PAX3A_XENLA
ID PAX3A_XENLA Reviewed; 484 AA.
AC Q645N4; Q32NL3; Q5UCU1;
DT 03-MAR-2009, integrated into UniProtKB/Swiss-Prot.
DT 25-OCT-2004, sequence version 1.
DT 03-AUG-2022, entry version 110.
DE RecName: Full=Paired box protein Pax-3-A;
DE Short=xPax3-A;
DE AltName: Full=Paired-domain transcription factor Pax3-A;
GN Name=pax3-a;
GN Synonyms=pax3 {ECO:0000312|EMBL:AAI08574.1},
GN pax3A {ECO:0000303|PubMed:15843410};
OS Xenopus laevis (African clawed frog).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae; Xenopus; Xenopus.
OX NCBI_TaxID=8355;
RN [1] {ECO:0000305, ECO:0000312|EMBL:AAV31937.1}
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), FUNCTION, AND TISSUE SPECIFICITY.
RC TISSUE=Neurula {ECO:0000269|PubMed:15843410};
RX PubMed=15843410; DOI=10.1242/dev.01823;
RA Sato T., Sasai N., Sasai Y.;
RT "Neural crest determination by co-activation of Pax3 and Zic1 genes in
RT Xenopus ectoderm.";
RL Development 132:2355-2363(2005).
RN [2] {ECO:0000305, ECO:0000312|EMBL:AAU25940.1}
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
RA Wang E.P., Monsoro-Burq A.-H., Harland R.M.;
RL Submitted (AUG-2004) to the EMBL/GenBank/DDBJ databases.
RN [3] {ECO:0000305, ECO:0000312|EMBL:AAU25940.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC TISSUE=Neurula {ECO:0000312|EMBL:AAI08574.1};
RG NIH - Xenopus Gene Collection (XGC) project;
RL Submitted (NOV-2005) to the EMBL/GenBank/DDBJ databases.
RN [4] {ECO:0000305}
RP TISSUE SPECIFICITY, AND INDUCTION.
RX PubMed=9169853; DOI=10.1242/dev.124.10.2075;
RA Bang A.G., Papalopulu N., Kintner C., Goulding M.D.;
RT "Expression of Pax-3 is initiated in the early neural plate by
RT posteriorizing signals produced by the organizer and by posterior non-axial
RT mesoderm.";
RL Development 124:2075-2085(1997).
RN [5] {ECO:0000305}
RP TISSUE SPECIFICITY.
RX PubMed=10498689; DOI=10.1242/dev.126.20.4547;
RA Davidson L.A., Keller R.E.;
RT "Neural tube closure in Xenopus laevis involves medial migration, directed
RT protrusive activity, cell intercalation and convergent extension.";
RL Development 126:4547-4556(1999).
RN [6] {ECO:0000305}
RP TISSUE SPECIFICITY, AND INDUCTION.
RX PubMed=10433827; DOI=10.1006/dbio.1999.9319;
RA Bang A.G., Papalopulu N., Goulding M.D., Kintner C.;
RT "Expression of Pax-3 in the lateral neural plate is dependent on a Wnt-
RT mediated signal from posterior nonaxial mesoderm.";
RL Dev. Biol. 212:366-380(1999).
RN [7] {ECO:0000305}
RP TISSUE SPECIFICITY.
RX PubMed=15223346; DOI=10.1016/j.ydbio.2004.04.013;
RA Schlosser G., Ahrens K.;
RT "Molecular anatomy of placode development in Xenopus laevis.";
RL Dev. Biol. 271:439-466(2004).
RN [8] {ECO:0000305}
RP FUNCTION, AND TISSUE SPECIFICITY.
RX PubMed=15691759; DOI=10.1016/j.devcel.2004.12.017;
RA Monsoro-Burq A.-H., Wang E.P., Harland R.M.;
RT "Msx1 and Pax3 cooperate to mediate FGF8 and WNT signals during Xenopus
RT neural crest induction.";
RL Dev. Cell 8:167-178(2005).
RN [9] {ECO:0000305}
RP FUNCTION, AND TISSUE SPECIFICITY.
RX PubMed=17409353; DOI=10.1091/mbc.e06-11-1047;
RA Hong C.-S., Saint-Jeannet J.-P.;
RT "The activity of Pax3 and Zic1 regulates three distinct cell fates at the
RT neural plate border.";
RL Mol. Biol. Cell 18:2192-2202(2007).
CC -!- FUNCTION: Probable transcription factor. Promotes both hatching gland
CC and neural crest cell fates, two of the cell populations that arise
CC from the neural plate border. Acts downstream of msx1 to induce the
CC neural crest, cooperating with zic1 and mediating signals from both the
CC wnt and fgf8 signaling pathways. Induction of hatching gland cell fate
CC is independent of zic1. {ECO:0000269|PubMed:15691759,
CC ECO:0000269|PubMed:15843410, ECO:0000269|PubMed:17409353}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:P23760,
CC ECO:0000255|PROSITE-ProRule:PRU00108, ECO:0000255|PROSITE-
CC ProRule:PRU00381}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q645N4-1; Sequence=Displayed;
CC Name=2 {ECO:0000269|PubMed:15843410};
CC IsoId=Q645N4-2; Sequence=VSP_053008;
CC -!- TISSUE SPECIFICITY: Expressed in the dorsolateral ectoderm during early
CC and mid-gastrula stages. At late-gastrula and neurula stages, expressed
CC in the lateral neural plate, becoming progressively refined to the
CC neural folds during convergence and extension. Expression is also
CC restricted in the A/P axis, extending into the midbrain but excluded
CC from the forebrain. {ECO:0000269|PubMed:10433827,
CC ECO:0000269|PubMed:10498689, ECO:0000269|PubMed:15223346,
CC ECO:0000269|PubMed:15691759, ECO:0000269|PubMed:15843410,
CC ECO:0000269|PubMed:17409353, ECO:0000269|PubMed:9169853}.
