PARI_RAT
ID PARI_RAT Reviewed; 573 AA.
AC Q9EQ10;
DT 20-MAR-2007, integrated into UniProtKB/Swiss-Prot.
DT 20-MAR-2007, sequence version 2.
DT 03-AUG-2022, entry version 93.
DE RecName: Full=PCNA-interacting partner;
DE Short=PARI;
DE AltName: Full=Antisense RNA overlapping MCH gene protein;
DE Short=AROM;
DE AltName: Full=PARP-1 binding protein;
DE AltName: Full=PARP1-binding protein;
DE Short=PARPBP;
GN Name=Parpbp; Synonyms=Arom, Pari;
OS Rattus norvegicus (Rat).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Rattus.
OX NCBI_TaxID=10116;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), FUNCTION, SUBCELLULAR
RP LOCATION, AND TISSUE SPECIFICITY.
RC STRAIN=Wistar;
RX PubMed=11006283; DOI=10.1074/jbc.m006524200;
RA Borsu L., Presse F., Nahon J.-L.;
RT "The AROM gene, spliced mRNAs encoding new DNA/RNA-binding proteins are
RT transcribed from the opposite strand of the melanin-concentrating hormone
RT gene in mammals.";
RL J. Biol. Chem. 275:40576-40587(2000).
CC -!- FUNCTION: Required to suppress inappropriate homologous recombination,
CC thereby playing a central role DNA repair and in the maintenance of
CC genomic stability. Antagonizes homologous recombination by interfering
CC with the formation of the RAD51-DNA homologous recombination structure.
CC Positively regulate the poly(ADP-ribosyl)ation activity of PARP1;
CC however such function may be indirect (By similarity). Binds single-
CC strand DNA and poly(A) homopolymers. {ECO:0000250,
CC ECO:0000269|PubMed:11006283}.
CC -!- SUBUNIT: Interacts with RAD51 and PCNA. Interacts with PARP1 (By
CC similarity). Interacts with TASOR (By similarity).
CC {ECO:0000250|UniProtKB:Q6IRT3, ECO:0000250|UniProtKB:Q9NWS1}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:11006283}. Nucleus
CC {ECO:0000269|PubMed:11006283}. Note=Localizes to chromatin in response
CC to S phase arrest but not in mitosis. Targeted to chromatin via its
CC interaction with PCNA (By similarity). {ECO:0000250}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1; Synonyms=AROM-p64;
CC IsoId=Q9EQ10-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q9EQ10-2; Sequence=VSP_023598;
CC -!- TISSUE SPECIFICITY: Present in testis (at protein level). Expressed in
CC testis, gastrointestinal tract (jejunum, ileum, and colon) and immune
CC system (thymus and spleen). Weakly expressed in lung, kidney, pituitary
CC gland and muscle. {ECO:0000269|PubMed:11006283}.
CC -!- MISCELLANEOUS: The gene encoding Arom is located in the opposite strand
CC of the gene encoding MCH.
CC -!- SIMILARITY: Belongs to the PARI family. {ECO:0000305}.
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DR EMBL; AF303035; AAG42418.1; -; mRNA.
DR RefSeq; NP_001160148.1; NM_001166676.1. [Q9EQ10-2]
DR RefSeq; XP_006241250.1; XM_006241188.3. [Q9EQ10-1]
DR RefSeq; XP_006241251.1; XM_006241189.3. [Q9EQ10-1]
DR RefSeq; XP_006241252.1; XM_006241190.3. [Q9EQ10-1]
DR AlphaFoldDB; Q9EQ10; -.
DR SMR; Q9EQ10; -.
DR STRING; 10116.ENSRNOP00000006249; -.
DR PaxDb; Q9EQ10; -.
DR PRIDE; Q9EQ10; -.
DR Ensembl; ENSRNOT00000065899; ENSRNOP00000060859; ENSRNOG00000004682. [Q9EQ10-2]
DR GeneID; 100310874; -.
DR KEGG; rno:100310874; -.
DR UCSC; RGD:2314264; rat. [Q9EQ10-1]
DR CTD; 55010; -.
DR RGD; 2314264; Parpbp.
DR VEuPathDB; HostDB:ENSRNOG00000004682; -.
DR eggNOG; ENOG502QR2U; Eukaryota.
DR GeneTree; ENSGT00390000006088; -.
DR HOGENOM; CLU_034470_1_0_1; -.
DR InParanoid; Q9EQ10; -.
DR PhylomeDB; Q9EQ10; -.
DR TreeFam; TF332230; -.
DR PRO; PR:Q9EQ10; -.
DR Proteomes; UP000002494; Chromosome 7.
DR Bgee; ENSRNOG00000004682; Expressed in testis and 13 other tissues.
DR ExpressionAtlas; Q9EQ10; baseline and differential.
DR Genevisible; Q9EQ10; RN.
DR GO; GO:0000785; C:chromatin; ISS:UniProtKB.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0006281; P:DNA repair; IEA:UniProtKB-KW.
DR GO; GO:2000042; P:negative regulation of double-strand break repair via homologous recombination; ISS:UniProtKB.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR038932; PARPBP.
DR PANTHER; PTHR32121; PTHR32121; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Cytoplasm; DNA damage; DNA repair; DNA-binding;
KW Nucleus; Reference proteome.
FT CHAIN 1..573
FT /note="PCNA-interacting partner"
FT /id="PRO_0000280271"
FT REGION 463..511
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 479..497
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 1..81
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:11006283"
FT /id="VSP_023598"
SQ SEQUENCE 573 AA; 63902 MW; A7D437F502918992 CRC64;
MAVLNQMSVL DMIKAFRRNW RAHCNSERTT LCGPDSMLLA LQLSMAENNK QHHGEFTVCL
SDVLLTWKYF LHEKLNLPIE NMKVVDHYED IRKTYDDFLK NSNTLDLIDV YKKCSIMTSN
CEKHMISSIQ LRDFLSGTEC AVNDENNPHS PASPVKCSQN DKQVRLLAKK IFLSYLSLLV
NSKNDLALAQ ILNTPDRGLG REAFTNLKHA AREKQLSMFL MATSFIRTLE LGGKGYAPPP
SDPLWAHVKG LSAFVNFIDK LNEILGEVPN PSLAGGRILS AIRTQLIKGH SSGEPFYKAV
EEVVQDLNLR IKNIINSQEG VTVSATNISP VPPKSFAINH DTAYCGRDAV KILLVLLDED
AASAPTRNKA ELLYNDQSTA PHGGASVLTL FRSPTQVKST PVKPLRERIH TSLQAEKNKQ
MRQTLIRSQF ACTYKDDCIM SKSKWNVNSS SKPLSALRLE NDVSEGAQPS VGKARLETSS
ENVHVDRSKD DKGPRKSTKR KLAKSKQPGV REDIHCDQGD ELYQPKNVKI LKVPSDSHRK
AGGKLAPGAR GNRCTTKDKL IPGQTKLTRF FML
