PAR6G_HUMAN
ID PAR6G_HUMAN Reviewed; 376 AA.
AC Q9BYG4; A8QM57;
DT 16-MAY-2003, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2001, sequence version 1.
DT 03-AUG-2022, entry version 157.
DE RecName: Full=Partitioning defective 6 homolog gamma;
DE Short=PAR-6 gamma;
DE AltName: Full=PAR6D;
GN Name=PARD6G; Synonyms=PAR6G;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), AND INTERACTION WITH RAC1; CDC42;
RP PRKCI AND PRKCZ.
RC TISSUE=Neuroblastoma;
RX PubMed=11260256; DOI=10.1046/j.1365-2443.2001.00404.x;
RA Noda Y., Takeya R., Ohno S., Naito S., Ito T., Sumimoto H.;
RT "Human homologues of the Caenorhabditis elegans cell polarity protein PAR6
RT as an adaptor that links the small GTPases Rac and Cdc42 to atypical
RT protein kinase C.";
RL Genes Cells 6:107-119(2001).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
RA Noda Y., Kohjima M., Izaki T., Sumimoto H.;
RT "Splicing variants of the cell polarity protein PAR-6.";
RL Submitted (MAY-2004) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=16177791; DOI=10.1038/nature03983;
RA Nusbaum C., Zody M.C., Borowsky M.L., Kamal M., Kodira C.D., Taylor T.D.,
RA Whittaker C.A., Chang J.L., Cuomo C.A., Dewar K., FitzGerald M.G., Yang X.,
RA Abouelleil A., Allen N.R., Anderson S., Bloom T., Bugalter B., Butler J.,
RA Cook A., DeCaprio D., Engels R., Garber M., Gnirke A., Hafez N., Hall J.L.,
RA Norman C.H., Itoh T., Jaffe D.B., Kuroki Y., Lehoczky J., Lui A.,
RA Macdonald P., Mauceli E., Mikkelsen T.S., Naylor J.W., Nicol R., Nguyen C.,
RA Noguchi H., O'Leary S.B., Piqani B., Smith C.L., Talamas J.A., Topham K.,
RA Totoki Y., Toyoda A., Wain H.M., Young S.K., Zeng Q., Zimmer A.R.,
RA Fujiyama A., Hattori M., Birren B.W., Sakaki Y., Lander E.S.;
RT "DNA sequence and analysis of human chromosome 18.";
RL Nature 437:551-555(2005).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC TISSUE=Placenta;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [5]
RP INTERACTION WITH RHOQ.
RX PubMed=10934474; DOI=10.1038/35019573;
RA Joberty G., Petersen C., Gao L., Macara I.G.;
RT "The cell-polarity protein Par6 links Par3 and atypical protein kinase C to
RT Cdc42.";
RL Nat. Cell Biol. 2:531-539(2000).
CC -!- FUNCTION: Adapter protein involved in asymmetrical cell division and
CC cell polarization processes. May play a role in the formation of
CC epithelial tight junctions. The PARD6-PARD3 complex links GTP-bound Rho
CC small GTPases to atypical protein kinase C proteins (By similarity).
CC {ECO:0000250}.
CC -!- SUBUNIT: Interacts with PARD3 (Probable). Interacts with GTP-bound
CC forms of CDC42, RHOQ/TC10 and RAC1. Interacts with the N-terminal part
CC of PRKCI and PRKCZ. {ECO:0000269|PubMed:10934474,
CC ECO:0000269|PubMed:11260256, ECO:0000305}.
