ASNS1_ORYSJ
ID ASNS1_ORYSJ Reviewed; 604 AA.
AC Q10MX3; A0A0P0VX10; Q0DSS5;
DT 09-JAN-2013, integrated into UniProtKB/Swiss-Prot.
DT 22-AUG-2006, sequence version 1.
DT 03-AUG-2022, entry version 92.
DE RecName: Full=Asparagine synthetase [glutamine-hydrolyzing] 1;
DE EC=6.3.5.4;
DE AltName: Full=Glutamine-dependent asparagine synthetase 1;
GN OrderedLocusNames=Os03g0291500, LOC_Os03g18130;
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16109971; DOI=10.1101/gr.3869505;
RG The rice chromosome 3 sequencing consortium;
RA Buell C.R., Yuan Q., Ouyang S., Liu J., Zhu W., Wang A., Maiti R., Haas B.,
RA Wortman J., Pertea M., Jones K.M., Kim M., Overton L., Tsitrin T.,
RA Fadrosh D., Bera J., Weaver B., Jin S., Johri S., Reardon M., Webb K.,
RA Hill J., Moffat K., Tallon L., Van Aken S., Lewis M., Utterback T.,
RA Feldblyum T., Zismann V., Iobst S., Hsiao J., de Vazeille A.R.,
RA Salzberg S.L., White O., Fraser C.M., Yu Y., Kim H., Rambo T., Currie J.,
RA Collura K., Kernodle-Thompson S., Wei F., Kudrna K., Ammiraju J.S.S.,
RA Luo M., Goicoechea J.L., Wing R.A., Henry D., Oates R., Palmer M.,
RA Pries G., Saski C., Simmons J., Soderlund C., Nelson W., de la Bastide M.,
RA Spiegel L., Nascimento L., Huang E., Preston R., Zutavern T., Palmer L.,
RA O'Shaughnessy A., Dike S., McCombie W.R., Minx P., Cordum H., Wilson R.,
RA Jin W., Lee H.R., Jiang J., Jackson S.;
RT "Sequence, annotation, and analysis of synteny between rice chromosome 3
RT and diverged grass species.";
RL Genome Res. 15:1284-1291(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Nipponbare;
RG The rice full-length cDNA consortium;
RT "Oryza sativa full length cDNA.";
RL Submitted (NOV-2008) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Essential for nitrogen assimilation, distribution and
CC remobilization within the plant via the phloem. {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O + L-aspartate + L-glutamine = AMP + diphosphate +
CC H(+) + L-asparagine + L-glutamate; Xref=Rhea:RHEA:12228,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:29985,
CC ChEBI:CHEBI:29991, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019,
CC ChEBI:CHEBI:58048, ChEBI:CHEBI:58359, ChEBI:CHEBI:456215; EC=6.3.5.4;
CC -!- PATHWAY: Amino-acid biosynthesis; L-asparagine biosynthesis.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAF11713.2; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AC137634; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; DP000009; ABF95401.1; -; Genomic_DNA.
DR EMBL; AP008209; BAF11713.2; ALT_SEQ; Genomic_DNA.
DR EMBL; AP014959; BAS83672.1; -; Genomic_DNA.
DR EMBL; AK318591; -; NOT_ANNOTATED_CDS; mRNA.
DR RefSeq; XP_015632089.1; XM_015776603.1.
DR AlphaFoldDB; Q10MX3; -.
DR SMR; Q10MX3; -.
DR STRING; 4530.OS03T0291500-01; -.
DR PaxDb; Q10MX3; -.
DR PRIDE; Q10MX3; -.
DR EnsemblPlants; Os03t0291500-01; Os03t0291500-01; Os03g0291500.
DR GeneID; 4332506; -.
DR Gramene; Os03t0291500-01; Os03t0291500-01; Os03g0291500.
DR KEGG; osa:4332506; -.
DR eggNOG; KOG0571; Eukaryota.
DR HOGENOM; CLU_014658_2_2_1; -.
DR InParanoid; Q10MX3; -.
DR OMA; FASKHTF; -.
DR OrthoDB; 782607at2759; -.
DR BRENDA; 6.3.5.4; 8948.
DR PlantReactome; R-OSA-1119354; Asparagine biosynthesis III.
DR PlantReactome; R-OSA-1119553; Asparagine biosynthesis.
DR UniPathway; UPA00134; -.
DR Proteomes; UP000000763; Chromosome 3.
DR Proteomes; UP000059680; Chromosome 3.
