PATL_ASPCL
ID PATL_ASPCL Reviewed; 809 AA.
AC A1CFL9;
DT 07-SEP-2016, integrated into UniProtKB/Swiss-Prot.
DT 23-JAN-2007, sequence version 1.
DT 03-AUG-2022, entry version 77.
DE RecName: Full=Patulin cluster transcription factor patL {ECO:0000303|PubMed:19383676};
DE AltName: Full=Patulin synthesis protein L {ECO:0000303|PubMed:19383676};
GN Name=patL {ECO:0000303|PubMed:19383676}; ORFNames=ACLA_093670;
OS Aspergillus clavatus (strain ATCC 1007 / CBS 513.65 / DSM 816 / NCTC 3887 /
OS NRRL 1 / QM 1276 / 107).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Eurotiomycetes;
OC Eurotiomycetidae; Eurotiales; Aspergillaceae; Aspergillus;
OC Aspergillus subgen. Fumigati.
OX NCBI_TaxID=344612;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 1007 / CBS 513.65 / DSM 816 / NCTC 3887 / NRRL 1;
RX PubMed=18404212; DOI=10.1371/journal.pgen.1000046;
RA Fedorova N.D., Khaldi N., Joardar V.S., Maiti R., Amedeo P., Anderson M.J.,
RA Crabtree J., Silva J.C., Badger J.H., Albarraq A., Angiuoli S., Bussey H.,
RA Bowyer P., Cotty P.J., Dyer P.S., Egan A., Galens K., Fraser-Liggett C.M.,
RA Haas B.J., Inman J.M., Kent R., Lemieux S., Malavazi I., Orvis J.,
RA Roemer T., Ronning C.M., Sundaram J.P., Sutton G., Turner G., Venter J.C.,
RA White O.R., Whitty B.R., Youngman P., Wolfe K.H., Goldman G.H.,
RA Wortman J.R., Jiang B., Denning D.W., Nierman W.C.;
RT "Genomic islands in the pathogenic filamentous fungus Aspergillus
RT fumigatus.";
RL PLoS Genet. 4:E1000046-E1000046(2008).
RN [2]
RP BIOTECHNOLOGY.
RX PubMed=15082620; DOI=10.1093/ije/dyh028;
RG Patulin Clinical Trials Committee, Medical Research Council;
RT "Clinical trial of patulin in the common cold. 1944.";
RL Int. J. Epidemiol. 33:243-246(2004).
RN [3]
RP FUNCTION.
RX PubMed=19383676; DOI=10.1099/mic.0.024836-0;
RA Artigot M.P., Loiseau N., Laffitte J., Mas-Reguieg L., Tadrist S.,
RA Oswald I.P., Puel O.;
RT "Molecular cloning and functional characterization of two CYP619 cytochrome
RT P450s involved in biosynthesis of patulin in Aspergillus clavatus.";
RL Microbiology 155:1738-1747(2009).
RN [4]
RP BIOTECHNOLOGY.
RX PubMed=22222931; DOI=10.1016/j.fct.2011.12.022;
RA de Melo F.T., de Oliveira I.M., Greggio S., Dacosta J.C., Guecheva T.N.,
RA Saffi J., Henriques J.A., Rosa R.M.;
RT "DNA damage in organs of mice treated acutely with patulin, a known
RT mycotoxin.";
RL Food Chem. Toxicol. 50:3548-3555(2012).
RN [5]
RP FUNCTION.
RX PubMed=24334092; DOI=10.1016/j.ijfoodmicro.2013.11.020;
RA Snini S.P., Tadrist S., Laffitte J., Jamin E.L., Oswald I.P., Puel O.;
RT "The gene PatG involved in the biosynthesis pathway of patulin, a food-
RT borne mycotoxin, encodes a 6-methylsalicylic acid decarboxylase.";
RL Int. J. Food Microbiol. 171:77-83(2014).
RN [6]
RP BIOTECHNOLOGY.
RX PubMed=26619846; DOI=10.1007/s13277-015-4474-z;
RA Boussabbeh M., Ben Salem I., Rjiba-Touati K., Bouyahya C., Neffati F.,
RA Najjar M.F., Bacha H., Abid-Essefi S.;
RT "The potential effect of patulin on mice bearing melanoma cells: an anti-
RT tumour or carcinogenic effect?";
RL Tumor Biol. 37:6285-6295(2016).
CC -!- FUNCTION: Transcription factor that regulates the expression of the
CC gene cluster that mediates the biosynthesis of patulin, an acetate-
CC derived tetraketide mycotoxin produced by several fungal species that
CC shows antimicrobial properties against several bacteria
CC (PubMed:19383676). {ECO:0000269|PubMed:19383676}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00227}.