CC -!- INDUCTION: By wnt-signaling from the posterior mesoderm.
CC {ECO:0000269|PubMed:10433827, ECO:0000269|PubMed:9169853}.
CC -!- SIMILARITY: Belongs to the paired homeobox family. {ECO:0000255}.
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DR EMBL; AY757358; AAV31937.1; -; mRNA.
DR EMBL; AY725269; AAU25940.1; -; mRNA.
DR EMBL; BC108573; AAI08574.1; -; mRNA.
DR RefSeq; NP_001088993.1; NM_001095524.1. [Q645N4-1]
DR RefSeq; XP_018120037.1; XM_018264548.1. [Q645N4-2]
DR AlphaFoldDB; Q645N4; -.
DR SMR; Q645N4; -.
DR DNASU; 496376; -.
DR GeneID; 496376; -.
DR KEGG; xla:496376; -.
DR CTD; 496376; -.
DR Xenbase; XB-GENE-482744; pax3.S.
DR OMA; HITCFHP; -.
DR Proteomes; UP000186698; Chromosome 5S.
DR Bgee; 496376; Expressed in neurula embryo and 5 other tissues.
DR GO; GO:0005634; C:nucleus; ISS:UniProtKB.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IEA:InterPro.
DR GO; GO:0008543; P:fibroblast growth factor receptor signaling pathway; IGI:UniProtKB.
DR GO; GO:0048785; P:hatching gland development; IMP:UniProtKB.
DR GO; GO:0007399; P:nervous system development; IEA:UniProtKB-KW.
DR GO; GO:0014034; P:neural crest cell fate commitment; IMP:UniProtKB.
DR GO; GO:0014029; P:neural crest formation; IMP:UniProtKB.
DR GO; GO:0045893; P:positive regulation of transcription, DNA-templated; ISS:UniProtKB.
DR GO; GO:0016055; P:Wnt signaling pathway; IGI:UniProtKB.
DR CDD; cd00086; homeodomain; 1.
DR CDD; cd00131; PAX; 1.
DR Gene3D; 1.10.10.10; -; 2.
DR InterPro; IPR009057; Homeobox-like_sf.
DR InterPro; IPR017970; Homeobox_CS.
DR InterPro; IPR001356; Homeobox_dom.
DR InterPro; IPR043182; PAIRED_DNA-bd_dom.
DR InterPro; IPR001523; Paired_dom.
DR InterPro; IPR022106; Pax7_C.
DR InterPro; IPR043565; PAX_fam.
DR InterPro; IPR036388; WH-like_DNA-bd_sf.
DR PANTHER; PTHR45636; PTHR45636; 1.
DR Pfam; PF00046; Homeodomain; 1.
DR Pfam; PF00292; PAX; 1.
DR Pfam; PF12360; Pax7; 1.
DR PRINTS; PR00027; PAIREDBOX.
DR SMART; SM00389; HOX; 1.
DR SMART; SM00351; PAX; 1.
DR SUPFAM; SSF46689; SSF46689; 2.
DR PROSITE; PS00027; HOMEOBOX_1; 1.
DR PROSITE; PS50071; HOMEOBOX_2; 1.
DR PROSITE; PS00034; PAIRED_1; 1.
DR PROSITE; PS51057; PAIRED_2; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Developmental protein; Differentiation; DNA-binding;
KW Homeobox; Neurogenesis; Nucleus; Paired box; Reference proteome;
KW Transcription; Transcription regulation; Wnt signaling pathway.
FT CHAIN 1..484
FT /note="Paired box protein Pax-3-A"
FT /id="PRO_0000366207"
FT DNA_BIND 34..161
FT /note="Paired"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00381"
FT DNA_BIND 220..279
FT /note="Homeobox"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00108"
FT REGION 37..93
FT /note="PAI subdomain"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00381"
FT REGION 113..161
FT /note="RED subdomain"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00381"
FT REGION 165..225
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 314..354
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 165..202
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 210..225
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 127..150
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:15843410, ECO:0000303|Ref.3"
FT /id="VSP_053008"
FT CONFLICT 223
FT /note="S -> G (in Ref. 3; AAI08574)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 484 AA; 53606 MW; F8823DFA2EEB3C71 CRC64;
MTSLAGAVPR MMRPCPGQNY PRTGFPLEVS TPLGQGRVNQ LGGVFINGRP LPNHIRHKIV
EMAHHGVRPC VISRQLRVSH GCVSKILCRY QETGSIRPGA IGGSKPKQVT TPEVEKKMEE
FKRDNPGMFS WEIRDKLLKD GVCDRNTVPS VSSISRILRS KFGKGDEEDM ELDRKEQEES
EKRAKHSIDG ILRERAPVSP ESEEGSDIDS EPDLPLKRKQ RRSRTTFTAE QLEELERAFE
RTHYPDIYTR EELAQRAKLT EARVQVWFSN RRARWRKQAG ANQLMAFNHL IPGAFPPAAM
PALPTYQLSE TSYQPTSIPQ AVSDPSNTVH RPQPLPPSSV HQSSLPSNPE SSSAYCLPSG
RHGFSSYTDS FVPPSGPSNP MNPAIGNGLS PQVMGLLTNH GGVPHQPQTD YALSPLTGGL
EPSTAVSASC SQRLEHMKSL DSLSTSQSYC PTYSTSGYSM EPVTGYQYPQ YGQSAFHYLK
PDIA
