PAT1H_ARATH
ID PAT1H_ARATH Reviewed; 793 AA.
AC Q0WPK4; O64542; Q56X08;
DT 31-JAN-2018, integrated into UniProtKB/Swiss-Prot.
DT 05-SEP-2006, sequence version 1.
DT 25-MAY-2022, entry version 111.
DE RecName: Full=Protein PAT1 homolog {ECO:0000305};
DE Short=AtPAT1 {ECO:0000303|PubMed:25603932};
GN Name=PAT1 {ECO:0000303|PubMed:25603932};
GN OrderedLocusNames=At1g79090 {ECO:0000312|Araport:AT1G79090};
GN ORFNames=YUP8H12R.29 {ECO:0000312|EMBL:AAC17049.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=19423640; DOI=10.1093/dnares/dsp009;
RA Iida K., Fukami-Kobayashi K., Toyoda A., Sakaki Y., Kobayashi M., Seki M.,
RA Shinozaki K.;
RT "Analysis of multiple occurrences of alternative splicing events in
RT Arabidopsis thaliana using novel sequenced full-length cDNAs.";
RL DNA Res. 16:155-164(2009).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC STRAIN=cv. Columbia;
RX PubMed=19245862; DOI=10.1016/j.jprot.2009.02.004;
RA Jones A.M.E., MacLean D., Studholme D.J., Serna-Sanz A., Andreasson E.,
RA Rathjen J.P., Peck S.C.;
RT "Phosphoproteomic analysis of nuclei-enriched fractions from Arabidopsis
RT thaliana.";
RL J. Proteomics 72:439-451(2009).
RN [6]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-208, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=19376835; DOI=10.1104/pp.109.138677;
RA Reiland S., Messerli G., Baerenfaller K., Gerrits B., Endler A.,
RA Grossmann J., Gruissem W., Baginsky S.;
RT "Large-scale Arabidopsis phosphoproteome profiling reveals novel
RT chloroplast kinase substrates and phosphorylation networks.";
RL Plant Physiol. 150:889-903(2009).
RN [7]
RP FUNCTION, INTERACTION WITH MPK4 AND SUMM2, SUBCELLULAR LOCATION, INDUCTION
RP BY FLAGELLIN, PHOSPHORYLATION AT SER-200; SER-208; SER-342 AND SER-343,
RP MUTAGENESIS OF SER-208, AND DISRUPTION PHENOTYPE.
RX PubMed=25603932; DOI=10.15252/embj.201488645;
RA Roux M.E., Rasmussen M.W., Palma K., Lolle S., Regue A.M., Bethke G.,
RA Glazebrook J., Zhang W., Sieburth L., Larsen M.R., Mundy J., Petersen M.;
RT "The mRNA decay factor PAT1 functions in a pathway including MAP kinase 4
RT and immune receptor SUMM2.";
RL EMBO J. 34:593-608(2015).
CC -!- FUNCTION: Activator of mRNA decapping. Involved in mRNA decay via
CC decapping. Involved in disease resistance in response to biotrophic and
CC necrotrophic pathogens. Is part of a signaling pathway including MPK4
CC and the disease resistance protein SUMM2.
CC {ECO:0000269|PubMed:25603932}.
CC -!- SUBUNIT: Interacts with MPK4 and SUMM2. {ECO:0000269|PubMed:25603932}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm, P-body {ECO:0000269|PubMed:25603932}.
CC -!- INDUCTION: Accumulates in response to flg22 (at protein levels).
CC {ECO:0000269|PubMed:25603932}.
CC -!- PTM: Phosphorylated at Ser-208 by MPK4 upon flg22 elicitation.
CC Phosphorylated at Ser-200, Ser-342 and Ser-343 upon flg22 elicitation.
CC {ECO:0000269|PubMed:25603932}.
CC -!- DISRUPTION PHENOTYPE: Serrated leaf and reduced plant size.
CC Constitutive expression of the defense-related gene PR1 and enhanced
CC resistance to the bacterial pathogen Pseudomonas syringae pv tomato
CC strain DC3000. {ECO:0000269|PubMed:25603932}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAC17049.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AC002986; AAC17049.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002684; AEE36202.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE36203.1; -; Genomic_DNA.
DR EMBL; CP002684; ANM57835.1; -; Genomic_DNA.
DR EMBL; AK318693; BAH56808.1; -; mRNA.
DR EMBL; AK229063; BAF00945.1; -; mRNA.
DR EMBL; AK221871; BAD94175.1; -; mRNA.
DR PIR; T01046; T01046.
DR RefSeq; NP_001320315.1; NM_001334869.1.
DR RefSeq; NP_565199.1; NM_106560.4.
DR RefSeq; NP_849904.1; NM_179573.4.
DR AlphaFoldDB; Q0WPK4; -.
DR ComplexPortal; CPX-1308; LSM1-7-PAT1 complex, variant LSM1A-LSM3A-LSM6A-PAT1.
DR ComplexPortal; CPX-1345; LSM1-7-PAT1 complex, variant LSM1A-LSM3B-LSM6B-PAT1.
DR ComplexPortal; CPX-1346; LSM1-7-PAT1 complex, variant LSM1A-LSM3B-LSM6A-PAT1.
DR ComplexPortal; CPX-1347; LSM1-7-PAT1 complex, variant LSM1B-LSM3A-LSM6A-PAT1.
DR ComplexPortal; CPX-1348; LSM1-7-PAT1 complex, variant LSM1B-LSM3B-LSM6A-PAT1.