CC -!- BIOTECHNOLOGY: Patulin was originally used as an antibiotic and
CC specifically trialed to be used against the common cold, but it is no
CC longer used for that purpose since it has been shown to induce
CC immunological, neurological and gastrointestinal effects
CC (PubMed:15082620). Genotoxic effects of patulin with dose-dependent
CC increase in DNA strand breaks in brain, liver and kidneys have been
CC detected in mice (PubMed:22222931). However, more recently, it has been
CC proposed that patulin might also have anti-tumor properties
CC (PubMed:26619846). {ECO:0000269|PubMed:15082620,
CC ECO:0000269|PubMed:22222931, ECO:0000269|PubMed:26619846}.
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DR EMBL; DS027052; EAW11668.1; -; Genomic_DNA.
DR RefSeq; XP_001273094.1; XM_001273093.1.
DR AlphaFoldDB; A1CFL9; -.
DR SMR; A1CFL9; -.
DR EnsemblFungi; EAW11668; EAW11668; ACLA_093670.
DR GeneID; 4704856; -.
DR KEGG; act:ACLA_093670; -.
DR VEuPathDB; FungiDB:ACLA_093670; -.
DR eggNOG; ENOG502SIGA; Eukaryota.
DR HOGENOM; CLU_016509_0_0_1; -.
DR OMA; CDWCRLN; -.
DR OrthoDB; 876077at2759; -.
DR Proteomes; UP000006701; Unassembled WGS sequence.
DR GO; GO:0005634; C:nucleus; ISS:GO_Central.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IEA:InterPro.
DR GO; GO:0008270; F:zinc ion binding; IEA:InterPro.
DR GO; GO:0140724; P:positive regulation of patulin biosynthetic process; ISS:GO_Central.
DR GO; GO:0006351; P:transcription, DNA-templated; IEA:InterPro.
DR CDD; cd00067; GAL4; 1.
DR Gene3D; 4.10.240.10; -; 1.
DR InterPro; IPR007219; Transcription_factor_dom_fun.
DR InterPro; IPR001138; Zn2-C6_fun-type_DNA-bd.
DR InterPro; IPR036864; Zn2-C6_fun-type_DNA-bd_sf.
DR Pfam; PF04082; Fungal_trans; 1.
DR Pfam; PF00172; Zn_clus; 1.
DR SMART; SM00906; Fungal_trans; 1.
DR SMART; SM00066; GAL4; 1.
DR SUPFAM; SSF57701; SSF57701; 1.
DR PROSITE; PS00463; ZN2_CY6_FUNGAL_1; 1.
DR PROSITE; PS50048; ZN2_CY6_FUNGAL_2; 1.
PE 1: Evidence at protein level;
KW Coiled coil; DNA-binding; Metal-binding; Nucleus; Reference proteome;
KW Transcription; Transcription regulation; Zinc.
FT CHAIN 1..809
FT /note="Patulin cluster transcription factor patL"
FT /id="PRO_0000437119"
FT DNA_BIND 37..63
FT /note="Zn(2)-C6 fungal-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00227"
FT REGION 1..28
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 118..146
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 199..225
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 683..710
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 71..107
FT /evidence="ECO:0000255"
FT COMPBIAS 1..19
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 118..145
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 809 AA; 89864 MW; C81ED8137B3D5FB3 CRC64;
MFSTFSANLE ATDASRDGSA TPVTAPRPKR SQVARACDWC RLNRIKCDDK QPCQNCQNRG
GHCSNTKPLE ATSLPAANRE IQRLRNKVKD LQEQIRTAKQ EAEIRAHAQA QIQASTGYAT
PPLSDTVNGS FSNSEQLSNT KEGWQGIPPA GNRAGVLYYG PMSSTYFVTR ITTYLAQAMN
QPLVDTKLEA CVARFDARTP AHRPSRSDAS PESQTEGTDD TEDLSRSQEE YFLNLLWQSF
HCVYPIISEP DFRRYYESLW SNTSPEGTRK PSPLVDVLLA VCMQYGSTFL VRGDENHEAE
DSSHSENANM PGHAFYQRAQ RLLQSELEHP SIMVLQSHVY SIIYLYNISL LNTAHINLGV
TLRIAHTLRL HLRPLDGTPP EVQELHRNIW WTLYRLDNQL SMTLGRPSLI QNSQVSCGLP
GDSRDHIRLS GTVLLSGHED ISWLSFHVQC IKLIFAVQGV QAAFQLKCSQ LLNGDEAKDI
YGDPRMTETL AEFLGREMTA VHEWVQNVPQ SLRNARKGAG EAFSTDRAAL NLDLYSPLWL
QRQRLLLELL YHHLLISTFR PFIRFPPTSS SLTPLADGHN ISCLNHAMAI TNILHQVLSE
TDVLRGWSPA FQYQWDAILC TLGFVLANPV CPPTPSARKS LQTAVRTLDL VGDHFPAAVN
AAQVVREVNC RADRLVDTFR QSLTRRQGQR TSPFSPTGLQ KMNPPASQLA FSSAAQTASI
PVLSDPLPPS MSASSLELVR ARTIHPVPSI EMMPSTSPTD LSPPLVTTAE SHSAGLPAVS
VDVIAGNEVH WMQASAMILD SWTDFTTNP