DR Genevisible; Q10MX3; OS.
DR GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR GO; GO:0004066; F:asparagine synthase (glutamine-hydrolyzing) activity; IBA:GO_Central.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0006529; P:asparagine biosynthetic process; IBA:GO_Central.
DR GO; GO:0006541; P:glutamine metabolic process; IEA:UniProtKB-KW.
DR GO; GO:0070981; P:L-asparagine biosynthetic process; IEA:UniProtKB-UniPathway.
DR CDD; cd01991; Asn_Synthase_B_C; 1.
DR CDD; cd00712; AsnB; 1.
DR Gene3D; 3.40.50.620; -; 1.
DR Gene3D; 3.60.20.10; -; 1.
DR InterPro; IPR006426; Asn_synth_AEB.
DR InterPro; IPR001962; Asn_synthase.
DR InterPro; IPR033738; AsnB_N.
DR InterPro; IPR017932; GATase_2_dom.
DR InterPro; IPR029055; Ntn_hydrolases_N.
DR InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR Pfam; PF00733; Asn_synthase; 1.
DR Pfam; PF13537; GATase_7; 1.
DR PIRSF; PIRSF001589; Asn_synthetase_glu-h; 1.
DR SUPFAM; SSF56235; SSF56235; 1.
DR TIGRFAMs; TIGR01536; asn_synth_AEB; 1.
DR PROSITE; PS51278; GATASE_TYPE_2; 1.
PE 2: Evidence at transcript level;
KW Amino-acid biosynthesis; Asparagine biosynthesis; ATP-binding;
KW Glutamine amidotransferase; Ligase; Nucleotide-binding; Reference proteome.
FT INIT_MET 1
FT /note="Removed"
FT /evidence="ECO:0000250"
FT CHAIN 2..604
FT /note="Asparagine synthetase [glutamine-hydrolyzing] 1"
FT /id="PRO_0000420842"
FT DOMAIN 2..186
FT /note="Glutamine amidotransferase type-2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00609"
FT DOMAIN 211..451
FT /note="Asparagine synthetase"
FT ACT_SITE 2
FT /note="Nucleophile"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00609"
FT BINDING 50..54
FT /ligand="L-glutamine"
FT /ligand_id="ChEBI:CHEBI:58359"
FT /evidence="ECO:0000250"
FT BINDING 75..77
FT /ligand="L-glutamine"
FT /ligand_id="ChEBI:CHEBI:58359"
FT /evidence="ECO:0000250"
FT BINDING 99
FT /ligand="L-glutamine"
FT /ligand_id="ChEBI:CHEBI:58359"
FT /evidence="ECO:0000250"
FT BINDING 232
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000250"
FT BINDING 268
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000250"
FT BINDING 342..343
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000250"
FT SITE 344
FT /note="Important for beta-aspartyl-AMP intermediate
FT formation"
FT /evidence="ECO:0000250"
SQ SEQUENCE 604 AA; 67322 MW; 029B5770234C20FB CRC64;
MCGILAVLGA ADWSQAKRAH VLSCSRRLKH RGPDWSGLYQ CEGNFLAQQR LAIVSPLSGD
QPLYNADRTI VVVANGEIYN HKKIRKQFAS KHTFSTGSDC EVIIPLYEEY GEDFVDMLDG
VFAFVLYDTR TKTYMAARDA IGVNPLYIGR GSDGAVWISS EMKALNEDCV EFEIFPPGHL
YSSAAGGLRR WYKPQWFAEN VPATPYQPLL LREAFEKAVI KRLMTDVPFG VLLSGGLDSS
LVAAVTKRHL IKTEAAEKFG AELHSFVVGL EGSPDLIAAR EVADHLGTIH HEFHFTVQDG
IDAIEEVIYH DETYDVTTIR ASTPMFLMAR KIKALGVKMV LSGEGSDELL GGYLYFHFAP
NKEEFHKETC RKVKALHQYD CLRANKATSA WGLEVRVPFL DKEFINVAMS MDPEWKMYNA
DLGRIEKWVM RKAFDDEEHP YLPKHILYRQ KEQFSDGVGY NWIDGLKAFT EQQVSDEMMK
NAAKVYPHNT PVNKEAYYYR MIFERLFPQE SARETVPWGP SIACSTPAAI EWVEQWKASH
DPSGRLIASH NSASASANHT NHANANANGN SNGKANGNCA MAANGTNGVG LVVANGTANG
KMEA
