ID   PASM1_LACPN             Reviewed;          64 AA.
AC   C7G1H4;
DT   15-JUN-2010, integrated into UniProtKB/Swiss-Prot.
DT   13-OCT-2009, sequence version 1.
DT   25-MAY-2022, entry version 19.
DE   RecName: Full=Bacteriocin plantaricin ASM1 {ECO:0000303|PubMed:19939484};
DE   Flags: Precursor;
GN   Name=bactA1 {ECO:0000312|EMBL:BAH98036.1};
OS   Lactiplantibacillus plantarum (Lactobacillus plantarum).
OC   Bacteria; Firmicutes; Bacilli; Lactobacillales; Lactobacillaceae;
OC   Lactiplantibacillus.
OX   NCBI_TaxID=1590;
RN   [1] {ECO:0000305, ECO:0000312|EMBL:BAH98036.1}
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 22-63, FUNCTION,
RP   BIOPHYSICOCHEMICAL PROPERTIES, SUBCELLULAR LOCATION, DISULFIDE BONDS, AND
RP   MASS SPECTROMETRY.
RC   STRAIN=A-1 {ECO:0000303|PubMed:19939484};
RX   PubMed=19939484; DOI=10.1016/j.ijfoodmicro.2009.10.021;
RA   Hata T., Tanaka R., Ohmomo S.;
RT   "Isolation and characterization of plantaricin ASM1: a new bacteriocin
RT   produced by Lactobacillus plantarum A-1.";
RL   Int. J. Food Microbiol. 137:94-99(2010).
CC   -!- FUNCTION: Bacteriocin with a narrow antibacterial spectrum.
CC       Antibacterial activity against the Gram-positive bacteria L.plantarun,
CC       L.pentosus, L.curvatus, L.lindneri, L.mesenteroides and E.faecilis.
CC       Lacks antibacterial activity against the Gram-positive bacteria
CC       L.brevis, L.sakei, L.lactis, P.acidilactici, B.subtilis, B.cereus,
CC       L.monocytogenes and S.aureus, and against the Gram-negative bacteria
CC       E.coli and S.typhimurium. {ECO:0000269|PubMed:19939484}.
CC   -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC       pH dependence:
CC         Stable from pH 5.5-8.5. {ECO:0000269|PubMed:19939484};
CC       Temperature dependence:
CC         Thermostable, activity is retained after incubation at 90 degrees
CC         Celsius for 15 minutes. {ECO:0000269|PubMed:19939484};
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:19939484}.
CC   -!- PTM: Contains 2 disulfide bonds. {ECO:0000305}.
CC   -!- MASS SPECTROMETRY: Mass=5045.72; Method=MALDI;
CC       Evidence={ECO:0000269|PubMed:19939484};
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DR   EMBL; AB474371; BAH98036.1; -; Genomic_DNA.
DR   PDB; 2MVI; NMR; -; A=22-64.
DR   PDBsum; 2MVI; -.
DR   AlphaFoldDB; C7G1H4; -.
DR   BMRB; C7G1H4; -.
DR   SMR; C7G1H4; -.
DR   GO; GO:0005576; C:extracellular region; IDA:UniProtKB.
DR   GO; GO:0019835; P:cytolysis; IEA:UniProtKB-KW.
DR   GO; GO:0050830; P:defense response to Gram-positive bacterium; IDA:UniProtKB.
PE   1: Evidence at protein level;
KW   3D-structure; Antibiotic; Antimicrobial; Bacteriocin;
KW   Direct protein sequencing; Disulfide bond; Secreted; Signal;
KW   Thioether bond.
FT   SIGNAL          1..21
FT                   /evidence="ECO:0000269|PubMed:19939484"
FT   CHAIN           22..64
FT                   /note="Bacteriocin plantaricin ASM1"
FT                   /evidence="ECO:0000269|PubMed:19939484"
FT                   /id="PRO_0000394801"
FT   CROSSLNK        61..64
FT                   /note="Lanthionine (Ser-Cys)"
FT                   /evidence="ECO:0000269|PubMed:19939484"
FT   TURN            33..36
FT                   /evidence="ECO:0007829|PDB:2MVI"
FT   STRAND          37..40
FT                   /evidence="ECO:0007829|PDB:2MVI"
FT   TURN            45..49
FT                   /evidence="ECO:0007829|PDB:2MVI"
SQ   SEQUENCE   64 AA;  6891 MW;  04CBEF1151F8448A CRC64;
     MSKLVKTLTV DEISKIQTNG GKPAWCWYTL AMCGAGYDSG TCDYMYSHCF GVKHSSGGGG
     SYHC