ID   ASOL_BRANA              Reviewed;         555 AA.
AC   Q00624;
DT   01-DEC-1992, integrated into UniProtKB/Swiss-Prot.
DT   01-DEC-1992, sequence version 1.
DT   25-MAY-2022, entry version 92.
DE   RecName: Full=L-ascorbate oxidase homolog;
DE            EC=1.10.3.-;
DE   Flags: Precursor;
GN   Name=Bp10;
OS   Brassica napus (Rape).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Brassiceae; Brassica.
OX   NCBI_TaxID=3708;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND TISSUE SPECIFICITY.
RC   STRAIN=cv. Westar;
RX   PubMed=1303799; DOI=10.1111/j.1365-313x.1992.00331.x;
RA   Albani D., Sardana R., Robert L.S., Altosaar I., Arnison P.G.,
RA   Fabijanski S.F.;
RT   "A Brassica napus gene family which shows sequence similarity to ascorbate
RT   oxidase is expressed in developing pollen. Molecular characterization and
RT   analysis of promoter activity in transgenic tobacco plants.";
RL   Plant J. 2:331-342(1992).
CC   -!- FUNCTION: Probable oxidase that may be involved in pollen tube growth.
CC       {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000305}.
CC   -!- TISSUE SPECIFICITY: Maximal expression in early binucleate microspores;
CC       declines considerably in mature trinucleate pollen.
CC       {ECO:0000269|PubMed:1303799}.
CC   -!- SIMILARITY: Belongs to the multicopper oxidase family. {ECO:0000305}.
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DR   EMBL; X64257; CAA45554.1; -; Genomic_DNA.
DR   PIR; S23763; S23763.
DR   RefSeq; XP_013749860.1; XM_013894406.1.
DR   AlphaFoldDB; Q00624; -.
DR   SMR; Q00624; -.
DR   EnsemblPlants; CDY43995; CDY43995; GSBRNA2T00079392001.
DR   GeneID; 106452325; -.
DR   Gramene; CDY43995; CDY43995; GSBRNA2T00079392001.
DR   KEGG; bna:106452325; -.
DR   OMA; MTHRNFI; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0005507; F:copper ion binding; IEA:InterPro.
DR   GO; GO:0016491; F:oxidoreductase activity; IEA:UniProtKB-KW.
DR   CDD; cd13872; CuRO_2_AAO_like_1; 1.
DR   CDD; cd13894; CuRO_3_AAO_like_1; 1.
DR   Gene3D; 2.60.40.420; -; 3.
DR   InterPro; IPR001117; Cu-oxidase.
DR   InterPro; IPR011706; Cu-oxidase_C.
DR   InterPro; IPR045087; Cu-oxidase_fam.
DR   InterPro; IPR011707; Cu-oxidase_N.
DR   InterPro; IPR008972; Cupredoxin.
DR   InterPro; IPR034271; CuRO_2_AO-like.
DR   InterPro; IPR034275; CuRO_3_AO-like.
DR   PANTHER; PTHR11709; PTHR11709; 1.
DR   Pfam; PF00394; Cu-oxidase; 1.
DR   Pfam; PF07731; Cu-oxidase_2; 1.
DR   Pfam; PF07732; Cu-oxidase_3; 1.
DR   SUPFAM; SSF49503; SSF49503; 3.
PE   2: Evidence at transcript level;
KW   Disulfide bond; Germination; Glycoprotein; Metal-binding; Oxidoreductase;
KW   Repeat; Secreted; Signal.
FT   SIGNAL          1..23
FT                   /evidence="ECO:0000255"
FT   CHAIN           24..555
FT                   /note="L-ascorbate oxidase homolog"
FT                   /id="PRO_0000002905"
FT   DOMAIN          25..145
FT                   /note="Plastocyanin-like 1"
FT   DOMAIN          158..301
FT                   /note="Plastocyanin-like 2"
FT   DOMAIN          345..524
FT                   /note="Plastocyanin-like 3"
FT   CARBOHYD        33
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        61
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        110
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        330
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        350
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        422
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   DISULFID        103..539
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   555 AA;  62131 MW;  5BF23C9D73EA6C6A CRC64;
     MRGVKLLAAC LYLAAAATVV VHAEDPYFHH VWNVTYGTAS PLGVPQQVIL INGQFPGPNI
     NSTSNNNVII NVFNNLDEPF LLTWNGIQHR KNCWQDGTPG TMCPIMPGTN YTYHFQPKDQ
     IGSYFYYPTT GMHRAAGGYG GLRVNSRLLI PVPYADPEDD YTVLIGDWYT KSHTQLKKFL
     DGGRTIGRPD GIVINGKSGK GDGSDAPLFT LKPGKTYRVR ICNVGVKTSI NFRIQNHKMK
     LVEMEGSHVL QNDYDSLDVH VGQCFGTIVT ANQEPKDYYM VASSRFLKTV ITTTGLLRYE
     GGKGPASSQL PAGPVGWAWS LNQFRSFRWN LTASAARPNP QGSYHYGKIN ITRTIKLVNT
     QGKVDGKLRF ALNGVSHTEP ETPLKLAEYF GISDKVFKYD TITDDPTPEQ IKNIKIEPNV
     LNITHRTFVE VVFENHEKSV QSWHLDGYSF FSVAVEPGTW TPEKRKNYNL LDAVSRHTVQ
     VYPKCWAAIL LTFDNCGMWN VRSENTERRY LGQQLYASVL SPEKSLRDEY NMPETSLQCG
     LVKNTPKPVN PYAGA