CC -!- INTERACTION:
CC Q9BYG4; P60953: CDC42; NbExp=5; IntAct=EBI-295417, EBI-81752;
CC Q9BYG4; Q7KZI7: MARK2; NbExp=2; IntAct=EBI-295417, EBI-516560;
CC Q9BYG4; Q96L34: MARK4; NbExp=2; IntAct=EBI-295417, EBI-302319;
CC Q9BYG4; Q8TEW0: PARD3; NbExp=3; IntAct=EBI-295417, EBI-81968;
CC Q9BYG4; P41743: PRKCI; NbExp=7; IntAct=EBI-295417, EBI-286199;
CC Q9BYG4; Q05513: PRKCZ; NbExp=7; IntAct=EBI-295417, EBI-295351;
CC Q9BYG4; P63000: RAC1; NbExp=2; IntAct=EBI-295417, EBI-413628;
CC Q9BYG4; Q04917: YWHAH; NbExp=2; IntAct=EBI-295417, EBI-306940;
CC -!- SUBCELLULAR LOCATION: Cytoplasm. Cell membrane {ECO:0000250}. Cell
CC junction, tight junction {ECO:0000250}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q9BYG4-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q9BYG4-2; Sequence=VSP_047586, VSP_047587;
CC -!- TISSUE SPECIFICITY: Widely expressed, with a higher expression in fetal
CC and adult kidney.
CC -!- DOMAIN: The pseudo-CRIB domain together with the PDZ domain is required
CC for the interaction with Rho small GTPases. {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the PAR6 family. {ECO:0000305}.
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DR EMBL; AB044556; BAB40757.1; -; mRNA.
DR EMBL; AB178536; BAF92015.1; -; mRNA.
DR EMBL; AC139100; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC060797; AAH60797.1; -; mRNA.
DR CCDS; CCDS12022.1; -. [Q9BYG4-1]
DR RefSeq; NP_115899.1; NM_032510.3. [Q9BYG4-1]
DR AlphaFoldDB; Q9BYG4; -.
DR SMR; Q9BYG4; -.
DR BioGRID; 124135; 40.
DR ComplexPortal; CPX-6194; PAR cell polarity complex, PARD6G-PRKCI variant.
DR ComplexPortal; CPX-6195; PAR cell polarity complex, PARD6G-PRKCZ variant.
DR IntAct; Q9BYG4; 16.
DR MINT; Q9BYG4; -.
DR STRING; 9606.ENSP00000343144; -.
DR iPTMnet; Q9BYG4; -.
DR PhosphoSitePlus; Q9BYG4; -.
DR BioMuta; PARD6G; -.
DR DMDM; 30913175; -.
DR EPD; Q9BYG4; -.
DR jPOST; Q9BYG4; -.
DR MassIVE; Q9BYG4; -.
DR MaxQB; Q9BYG4; -.
DR PaxDb; Q9BYG4; -.
DR PeptideAtlas; Q9BYG4; -.
DR PRIDE; Q9BYG4; -.
DR ProteomicsDB; 79638; -. [Q9BYG4-1]
DR Antibodypedia; 23537; 108 antibodies from 19 providers.
DR DNASU; 84552; -.
DR Ensembl; ENST00000353265.8; ENSP00000343144.3; ENSG00000178184.16. [Q9BYG4-1]
DR Ensembl; ENST00000470488.2; ENSP00000468735.1; ENSG00000178184.16. [Q9BYG4-2]
DR GeneID; 84552; -.
DR KEGG; hsa:84552; -.
DR MANE-Select; ENST00000353265.8; ENSP00000343144.3; NM_032510.4; NP_115899.1.
DR UCSC; uc002lny.4; human. [Q9BYG4-1]
DR CTD; 84552; -.
DR DisGeNET; 84552; -.
DR GeneCards; PARD6G; -.
DR HGNC; HGNC:16076; PARD6G.
DR HPA; ENSG00000178184; Tissue enhanced (skin).
DR MIM; 608976; gene.
DR neXtProt; NX_Q9BYG4; -.
DR OpenTargets; ENSG00000178184; -.
DR PharmGKB; PA32939; -.
DR VEuPathDB; HostDB:ENSG00000178184; -.
DR eggNOG; KOG3606; Eukaryota.
DR GeneTree; ENSGT00950000183211; -.
DR HOGENOM; CLU_040653_2_0_1; -.
DR InParanoid; Q9BYG4; -.
DR OMA; HTNTSDE; -.
DR OrthoDB; 825211at2759; -.
DR PhylomeDB; Q9BYG4; -.
DR TreeFam; TF312899; -.
DR PathwayCommons; Q9BYG4; -.
DR Reactome; R-HSA-420029; Tight junction interactions.
DR SignaLink; Q9BYG4; -.
DR BioGRID-ORCS; 84552; 9 hits in 1074 CRISPR screens.
