ID   PARC_STRP1              Reviewed;         819 AA.
AC   Q9L7Q4; Q48Z88;
DT   23-JAN-2002, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2000, sequence version 1.
DT   25-MAY-2022, entry version 147.
DE   RecName: Full=DNA topoisomerase 4 subunit A {ECO:0000255|HAMAP-Rule:MF_00937};
DE            EC=5.6.2.2 {ECO:0000255|HAMAP-Rule:MF_00937};
DE   AltName: Full=Topoisomerase IV subunit A {ECO:0000255|HAMAP-Rule:MF_00937};
GN   Name=parC {ECO:0000255|HAMAP-Rule:MF_00937};
GN   OrderedLocusNames=SPy_0910, M5005_Spy0712;
OS   Streptococcus pyogenes serotype M1.
OC   Bacteria; Firmicutes; Bacilli; Lactobacillales; Streptococcaceae;
OC   Streptococcus.
OX   NCBI_TaxID=301447;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 700294 / SF370 / Serotype M1;
RA   Yan S.S., Gill V.J., Fedorko D.P.;
RT   "Resistance to multiple fluoroquinolone antibiotics in a clinical isolate
RT   of Streptococcus pyogenes: identification of gyrA and parC genes and point
RT   mutations responsible for the resistances.";
RL   Submitted (JAN-2000) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 700294 / SF370 / Serotype M1;
RX   PubMed=11296296; DOI=10.1073/pnas.071559398;
RA   Ferretti J.J., McShan W.M., Ajdic D.J., Savic D.J., Savic G., Lyon K.,
RA   Primeaux C., Sezate S., Suvorov A.N., Kenton S., Lai H.S., Lin S.P.,
RA   Qian Y., Jia H.G., Najar F.Z., Ren Q., Zhu H., Song L., White J., Yuan X.,
RA   Clifton S.W., Roe B.A., McLaughlin R.E.;
RT   "Complete genome sequence of an M1 strain of Streptococcus pyogenes.";
RL   Proc. Natl. Acad. Sci. U.S.A. 98:4658-4663(2001).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC BAA-947 / MGAS5005 / Serotype M1;
RX   PubMed=16088826; DOI=10.1086/432514;
RA   Sumby P., Porcella S.F., Madrigal A.G., Barbian K.D., Virtaneva K.,
RA   Ricklefs S.M., Sturdevant D.E., Graham M.R., Vuopio-Varkila J., Hoe N.P.,
RA   Musser J.M.;
RT   "Evolutionary origin and emergence of a highly successful clone of serotype
RT   M1 group A Streptococcus involved multiple horizontal gene transfer
RT   events.";
RL   J. Infect. Dis. 192:771-782(2005).
CC   -!- FUNCTION: Topoisomerase IV is essential for chromosome segregation. It
CC       relaxes supercoiled DNA. Performs the decatenation events required
CC       during the replication of a circular DNA molecule. {ECO:0000255|HAMAP-
CC       Rule:MF_00937}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP-dependent breakage, passage and rejoining of double-
CC         stranded DNA.; EC=5.6.2.2; Evidence={ECO:0000255|HAMAP-
CC         Rule:MF_00937};
CC   -!- SUBUNIT: Heterotetramer composed of ParC and ParE. {ECO:0000255|HAMAP-
CC       Rule:MF_00937}.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000255|HAMAP-Rule:MF_00937};
CC       Peripheral membrane protein {ECO:0000255|HAMAP-Rule:MF_00937}.
CC   -!- SIMILARITY: Belongs to the type II topoisomerase GyrA/ParC subunit
CC       family. ParC type 2 subfamily. {ECO:0000255|HAMAP-Rule:MF_00937}.
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DR   EMBL; AF220946; AAF63267.1; -; Genomic_DNA.
DR   EMBL; AE004092; AAK33826.1; -; Genomic_DNA.
DR   EMBL; CP000017; AAZ51330.1; -; Genomic_DNA.
DR   RefSeq; NP_269105.1; NC_002737.2.
DR   AlphaFoldDB; Q9L7Q4; -.
DR   SMR; Q9L7Q4; -.
DR   STRING; 1314.HKU360_00722; -.
DR   PaxDb; Q9L7Q4; -.
DR   EnsemblBacteria; AAK33826; AAK33826; SPy_0910.
DR   KEGG; spy:SPy_0910; -.
DR   KEGG; spz:M5005_Spy0712; -.
DR   PATRIC; fig|160490.10.peg.782; -.
DR   HOGENOM; CLU_002977_6_1_9; -.
DR   OMA; PRSNRID; -.
DR   Proteomes; UP000000750; Chromosome.
DR   GO; GO:0005694; C:chromosome; IEA:InterPro.
