PARC_PSEAE
ID PARC_PSEAE Reviewed; 754 AA.
AC Q9HUK1;
DT 15-MAY-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2001, sequence version 1.
DT 03-AUG-2022, entry version 132.
DE RecName: Full=DNA topoisomerase 4 subunit A {ECO:0000255|HAMAP-Rule:MF_00936};
DE EC=5.6.2.2 {ECO:0000255|HAMAP-Rule:MF_00936};
DE AltName: Full=Topoisomerase IV subunit A {ECO:0000255|HAMAP-Rule:MF_00936};
GN Name=parC {ECO:0000255|HAMAP-Rule:MF_00936}; OrderedLocusNames=PA4964;
OS Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM
OS 14847 / LMG 12228 / 1C / PRS 101 / PAO1).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC Pseudomonadaceae; Pseudomonas.
OX NCBI_TaxID=208964;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C /
RC PRS 101 / PAO1;
RX PubMed=10984043; DOI=10.1038/35023079;
RA Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P.,
RA Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M.,
RA Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y.,
RA Brody L.L., Coulter S.N., Folger K.R., Kas A., Larbig K., Lim R.M.,
RA Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J.,
RA Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V.;
RT "Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic
RT pathogen.";
RL Nature 406:959-964(2000).
CC -!- FUNCTION: Topoisomerase IV is essential for chromosome segregation. It
CC relaxes supercoiled DNA. Performs the decatenation events required
CC during the replication of a circular DNA molecule. {ECO:0000255|HAMAP-
CC Rule:MF_00936}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP-dependent breakage, passage and rejoining of double-
CC stranded DNA.; EC=5.6.2.2; Evidence={ECO:0000255|HAMAP-
CC Rule:MF_00936};
CC -!- SUBUNIT: Heterotetramer composed of ParC and ParE. {ECO:0000255|HAMAP-
CC Rule:MF_00936}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000255|HAMAP-Rule:MF_00936};
CC Peripheral membrane protein {ECO:0000255|HAMAP-Rule:MF_00936}.
CC -!- SIMILARITY: Belongs to the type II topoisomerase GyrA/ParC subunit
CC family. ParC type 1 subfamily. {ECO:0000255|HAMAP-Rule:MF_00936}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AE004091; AAG08349.1; -; Genomic_DNA.
DR PIR; G83025; G83025.
DR RefSeq; NP_253651.1; NC_002516.2.
DR RefSeq; WP_003095687.1; NZ_QZGE01000002.1.
DR AlphaFoldDB; Q9HUK1; -.
DR SMR; Q9HUK1; -.
DR STRING; 287.DR97_2317; -.
DR ChEMBL; CHEMBL3390827; -.
DR PaxDb; Q9HUK1; -.
DR PRIDE; Q9HUK1; -.
DR EnsemblBacteria; AAG08349; AAG08349; PA4964.
DR GeneID; 879741; -.
DR KEGG; pae:PA4964; -.
DR PATRIC; fig|208964.12.peg.5198; -.
DR PseudoCAP; PA4964; -.
DR HOGENOM; CLU_002977_4_1_6; -.
DR InParanoid; Q9HUK1; -.
DR OMA; PRSNRID; -.
DR PhylomeDB; Q9HUK1; -.
DR BioCyc; PAER208964:G1FZ6-5080-MON; -.
DR Proteomes; UP000002438; Chromosome.
DR GO; GO:0005694; C:chromosome; IEA:InterPro.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0009340; C:DNA topoisomerase IV complex; IDA:PseudoCAP.
DR GO; GO:0009330; C:DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) complex; IBA:GO_Central.
DR GO; GO:0019897; C:extrinsic component of plasma membrane; IEA:UniProtKB-UniRule.
DR GO; GO:0005524; F:ATP binding; IBA:GO_Central.
DR GO; GO:0003677; F:DNA binding; IBA:GO_Central.
DR GO; GO:0003918; F:DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) activity; IDA:PseudoCAP.
