ID   ASP1_ORYSI              Reviewed;         410 AA.
AC   A2ZC67; Q53NF8; Q6YNY7; Q6YNY8; Q71SA7;
DT   11-SEP-2007, integrated into UniProtKB/Swiss-Prot.
DT   11-SEP-2007, sequence version 2.
DT   03-AUG-2022, entry version 68.
DE   RecName: Full=Aspartic proteinase Asp1;
DE            Short=OSAP1;
DE            Short=OsAsp1;
DE            EC=3.4.23.-;
DE   AltName: Full=Nucellin-like protein;
DE   Flags: Precursor;
GN   Name=ASP1; ORFNames=OsI_034160;
OS   Oryza sativa subsp. indica (Rice).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC   Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX   NCBI_TaxID=39946;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], FUNCTION, TISSUE SPECIFICITY, AND
RP   DEVELOPMENTAL STAGE.
RC   STRAIN=cv. IR64;
RX   PubMed=15659452; DOI=10.1093/pcp/pci002;
RA   Bi X., Khush G.S., Bennett J.;
RT   "The rice nucellin gene ortholog OsAsp1 encodes an active aspartic protease
RT   without a plant-specific insert and is strongly expressed in early
RT   embryo.";
RL   Plant Cell Physiol. 46:87-98(2005).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. 93-11;
RX   PubMed=15685292; DOI=10.1371/journal.pbio.0030038;
RA   Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S.,
RA   Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L.,
RA   Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J.,
RA   Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X.,
RA   Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y.,
RA   Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L.,
RA   Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H.,
RA   Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z.,
RA   Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L.,
RA   Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F.,
RA   Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q.,
RA   Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J.,
RA   Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M.,
RA   McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.;
RT   "The genomes of Oryza sativa: a history of duplications.";
RL   PLoS Biol. 3:266-281(2005).
CC   -!- FUNCTION: Possesses protease activity in vitro.
CC       {ECO:0000269|PubMed:15659452}.
CC   -!- TISSUE SPECIFICITY: Expressed in pollen, nucellus, ovary wall, shoot
CC       and root meristem, coleoptiles of immature seeds, and somatic embryos.
CC       {ECO:0000269|PubMed:15659452}.
CC   -!- DEVELOPMENTAL STAGE: Abundantly expressed in zygotic embryos 1 and 2
CC       days after fertilization. {ECO:0000269|PubMed:15659452}.
CC   -!- SIMILARITY: Belongs to the peptidase A1 family. {ECO:0000305}.
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DR   EMBL; AY043216; AAK81698.1; -; Genomic_DNA.
DR   EMBL; AY043217; AAK81699.1; -; mRNA.
DR   EMBL; CM000136; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   AlphaFoldDB; A2ZC67; -.
DR   SMR; A2ZC67; -.
DR   MEROPS; A01.073; -.
DR   PRIDE; A2ZC67; -.
DR   Proteomes; UP000007015; Chromosome 11.
DR   GO; GO:0004190; F:aspartic-type endopeptidase activity; IEA:UniProtKB-KW.
DR   GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR   CDD; cd05475; nucellin_like; 1.
DR   Gene3D; 2.40.70.10; -; 2.
DR   InterPro; IPR001461; Aspartic_peptidase_A1.
DR   InterPro; IPR001969; Aspartic_peptidase_AS.
DR   InterPro; IPR033823; Nucellin.
DR   InterPro; IPR033121; PEPTIDASE_A1.
DR   InterPro; IPR021109; Peptidase_aspartic_dom_sf.
DR   InterPro; IPR032799; TAXi_C.
DR   InterPro; IPR032861; TAXi_N.
DR   PANTHER; PTHR13683; PTHR13683; 1.
DR   Pfam; PF14541; TAXi_C; 1.
DR   Pfam; PF14543; TAXi_N; 1.
DR   SUPFAM; SSF50630; SSF50630; 1.
DR   PROSITE; PS00141; ASP_PROTEASE; 2.
DR   PROSITE; PS51767; PEPTIDASE_A1; 1.
PE   2: Evidence at transcript level;
KW   Aspartyl protease; Hydrolase; Protease; Reference proteome; Repeat; Signal.
FT   SIGNAL          1..23
FT                   /evidence="ECO:0000255"
FT   PROPEP          24..46
FT                   /note="Removed in mature form"
FT                   /evidence="ECO:0000255"
FT                   /id="PRO_0000301660"
FT   CHAIN           47..410
FT                   /note="Aspartic proteinase Asp1"
FT                   /id="PRO_0000301661"
FT   DOMAIN          38..392
FT                   /note="Peptidase A1"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01103"
FT   ACT_SITE        56
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10094"
FT   ACT_SITE        257
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10094"
FT   CONFLICT        45
FT                   /note="G -> S (in Ref. 1; AAK81698/AAK81699)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        239
FT                   /note="Q -> H (in Ref. 1; AAK81698/AAK81699)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        244..245
FT                   /note="SK -> KQS (in Ref. 1; AAK81699)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   410 AA;  44961 MW;  AAC9775F3234FB9A CRC64;
     MTARLALLAS LLLLLQLVPP SSAVVLELHG NVYPIGHFFV TMNIGDPAKP YFLDIDTGST
     LTWLQCDYPC INCNKVPHGL YKPELKYAVK CTEQRCADLY ADLRKPMKCG PKNQCHYGIQ
     YVGGSSIGVL IVDSFSLPAS NGTNPTSIAF GCGYNQGKNN HNVPTPVNGI LGLGRGKVTL
     LSQLKSQGVI TKHVLGHCIS SKGKGFLFFG DAKVPTSGVT WSPMNREHKH YSPRQGTLQF
     NSNSKPISAA PMEVIFDSGA TYTYFALQPY HATLSVVKST LSKECKFLTE VKEKDRALTV
     CWKGKDKIRT IDEVKKCFRS LSLKFADGDK KATLEIPPEH YLIISQEGHV CLGILDGSKE
     HPSLAGTNLI GGITMLDQMV IYDSERSLLG WVNYQCDRIP RSASAITSRL