PAS_STRIT
ID PAS_STRIT Reviewed; 1310 AA.
AC Q9KW51;
DT 07-JUL-2009, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2001, sequence version 2.
DT 03-AUG-2022, entry version 85.
DE RecName: Full=Probable cell-surface antigen I/II;
DE AltName: Full=PAa {ECO:0000303|PubMed:11267788};
DE Flags: Precursor;
GN Name=pas;
OS Streptococcus intermedius.
OC Bacteria; Firmicutes; Bacilli; Lactobacillales; Streptococcaceae;
OC Streptococcus; Streptococcus anginosus group.
OX NCBI_TaxID=1338;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=GAI-1157;
RX PubMed=11267788; DOI=10.1111/j.1574-6968.2001.tb10573.x;
RA Tamura H., Kikuchi T., Shirato R., Kato H.;
RT "Cloning and DNA sequencing of the surface protein antigen I/II (PAa) of
RT Streptococcus cricetus.";
RL FEMS Microbiol. Lett. 196:251-256(2001).
CC -!- SUBCELLULAR LOCATION: Secreted, cell wall {ECO:0000255|PROSITE-
CC ProRule:PRU00477}; Peptidoglycan-anchor {ECO:0000255|PROSITE-
CC ProRule:PRU00477}.
CC -!- SIMILARITY: Belongs to the antigen I/II family. {ECO:0000305}.
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DR EMBL; AB045140; BAA96878.2; -; Genomic_DNA.
DR PIR; C60339; C60339.
DR RefSeq; WP_003075937.1; NZ_LS483436.1.
DR PDB; 6E36; X-ray; 1.70 A; A/B=282-682.
DR PDB; 6E3F; X-ray; 2.70 A; A=747-1241.
DR PDBsum; 6E36; -.
DR PDBsum; 6E3F; -.
DR AlphaFoldDB; Q9KW51; -.
DR SMR; Q9KW51; -.
DR STRING; 1338.A6J72_06540; -.
DR PRIDE; Q9KW51; -.
DR GeneID; 57845466; -.
DR OMA; APNSWYG; -.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-KW.
DR Gene3D; 2.60.530.10; -; 1.
DR InterPro; IPR026345; Adh_isopep-form_adh_dom.
DR InterPro; IPR041324; AgI/II_N.
DR InterPro; IPR032300; Antigen_C.
DR InterPro; IPR021197; Cross-wall-target_lipo_motif.
DR InterPro; IPR013574; Glucan-bd_C/Surface_Ag-I/II_V.
DR InterPro; IPR019931; LPXTG_anchor.
DR InterPro; IPR036234; SA_I/II_PAC_V_sf.
DR InterPro; IPR009578; Surface_Ag_rpt.
DR Pfam; PF18652; Adhesin_P1_N; 1.
DR Pfam; PF17998; AgI_II_C2; 1.
DR Pfam; PF16364; Antigen_C; 1.
DR Pfam; PF08363; GbpC; 1.
DR Pfam; PF00746; Gram_pos_anchor; 1.
DR Pfam; PF06696; Strep_SA_rep; 3.
DR SUPFAM; SSF74914; SSF74914; 1.
DR TIGRFAMs; TIGR04228; isopep_sspB_C2; 1.
DR TIGRFAMs; TIGR03726; strep_RK_lipo; 1.
DR PROSITE; PS51965; AG_I_II_AR; 2.
DR PROSITE; PS50847; GRAM_POS_ANCHORING; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Cell wall; Peptidoglycan-anchor; Repeat; Secreted; Signal.
FT SIGNAL 1..38
FT /evidence="ECO:0000255"
FT CHAIN 39..1280
FT /note="Probable cell-surface antigen I/II"
FT /id="PRO_0000379121"
FT PROPEP 1281..1310
FT /note="Removed by sortase"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00477"
FT /id="PRO_0000379122"
FT REPEAT 146..220
FT /note="Ag I/II A 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01310"
FT REPEAT 221..302
FT /note="Ag I/II A 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01310"
FT REGION 40..90
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 297..668
FT /note="V-region (lectin-like)"
FT /evidence="ECO:0000250"
FT REGION 330..349
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 663..736
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 1277..1281
FT /note="LPXTG sorting signal"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00477"
