ID   PASI_ECOL6              Reviewed;          96 AA.
AC   Q8FEY5;
DT   30-MAY-2003, integrated into UniProtKB/Swiss-Prot.
DT   30-MAY-2003, sequence version 2.
DT   25-MAY-2022, entry version 83.
DE   RecName: Full=Persistence and stress-resistance antitoxin PasI;
DE   AltName: Full=Antitoxin PasI;
DE   AltName: Full=UPF0125 protein PasI;
GN   Name=pasI; Synonyms=ratB, yfjF; OrderedLocusNames=c3140;
OS   Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=199310;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=CFT073 / ATCC 700928 / UPEC;
RX   PubMed=12471157; DOI=10.1073/pnas.252529799;
RA   Welch R.A., Burland V., Plunkett G. III, Redford P., Roesch P., Rasko D.,
RA   Buckles E.L., Liou S.-R., Boutin A., Hackett J., Stroud D., Mayhew G.F.,
RA   Rose D.J., Zhou S., Schwartz D.C., Perna N.T., Mobley H.L.T.,
RA   Donnenberg M.S., Blattner F.R.;
RT   "Extensive mosaic structure revealed by the complete genome sequence of
RT   uropathogenic Escherichia coli.";
RL   Proc. Natl. Acad. Sci. U.S.A. 99:17020-17024(2002).
RN   [2]
RP   FUNCTION AS AN ANTITOXIN, AND DISRUPTION PHENOTYPE.
RC   STRAIN=CFT073 / ATCC 700928 / UPEC;
RX   PubMed=23055930; DOI=10.1371/journal.ppat.1002954;
RA   Norton J.P., Mulvey M.A.;
RT   "Toxin-antitoxin systems are important for niche-specific colonization and
RT   stress resistance of uropathogenic Escherichia coli.";
RL   PLoS Pathog. 8:E1002954-E1002954(2012).
CC   -!- FUNCTION: Antitoxin component of a type II toxin-antitoxin (TA) system.
CC       Unlike the case in E.coli K12 high level expression of this protein
CC       abrogates the toxic effects due to overexpression of the cognate toxin
CC       PasT. {ECO:0000269|PubMed:23055930}.
CC   -!- DISRUPTION PHENOTYPE: Deletion of the pasT-pasI operon causes reduced
CC       infection of the mouse kidney but not of the bladder. On rich medium
CC       operon deletion decreases bacterial persistence about 100-fold. Forms
CC       small colonies on rich medium. Delays growth on minimal medium
CC       supplemented with threonine, increased sensitivity to oxidative and
CC       nitrosative stress. Growth defects are complemented by low levels of
CC       PasT expression. {ECO:0000269|PubMed:23055930}.
CC   -!- SIMILARITY: Belongs to the UPF0125 (RnfH) family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAN81590.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; AE014075; AAN81590.1; ALT_INIT; Genomic_DNA.
DR   RefSeq; WP_001117834.1; NC_004431.1.
DR   AlphaFoldDB; Q8FEY5; -.
DR   SMR; Q8FEY5; -.
DR   STRING; 199310.c3140; -.
DR   EnsemblBacteria; AAN81590; AAN81590; c3140.
DR   KEGG; ecc:c3140; -.
DR   eggNOG; COG2914; Bacteria.
DR   HOGENOM; CLU_150721_1_1_6; -.
DR   OMA; DMIHVEV; -.
DR   Proteomes; UP000001410; Chromosome.
DR   Gene3D; 3.10.20.280; -; 1.
DR   HAMAP; MF_00460; UPF0125_RnfH; 1.
DR   InterPro; IPR016155; Mopterin_synth/thiamin_S_b.
DR   InterPro; IPR005346; RnfH.
DR   InterPro; IPR037021; RnfH_sf.
DR   PANTHER; PTHR37483; PTHR37483; 1.
DR   Pfam; PF03658; Ub-RnfH; 1.
DR   SUPFAM; SSF54285; SSF54285; 1.
PE   1: Evidence at protein level;
KW   Toxin-antitoxin system.
FT   CHAIN           1..96
FT                   /note="Persistence and stress-resistance antitoxin PasI"
FT                   /id="PRO_0000192488"
SQ   SEQUENCE   96 AA;  10815 MW;  68AB79B00E238F03 CRC64;
     MPGKIAVEVA YALPEKQYLQ RVTLQEGATV EEAIRASGLL ELRTDIDLTK NKVGIYSRPA
     KLSDIVHDGD RVEIYRPLIA DPKELRRQRA EKSANK