ID   PARC_RHIME              Reviewed;         758 AA.
AC   Q59749;
DT   30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT   30-MAY-2000, sequence version 2.
DT   03-AUG-2022, entry version 137.
DE   RecName: Full=DNA topoisomerase 4 subunit A {ECO:0000255|HAMAP-Rule:MF_00936};
DE            EC=5.6.2.2 {ECO:0000255|HAMAP-Rule:MF_00936};
DE   AltName: Full=Topoisomerase IV subunit A {ECO:0000255|HAMAP-Rule:MF_00936};
GN   Name=parC {ECO:0000255|HAMAP-Rule:MF_00936}; OrderedLocusNames=R01199;
GN   ORFNames=SMc01761;
OS   Rhizobium meliloti (strain 1021) (Ensifer meliloti) (Sinorhizobium
OS   meliloti).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC   Rhizobiaceae; Sinorhizobium/Ensifer group; Sinorhizobium.
OX   NCBI_TaxID=266834;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=1021;
RX   PubMed=11481430; DOI=10.1073/pnas.161294398;
RA   Capela D., Barloy-Hubler F., Gouzy J., Bothe G., Ampe F., Batut J.,
RA   Boistard P., Becker A., Boutry M., Cadieu E., Dreano S., Gloux S.,
RA   Godrie T., Goffeau A., Kahn D., Kiss E., Lelaure V., Masuy D., Pohl T.,
RA   Portetelle D., Puehler A., Purnelle B., Ramsperger U., Renard C.,
RA   Thebault P., Vandenbol M., Weidner S., Galibert F.;
RT   "Analysis of the chromosome sequence of the legume symbiont Sinorhizobium
RT   meliloti strain 1021.";
RL   Proc. Natl. Acad. Sci. U.S.A. 98:9877-9882(2001).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=1021;
RX   PubMed=11474104; DOI=10.1126/science.1060966;
RA   Galibert F., Finan T.M., Long S.R., Puehler A., Abola P., Ampe F.,
RA   Barloy-Hubler F., Barnett M.J., Becker A., Boistard P., Bothe G.,
RA   Boutry M., Bowser L., Buhrmester J., Cadieu E., Capela D., Chain P.,
RA   Cowie A., Davis R.W., Dreano S., Federspiel N.A., Fisher R.F., Gloux S.,
RA   Godrie T., Goffeau A., Golding B., Gouzy J., Gurjal M., Hernandez-Lucas I.,
RA   Hong A., Huizar L., Hyman R.W., Jones T., Kahn D., Kahn M.L., Kalman S.,
RA   Keating D.H., Kiss E., Komp C., Lelaure V., Masuy D., Palm C., Peck M.C.,
RA   Pohl T.M., Portetelle D., Purnelle B., Ramsperger U., Surzycki R.,
RA   Thebault P., Vandenbol M., Vorhoelter F.J., Weidner S., Wells D.H.,
RA   Wong K., Yeh K.-C., Batut J.;
RT   "The composite genome of the legume symbiont Sinorhizobium meliloti.";
RL   Science 293:668-672(2001).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 45-192.
RC   STRAIN=RCR2011 / SU47;
RA   Rosanas A., Barb J., Gibert I.;
RL   Submitted (FEB-1996) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Topoisomerase IV is essential for chromosome segregation. It
CC       relaxes supercoiled DNA. Performs the decatenation events required
CC       during the replication of a circular DNA molecule. {ECO:0000255|HAMAP-
CC       Rule:MF_00936}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP-dependent breakage, passage and rejoining of double-
CC         stranded DNA.; EC=5.6.2.2; Evidence={ECO:0000255|HAMAP-
CC         Rule:MF_00936};
CC   -!- SUBUNIT: Heterotetramer composed of ParC and ParE. {ECO:0000255|HAMAP-
CC       Rule:MF_00936}.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000255|HAMAP-Rule:MF_00936};
CC       Peripheral membrane protein {ECO:0000255|HAMAP-Rule:MF_00936}.
CC   -!- SIMILARITY: Belongs to the type II topoisomerase GyrA/ParC subunit
CC       family. ParC type 1 subfamily. {ECO:0000255|HAMAP-Rule:MF_00936}.
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DR   EMBL; AL591688; CAC45778.1; -; Genomic_DNA.
DR   EMBL; X95674; CAA64977.1; -; Genomic_DNA.
DR   RefSeq; NP_385305.1; NC_003047.1.
DR   RefSeq; WP_003529331.1; NC_003047.1.
DR   AlphaFoldDB; Q59749; -.
DR   SMR; Q59749; -.
DR   STRING; 266834.SMc01761; -.
DR   PRIDE; Q59749; -.
DR   EnsemblBacteria; CAC45778; CAC45778; SMc01761.
DR   GeneID; 61602657; -.