DR ComplexPortal; CPX-1349; LSM1-7-PAT1 complex, variant LSM1B-LSM3B-LSM6B-PAT1.
DR ComplexPortal; CPX-1350; LSM1-7-PAT1 complex, variant LSM1B-LSM3A-LSM6B-PAT1.
DR ComplexPortal; CPX-1351; LSM1-7-PAT1 complex, variant LSM1A-LSM3A-LSM6B-PAT1.
DR STRING; 3702.AT1G79090.2; -.
DR iPTMnet; Q0WPK4; -.
DR PaxDb; Q0WPK4; -.
DR PRIDE; Q0WPK4; -.
DR ProteomicsDB; 236325; -.
DR EnsemblPlants; AT1G79090.1; AT1G79090.1; AT1G79090.
DR EnsemblPlants; AT1G79090.2; AT1G79090.2; AT1G79090.
DR EnsemblPlants; AT1G79090.3; AT1G79090.3; AT1G79090.
DR GeneID; 844250; -.
DR Gramene; AT1G79090.1; AT1G79090.1; AT1G79090.
DR Gramene; AT1G79090.2; AT1G79090.2; AT1G79090.
DR Gramene; AT1G79090.3; AT1G79090.3; AT1G79090.
DR KEGG; ath:AT1G79090; -.
DR Araport; AT1G79090; -.
DR TAIR; locus:2207400; AT1G79090.
DR eggNOG; ENOG502QQ60; Eukaryota.
DR HOGENOM; CLU_021130_0_0_1; -.
DR InParanoid; Q0WPK4; -.
DR OMA; QHSSQMM; -.
DR OrthoDB; 369950at2759; -.
DR PhylomeDB; Q0WPK4; -.
DR PRO; PR:Q0WPK4; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q0WPK4; baseline and differential.
DR GO; GO:1990726; C:Lsm1-7-Pat1 complex; IC:ComplexPortal.
DR GO; GO:0000932; C:P-body; IDA:TAIR.
DR GO; GO:0003729; F:mRNA binding; IDA:TAIR.
DR GO; GO:0003723; F:RNA binding; IBA:GO_Central.
DR GO; GO:0000290; P:deadenylation-dependent decapping of nuclear-transcribed mRNA; IMP:TAIR.
DR GO; GO:0045087; P:innate immune response; IMP:TAIR.
DR GO; GO:0006397; P:mRNA processing; IEA:UniProtKB-KW.
DR GO; GO:0033962; P:P-body assembly; IBA:GO_Central.
DR InterPro; IPR039900; Pat1-like.
DR PANTHER; PTHR21551; PTHR21551; 1.
PE 1: Evidence at protein level;
KW Cytoplasm; mRNA processing; Phosphoprotein; Plant defense;
KW Reference proteome.
FT CHAIN 1..793
FT /note="Protein PAT1 homolog"
FT /id="PRO_0000442788"
FT REGION 203..256
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 291..312
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 476..501
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 222..256
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 200
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:25603932"
FT MOD_RES 208
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:25603932,
FT ECO:0007744|PubMed:19376835"
FT MOD_RES 342
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:25603932"
FT MOD_RES 343
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:25603932"
FT MUTAGEN 208
FT /note="S->A: Abolishes phosphorylation by MPK4."
FT /evidence="ECO:0000269|PubMed:25603932"
FT CONFLICT 527
FT /note="D -> E (in Ref. 4; BAD94175)"
FT /evidence="ECO:0000305"
FT CONFLICT 711
FT /note="N -> K (in Ref. 4; BAD94175)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 793 AA; 88840 MW; 8C70A4DFD7A9EA6E CRC64;
MDAFGIGSSL NQAPVTQDLK KFGDNSTGNT MFDASQYAFF GNDVVEEVEL GGLEEEDEIL
SFTGIAEDFS FDKEEVGDSR LLSDVDDLAS TFSKLNREPD VYSNTGPITD RRSSQNSLAA
EWTHGEELPN WYGRQILDSD AIKDDKVWSA QPFSSLDRVE QRIPDRTKLY PEPQRQLHQD
HNQQQFSSEP ILVPKSSFVS YPPPGSISPD QRLGHPNIPY QSGGPQMGSP NFSPFPNLQP
QLPSMHHGSP QHTGNRPQFR PALPLNNLPP AQWMNRQNMH PGDSSGIMNN AMLQQPPHQN
GLMPPQMQGS QNRLPHPMQP PLGHMPGMQP QLFNSHLSRS SSSGNYDGML GFGDLREVRP
GSGHGNRQNV RFPQQGFDAG VQRRYPFRSK YMSAGEIENI LRMQLVATHS NDPYVDDYYH
QACLAKKSAG AKLKHHFCPN HLRDLQQRAR SNNEPHAFLQ VEALGRVPFS SIRRPRPLLE
VDPPNSAKFG NAEHKPTDKP LDQEPMLAAR VYIEDGLCLL LEVDDIDRFL EFNQLQDGGH
QLKQRRQALL QSLAVSLQLG DPLAKNGQSQ SLDDFLFLRV ISLPKGRKLL IRYLQLIFPG
SDLMRIVCMA IFRHLRSLFG VLSSDPDIIK TTNKLATVIN LCIQNMELGP VSTCLAAVSC
SSEQAPLRPL GSPVGDGAST VLKSILDRAS ELIRANNFNN AGIALWRASF NEFFNMLMRY
CISKYDSIMQ SLQLPPHFAT EISEEAAKAI VREMPIELLR SSFPHIDEQQ KRILMEFLKR
SMLGSQKTEP VLS
