PARC_RICPR
ID PARC_RICPR Reviewed; 738 AA.
AC Q9ZE79;
DT 30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-1999, sequence version 1.
DT 25-MAY-2022, entry version 122.
DE RecName: Full=DNA topoisomerase 4 subunit A {ECO:0000255|HAMAP-Rule:MF_00936};
DE EC=5.6.2.2 {ECO:0000255|HAMAP-Rule:MF_00936};
DE AltName: Full=Topoisomerase IV subunit A {ECO:0000255|HAMAP-Rule:MF_00936};
GN Name=parC {ECO:0000255|HAMAP-Rule:MF_00936}; OrderedLocusNames=RP067;
OS Rickettsia prowazekii (strain Madrid E).
OC Bacteria; Proteobacteria; Alphaproteobacteria; Rickettsiales;
OC Rickettsiaceae; Rickettsieae; Rickettsia; typhus group.
OX NCBI_TaxID=272947;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Madrid E;
RX PubMed=9823893; DOI=10.1038/24094;
RA Andersson S.G.E., Zomorodipour A., Andersson J.O., Sicheritz-Ponten T.,
RA Alsmark U.C.M., Podowski R.M., Naeslund A.K., Eriksson A.-S., Winkler H.H.,
RA Kurland C.G.;
RT "The genome sequence of Rickettsia prowazekii and the origin of
RT mitochondria.";
RL Nature 396:133-140(1998).
CC -!- FUNCTION: Topoisomerase IV is essential for chromosome segregation. It
CC relaxes supercoiled DNA. Performs the decatenation events required
CC during the replication of a circular DNA molecule. {ECO:0000255|HAMAP-
CC Rule:MF_00936}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP-dependent breakage, passage and rejoining of double-
CC stranded DNA.; EC=5.6.2.2; Evidence={ECO:0000255|HAMAP-
CC Rule:MF_00936};
CC -!- SUBUNIT: Heterotetramer composed of ParC and ParE. {ECO:0000255|HAMAP-
CC Rule:MF_00936}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000255|HAMAP-Rule:MF_00936};
CC Peripheral membrane protein {ECO:0000255|HAMAP-Rule:MF_00936}.
CC -!- SIMILARITY: Belongs to the type II topoisomerase GyrA/ParC subunit
CC family. ParC type 1 subfamily. {ECO:0000255|HAMAP-Rule:MF_00936}.
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DR EMBL; AJ235270; CAA14538.1; -; Genomic_DNA.
DR PIR; C71715; C71715.
DR RefSeq; NP_220461.1; NC_000963.1.
DR RefSeq; WP_004599725.1; NC_000963.1.
DR AlphaFoldDB; Q9ZE79; -.
DR SMR; Q9ZE79; -.
DR STRING; 272947.RP067; -.
DR EnsemblBacteria; CAA14538; CAA14538; CAA14538.
DR GeneID; 57569195; -.
DR KEGG; rpr:RP067; -.
DR PATRIC; fig|272947.5.peg.68; -.
DR eggNOG; COG0188; Bacteria.
DR HOGENOM; CLU_002977_4_1_5; -.
DR OMA; PRSNRID; -.
DR Proteomes; UP000002480; Chromosome.
DR GO; GO:0005694; C:chromosome; IEA:InterPro.
DR GO; GO:0019897; C:extrinsic component of plasma membrane; IEA:UniProtKB-UniRule.
DR GO; GO:0005524; F:ATP binding; IEA:InterPro.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003918; F:DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) activity; IEA:UniProtKB-UniRule.
DR GO; GO:0007059; P:chromosome segregation; IEA:UniProtKB-UniRule.
DR GO; GO:0006265; P:DNA topological change; IEA:UniProtKB-UniRule.
DR CDD; cd00187; TOP4c; 1.
DR Gene3D; 1.10.268.10; -; 1.
DR Gene3D; 2.120.10.90; -; 1.
DR Gene3D; 3.90.199.10; -; 1.
DR HAMAP; MF_00936; ParC_type1; 1.
DR InterPro; IPR006691; GyrA/parC_rep.
DR InterPro; IPR035516; Gyrase/topoIV_suA_C.
DR InterPro; IPR013760; Topo_IIA-like_dom_sf.
DR InterPro; IPR013758; Topo_IIA_A/C_ab.
DR InterPro; IPR013757; Topo_IIA_A_a_sf.
DR InterPro; IPR002205; Topo_IIA_dom_A.
DR InterPro; IPR005742; TopoIV_A_Gneg.
DR Pfam; PF03989; DNA_gyraseA_C; 3.
DR Pfam; PF00521; DNA_topoisoIV; 1.
DR SMART; SM00434; TOP4c; 1.
DR SUPFAM; SSF101904; SSF101904; 1.
DR SUPFAM; SSF56719; SSF56719; 1.
PE 3: Inferred from homology;
KW Cell membrane; DNA-binding; Isomerase; Membrane; Reference proteome;
KW Topoisomerase.
FT CHAIN 1..738
FT /note="DNA topoisomerase 4 subunit A"
FT /id="PRO_0000145406"
FT ACT_SITE 120
FT /note="O-(5'-phospho-DNA)-tyrosine intermediate"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00936"
FT SITE 40
FT /note="Interaction with DNA"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00936"
FT SITE 76
FT /note="Interaction with DNA"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00936"
FT SITE 78
FT /note="Interaction with DNA"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00936"
FT SITE 119
FT /note="Transition state stabilizer"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00936"
SQ SEQUENCE 738 AA; 83348 MW; 166B9B88C76E52A8 CRC64;
MKEAKIENID FGNALSERYL AYALSTIMSR SLPDVRDGLK PVHRRLLYAM LQLRLEPNSG
YKKCARVVGD VIGKYHPHGD VAVYDTLVRL AQHFSLRYPL IDGQGNFGSI DGDNAAAMRY
TESRMTEICM LLMEDIDKDT VDFRSTYDDS DLEPVIMPAS FPNLLANGSE GIAVGMATNI
PPHNLHELCD ALLYLIDNPQ AGINDIMNFI KGPDFPTGGI IIDKAEVINA AYTTGRGSFR
VRSRWEKEEL SYGTYQIVVT EIPYQIQKSK LIEQIAILLK DKKIPLISSI RDESTDIIRV
VIEPRDRSCD PQIVMESLFK LTNLESRIQL NMNVIGSNNV PRVMNILEIL QEFLVHRKNI
IIRRSTYLLN KIKQRLEILK VLRIVYLNLD EIIEIIREED EPKTIIMERF KISAIQVEVI
LNTRLRSLQK LEEHAIIDEH SNLQKQQAIL EKILKNHKEL WQIVKKEIKA VQTKFGLNTI
IGARRTSFEE VDLTNQVVDI TAFITKEPIT IICSKMGWVR SLKGHNTDLS TIKYKEGDTE
KFIIEAYTTD KILIISSKGR FFTLLADNIS KGKGTGGVSI KLLVDIGNND ITNILVYKPN
QLLLLASSIG KGFLVNSNEV IAQTKTGKQI MNIPEGYSCI ACLPVNGDSI ACIGESRRLL
VFNIDEIPEM KKGQGVVLQR FKNAKLLDIK IFNKQDGLSW NDGTKIQLEK NIVAFLGKRG
GFGTFPPIGF PKNNRFSP