DR   KEGG; sme:SMc01761; -.
DR   PATRIC; fig|266834.11.peg.2610; -.
DR   eggNOG; COG0188; Bacteria.
DR   HOGENOM; CLU_002977_4_1_5; -.
DR   OMA; PRSNRID; -.
DR   Proteomes; UP000001976; Chromosome.
DR   GO; GO:0005694; C:chromosome; IEA:InterPro.
DR   GO; GO:0019897; C:extrinsic component of plasma membrane; IEA:UniProtKB-UniRule.
DR   GO; GO:0005524; F:ATP binding; IEA:InterPro.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0003918; F:DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0007059; P:chromosome segregation; IEA:UniProtKB-UniRule.
DR   GO; GO:0006265; P:DNA topological change; IEA:UniProtKB-UniRule.
DR   CDD; cd00187; TOP4c; 1.
DR   Gene3D; 1.10.268.10; -; 1.
DR   Gene3D; 2.120.10.90; -; 1.
DR   Gene3D; 3.90.199.10; -; 1.
DR   HAMAP; MF_00936; ParC_type1; 1.
DR   InterPro; IPR006691; GyrA/parC_rep.
DR   InterPro; IPR035516; Gyrase/topoIV_suA_C.
DR   InterPro; IPR013760; Topo_IIA-like_dom_sf.
DR   InterPro; IPR013758; Topo_IIA_A/C_ab.
DR   InterPro; IPR013757; Topo_IIA_A_a_sf.
DR   InterPro; IPR002205; Topo_IIA_dom_A.
DR   InterPro; IPR005742; TopoIV_A_Gneg.
DR   Pfam; PF03989; DNA_gyraseA_C; 3.
DR   Pfam; PF00521; DNA_topoisoIV; 1.
DR   SMART; SM00434; TOP4c; 1.
DR   SUPFAM; SSF101904; SSF101904; 1.
DR   SUPFAM; SSF56719; SSF56719; 1.
PE   3: Inferred from homology;
KW   Cell membrane; DNA-binding; Isomerase; Membrane; Reference proteome;
KW   Topoisomerase.
FT   CHAIN           1..758
FT                   /note="DNA topoisomerase 4 subunit A"
FT                   /id="PRO_0000145404"
FT   ACT_SITE        129
FT                   /note="O-(5'-phospho-DNA)-tyrosine intermediate"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00936"
FT   SITE            49
FT                   /note="Interaction with DNA"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00936"
FT   SITE            85
FT                   /note="Interaction with DNA"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00936"
FT   SITE            87
FT                   /note="Interaction with DNA"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00936"
FT   SITE            128
FT                   /note="Transition state stabilizer"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00936"
FT   CONFLICT        145..147
FT                   /note="IDQ -> STL (in Ref. 3; CAA64977)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        188
FT                   /note="S -> N (in Ref. 3; CAA64977)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   758 AA;  83754 MW;  D2AF7AF65EFA1329 CRC64;
     MGQSLLPPSG GDDNIQPVDL KAALEERYLA YALSTIMHRA LPDVRDGLKP VHRRIIHAMS
     EMGLRPNSSF KKCARIVGDV IGKFHPHGDQ SVYDALVRLA QDFSQRYPVV DGQGNFGNID
     GDNAAAYRYT EAKMTEVAAL LLEGIDQDAV DFRPTYNEED QEPTVLPGAF PNLLANGASG
     IAVGMATSIP PHNAHELCDA ALHLIKHPDA TVEDLLFDPA NPQRGGIEGP DFPTGGVIVE
     SRASMAESYR TGRGGFRVRA RWAVEDLGRG GFQIVVTEIP YQVQKSRLIE KIAELLIARK
     LPLLEDIRDE SAEDVRVVLV PKSRSVDANI LMESLFKLTE LESRIPLNMN VLSMGRVPRV
     MALNEVLSEW LAHRREVLQR RSRHRLAAID RRLEILGGYL IAYLNIDEVI RIIREEDEPK
     AVMIERFGLT DVQAEAILNM RLRSLRKLEE FEIRTEFDSL SKEKAEIEAL LASGDKQWQA
     VAWEIGEVKK KFAKATELGK RRSTFSDAPD ADVEAIQQAM IEKEPITVVI SEKGWIRALK
     GHIADTSSLQ FKDGDGLKVS FPAQTTDKIL IFTTGGKAYT LGGDKLPGGR GHGEPLRIMV
     DMENDQDVLT AFVHDPARKL IVSSTAGNGF VVTESDIVAN TRKGKQVMNV TMPDEAKLVV
     PVKGDHLAVV GENRKMLVFP LVQVPEMARG KGVRLQRYKD GGVSDIRSFA IAEGLTWEDS
     AGRVFTKTRD ELIEWMGDRA GAGRVVPKGF PRSGKFSG