ID   PARS_SPHYB              Reviewed;         159 AA.
AC   A0A0C5XKJ0;
DT   13-NOV-2019, integrated into UniProtKB/Swiss-Prot.
DT   29-APR-2015, sequence version 1.
DT   25-MAY-2022, entry version 22.
DE   RecName: Full=Prs ADP-ribosylating antitoxin {ECO:0000303|PubMed:30598453};
GN   Name=parS {ECO:0000303|PubMed:30598453};
GN   Synonyms=yblJ {ECO:0000303|PubMed:30598453}; ORFNames=TZ53_17665;
OS   Sphingobium sp. (strain YBL2).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Sphingomonadales;
OC   Sphingomonadaceae; Sphingobium.
OX   NCBI_TaxID=484429;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=YBL2;
RA   Yan X.;
RT   "The analysis of one genome.";
RL   Submitted (FEB-2015) to the EMBL/GenBank/DDBJ databases.
RN   [2] {ECO:0007744|PDB:6D0H, ECO:0007744|PDB:6D0I}
RP   X-RAY CRYSTALLOGRAPHY (1.50 ANGSTROMS) OF 88-159 IN COMPLEX WITH TOXIN,
RP   FUNCTION AS AN ANTITOXIN, AND DOMAIN.
RC   STRAIN=YBL2;
RX   PubMed=30598453; DOI=10.1073/pnas.1814633116;
RA   Piscotta F.J., Jeffrey P.D., Link A.J.;
RT   "ParST is a widespread toxin-antitoxin module that targets nucleotide
RT   metabolism.";
RL   Proc. Natl. Acad. Sci. U.S.A. 116:826-834(2019).
CC   -!- FUNCTION: Antitoxin component of a type II toxin-antitoxin (TA) system.
CC       Neutralizes the bacteriostatic effect of cognate toxin ParT by
CC       inserting into its active site. {ECO:0000269|PubMed:30598453}.
CC   -!- SUBUNIT: Forms heterotetrameric ParS(2)-ParT(2) complexes. The 2
CC       antitoxin fragments do not make contact in the crystal structure.
CC       {ECO:0000269|PubMed:30598453}.
CC   -!- DOMAIN: The C-terminus (residues 99-159) is sufficient to bind and
CC       neutralize toxin when expressed in E.coli.
CC       {ECO:0000269|PubMed:30598453}.
CC   -!- SIMILARITY: Belongs to the MbcA/ParS/Xre antitoxin family.
CC       {ECO:0000305}.
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DR   EMBL; CP010954; AJR25281.1; -; Genomic_DNA.
DR   RefSeq; WP_044662137.1; NZ_CP010954.1.
DR   PDB; 6D0H; X-ray; 1.50 A; B/D=88-159.
DR   PDB; 6D0I; X-ray; 1.55 A; B/D=88-159.
DR   PDBsum; 6D0H; -.
DR   PDBsum; 6D0I; -.
DR   AlphaFoldDB; A0A0C5XKJ0; -.
DR   SMR; A0A0C5XKJ0; -.
DR   STRING; 484429.TZ53_17665; -.
DR   EnsemblBacteria; AJR25281; AJR25281; TZ53_17665.
DR   KEGG; syb:TZ53_17665; -.
DR   PATRIC; fig|484429.4.peg.3700; -.
DR   HOGENOM; CLU_109353_0_0_5; -.
DR   OrthoDB; 1850771at2; -.
DR   Proteomes; UP000032302; Chromosome.
DR   InterPro; IPR024467; Xre/MbcA/ParS-like_toxin-bd.
DR   Pfam; PF09722; DUF2384; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Toxin-antitoxin system.
FT   CHAIN           1..159
FT                   /note="Prs ADP-ribosylating antitoxin"
FT                   /id="PRO_0000448600"
FT   REGION          99..159
FT                   /note="Sufficient to neutralize toxin"
FT                   /evidence="ECO:0000269|PubMed:30598453"
FT   HELIX           91..105
FT                   /evidence="ECO:0007829|PDB:6D0H"
FT   HELIX           113..121
FT                   /evidence="ECO:0007829|PDB:6D0H"
FT   HELIX           126..128
FT                   /evidence="ECO:0007829|PDB:6D0H"
FT   HELIX           133..136
FT                   /evidence="ECO:0007829|PDB:6D0H"
FT   HELIX           140..155
FT                   /evidence="ECO:0007829|PDB:6D0H"
SQ   SEQUENCE   159 AA;  17037 MW;  7126E4A940B69448 CRC64;
     MSELETGARK AQGKGTPPFA YKALYRASPV DRIGVIKKGV AASDLKRFIA ALHVDQKVMF
     DALNLKTATV NKKAANNQPL STEDSERVLG LAKLVGQLED MVEESGETDG FDAPEWLSSW
     LRQPLPALGG VNPIDLLDTM EGQAVVSRAL AQIQSGAFA