PATA_ASPCL
ID PATA_ASPCL Reviewed; 247 AA.
AC A1CFK8;
DT 07-SEP-2016, integrated into UniProtKB/Swiss-Prot.
DT 23-JAN-2007, sequence version 1.
DT 03-AUG-2022, entry version 54.
DE RecName: Full=Acetate transporter protein patA {ECO:0000303|PubMed:19383676};
DE AltName: Full=Patulin synthesis protein A {ECO:0000303|PubMed:19383676};
GN Name=patA {ECO:0000303|PubMed:19383676}; ORFNames=ACLA_093560;
OS Aspergillus clavatus (strain ATCC 1007 / CBS 513.65 / DSM 816 / NCTC 3887 /
OS NRRL 1 / QM 1276 / 107).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Eurotiomycetes;
OC Eurotiomycetidae; Eurotiales; Aspergillaceae; Aspergillus;
OC Aspergillus subgen. Fumigati.
OX NCBI_TaxID=344612;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 1007 / CBS 513.65 / DSM 816 / NCTC 3887 / NRRL 1;
RX PubMed=18404212; DOI=10.1371/journal.pgen.1000046;
RA Fedorova N.D., Khaldi N., Joardar V.S., Maiti R., Amedeo P., Anderson M.J.,
RA Crabtree J., Silva J.C., Badger J.H., Albarraq A., Angiuoli S., Bussey H.,
RA Bowyer P., Cotty P.J., Dyer P.S., Egan A., Galens K., Fraser-Liggett C.M.,
RA Haas B.J., Inman J.M., Kent R., Lemieux S., Malavazi I., Orvis J.,
RA Roemer T., Ronning C.M., Sundaram J.P., Sutton G., Turner G., Venter J.C.,
RA White O.R., Whitty B.R., Youngman P., Wolfe K.H., Goldman G.H.,
RA Wortman J.R., Jiang B., Denning D.W., Nierman W.C.;
RT "Genomic islands in the pathogenic filamentous fungus Aspergillus
RT fumigatus.";
RL PLoS Genet. 4:E1000046-E1000046(2008).
RN [2]
RP BIOTECHNOLOGY.
RX PubMed=15082620; DOI=10.1093/ije/dyh028;
RG Patulin Clinical Trials Committee, Medical Research Council;
RT "Clinical trial of patulin in the common cold. 1944.";
RL Int. J. Epidemiol. 33:243-246(2004).
RN [3]
RP FUNCTION.
RX PubMed=19383676; DOI=10.1099/mic.0.024836-0;
RA Artigot M.P., Loiseau N., Laffitte J., Mas-Reguieg L., Tadrist S.,
RA Oswald I.P., Puel O.;
RT "Molecular cloning and functional characterization of two CYP619 cytochrome
RT P450s involved in biosynthesis of patulin in Aspergillus clavatus.";
RL Microbiology 155:1738-1747(2009).
RN [4]
RP BIOTECHNOLOGY.
RX PubMed=22222931; DOI=10.1016/j.fct.2011.12.022;
RA de Melo F.T., de Oliveira I.M., Greggio S., Dacosta J.C., Guecheva T.N.,
RA Saffi J., Henriques J.A., Rosa R.M.;
RT "DNA damage in organs of mice treated acutely with patulin, a known
RT mycotoxin.";
RL Food Chem. Toxicol. 50:3548-3555(2012).
RN [5]
RP FUNCTION.
RX PubMed=24334092; DOI=10.1016/j.ijfoodmicro.2013.11.020;
RA Snini S.P., Tadrist S., Laffitte J., Jamin E.L., Oswald I.P., Puel O.;
RT "The gene PatG involved in the biosynthesis pathway of patulin, a food-
RT borne mycotoxin, encodes a 6-methylsalicylic acid decarboxylase.";
RL Int. J. Food Microbiol. 171:77-83(2014).
RN [6]
RP BIOTECHNOLOGY.
RX PubMed=26619846; DOI=10.1007/s13277-015-4474-z;
RA Boussabbeh M., Ben Salem I., Rjiba-Touati K., Bouyahya C., Neffati F.,
RA Najjar M.F., Bacha H., Abid-Essefi S.;
RT "The potential effect of patulin on mice bearing melanoma cells: an anti-
RT tumour or carcinogenic effect?";
RL Tumor Biol. 37:6285-6295(2016).
