
DR ExpressionAtlas; Q8TEZ7; baseline and differential.
DR Genevisible; Q8TEZ7; HS.
DR GO; GO:0005794; C:Golgi apparatus; IDA:HPA.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IDA:HPA.
DR GO; GO:0003707; F:nuclear steroid receptor activity; IBA:GO_Central.
DR GO; GO:0038023; F:signaling receptor activity; IBA:GO_Central.
DR GO; GO:0005496; F:steroid binding; IBA:GO_Central.
DR GO; GO:0048477; P:oogenesis; IEA:UniProtKB-KW.
DR GO; GO:0048545; P:response to steroid hormone; IBA:GO_Central.
DR InterPro; IPR004254; AdipoR/HlyIII-related.
DR PANTHER; PTHR20855; PTHR20855; 1.
DR Pfam; PF03006; HlyIII; 1.
PE 1: Evidence at protein level;
KW Cell membrane; Developmental protein; Differentiation; Lipid-binding;
KW Membrane; Oogenesis; Receptor; Reference proteome; Steroid-binding;
KW Transmembrane; Transmembrane helix.
FT CHAIN 1..354
FT /note="Membrane progestin receptor beta"
FT /id="PRO_0000218840"
FT TOPO_DOM 1..75
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 76..96
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TOPO_DOM 97..111
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 112..132
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TOPO_DOM 133..174
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 175..195
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255"
FT TOPO_DOM 196..213
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 214..234
FT /note="Helical; Name=4"
FT /evidence="ECO:0000255"
FT TOPO_DOM 235..243
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 244..264
FT /note="Helical; Name=5"
FT /evidence="ECO:0000255"
FT TOPO_DOM 265..283
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 284..304
FT /note="Helical; Name=6"
FT /evidence="ECO:0000255"
FT TOPO_DOM 305..319
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 320..340
FT /note="Helical; Name=7"
FT /evidence="ECO:0000255"
FT TOPO_DOM 341..354
FT /note="Extracellular"
FT /evidence="ECO:0000255"
SQ SEQUENCE 354 AA; 40464 MW; B0ECCD9C54C0CA24 CRC64;
MTTAILERLS TLSVSGQQLR RLPKILEDGL PKMPCTVPET DVPQLFREPY IRTGYRPTGH
EWRYYFFSLF QKHNEVVNVW THLLAALAVL LRFWAFAEAE ALPWASTHSL PLLLFILSSI
TYLTCSLLAH LLQSKSELSH YTFYFVDYVG VSVYQYGSAL AHFFYSSDQA WYDRFWLFFL
PAAAFCGWLS CAGCCYAKYR YRRPYPVMRK ICQVVPAGLA FILDISPVAH RVALCHLAGC
QEQAAWYHTL QILFFLVSAY FFSCPVPEKY FPGSCDIVGH GHQIFHAFLS ICTLSQLEAI
LLDYQGRQEI FLQRHGPLSV HMACLSFFFL AACSAATAAL LRHKVKARLT KKDS
