PATB1_SOLTU
ID PATB1_SOLTU Reviewed; 386 AA.
AC P15476; Q41467;
DT 01-APR-1990, integrated into UniProtKB/Swiss-Prot.
DT 24-JUL-2007, sequence version 2.
DT 03-AUG-2022, entry version 80.
DE RecName: Full=Patatin-B1;
DE EC=3.1.1.-;
DE Flags: Precursor;
GN Name=PATB1;
OS Solanum tuberosum (Potato).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC asterids; lamiids; Solanales; Solanaceae; Solanoideae; Solaneae; Solanum.
OX NCBI_TaxID=4113;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=cv. Bintje;
RX AGRICOLA=IND92000050; DOI=10.1007/BF00027383;
RA Stiekema W.J., Heidekamp F., Dirkse W.G., van Beckum J., de Haan P.,
RA ten Bosch C., Louwerse J.D.;
RT "Molecular cloning and analysis of four potato tuber mRNAs.";
RL Plant Mol. Biol. 11:255-269(1988).
CC -!- FUNCTION: Probable lipolytic acyl hydrolase (LAH), an activity which is
CC thought to be involved in the response of tubers to pathogens.
CC -!- SUBCELLULAR LOCATION: Vacuole {ECO:0000250}.
CC -!- DOMAIN: The nitrogen atoms of the two glycine residues in the GGXR
CC motif define the oxyanion hole, and stabilize the oxyanion that forms
CC during the nucleophilic attack by the catalytic serine during substrate
CC cleavage.
CC -!- MISCELLANEOUS: Patatin have a dual role as a somatic storage protein
CC and as an enzyme involved in host resistance. This tuber protein
CC represents approximately 40% of the total protein in mature tubers.
CC -!- SIMILARITY: Belongs to the patatin family. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; M21879; AAA33828.1; -; mRNA.
DR EMBL; X13179; CAA31576.1; -; mRNA.
DR PIR; B26017; B26017.
DR PIR; S05593; S05593.
DR AlphaFoldDB; P15476; -.
DR SMR; P15476; -.
DR Allergome; 3489; Sola t 1.0101.
DR Allergome; 639; Sola t 1.
DR PRIDE; P15476; -.
DR InParanoid; P15476; -.
DR Proteomes; UP000011115; Unassembled WGS sequence.
DR ExpressionAtlas; P15476; baseline.
DR GO; GO:0005773; C:vacuole; IEA:UniProtKB-SubCell.
DR GO; GO:0047372; F:acylglycerol lipase activity; IBA:GO_Central.
DR GO; GO:0045735; F:nutrient reservoir activity; IEA:UniProtKB-KW.
DR GO; GO:0004620; F:phospholipase activity; IBA:GO_Central.
DR GO; GO:0006952; P:defense response; IEA:UniProtKB-KW.
DR GO; GO:0016042; P:lipid catabolic process; IEA:UniProtKB-KW.
DR InterPro; IPR016035; Acyl_Trfase/lysoPLipase.
DR InterPro; IPR002641; PNPLA_dom.
DR Pfam; PF01734; Patatin; 1.
DR SUPFAM; SSF52151; SSF52151; 1.
DR PROSITE; PS51635; PNPLA; 1.
PE 2: Evidence at transcript level;
KW Glycoprotein; Hydrolase; Lipid degradation; Lipid metabolism;
KW Plant defense; Reference proteome; Signal; Storage protein; Vacuole.
FT SIGNAL 1..23
FT CHAIN 24..386
FT /note="Patatin-B1"
FT /id="PRO_0000032257"
FT DOMAIN 32..229
FT /note="PNPLA"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01161"
FT MOTIF 36..41
FT /note="GXGXXG"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01161"
FT MOTIF 75..79
FT /note="GXSXG"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01161"
FT MOTIF 215..217
FT /note="DGA/G"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01161"
FT ACT_SITE 77
FT /note="Nucleophile"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01161"
FT ACT_SITE 215
FT /note="Proton acceptor"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01161"
FT CARBOHYD 115
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CONFLICT 290
FT /note="L -> M (in Ref. 1; AAA33828)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 386 AA; 42609 MW; F220B96112D8C665 CRC64;
MATTKSFLIL FFMILATTSS TCAKLEEMVT VLSIDGGGIK GIIPAIILEF LEGQLQEVDN
NKDARLADYF DVIGGTSTGG LLTAMITTPN ENNRPFAAAK DIVPFYFEHG PHIFNYSGSI
FGPRYDGKYL LQVLQEKLGE TRVHQALTEV AISSFDIKTN KPVIFTKSNL AKSPELDAKM
YDICYSIAAA PIYFPPHHFV THTSNGATYE FNLVDGGVAT VGDPALLSLS VATRLAQEDP
AFSSIKSLDY KQMLLLSLGT GTNSEFDKTY TAEEAAKWGP LRWMLAIQQL TNAASSYMTD
YYISTVFQAR HSQNNYLRVQ ENALTGTTTE MDDASEANME LLVQVGETLL KKPVSKDSPE
TYEEALKRFA KLLSNRKKLR ANKASY