FT COMPBIAS 40..85
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 695..736
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 1280
FT /note="Pentaglycyl murein peptidoglycan amidated threonine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00477"
FT HELIX 286..328
FT /evidence="ECO:0007829|PDB:6E36"
FT TURN 329..331
FT /evidence="ECO:0007829|PDB:6E36"
FT STRAND 336..338
FT /evidence="ECO:0007829|PDB:6E36"
FT STRAND 353..357
FT /evidence="ECO:0007829|PDB:6E36"
FT STRAND 359..364
FT /evidence="ECO:0007829|PDB:6E36"
FT HELIX 366..374
FT /evidence="ECO:0007829|PDB:6E36"
FT HELIX 376..378
FT /evidence="ECO:0007829|PDB:6E36"
FT HELIX 379..382
FT /evidence="ECO:0007829|PDB:6E36"
FT TURN 383..385
FT /evidence="ECO:0007829|PDB:6E36"
FT HELIX 389..391
FT /evidence="ECO:0007829|PDB:6E36"
FT HELIX 394..397
FT /evidence="ECO:0007829|PDB:6E36"
FT STRAND 403..406
FT /evidence="ECO:0007829|PDB:6E36"
FT STRAND 410..412
FT /evidence="ECO:0007829|PDB:6E36"
FT HELIX 414..416
FT /evidence="ECO:0007829|PDB:6E36"
FT TURN 422..424
FT /evidence="ECO:0007829|PDB:6E36"
FT STRAND 430..437
FT /evidence="ECO:0007829|PDB:6E36"
FT STRAND 441..447
FT /evidence="ECO:0007829|PDB:6E36"
FT STRAND 457..468
FT /evidence="ECO:0007829|PDB:6E36"
FT STRAND 480..488
FT /evidence="ECO:0007829|PDB:6E36"
FT HELIX 489..491
FT /evidence="ECO:0007829|PDB:6E36"
FT STRAND 493..496
FT /evidence="ECO:0007829|PDB:6E36"
FT STRAND 503..505
FT /evidence="ECO:0007829|PDB:6E36"
FT STRAND 507..518
FT /evidence="ECO:0007829|PDB:6E36"
FT STRAND 526..533
FT /evidence="ECO:0007829|PDB:6E36"
FT STRAND 542..548
FT /evidence="ECO:0007829|PDB:6E36"
FT STRAND 550..554
FT /evidence="ECO:0007829|PDB:6E36"
FT STRAND 560..564
FT /evidence="ECO:0007829|PDB:6E36"
FT STRAND 567..570
FT /evidence="ECO:0007829|PDB:6E36"
FT HELIX 605..608
FT /evidence="ECO:0007829|PDB:6E36"
FT STRAND 610..617
FT /evidence="ECO:0007829|PDB:6E36"
FT STRAND 619..627
FT /evidence="ECO:0007829|PDB:6E36"
FT HELIX 628..630
FT /evidence="ECO:0007829|PDB:6E36"
FT TURN 636..638
FT /evidence="ECO:0007829|PDB:6E36"
FT STRAND 646..650
FT /evidence="ECO:0007829|PDB:6E36"
FT STRAND 759..764
FT /evidence="ECO:0007829|PDB:6E3F"
FT STRAND 781..788
FT /evidence="ECO:0007829|PDB:6E3F"
FT STRAND 801..806
FT /evidence="ECO:0007829|PDB:6E3F"
FT STRAND 811..813
FT /evidence="ECO:0007829|PDB:6E3F"
FT HELIX 815..821
FT /evidence="ECO:0007829|PDB:6E3F"
FT STRAND 825..830
FT /evidence="ECO:0007829|PDB:6E3F"
FT TURN 831..834
FT /evidence="ECO:0007829|PDB:6E3F"
FT STRAND 835..840
FT /evidence="ECO:0007829|PDB:6E3F"
FT HELIX 842..849
FT /evidence="ECO:0007829|PDB:6E3F"
FT STRAND 862..867
FT /evidence="ECO:0007829|PDB:6E3F"
FT STRAND 869..871
FT /evidence="ECO:0007829|PDB:6E3F"
FT STRAND 873..876
FT /evidence="ECO:0007829|PDB:6E3F"
FT STRAND 879..882
FT /evidence="ECO:0007829|PDB:6E3F"
FT STRAND 887..889
FT /evidence="ECO:0007829|PDB:6E3F"
FT STRAND 893..896
FT /evidence="ECO:0007829|PDB:6E3F"
FT STRAND 915..917
FT /evidence="ECO:0007829|PDB:6E3F"
FT STRAND 936..943
FT /evidence="ECO:0007829|PDB:6E3F"
FT HELIX 946..948
FT /evidence="ECO:0007829|PDB:6E3F"
FT HELIX 955..958
FT /evidence="ECO:0007829|PDB:6E3F"
FT STRAND 962..967
FT /evidence="ECO:0007829|PDB:6E3F"