DR ChiTaRS; PARD6G; human.
DR GenomeRNAi; 84552; -.
DR Pharos; Q9BYG4; Tbio.
DR PRO; PR:Q9BYG4; -.
DR Proteomes; UP000005640; Chromosome 18.
DR RNAct; Q9BYG4; protein.
DR Bgee; ENSG00000178184; Expressed in upper arm skin and 157 other tissues.
DR ExpressionAtlas; Q9BYG4; baseline and differential.
DR Genevisible; Q9BYG4; HS.
DR GO; GO:0016324; C:apical plasma membrane; IBA:GO_Central.
DR GO; GO:0005923; C:bicellular tight junction; IEA:UniProtKB-SubCell.
DR GO; GO:0005938; C:cell cortex; IBA:GO_Central.
DR GO; GO:0005829; C:cytosol; TAS:Reactome.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0005886; C:plasma membrane; TAS:Reactome.
DR GO; GO:0070160; C:tight junction; IC:ComplexPortal.
DR GO; GO:0005080; F:protein kinase C binding; IBA:GO_Central.
DR GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
DR GO; GO:0007098; P:centrosome cycle; IBA:GO_Central.
DR GO; GO:0007163; P:establishment or maintenance of cell polarity; IBA:GO_Central.
DR GO; GO:0060341; P:regulation of cellular localization; IBA:GO_Central.
DR CDD; cd06403; PB1_Par6; 1.
DR Gene3D; 2.30.42.10; -; 1.
DR InterPro; IPR034874; PAR-6_G.
DR InterPro; IPR000270; PB1_dom.
DR InterPro; IPR034868; PB1_Par6.
DR InterPro; IPR001478; PDZ.
DR InterPro; IPR036034; PDZ_sf.
DR PANTHER; PTHR14102:SF3; PTHR14102:SF3; 1.
DR Pfam; PF00564; PB1; 1.
DR Pfam; PF00595; PDZ; 1.
DR SMART; SM00666; PB1; 1.
DR SMART; SM00228; PDZ; 1.
DR SUPFAM; SSF50156; SSF50156; 1.
DR PROSITE; PS51745; PB1; 1.
DR PROSITE; PS50106; PDZ; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Cell cycle; Cell division; Cell junction;
KW Cell membrane; Cytoplasm; Membrane; Reference proteome; Tight junction.
FT CHAIN 1..376
FT /note="Partitioning defective 6 homolog gamma"
FT /id="PRO_0000112518"
FT DOMAIN 18..98
FT /note="PB1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01081"
FT DOMAIN 134..151
FT /note="Pseudo-CRIB"
FT DOMAIN 158..251
FT /note="PDZ"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00143"
FT REGION 127..254
FT /note="Interaction with PARD3 and CDC42"
FT /evidence="ECO:0000250"
FT REGION 356..376
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 99..107
FT /note="EEAERGSLG -> DDGALRPGT (in isoform 2)"
FT /evidence="ECO:0000303|Ref.2"
FT /id="VSP_047586"
FT VAR_SEQ 108..376
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|Ref.2"
FT /id="VSP_047587"
SQ SEQUENCE 376 AA; 40883 MW; E5A4EF521403CA11 CRC64;
MNRSFHKSQT LRFYDCSAVE VKSKFGAEFR RFSLDRHKPG KFEDFYKLVV HTHHISNSDV
TIGYADVHGD LLPINNDDNF CKAVSSANPL LRVFIQKREE AERGSLGAGS LCRRRRALGA
LRDEGPRRRA HLDIGLPRDF RPVSSIIDVD LVPETHRRVR LHRHGCEKPL GFYIRDGASV
RVTPHGLEKV PGIFISRMVP GGLAESTGLL AVNDEVLEVN GIEVAGKTLD QVTDMMIANS
HNLIVTVKPA NQRNNVVRGG RALGSSGPPS DGTAGFVGPP APRVLQNFHP DEAESDEDND
VVIEGTLEPA RPPQTPGAPA GSLSRVNGAG LAQRLQRDLA LDGGLQRLLS SLRADPRHSL
ALPPGGVEEH GPAVTL
