ASOL_TOBAC
ID ASOL_TOBAC Reviewed; 554 AA.
AC P29162;
DT 01-DEC-1992, integrated into UniProtKB/Swiss-Prot.
DT 01-DEC-1992, sequence version 1.
DT 25-MAY-2022, entry version 95.
DE RecName: Full=L-ascorbate oxidase homolog;
DE EC=1.10.3.-;
DE AltName: Full=Pollen-specific protein NTP303;
DE Flags: Precursor;
OS Nicotiana tabacum (Common tobacco).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC asterids; lamiids; Solanales; Solanaceae; Nicotianoideae; Nicotianeae;
OC Nicotiana.
OX NCBI_TaxID=4097;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL
RP STAGE, AND INDUCTION.
RC STRAIN=cv. Petit Havana SR1; TISSUE=Pollen;
RX PubMed=1600146; DOI=10.1007/bf00047713;
RA Weterings K., Reijnen W., van Aarssen R., Kortstee A., Spijkers J.,
RA van Herpen M., Schrauwen J., Wullems G.;
RT "Characterization of a pollen-specific cDNA clone from Nicotiana tabacum
RT expressed during microgametogenesis and germination.";
RL Plant Mol. Biol. 18:1101-1111(1992).
CC -!- FUNCTION: Probable oxidoreductase that may be involved in pollen tube
CC growth. {ECO:0000269|PubMed:1600146}.
CC -!- SUBCELLULAR LOCATION: Secreted, extracellular space {ECO:0000305}.
CC -!- TISSUE SPECIFICITY: Pollen. {ECO:0000269|PubMed:1600146}.
CC -!- DEVELOPMENTAL STAGE: Appears after the first haploid mitosis and are
CC expressed during microgametogenesis, germination and tube growth.
CC {ECO:0000269|PubMed:1600146}.
CC -!- INDUCTION: Expression regulated by the haploid gametophyte itself.
CC {ECO:0000269|PubMed:1600146}.
CC -!- SIMILARITY: Belongs to the multicopper oxidase family. {ECO:0000305}.
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DR EMBL; X61146; CAA43454.1; -; mRNA.
DR PIR; S22495; S22495.
DR RefSeq; NP_001311861.1; NM_001324932.1.
DR AlphaFoldDB; P29162; -.
DR SMR; P29162; -.
DR STRING; 4097.P29162; -.
DR ProMEX; P29162; -.
DR GeneID; 107766889; -.
DR KEGG; nta:107766889; -.
DR OMA; ENHENTM; -.
DR Proteomes; UP000084051; Unplaced.
DR GO; GO:0005615; C:extracellular space; IEA:UniProtKB-SubCell.
DR GO; GO:0005507; F:copper ion binding; IEA:InterPro.
DR GO; GO:0016491; F:oxidoreductase activity; IBA:GO_Central.
DR CDD; cd13846; CuRO_1_AAO_like_1; 1.
DR CDD; cd13872; CuRO_2_AAO_like_1; 1.
DR Gene3D; 2.60.40.420; -; 3.
DR InterPro; IPR001117; Cu-oxidase.
DR InterPro; IPR011706; Cu-oxidase_C.
DR InterPro; IPR045087; Cu-oxidase_fam.
DR InterPro; IPR011707; Cu-oxidase_N.
DR InterPro; IPR008972; Cupredoxin.
DR InterPro; IPR034273; CuRO_1_AAO-like.
DR InterPro; IPR034271; CuRO_2_AO-like.
DR PANTHER; PTHR11709; PTHR11709; 1.
DR Pfam; PF00394; Cu-oxidase; 1.
DR Pfam; PF07731; Cu-oxidase_2; 1.
DR Pfam; PF07732; Cu-oxidase_3; 1.
DR SUPFAM; SSF49503; SSF49503; 3.
PE 2: Evidence at transcript level;
KW Disulfide bond; Germination; Glycoprotein; Metal-binding; Oxidoreductase;
KW Reference proteome; Repeat; Secreted; Signal.
FT SIGNAL 1..21
FT /evidence="ECO:0000255"
FT CHAIN 22..554
FT /note="L-ascorbate oxidase homolog"
FT /id="PRO_0000002909"
FT DOMAIN 22..143
FT /note="Plastocyanin-like 1"
FT DOMAIN 196..296
FT /note="Plastocyanin-like 2"
FT DOMAIN 411..521
FT /note="Plastocyanin-like 3"
FT CARBOHYD 31
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 59
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 108
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 332
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 352
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 423
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT DISULFID 101..540
FT /evidence="ECO:0000255"
SQ SEQUENCE 554 AA; 62034 MW; 9D38DAB1F52E2F85 CRC64;
MGSGKVTFVA LLLCLSVGVI AEDPYLYFNW NVTYGTIAPL GVPQQGILIN GQFPGPRINC
TSNNNIVVNV FNNLDEPFLF TWNGVQHRKN SWQDGTPGTM CPIMPGQNFT YRFQVKDQIG
SYSYFPTTAL HRAAGGYGAL NVHSRALIPV PFDNPADEYN VFVGDWYNKG HKTLKKILDG
GRTIGRPDGI IINGKSAKVG EAKEPLFTME AGKTYRYRFC NLGMRSSVNI RFQGHPMKLV
ELEGSHTVQN IYDSLDLHVG QCLSVLVTAD QEPKDYYLVV SSRFLKQALS SVAIIRYANG
KGPASPELPT PPPENTEGIA WSMNQFRSFR WNLTASAARP NPQGSYHYGQ INITRTIKIF
NSMSQVGGKL RYGLNGISHT NGETPLKLVE YFGATNKAFK YDLMADEAPA DPSKLTIATN
VKNATYRNFV EIIFENHEKT IRTYHLDGYS FFAVAVEPGR WSPEKRKNYN LVDGLSRNNI
QVYPNSWAAI MLTFDNAGMW NLRSEMWEKT YLGEQLYFSV LSPSRSLRDE YNIPDNHPLC
GIVKGLSMPA PYKA