ID   PAST_ECOL6              Reviewed;         158 AA.
AC   Q8FEY4;
DT   09-JAN-2013, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2003, sequence version 1.
DT   03-AUG-2022, entry version 79.
DE   RecName: Full=Persistence and stress-resistance toxin PasT;
DE   AltName: Full=Ribosome association toxin RatA;
GN   Name=pasT; Synonyms=ratA, yfjG; OrderedLocusNames=c3141;
OS   Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=199310;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=CFT073 / ATCC 700928 / UPEC;
RX   PubMed=12471157; DOI=10.1073/pnas.252529799;
RA   Welch R.A., Burland V., Plunkett G. III, Redford P., Roesch P., Rasko D.,
RA   Buckles E.L., Liou S.-R., Boutin A., Hackett J., Stroud D., Mayhew G.F.,
RA   Rose D.J., Zhou S., Schwartz D.C., Perna N.T., Mobley H.L.T.,
RA   Donnenberg M.S., Blattner F.R.;
RT   "Extensive mosaic structure revealed by the complete genome sequence of
RT   uropathogenic Escherichia coli.";
RL   Proc. Natl. Acad. Sci. U.S.A. 99:17020-17024(2002).
RN   [2]
RP   FUNCTION AS A TOXIN, ROLE IN STRESS RESPONSE, DISRUPTION PHENOTYPE, AND
RP   MUTAGENESIS OF 1-MET--LEU-9.
RC   STRAIN=CFT073 / ATCC 700928 / UPEC;
RX   PubMed=23055930; DOI=10.1371/journal.ppat.1002954;
RA   Norton J.P., Mulvey M.A.;
RT   "Toxin-antitoxin systems are important for niche-specific colonization and
RT   stress resistance of uropathogenic Escherichia coli.";
RL   PLoS Pathog. 8:E1002954-E1002954(2012).
CC   -!- FUNCTION: Toxic component of a type II toxin-antitoxin (TA) system.
CC       Binds to 50S ribosomal subunits, preventing them from associating with
CC       30S subunits to form 70S ribosomes (By similarity). In this strain of
CC       E.coli low levels of PasT complement operon disruption, however high
CC       levels are toxic; their effects are abrogated by high level expression
CC       of cognate antitoxin PasI. Plays a role in persistence after antibiotic
CC       exposure and survival of nitrosative stress; the toxic and persistence
CC       phenotypes are conferred by the same N-terminal region of the protein,
CC       while the stress resistance effects can be uncoupled from them in
CC       deletion mutants. {ECO:0000250, ECO:0000269|PubMed:23055930}.
CC   -!- SUBUNIT: Associates with 50S ribosomes. {ECO:0000250}.
CC   -!- DISRUPTION PHENOTYPE: Deletion of the pasT-pasI operon yields a
CC       defective infection of mouse kidney but not bladder. On rich medium
CC       operon deletion decreases bacterial persistence after antibiotic
CC       exposure about 100-fold. Forms small colonies on rich medium. Delays
CC       growth on minimal medium supplemented with threonine, increased
CC       sensitivity to oxidative and nitrosative stress. Growth defects are
CC       complemented by low levels of PasT expression.
CC       {ECO:0000269|PubMed:23055930}.
CC   -!- MISCELLANEOUS: This strain of E.coli has only 6 recognizable intact TA
CC       systems, whereas E.coli MG1655 strain K12 has at least 16. This may
CC       explain in part the differences between the 2 strains.
CC   -!- SIMILARITY: Belongs to the ribosome association toxin RatA family.
CC       {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; AE014075; AAN81591.1; -; Genomic_DNA.
DR   RefSeq; WP_000600193.1; NC_004431.1.
DR   AlphaFoldDB; Q8FEY4; -.
DR   SMR; Q8FEY4; -.
DR   STRING; 199310.c3141; -.
DR   EnsemblBacteria; AAN81591; AAN81591; c3141.
DR   KEGG; ecc:c3141; -.
DR   eggNOG; COG2867; Bacteria.
DR   HOGENOM; CLU_079653_3_1_6; -.
DR   OMA; YIDGPFK; -.
DR   BioCyc; ECOL199310:C3141-MON; -.
DR   Proteomes; UP000001410; Chromosome.
DR   GO; GO:0048039; F:ubiquinone binding; IEA:InterPro.
DR   GO; GO:0045333; P:cellular respiration; IEA:InterPro.
DR   GO; GO:0006744; P:ubiquinone biosynthetic process; IEA:InterPro.
DR   CDD; cd07813; COQ10p_like; 1.
DR   Gene3D; 3.30.530.20; -; 1.
DR   InterPro; IPR044996; COQ10-like.
DR   InterPro; IPR005031; COQ10_START.
DR   InterPro; IPR023393; START-like_dom_sf.
DR   PANTHER; PTHR12901; PTHR12901; 1.
DR   Pfam; PF03364; Polyketide_cyc; 1.
PE   1: Evidence at protein level;
KW   Stress response; Toxin-antitoxin system.
FT   CHAIN           1..158
FT                   /note="Persistence and stress-resistance toxin PasT"
FT                   /id="PRO_0000420800"
FT   REGION          70..158
FT                   /note="Required for resistance of nitrosative stress but
FT                   not persistence or toxic effects"
FT   MUTAGEN         1..9
FT                   /note="Missing: Loss of toxicity, no longer rescues
FT                   persistence in disrupted mutants, but colonies are wild-
FT                   type."
FT                   /evidence="ECO:0000269|PubMed:23055930"
SQ   SEQUENCE   158 AA;  17686 MW;  9D51C48BF74D73C5 CRC64;
     MILFVGFLLM EIVMPQISRT ALVPYSAEQM YQLVNDVQSY PQFLPGCTGS RILESTPGQM
     TAAVDVSKAG ISKTFTTRNQ LTSNQSILMS LVDGPFKKLI GGWKFTPLSQ DACRIEFHLD
     FEFTNKLIEL AFGRVFKELA ANMVQAFTVR AKEVYSAR