PAT_XENLA
ID PAT_XENLA Reviewed; 293 AA.
AC O73622; Q6GQH1;
DT 17-OCT-2006, integrated into UniProtKB/Swiss-Prot.
DT 08-NOV-2005, sequence version 2.
DT 03-AUG-2022, entry version 63.
DE RecName: Full=Protein Pat;
DE AltName: Full=Primordial germ cell-associated transcript protein;
DE AltName: Full=Xpat;
GN Name=pat {ECO:0000303|PubMed:9622619, ECO:0000312|EMBL:CAA05358.2};
OS Xenopus laevis (African clawed frog).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae; Xenopus; Xenopus.
OX NCBI_TaxID=8355;
RN [1] {ECO:0000305, ECO:0000312|EMBL:CAA05358.2}
RP NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, AND DEVELOPMENTAL STAGE.
RC TISSUE=Embryo {ECO:0000269|PubMed:9622619};
RX PubMed=9622619; DOI=10.1016/s0925-4773(98)00047-1;
RA Hudson C., Woodland H.R.;
RT "Xpat, a gene expressed specifically in germ plasm and primordial germ
RT cells of Xenopus laevis.";
RL Mech. Dev. 73:159-168(1998).
RN [2] {ECO:0000312|EMBL:AAH72773.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Embryo {ECO:0000312|EMBL:AAH72773.1};
RG NIH - Xenopus Gene Collection (XGC) project;
RL Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases.
RN [3] {ECO:0000305}
RP TISSUE SPECIFICITY.
RX PubMed=11784096; DOI=10.1006/dbio.2001.0488;
RA Kloc M., Dougherty M.T., Bilinski S., Chan A.P., Brey E., King M.L.,
RA Patrick C.W. Jr., Etkin L.D.;
RT "Three-dimensional ultrastructural analysis of RNA distribution within
RT germinal granules of Xenopus.";
RL Dev. Biol. 241:79-93(2002).
RN [4] {ECO:0000305}
RP FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, AND DEVELOPMENTAL
RP STAGE.
RX PubMed=16216237; DOI=10.1016/j.ydbio.2005.08.044;
RA Machado R.J., Moore W., Hames R., Houliston E., Chang P., King M.L.,
RA Woodland H.R.;
RT "Xenopus Xpat protein is a major component of germ plasm and may function
RT in its organisation and positioning.";
RL Dev. Biol. 287:289-300(2005).
RN [5] {ECO:0000305}
RP TISSUE SPECIFICITY.
RX PubMed=15744663; DOI=10.1387/ijdb.041906kw;
RA Wilk K., Bilinski S., Dougherty M.T., Kloc M.;
RT "Delivery of germinal granules and localized RNAs via the messenger
RT transport organizer pathway to the vegetal cortex of Xenopus oocytes occurs
RT through directional expansion of the mitochondrial cloud.";
RL Int. J. Dev. Biol. 49:17-21(2005).
RN [6] {ECO:0000305}
RP TISSUE SPECIFICITY.
RX PubMed=16287483; DOI=10.1111/j.1440-169x.2005.00826.x;
RA Kataoka K., Tazaki A., Kitayama A., Ueno N., Watanabe K., Mochii M.;
RT "Identification of asymmetrically localized transcripts along the animal-
RT vegetal axis of the Xenopus egg.";
RL Dev. Growth Differ. 47:511-521(2005).
RN [7]
RP INTERACTION WITH POC1B, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, AND
RP DEVELOPMENTAL STAGE.
RX PubMed=18068700; DOI=10.1016/j.yexcr.2007.10.019;
RA Hames R.S., Hames R., Prosser S.L., Euteneuer U., Lopes C.A., Moore W.,
RA Woodland H.R., Fry A.M.;
RT "Pix1 and Pix2 are novel WD40 microtubule-associated proteins that
RT colocalize with mitochondria in Xenopus germ plasm and centrosomes in human
RT cells.";
RL Exp. Cell Res. 314:574-589(2008).
