ID   PARB_TRYBB              Reviewed;         129 AA.
AC   P09791;
DT   01-JUL-1989, integrated into UniProtKB/Swiss-Prot.
DT   01-JUL-1989, sequence version 1.
DT   25-MAY-2022, entry version 79.
DE   RecName: Full=Procyclic form-specific polypeptide A-beta;
DE   AltName: Full=PARP A-beta;
DE   AltName: Full=Procyclin;
DE   Flags: Precursor;
GN   Name=PARPA-BETA;
OS   Trypanosoma brucei brucei.
OC   Eukaryota; Discoba; Euglenozoa; Kinetoplastea; Metakinetoplastina;
OC   Trypanosomatida; Trypanosomatidae; Trypanosoma.
OX   NCBI_TaxID=5702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=427;
RX   PubMed=3808022; DOI=10.1038/325272a0;
RA   Roditi I., Carrington M., Turner M.;
RT   "Expression of a polypeptide containing a dipeptide repeat is confined to
RT   the insect stage of Trypanosoma brucei.";
RL   Nature 325:272-274(1987).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=2342468; DOI=10.1128/mcb.10.6.3036-3047.1990;
RA   Clayton C.E., Fueri J.P., Itzhaki J.E., Bellofatto V., Sherman D.R.,
RA   Wisdom G.S., Vijayasarathy S., Mowatt M.R.;
RT   "Transcription of the procyclic acidic repetitive protein genes of
RT   Trypanosoma brucei.";
RL   Mol. Cell. Biol. 10:3036-3047(1990).
RN   [3]
RP   NUCLEOTIDE SEQUENCE.
RC   STRAIN=427;
RA   Vijayasarathy S., Ernest I., Itzhaki J., Sherman D., Mowatt M.R.,
RA   Michels P.A.M., Clayton C.E.;
RL   Submitted (JUN-1992) to the EMBL/GenBank/DDBJ databases.
RN   [4]
RP   PROTEIN SEQUENCE OF 28-64, GPI-ANCHOR AT GLY-107, AND GLYCOSYLATION AT
RP   ASN-56.
RC   STRAIN=427;
RX   PubMed=2475493; DOI=10.1016/s0021-9258(18)63815-3;
RA   Clayton C.E., Mowatt M.R.;
RT   "The procyclic acidic repetitive proteins of Trypanosoma brucei.
RT   Purification and post-translational modification.";
RL   J. Biol. Chem. 264:15088-15093(1989).
CC   -!- FUNCTION: Major surface antigen of procyclic forms.
CC   -!- SUBCELLULAR LOCATION: Cell membrane; Lipid-anchor, GPI-anchor.
CC   -!- DEVELOPMENTAL STAGE: Expressed only at a certain stage during
CC       differentiation in the insect vector.
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DR   EMBL; X04814; CAA28503.1; -; mRNA.
DR   EMBL; M33129; AAA30225.1; -; Genomic_DNA.
DR   EMBL; X52584; CAA36815.1; -; Genomic_DNA.
DR   PIR; A26036; A26036.
DR   AlphaFoldDB; P09791; -.
DR   iPTMnet; P09791; -.
DR   GO; GO:0031225; C:anchored component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   InterPro; IPR008882; Trypano_PARP.
DR   Pfam; PF05887; Trypan_PARP; 1.
PE   1: Evidence at protein level;
KW   Cell membrane; Direct protein sequencing; Glycoprotein; GPI-anchor;
KW   Lipoprotein; Membrane; Repeat; Signal.
FT   SIGNAL          1..27
FT                   /evidence="ECO:0000269|PubMed:2475493"
FT   CHAIN           28..107
FT                   /note="Procyclic form-specific polypeptide A-beta"
FT                   /id="PRO_0000022013"
FT   PROPEP          108..129
FT                   /id="PRO_0000022014"
FT   REPEAT          59..60
FT                   /note="1"
FT   REPEAT          61..62
FT                   /note="2"
FT   REPEAT          63..64
FT                   /note="3"
FT   REPEAT          65..66
FT                   /note="4"
FT   REPEAT          67..68
FT                   /note="5"
FT   REPEAT          69..70
FT                   /note="6"
FT   REPEAT          71..72
FT                   /note="7"
FT   REPEAT          73..74
FT                   /note="8"
FT   REPEAT          75..76
FT                   /note="9"
FT   REPEAT          77..78
FT                   /note="10"
FT   REPEAT          79..80
FT                   /note="11"
FT   REPEAT          81..82
FT                   /note="12"
FT   REPEAT          83..84
FT                   /note="13"
FT   REPEAT          85..86
FT                   /note="14"
FT   REPEAT          87..88
FT                   /note="15"
FT   REPEAT          89..90
FT                   /note="16"
FT   REPEAT          91..92
FT                   /note="17"
FT   REPEAT          93..94
FT                   /note="18"
FT   REPEAT          95..96
FT                   /note="19"
FT   REPEAT          97..98
FT                   /note="20"
FT   REPEAT          99..100
FT                   /note="21"
FT   REPEAT          101..102
FT                   /note="22"
FT   REPEAT          103..104
FT                   /note="23"
FT   REPEAT          105..106
FT                   /note="24"
FT   REGION          27..111
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          59..106
FT                   /note="24 X 2 AA tandem repeats of [DE]-P"
FT   COMPBIAS        34..56
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        57..102
FT                   /note="Acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   LIPID           107
FT                   /note="GPI-anchor amidated glycine"
FT                   /evidence="ECO:0000269|PubMed:2475493"
FT   CARBOHYD        56
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000269|PubMed:2475493"
SQ   SEQUENCE   129 AA;  13314 MW;  063A1E64D8718E86 CRC64;
     MAPRSLYLLA VLLFSANLFA GVGFAAAAEG PEDKGLTKGG KGKGGKGTKV SDDDTNGTDP
     DPEPEPEPEP EPEPEPEPEP EPEPEPEPEP EPEPEPEPEP EPEPEPGAAT LKSVALPFAI
     AAVGLVAAF