ID   PARC_CROSK              Reviewed;         110 AA.
AC   O68891;
DT   15-DEC-1998, integrated into UniProtKB/Swiss-Prot.
DT   01-AUG-1998, sequence version 1.
DT   25-MAY-2022, entry version 82.
DE   RecName: Full=DNA topoisomerase 4 subunit A;
DE            EC=5.6.2.2;
DE   AltName: Full=Topoisomerase IV subunit A;
DE   Flags: Fragment;
GN   Name=parC;
OS   Cronobacter sakazakii (Enterobacter sakazakii).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Cronobacter.
OX   NCBI_TaxID=28141;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 12868 / NCIMB 5920 / NCTC 5920 / 502;
RA   Pirapatrungsuriya K., Amyes S.G.B., Thomson C.J.;
RL   Submitted (MAR-1998) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Topoisomerase IV is essential for chromosome segregation. It
CC       relaxes supercoiled DNA. Performs the decatenation events required
CC       during the replication of a circular DNA molecule (By similarity).
CC       {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP-dependent breakage, passage and rejoining of double-
CC         stranded DNA.; EC=5.6.2.2;
CC   -!- SUBUNIT: Heterotetramer composed of ParC and ParE. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000250}; Peripheral membrane
CC       protein {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the type II topoisomerase GyrA/ParC subunit
CC       family. ParC type 1 subfamily. {ECO:0000305}.
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DR   EMBL; AF056287; AAC13494.1; -; Genomic_DNA.
DR   AlphaFoldDB; O68891; -.
DR   SMR; O68891; -.
DR   STRING; 28141.CSK29544_01645; -.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0005524; F:ATP binding; IEA:InterPro.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0003918; F:DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) activity; IEA:UniProtKB-EC.
DR   GO; GO:0006265; P:DNA topological change; IEA:InterPro.
DR   Gene3D; 3.90.199.10; -; 1.
DR   InterPro; IPR013760; Topo_IIA-like_dom_sf.
DR   InterPro; IPR013758; Topo_IIA_A/C_ab.
DR   InterPro; IPR002205; Topo_IIA_dom_A.
DR   Pfam; PF00521; DNA_topoisoIV; 1.
DR   SMART; SM00434; TOP4c; 1.
DR   SUPFAM; SSF56719; SSF56719; 1.
PE   3: Inferred from homology;
KW   Cell membrane; DNA-binding; Isomerase; Membrane; Topoisomerase.
FT   CHAIN           1..>110
FT                   /note="DNA topoisomerase 4 subunit A"
FT                   /id="PRO_0000145399"
FT   ACT_SITE        98
FT                   /note="O-(5'-phospho-DNA)-tyrosine intermediate"
FT                   /evidence="ECO:0000250"
FT   SITE            17
FT                   /note="Interaction with DNA"
FT                   /evidence="ECO:0000250"
FT   SITE            53
FT                   /note="Interaction with DNA"
FT                   /evidence="ECO:0000250"
FT   SITE            55
FT                   /note="Interaction with DNA"
FT                   /evidence="ECO:0000250"
FT   SITE            97
FT                   /note="Transition state stabilizer"
FT                   /evidence="ECO:0000250"
FT   NON_TER         110
SQ   SEQUENCE   110 AA;  12314 MW;  EFD87D7ED5ECBA35 CRC64;
     MYVIMDRALP FIGDGLKPVQ RRIVYAMSEL GLNASAKFKK SARTVGDVLG KYHPHGDSAC
     YEAMVLMAQP FSYRYPLVDG QGNWGAPDDP KSFAAMRYTE SRLSKYSELL