DR   GO; GO:0019897; C:extrinsic component of plasma membrane; IEA:UniProtKB-UniRule.
DR   GO; GO:0005524; F:ATP binding; IEA:InterPro.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0003918; F:DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0007059; P:chromosome segregation; IEA:UniProtKB-UniRule.
DR   GO; GO:0006265; P:DNA topological change; IEA:UniProtKB-UniRule.
DR   CDD; cd00187; TOP4c; 1.
DR   Gene3D; 1.10.268.10; -; 1.
DR   Gene3D; 2.120.10.90; -; 1.
DR   Gene3D; 3.90.199.10; -; 1.
DR   HAMAP; MF_00937; ParC_type2; 1.
DR   InterPro; IPR006691; GyrA/parC_rep.
DR   InterPro; IPR035516; Gyrase/topoIV_suA_C.
DR   InterPro; IPR013760; Topo_IIA-like_dom_sf.
DR   InterPro; IPR013758; Topo_IIA_A/C_ab.
DR   InterPro; IPR013757; Topo_IIA_A_a_sf.
DR   InterPro; IPR002205; Topo_IIA_dom_A.
DR   InterPro; IPR005741; TopoIV_A_Gpos.
DR   PANTHER; PTHR43493:SF9; PTHR43493:SF9; 1.
DR   Pfam; PF03989; DNA_gyraseA_C; 4.
DR   Pfam; PF00521; DNA_topoisoIV; 1.
DR   SMART; SM00434; TOP4c; 1.
DR   SUPFAM; SSF101904; SSF101904; 1.
DR   SUPFAM; SSF56719; SSF56719; 1.
DR   TIGRFAMs; TIGR01061; parC_Gpos; 1.
PE   3: Inferred from homology;
KW   Cell membrane; DNA-binding; Isomerase; Membrane; Reference proteome;
KW   Topoisomerase.
FT   CHAIN           1..819
FT                   /note="DNA topoisomerase 4 subunit A"
FT                   /id="PRO_0000145419"
FT   ACT_SITE        118
FT                   /note="O-(5'-phospho-DNA)-tyrosine intermediate"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00937"
FT   SITE            38
FT                   /note="Interaction with DNA"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00937"
FT   SITE            74
FT                   /note="Interaction with DNA"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00937"
FT   SITE            76
FT                   /note="Interaction with DNA"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00937"
FT   SITE            87
FT                   /note="Interaction with DNA"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00937"
FT   SITE            93
FT                   /note="Interaction with DNA"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00937"
FT   SITE            117
FT                   /note="Transition state stabilizer"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00937"
FT   CONFLICT        798
FT                   /note="I -> T (in Ref. 3; AAZ51330)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   819 AA;  92478 MW;  E1402D11768347BC CRC64;
     MSNIQNMSLE DIMGERFGRY SKYIIQERAL PDIRDGLKPV QRRILYSMNK DGNTFEKGYR
     KSAKSVGNIM GNFHPHGDSS IYDAMVRMSQ DWKNREILVE MHGNNGSMDG DPPAAMRYTE
     ARLSEIAGYL LQDIEKNTVS FAWNFDDTEK EPTVLPAAFP NLLVNGSSGI SAGYATDIPP
     HNLSEVIDAV VYMIDHPKAS LEKLMEFLPG PDFPTGGIIQ GADEIKKAYE TGKGRVVVRS
     RTEIEELKGG KQQIIVTEIP YEVNKAVLVK KIDDVRVNNK VPGIVEVRDE SDRTGLRIAI
     ELKKEADSQT ILNYLLKYTD LQVNYNFNMV AIDHFTPRQV GLQKILSSYI SHRKDIIIER
     SKFDKAKAEK RLHIVEGLIR VLSILDEIIA LIRSSDNKAD AKENLKVSYD FSEEQAEAIV
     TLQLYRLTNT DIVTLQNEEN DLRDLITTLS AIIGDEATMY NVMKRELREV KKKFANPRLS
     ELQAESQIIE IDTASLIAEE ETFVSVTRGG YLKRTSPRSF NASSLEEVGK RDDDELIFVK
     QAKTTEHLLL FTTLGNVIYR PIHELTDLRW KDIGEHLSQT ISNFATEEEI LYADIVTSFD
     QGLYVAVTQN GFIKRFDRKE LSPWRTYKSK STKYVKLKDD KDRVVTLSPV IMEDLLLVTK
     NGYALRFSSQ EVPIQGLKSA GVKGINLKND DSLASAFAVT SNSFFVLTQR GSLKRMAVDD
     IPQTSRANRG LLVLRELKTK PHRVFLAGGV QSDTSAEQFD LFTDIPEEET NQQMLEVISK
     TGQTYEIALE TLSLSERISN GSFISDTISD QEVLVARTR