CC -!- FUNCTION: Probably plays a role in germ plasm formation, positioning
CC and maintenance. {ECO:0000269|PubMed:16216237}.
CC -!- SUBUNIT: Interacts with poc1b. {ECO:0000269|PubMed:18068700}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:16216237,
CC ECO:0000269|PubMed:18068700}. Nucleus {ECO:0000269|PubMed:16216237}.
CC Note=Unlike the transcript, the protein is not exclusively localized to
CC germ plasm but is also present in the nuclei of early oocytes, of later
CC stage primordial germ cells (PGCs) and of some somatic cells, as well
CC as in their cytoplasm (PubMed:16216237).
CC -!- TISSUE SPECIFICITY: An mRNA and protein component of germ plasm and
CC primordial germ cells (PGCs) throughout oogenesis and early
CC development, being first localized to the granulo-fibrillar material
CC (GFM) of the mitochondrial cloud in stage I and II oocytes and to the
CC periphery of mature germinal granules both in oocytes and in embryos.
CC Shows some somatic expression including the ectodermal cells of tailbud
CC embryos. In adults, only expressed in ovaries.
CC {ECO:0000269|PubMed:11784096, ECO:0000269|PubMed:15744663,
CC ECO:0000269|PubMed:16216237, ECO:0000269|PubMed:16287483,
CC ECO:0000269|PubMed:18068700, ECO:0000269|PubMed:9622619}.
CC -!- DEVELOPMENTAL STAGE: Expressed both maternally and zygotically from at
CC least the pre-vitellogenic stage I of oogenesis through to the tadpole
CC stages. {ECO:0000269|PubMed:16216237, ECO:0000269|PubMed:18068700,
CC ECO:0000269|PubMed:9622619}.
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DR EMBL; AJ002384; CAA05358.2; -; mRNA.
DR EMBL; BC072773; AAH72773.1; -; mRNA.
DR RefSeq; NP_001080932.1; NM_001087463.1.
DR AlphaFoldDB; O73622; -.
DR SMR; O73622; -.
DR GeneID; 394273; -.
DR KEGG; xla:394273; -.
DR CTD; 394273; -.
DR Xenbase; XB-GENE-6254671; pgat.L.
DR Proteomes; UP000186698; Chromosome 5L.
DR Bgee; 394273; Expressed in ovary and 7 other tissues.
DR GO; GO:0005737; C:cytoplasm; IDA:UniProtKB.
DR GO; GO:0032019; C:mitochondrial cloud; IDA:UniProtKB.
DR GO; GO:0005634; C:nucleus; IDA:UniProtKB.
DR GO; GO:0043186; C:P granule; IDA:UniProtKB.
DR GO; GO:0045495; C:pole plasm; IDA:UniProtKB.
DR GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR GO; GO:0007315; P:pole plasm assembly; IMP:UniProtKB.
DR InterPro; IPR018379; BEN_domain.
DR PROSITE; PS51457; BEN; 1.
PE 1: Evidence at protein level;
KW Cytoplasm; Developmental protein; Differentiation; Nucleus; Oogenesis;
KW Reference proteome.
FT CHAIN 1..293
FT /note="Protein Pat"
FT /id="PRO_0000253962"
FT DOMAIN 187..287
FT /note="BEN"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00784"
SQ SEQUENCE 293 AA; 33427 MW; 4264C8B7CEC4021B CRC64;
MALKAEDSFD IYSQLIQSFC RYAENTTVSE SQSPAFNAQK EFQTCQDHAC CTHSEAHTHI
LMQQWQLLEE QWEYIDHLKT DVAALKQLLH GFMNSLSGTD SGMEGTNHFL PPHQNPTLLK
DEEIVASALN RPSVDINGFE ENITTGAQIH AAFTKSPKKM PATSTPRKSE VLWSCSPTLA
TDGYFMLPDI ILNPLDGKKL VSMLRSSNYE PHRFAELLFQ HHVPHSLFQL WANKVNFDGS
RGKLGLPRNL MIDILHQTSK RFVLGPKEKR KIKTRLNLLL RTRQDRAWWD VGL
