PAX3H_CAEEL
ID PAX3H_CAEEL Reviewed; 308 AA.
AC G5ED66;
DT 29-SEP-2021, integrated into UniProtKB/Swiss-Prot.
DT 14-DEC-2011, sequence version 1.
DT 03-AUG-2022, entry version 84.
DE RecName: Full=Paired box protein 3 homolog {ECO:0000305};
GN Name=pax-3 {ECO:0000312|WormBase:F27E5.2};
GN ORFNames=F27E5.2 {ECO:0000312|WormBase:F27E5.2};
OS Caenorhabditis elegans.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC Caenorhabditis.
OX NCBI_TaxID=6239 {ECO:0000312|Proteomes:UP000001940};
RN [1] {ECO:0000312|Proteomes:UP000001940}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Bristol N2 {ECO:0000312|Proteomes:UP000001940};
RX PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG The C. elegans sequencing consortium;
RT "Genome sequence of the nematode C. elegans: a platform for investigating
RT biology.";
RL Science 282:2012-2018(1998).
RN [2] {ECO:0000305}
RP FUNCTION, SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE, DISRUPTION PHENOTYPE,
RP AND MUTAGENESIS OF PRO-29.
RX PubMed=26953187; DOI=10.1016/j.ydbio.2016.03.002;
RA Thompson K.W., Joshi P., Dymond J.S., Gorrepati L., Smith H.E.,
RA Krause M.W., Eisenmann D.M.;
RT "The Paired-box protein PAX-3 regulates the choice between lateral and
RT ventral epidermal cell fates in C. elegans.";
RL Dev. Biol. 412:191-207(2016).
CC -!- FUNCTION: Transcriptional activator (By similarity). Regulates the
CC lateral/ventral epidermal cell fate decision (PubMed:26953187).
CC {ECO:0000250|UniProtKB:P23760, ECO:0000269|PubMed:26953187}.
CC -!- INTERACTION:
CC G5ED66; O02482: unc-37; NbExp=5; IntAct=EBI-2416749, EBI-314716;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00108,
CC ECO:0000255|PROSITE-ProRule:PRU00381, ECO:0000269|PubMed:26953187}.
CC -!- DEVELOPMENTAL STAGE: Expressed throughout embryogenesis and early
CC larval development, predominantly in hypodermal cells, especially
CC ventral P cells and their descendants. {ECO:0000269|PubMed:26953187}.
CC -!- DISRUPTION PHENOTYPE: RNAi-mediated knockdown causes embryonic
CC lethality, vulval and gonad migration defects, and animals that survive
CC have mild to severe body morphology defects (PubMed:26953187). A marker
CC of the lateral hypodermal (seam cell) fate, scm, is expressed
CC ectopically in some P cell nuclei during the early larval L1 stage
CC (PubMed:26953187). {ECO:0000269|PubMed:26953187}.
CC -!- SIMILARITY: Belongs to the paired homeobox family. {ECO:0000305}.
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DR EMBL; BX284602; CAA88468.2; -; Genomic_DNA.
DR PIR; T21461; T21461.
DR RefSeq; NP_496189.2; NM_063788.2.
DR AlphaFoldDB; G5ED66; -.
DR SMR; G5ED66; -.
DR IntAct; G5ED66; 58.
DR STRING; 6239.F27E5.2; -.
DR EPD; G5ED66; -.
DR PaxDb; G5ED66; -.
DR EnsemblMetazoa; F27E5.2.1; F27E5.2.1; WBGene00003939.
DR GeneID; 185022; -.
DR KEGG; cel:CELE_F27E5.2; -.
DR CTD; 185022; -.
DR WormBase; F27E5.2; CE35862; WBGene00003939; pax-3.
DR eggNOG; KOG0849; Eukaryota.
DR HOGENOM; CLU_019281_1_3_1; -.
DR InParanoid; G5ED66; -.
DR OMA; RYYRTGI; -.
DR OrthoDB; 1014909at2759; -.
DR PhylomeDB; G5ED66; -.
DR Proteomes; UP000001940; Chromosome II.
DR Bgee; WBGene00003939; Expressed in embryo and 3 other tissues.
DR GO; GO:0005634; C:nucleus; IDA:UniProtKB.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0048856; P:anatomical structure development; IBA:GO_Central.
DR GO; GO:0009957; P:epidermal cell fate specification; IMP:UniProtKB.
DR GO; GO:0035262; P:gonad morphogenesis; IMP:UniProtKB.
DR GO; GO:0031581; P:hemidesmosome assembly; IGI:WormBase.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR CDD; cd00086; homeodomain; 1.
DR CDD; cd00131; PAX; 1.
DR Gene3D; 1.10.10.10; -; 2.
DR InterPro; IPR009057; Homeobox-like_sf.
DR InterPro; IPR001356; Homeobox_dom.
DR InterPro; IPR001523; Paired_dom.
DR InterPro; IPR043565; PAX_fam.
DR InterPro; IPR036388; WH-like_DNA-bd_sf.
DR PANTHER; PTHR45636; PTHR45636; 2.
DR Pfam; PF00046; Homeodomain; 1.
DR Pfam; PF00292; PAX; 1.
DR PRINTS; PR00027; PAIREDBOX.
DR SMART; SM00389; HOX; 1.
DR SMART; SM00351; PAX; 1.
DR SUPFAM; SSF46689; SSF46689; 2.
DR PROSITE; PS50071; HOMEOBOX_2; 1.
DR PROSITE; PS51057; PAIRED_2; 1.
PE 1: Evidence at protein level;
KW Developmental protein; DNA-binding; Homeobox; Nucleus; Paired box;
KW Reference proteome; Transcription; Transcription regulation.
FT CHAIN 1..308
FT /note="Paired box protein 3 homolog"
FT /id="PRO_0000453196"
FT DNA_BIND 13..140
FT /note="Paired"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00381"
FT DNA_BIND 187..246
FT /note="Homeobox"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00108"
FT REGION 16..72
FT /note="PAI subdomain"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00381"
FT REGION 92..140
FT /note="RED subdomain"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00381"
FT REGION 168..191
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 175..191
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MUTAGEN 29
FT /note="P->L: In ga96; causes defects in embryonic
FT development, larval body morphology and vulval development.
FT Adult hermaphrodites have gonad migration defects in which
FT one or both arms of the developing gonad fail to turn
FT correctly. Decrease in the number of ventral hypodermal
FT cells (Pn.p cells) and in the number of ventral cord
FT neurons (Pn.a cells). Ectopic expression of scm in P cells
FT in 27% of animals raised at the restrictive temperature, 25
FT degrees Celsius."
FT /evidence="ECO:0000269|PubMed:26953187"
SQ SEQUENCE 308 AA; 33854 MW; 521D9666C23886FE CRC64;
MTTDSITFNH ILGQGRVNQL GGVFINGRPL PIHVRHAIIS MAKKGIKPCH ISRQLKVSHG
AVSKILNRYA ETGSISPGQI GGSPRARLTV QAVEKEILIA CDENPQMSAA ELRDWLIHKD
ICTKGNAPTV PAIKRLIGNK GVGVPKKMER KRLSYSIDSI LGISIDECSK SSSDDEEGSS
PSNDASSRRN RTSFTAEQLD VLENAFRADT YPHANARESI SKETGLSEEK IMTWFSNRRA
RCRKNMPMYQ QYNVQGFGSS PPSYPTLLPS PMMFLPSYST SPQLNPLFFQ HILQSSPPSS
QSSPPSSS