CC -!- FUNCTION: Acetate transporter protein; part of the gene cluster that
CC mediates the biosynthesis of patulin, an acetate-derived tetraketide
CC mycotoxin produced by several fungal species that shows antimicrobial
CC properties against several bacteria (PubMed:19383676). May be involved
CC in the uptake of acetate, a substrate for the synthesis of 6-
CC methylsalicylic acid by the polyketide synthase patK (PubMed:19383676).
CC {ECO:0000305|PubMed:19383676}.
CC -!- PATHWAY: Mycotoxin biosynthesis; patulin biosynthesis.
CC {ECO:0000305|PubMed:19383676}.
CC -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane
CC {ECO:0000250|UniProtKB:A0A075TRL0}; Multi-pass membrane protein
CC {ECO:0000250|UniProtKB:A0A075TRL0}.
CC -!- BIOTECHNOLOGY: Patulin was originally used as an antibiotic and
CC specifically trialed to be used against the common cold, but it is no
CC longer used for that purpose since it has been shown to induce
CC immunological, neurological and gastrointestinal effects
CC (PubMed:15082620). Genotoxic effects of patulin with dose-dependent
CC increase in DNA strand breaks in brain, liver and kidneys have been
CC detected in mice (PubMed:22222931). However, more recently, it has been
CC proposed that patulin might also have anti-tumor properties
CC (PubMed:26619846). {ECO:0000269|PubMed:15082620,
CC ECO:0000269|PubMed:22222931, ECO:0000269|PubMed:26619846}.
CC -!- SIMILARITY: Belongs to the acetate uptake transporter (AceTr) (TC
CC 2.A.96) family. {ECO:0000305}.
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DR EMBL; DS027052; EAW11657.1; -; Genomic_DNA.
DR RefSeq; XP_001273083.1; XM_001273082.1.
DR AlphaFoldDB; A1CFK8; -.
DR SMR; A1CFK8; -.
DR EnsemblFungi; EAW11657; EAW11657; ACLA_093560.
DR GeneID; 4704863; -.
DR KEGG; act:ACLA_093560; -.
DR VEuPathDB; FungiDB:ACLA_093560; -.
DR eggNOG; ENOG502SI7M; Eukaryota.
DR HOGENOM; CLU_051062_2_1_1; -.
DR OMA; LGYYTVA; -.
DR OrthoDB; 1117364at2759; -.
DR UniPathway; UPA00918; -.
DR Proteomes; UP000006701; Unassembled WGS sequence.
DR GO; GO:0005789; C:endoplasmic reticulum membrane; ISS:GO_Central.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0006811; P:ion transport; IEA:UniProtKB-KW.
DR GO; GO:0140723; P:patulin biosynthetic process; ISS:GO_Central.
DR InterPro; IPR000791; Gpr1/Fun34/SatP.
DR Pfam; PF01184; Gpr1_Fun34_YaaH; 1.
PE 1: Evidence at protein level;
KW Endoplasmic reticulum; Glycoprotein; Ion transport; Membrane;
KW Reference proteome; Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..247
FT /note="Acetate transporter protein patA"
FT /id="PRO_0000437112"
FT TRANSMEM 37..57
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 71..91
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 106..126
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 141..161
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 169..189
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 202..222
FT /note="Helical"
FT /evidence="ECO:0000255"
FT CARBOHYD 20
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
SQ SEQUENCE 247 AA; 26173 MW; 1493A5459B7CBA61 CRC64;
MSNVKTAMDT SNEESQLSYN ASTSSSCAAG KEHATTPPIT PIGSPTALGM GAFAIAFTTL
SMSLMEWRGA AITNAYIGNC FFTAGLGLVL VAQWELVRGN SYGHTVFGGF GLFNLAFGAI
NAPAFGVADA FKDDPAALSN ALGYFLLVWG VFVLFFTIAA MPMNLVYTAM LGTSQITYTL
LAASYFAMAD EKATAGVGLK KAAGAFGFVS GLLAWYVVAH LMCQDALFFS FPLGDTSRLY
ARLRRNR