DR GO; GO:0007059; P:chromosome segregation; IBA:GO_Central.
DR GO; GO:0006265; P:DNA topological change; IDA:PseudoCAP.
DR CDD; cd00187; TOP4c; 1.
DR Gene3D; 1.10.268.10; -; 1.
DR Gene3D; 2.120.10.90; -; 1.
DR Gene3D; 3.90.199.10; -; 1.
DR HAMAP; MF_00936; ParC_type1; 1.
DR InterPro; IPR006691; GyrA/parC_rep.
DR InterPro; IPR035516; Gyrase/topoIV_suA_C.
DR InterPro; IPR013760; Topo_IIA-like_dom_sf.
DR InterPro; IPR013758; Topo_IIA_A/C_ab.
DR InterPro; IPR013757; Topo_IIA_A_a_sf.
DR InterPro; IPR002205; Topo_IIA_dom_A.
DR InterPro; IPR005742; TopoIV_A_Gneg.
DR Pfam; PF03989; DNA_gyraseA_C; 2.
DR Pfam; PF00521; DNA_topoisoIV; 1.
DR SMART; SM00434; TOP4c; 1.
DR SUPFAM; SSF101904; SSF101904; 1.
DR SUPFAM; SSF56719; SSF56719; 1.
PE 3: Inferred from homology;
KW Cell membrane; DNA-binding; Isomerase; Membrane; Reference proteome;
KW Topoisomerase.
FT CHAIN 1..754
FT /note="DNA topoisomerase 4 subunit A"
FT /id="PRO_0000287821"
FT ACT_SITE 127
FT /note="O-(5'-phospho-DNA)-tyrosine intermediate"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00936"
FT SITE 46
FT /note="Interaction with DNA"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00936"
FT SITE 82
FT /note="Interaction with DNA"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00936"
FT SITE 84
FT /note="Interaction with DNA"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00936"
FT SITE 126
FT /note="Transition state stabilizer"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00936"
SQ SEQUENCE 754 AA; 83365 MW; D642F8FF157E573C CRC64;
MSESLDLSLE GVERRSLAEF TEQAYLNYSM YVIMDRALPH IGDGLKPVQR RIVYAMSELG
LDADSKHKKS ARTVGDVLGK FHPHGDSACY EAMVLMAQPF SYRYPLVDGQ GNWGAPDDPK
SFAAMRYTEA RLSRYSEVLL SELGQGTVDW VPNFDGTLDE PAVLPARLPN LLLNGTTGIA
VGMATDVPPH NLREVASACV RLLDQPGATV AELCEHVPGP DFPTEAEIIT PRADLQKVYE
TGRGSVRMRA VYRVEDGDIV IHALPHQVSG SKVLEQIAGQ MQAKKLPMVA DLRDESDHEN
PTRIVIIPRS NRVDVEELMT HLFATTDLET SYRVNLNIIG LDGKPQVKDL RQLLSEWLQF
RIGTVRRRLQ FRLDKVERRL HLLDGLLIAF LNLDEVIHII RTEDQPKAVL MERFELSEVQ
ADYILDTRLR QLARLEEMKI RGEQEELLKE QKRLQTLLGS EAKLKKLVRE ELIKDAETYG
DDRRSPIVAR AEARALSETE LMPTEPVTVV LSEKGWVRCA KGHDIDAAGL SYKAGDGFKA
AAPGRSNQYA VFIDSTGRSY SLPAHSLPSA RGQGEPLSGR LTPPPGASFE CVLLPDDDAL
FVIASDAGYG FVVKGEDLQA KNKAGKALLS LPNGSAVVAP RPVRDVEQDW LAAVTTEGRL
LLFKVSDLPQ LGKGKGNKII GIPGERVASR EEYLTDLAVL PAGATLVLQA GKRTLSLKGD
DLEHYKGERG RRGNKLPRGF QRVDSLLVDI PPQD
