ID   A36_VAR67               Reviewed;         216 AA.
AC   P33852;
DT   01-FEB-1994, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1994, sequence version 1.
DT   03-AUG-2022, entry version 63.
DE   RecName: Full=Protein A36;
GN   ORFNames=A36R, A39R;
OS   Variola virus (isolate Human/India/Ind3/1967) (VARV) (Smallpox virus).
OC   Viruses; Varidnaviria; Bamfordvirae; Nucleocytoviricota; Pokkesviricetes;
OC   Chitovirales; Poxviridae; Chordopoxvirinae; Orthopoxvirus.
OX   NCBI_TaxID=587200;
OH   NCBI_TaxID=9606; Homo sapiens (Human).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=India-1967 / Isolate Ind3;
RX   PubMed=1666548;
RA   Shchelkunov S.N., Marennikova S.S., Totmenin A.V., Blinov V.M.,
RA   Chizhikov V.E., Gutorov V.V., Safronov P.F., Pozdnyakov S.G.,
RA   Shelukhina E.M., Gashnikov P.V., Anjaparidze O.G., Sandakhchiev L.S.;
RT   "Creation of a clone library of fragments from the natural variola virus
RT   and study of the structural and functional organization of viral genes from
RT   a circle of hosts.";
RL   Dokl. Akad. Nauk SSSR 321:402-406(1991).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=India-1967 / Isolate Ind3;
RX   PubMed=8384129; DOI=10.1016/0014-5793(93)80041-r;
RA   Shchelkunov S.N., Blinov V.M., Sandakhchiev L.S.;
RT   "Genes of variola and vaccinia viruses necessary to overcome the host
RT   protective mechanisms.";
RL   FEBS Lett. 319:80-83(1993).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=Harvey;
RX   PubMed=1331292; DOI=10.1099/0022-1317-73-11-2887;
RA   Aguado B., Selmes I.P., Smith G.L.;
RT   "Nucleotide sequence of 21.8 kbp of variola major virus strain Harvey and
RT   comparison with vaccinia virus.";
RL   J. Gen. Virol. 73:2887-2902(1992).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=Garcia-1966;
RA   Shchelkunov S.N., Totmenin A.V., Resenchuk S.M., Blinov V.M.,
RA   Sandakhchiev L.S.;
RL   Submitted (DEC-1995) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Involved in the intracellular transport of virions to the
CC       host cell surface. Participates also in the formation of actin tails at
CC       the plasma membrane to allow efficient actin-based motility and thus
CC       cell to cell transmission of viral particles. Mechanistically,
CC       phosphorylation of Tyr-112 and 132 of A36 activates the host ARP2-ARP3
CC       complex and leads to actin nucleation (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Interacts with host NCK. Interacts with protein A33 (via C-
CC       terminus). Interacts with protein F12. Interacts (via C-terminus) with
CC       host kinesin light chain 1 (KLC1) (By similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Host membrane {ECO:0000305}; Single-pass membrane
CC       protein {ECO:0000305}. Note=Found exclusively on the wrapped enveloped
CC       virion. Absent in the mature virion (MV) and extracellular enveloped
CC       virion (EV) (By similarity). {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the orthopoxvirus A36 protein family.
CC       {ECO:0000305}.
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DR   EMBL; X69198; CAA49084.1; -; Genomic_DNA.
DR   EMBL; X67115; CAA47510.1; -; Genomic_DNA.
DR   EMBL; X76266; CAA53865.1; -; Genomic_DNA.
DR   PIR; D36852; D36852.
DR   PIR; E72168; E72168.
DR   RefSeq; NP_042187.1; NC_001611.1.
DR   GeneID; 1486517; -.
DR   KEGG; vg:1486517; -.
DR   Proteomes; UP000002060; Genome.
DR   GO; GO:0033644; C:host cell membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   InterPro; IPR010274; Orthopox_A36R.
DR   Pfam; PF05950; Orthopox_A36R; 1.
PE   3: Inferred from homology;
KW   Host membrane; Host-virus interaction; Membrane; Phosphoprotein;
KW   Reference proteome; Transmembrane; Transmembrane helix.
FT   CHAIN           1..216
FT                   /note="Protein A36"
FT                   /id="PRO_0000040601"
FT   TOPO_DOM        1
FT                   /note="Extracellular"
FT   TRANSMEM        2..22
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        23..216
FT                   /note="Cytoplasmic"
FT   MOD_RES         112
FT                   /note="Phosphotyrosine; by host"
FT                   /evidence="ECO:0000250"
FT   MOD_RES         132
FT                   /note="Phosphotyrosine; by host"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   216 AA;  24492 MW;  76B5BF04AB3AC471 CRC64;
     MILVPLITVT VVAGTILVCY ILYICRKKIR TVYNDNKIIM TKLKKIKSSN SSKSSKSTDN
     ESDWEDHCSA MEQNNDVDNI SRNEILDDDS FAGSLIWDNE SNVIAPSTEH IYDSVAGSTR
     LINNDCNEQT IYQNTTVINE TETIEVLNED TKQNPSYSSN PFVNYNKTSI CSKSNPFITE
     LNNKFSENNP FRRAHSDDYL NKQEHDDIES SVVSLV