ID   PARA4_ECOLX             Reviewed;         219 AA.
AC   P22996;
DT   01-AUG-1991, integrated into UniProtKB/Swiss-Prot.
DT   01-AUG-1991, sequence version 1.
DT   25-MAY-2022, entry version 82.
DE   RecName: Full=Resolvase;
GN   Name=parA;
OS   Escherichia coli.
OG   Plasmid IncP-alpha RP4.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=562;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=2172207; DOI=10.1128/jb.172.11.6194-6203.1990;
RA   Gerlitz M., Hrabak O., Schwab H.;
RT   "Partitioning of broad-host-range plasmid RP4 is a complex system involving
RT   site-specific recombination.";
RL   J. Bacteriol. 172:6194-6203(1990).
CC   -!- FUNCTION: Involved in plasmid partition.
CC   -!- SIMILARITY: Belongs to the site-specific recombinase resolvase family.
CC       {ECO:0000305}.
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DR   EMBL; M59825; AAA26414.1; -; Genomic_DNA.
DR   PIR; D37141; D37141.
DR   RefSeq; WP_011205802.1; NZ_VKJF01000079.1.
DR   AlphaFoldDB; P22996; -.
DR   SMR; P22996; -.
DR   PRIDE; P22996; -.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0000150; F:DNA strand exchange activity; IEA:InterPro.
DR   GO; GO:0015074; P:DNA integration; IEA:UniProtKB-KW.
DR   GO; GO:0030541; P:plasmid partitioning; IEA:UniProtKB-KW.
DR   Gene3D; 3.40.50.1390; -; 1.
DR   InterPro; IPR006118; Recombinase_CS.
DR   InterPro; IPR006119; Resolv_N.
DR   InterPro; IPR036162; Resolvase-like_N_sf.
DR   InterPro; IPR006120; Resolvase_HTH_dom.
DR   Pfam; PF02796; HTH_7; 1.
DR   Pfam; PF00239; Resolvase; 1.
DR   SMART; SM00857; Resolvase; 1.
DR   SUPFAM; SSF53041; SSF53041; 1.
DR   PROSITE; PS00397; RECOMBINASES_1; 1.
DR   PROSITE; PS00398; RECOMBINASES_2; 1.
DR   PROSITE; PS51736; RECOMBINASES_3; 1.
PE   3: Inferred from homology;
KW   DNA integration; DNA recombination; DNA-binding; Plasmid;
KW   Plasmid partition.
FT   CHAIN           1..219
FT                   /note="Resolvase"
FT                   /id="PRO_0000196384"
FT   DOMAIN          15..159
FT                   /note="Resolvase/invertase-type recombinase catalytic"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01072"
FT   ACT_SITE        23
FT                   /note="O-(5'-phospho-DNA)-serine intermediate"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01072"
SQ   SEQUENCE   219 AA;  24161 MW;  E19BB0C5D4C1E58C CRC64;
     MATREQQPEG RRLKVARIYL RASTDEQNLE RQESLVAATR AAGYYVAGIY REKASGARAD
     RPELLRMIAD LQPGEVVVAE KIDRISRLPL AEAERLVASI RAKGAKLAVP GVVDLSELAA
     EANGVAKIVL ESVQDMLLKL ALQMARDDYE DRRERQRQGV QLAKAAGRYT GRKRDAGMHD
     RIITLRSGGS SIAKTAKLVG CSPSQVKRVW AAWNAQQQK