FT TURN 970..972
FT /evidence="ECO:0007829|PDB:6E3F"
FT STRAND 973..975
FT /evidence="ECO:0007829|PDB:6E3F"
FT HELIX 977..979
FT /evidence="ECO:0007829|PDB:6E3F"
FT STRAND 981..984
FT /evidence="ECO:0007829|PDB:6E3F"
FT STRAND 992..999
FT /evidence="ECO:0007829|PDB:6E3F"
FT TURN 1001..1003
FT /evidence="ECO:0007829|PDB:6E3F"
FT HELIX 1006..1015
FT /evidence="ECO:0007829|PDB:6E3F"
FT STRAND 1023..1030
FT /evidence="ECO:0007829|PDB:6E3F"
FT HELIX 1031..1037
FT /evidence="ECO:0007829|PDB:6E3F"
FT TURN 1038..1042
FT /evidence="ECO:0007829|PDB:6E3F"
FT STRAND 1045..1053
FT /evidence="ECO:0007829|PDB:6E3F"
FT HELIX 1056..1060
FT /evidence="ECO:0007829|PDB:6E3F"
FT STRAND 1062..1072
FT /evidence="ECO:0007829|PDB:6E3F"
FT STRAND 1075..1078
FT /evidence="ECO:0007829|PDB:6E3F"
FT STRAND 1082..1086
FT /evidence="ECO:0007829|PDB:6E3F"
FT STRAND 1092..1097
FT /evidence="ECO:0007829|PDB:6E3F"
FT STRAND 1117..1123
FT /evidence="ECO:0007829|PDB:6E3F"
FT STRAND 1129..1133
FT /evidence="ECO:0007829|PDB:6E3F"
FT STRAND 1138..1144
FT /evidence="ECO:0007829|PDB:6E3F"
FT TURN 1146..1148
FT /evidence="ECO:0007829|PDB:6E3F"
FT STRAND 1149..1161
FT /evidence="ECO:0007829|PDB:6E3F"
FT STRAND 1163..1165
FT /evidence="ECO:0007829|PDB:6E3F"
FT HELIX 1178..1180
FT /evidence="ECO:0007829|PDB:6E3F"
FT STRAND 1181..1186
FT /evidence="ECO:0007829|PDB:6E3F"
FT TURN 1187..1190
FT /evidence="ECO:0007829|PDB:6E3F"
FT STRAND 1191..1196
FT /evidence="ECO:0007829|PDB:6E3F"
FT HELIX 1198..1202
FT /evidence="ECO:0007829|PDB:6E3F"
FT STRAND 1212..1220
FT /evidence="ECO:0007829|PDB:6E3F"
FT STRAND 1222..1224
FT /evidence="ECO:0007829|PDB:6E3F"
FT STRAND 1228..1234
FT /evidence="ECO:0007829|PDB:6E3F"
FT STRAND 1237..1240
FT /evidence="ECO:0007829|PDB:6E3F"
SQ SEQUENCE 1310 AA; 143090 MW; 0021781F5D4DB793 CRC64;
MKKRKEVFGF RKSKVAKTLC GAVLGAALLA MVDQQVAAEE TTKSNSTTNV AVTATGNPAT
NLPEAQGSAS KEAEQSQNQA GETNGSIPVE VPKTDLDQAA KEAKSAGVNV VQDADVNKGT
VKTAGEAAQK ETEIKEDYTK QAEDIKKTTD QYKSDVAAHE AEVAKIKAKN QATKEQYEKD
MAAHKAEVER INAANAASKA AYETKLAQYQ AELKRVQEAN ATNEANYQAK LTAYKTELAR
VQKANADAKA AYEAAVAANN AANAALTAEN TAIKKRNADA KADYEAKLAK YQADLAKYQK
DLAEYPQKLK EYNEEQAKIK EALKKLEQDK NKDGHLTEPS AQSLVYDSEP DAKLSLTTED
GTLLKSSVVD EAFSKSTSKA KYDQKILQLD DLDIRGLEKA DSATSTVELY GNIGNKSTWT
TNVGNNTEVK WGSVLLKRGQ SVTATYTNLQ KTYYNGKKVS KIVYKYTVDK DSKFQNPSGN
VWLGVFSDPT LGVFASAYTG QVEKDTSIFI KNEFTFYDEN DQPINFDNAL LSVASLNREN
NSIEMAKDYT GKFVRISGSS IDEKDGKIYA TKTLNFKKGQ GGSRWTMYPN GQEGSGWDSS
DAPNSWYGAG AVKISGQHNS ITLGAISATL VVPSDSVMAV ETGKKPNIWY SLNGKIRAVN
VPKITKENPT PPVEPTAPQA PTYEVEKPLN PAPVAPNYEK EPTPPTPTPD QPEPNKPVEP
TYEAIPTPPT DPVYQNLPTP PAVPTVHFRY YKLAVQPQVN KEIKNNNDVD IDKTLVAKQS
VVKFQLKTAD LPAGRAKTTS FVLVDPLPSG YQFNLEATKA ASPGFDVSYD KATNTVTFKA
TAATLATFNA DLTKSVATIY PTVVGQVLND GATYKNNFTL TVNDAYGIKS NVVRVTTPGK
PNDPDNPNNN YIKPTKVNKN ENGVVIDGKT VLAGSTNYYE LTWDLDQYKN DRSSADTIQK
GFYYVDDYPE EALELRQDLV KITDANGNKV TGVSVDHYTS LEAAPQEVRD VLSKAGIRPK
GAFQIFRADN PREFYDTYVK NGIDLKIVSP MVVKKQMGQT GGSYENQAYQ IDFGNGYASN
IVINNVPKIN PKKDVTLTLD PADTNNVDGQ TIPLNTVFNY RLIGGIIPAD HSEELFEYNF
YDDYDQTGDR YTGQYKVFAK VDITLKDGSV IKSGTDLTQH TTAEVDATKG ALTIKFKEDF
LRSVSIDSAF QAESYIQMKR IAAGTFENTY VNTVNKVAYA SNTVRTTTPQ APRSEVPKRP
TPVSYLTNKL SSAPATLPQT GTTDSSYMPY LGVIALLSVL GLGQLKRNEK
