ID   A36_VACCC               Reviewed;         221 AA.
AC   P68618; P21059;
DT   07-DEC-2004, integrated into UniProtKB/Swiss-Prot.
DT   07-DEC-2004, sequence version 1.
DT   29-SEP-2021, entry version 42.
DE   RecName: Full=Protein A36;
GN   ORFNames=A36R;
OS   Vaccinia virus (strain Copenhagen) (VACV).
OC   Viruses; Varidnaviria; Bamfordvirae; Nucleocytoviricota; Pokkesviricetes;
OC   Chitovirales; Poxviridae; Chordopoxvirinae; Orthopoxvirus; Vaccinia virus.
OX   NCBI_TaxID=10249;
OH   NCBI_TaxID=9606; Homo sapiens (Human).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=2219722; DOI=10.1016/0042-6822(90)90294-2;
RA   Goebel S.J., Johnson G.P., Perkus M.E., Davis S.W., Winslow J.P.,
RA   Paoletti E.;
RT   "The complete DNA sequence of vaccinia virus.";
RL   Virology 179:247-266(1990).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA   Goebel S.J., Johnson G.P., Perkus M.E., Davis S.W., Winslow J.P.,
RA   Paoletti E.;
RT   "Appendix to 'The complete DNA sequence of vaccinia virus'.";
RL   Virology 179:517-563(1990).
CC   -!- FUNCTION: Involved in the intracellular transport of virions to the
CC       host cell surface. Participates also in the formation of actin tails at
CC       the plasma membrane to allow efficient actin-based motility and thus
CC       cell to cell transmission of viral particles. Mechanistically,
CC       phosphorylation of Tyr-112 and 132 of A36 activates the host ARP2-ARP3
CC       complex and leads to actin nucleation (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Interacts with host NCK. Interacts with protein A33 (via C-
CC       terminus). Interacts with protein F12. Interacts (via C-terminus) with
CC       host kinesin light chain 1 (KLC1) (By similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Host membrane {ECO:0000305}; Single-pass membrane
CC       protein {ECO:0000305}. Note=Found exclusively on the wrapped enveloped
CC       virion. Absent in the mature virion (MV) and extracellular enveloped
CC       virion (EV) (By similarity). {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the orthopoxvirus A36 protein family.
CC       {ECO:0000305}.
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DR   EMBL; M35027; AAA48164.1; -; Genomic_DNA.
DR   PIR; JQ1772; B42521.
DR   Proteomes; UP000008269; Genome.
DR   GO; GO:0033644; C:host cell membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   InterPro; IPR010274; Orthopox_A36R.
DR   Pfam; PF05950; Orthopox_A36R; 1.
PE   3: Inferred from homology;
KW   Host membrane; Host-virus interaction; Membrane; Phosphoprotein;
KW   Reference proteome; Transmembrane; Transmembrane helix.
FT   CHAIN           1..221
FT                   /note="Protein A36"
FT                   /id="PRO_0000040599"
FT   TOPO_DOM        1
FT                   /note="Extracellular"
FT   TRANSMEM        2..22
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        23..221
FT                   /note="Cytoplasmic"
FT   MOD_RES         112
FT                   /note="Phosphotyrosine; by host"
FT                   /evidence="ECO:0000250"
FT   MOD_RES         132
FT                   /note="Phosphotyrosine; by host"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   221 AA;  25133 MW;  5D15758981B0E2E2 CRC64;
     MMLVPLITVT VVAGTILVCY ILYICRKKIR TVYNDNKIIM TKLKKIKSSN SSKSSKSTDS
     ESDWEDHCSA MEQNNDVDNI SRNEILDDDS FAGSLIWDNE SNVMAPSTEH IYDSVAGSTL
     LINNDRNEQT IYQNTTVVIN ETETVEVLNE DTKQNPNYSS NPFVNYNKTS ICSKSNPFIT
     ELNNKFSENN PFRRAHSDDY LNKQEQDHEH DDIESSVVSL V