PAX4_RAT
ID PAX4_RAT Reviewed; 349 AA.
AC O88436; O88437; O88438; O88439;
DT 20-JUN-2001, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1998, sequence version 1.
DT 03-AUG-2022, entry version 156.
DE RecName: Full=Paired box protein Pax-4;
GN Name=Pax4;
OS Rattus norvegicus (Rat).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Rattus.
OX NCBI_TaxID=10116;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS A; B; C AND D).
RC TISSUE=Insulinoma;
RX PubMed=9675102; DOI=10.1006/bbrc.1998.8925;
RA Tokuyama Y., Yagui K., Sakurai K., Hashimoto N., Saito Y., Kanatsuka A.;
RT "Molecular cloning of rat Pax4: identification of four isoforms in rat
RT insulinoma cells.";
RL Biochem. Biophys. Res. Commun. 248:153-156(1998).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS A AND C).
RC TISSUE=Insulinoma;
RX PubMed=10601637; DOI=10.1016/s0014-5793(99)01584-7;
RA Campbell S.C., Cragg H., Elrick L.J., Macfarlane W.M., Shennan K.I.,
RA Docherty K.;
RT "Inhibitory effect of Pax4 on the human insulin and islet amyloid
RT polypeptide (IAPP) promoters.";
RL FEBS Lett. 463:53-57(1999).
CC -!- FUNCTION: Plays an important role in the differentiation and
CC development of pancreatic islet beta cells. Transcriptional repressor
CC that competes with PAX6 in binding to a common element in the glucagon,
CC insulin and somatostatin promoters (By similarity). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Nucleus.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=4;
CC Name=A;
CC IsoId=O88436-1; Sequence=Displayed;
CC Name=B;
CC IsoId=O88436-2; Sequence=VSP_002363;
CC Name=C;
CC IsoId=O88436-3; Sequence=VSP_002364, VSP_002365;
CC Name=D;
CC IsoId=O88436-4; Sequence=VSP_002363, VSP_002364, VSP_002365;
CC -!- TISSUE SPECIFICITY: Specifically expressed in pancreatic islets.
CC -!- SIMILARITY: Belongs to the paired homeobox family. {ECO:0000305}.
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DR EMBL; AF053100; AAC40195.1; -; mRNA.
DR EMBL; AF053101; AAC40196.1; -; mRNA.
DR EMBL; AF053102; AAC40197.1; -; mRNA.
DR EMBL; AF053103; AAC40198.1; -; mRNA.
DR EMBL; AF198155; AAF04858.1; -; mRNA.
DR EMBL; AF198156; AAF04859.1; -; mRNA.
DR PIR; JE0202; JE0202.
DR RefSeq; NP_113987.1; NM_031799.1. [O88436-1]
DR RefSeq; XP_006236294.1; XM_006236232.3. [O88436-1]
DR RefSeq; XP_006236295.1; XM_006236233.3. [O88436-1]
DR RefSeq; XP_017448403.1; XM_017592914.1. [O88436-1]
DR RefSeq; XP_017448405.1; XM_017592916.1. [O88436-2]
DR AlphaFoldDB; O88436; -.
DR SMR; O88436; -.
DR STRING; 10116.ENSRNOP00000010658; -.
DR CarbonylDB; O88436; -.
DR PaxDb; O88436; -.
DR Ensembl; ENSRNOT00000010658; ENSRNOP00000010658; ENSRNOG00000008020. [O88436-1]
DR Ensembl; ENSRNOT00000113994; ENSRNOP00000082503; ENSRNOG00000008020. [O88436-2]
DR GeneID; 83630; -.
DR KEGG; rno:83630; -.
DR UCSC; RGD:620433; rat. [O88436-1]
DR CTD; 5078; -.
DR RGD; 620433; Pax4.
DR eggNOG; KOG0849; Eukaryota.
DR GeneTree; ENSGT00940000161709; -.
DR HOGENOM; CLU_019281_1_2_1; -.
DR InParanoid; O88436; -.
DR OMA; RYYRTGI; -.
DR OrthoDB; 1152885at2759; -.
DR PhylomeDB; O88436; -.
DR PRO; PR:O88436; -.
DR Proteomes; UP000002494; Chromosome 4.
DR Bgee; ENSRNOG00000008020; Expressed in duodenum and 3 other tissues.
DR Genevisible; O88436; RN.
DR GO; GO:0005634; C:nucleus; IDA:RGD.
DR GO; GO:0003677; F:DNA binding; ISO:RGD.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0001227; F:DNA-binding transcription repressor activity, RNA polymerase II-specific; ISO:RGD.
DR GO; GO:0003690; F:double-stranded DNA binding; IDA:RGD.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; ISO:RGD.
DR GO; GO:1990837; F:sequence-specific double-stranded DNA binding; ISO:RGD.
DR GO; GO:0048856; P:anatomical structure development; IBA:GO_Central.
DR GO; GO:0007623; P:circadian rhythm; IEP:RGD.
DR GO; GO:0043066; P:negative regulation of apoptotic process; IMP:RGD.
DR GO; GO:0000122; P:negative regulation of transcription by RNA polymerase II; ISO:RGD.
DR GO; GO:0045892; P:negative regulation of transcription, DNA-templated; IDA:RGD.
DR GO; GO:0045597; P:positive regulation of cell differentiation; ISO:RGD.
DR GO; GO:0030858; P:positive regulation of epithelial cell differentiation; IEA:Ensembl.
DR GO; GO:0045595; P:regulation of cell differentiation; ISO:RGD.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR GO; GO:0051591; P:response to cAMP; IEP:RGD.
DR GO; GO:0009410; P:response to xenobiotic stimulus; IEP:RGD.
DR GO; GO:0060041; P:retina development in camera-type eye; IEP:RGD.
DR GO; GO:0003309; P:type B pancreatic cell differentiation; IEA:Ensembl.
DR CDD; cd00086; homeodomain; 1.
DR Gene3D; 1.10.10.10; -; 2.
DR InterPro; IPR009057; Homeobox-like_sf.
DR InterPro; IPR017970; Homeobox_CS.
DR InterPro; IPR001356; Homeobox_dom.
DR InterPro; IPR043182; PAIRED_DNA-bd_dom.
DR InterPro; IPR001523; Paired_dom.
DR InterPro; IPR033559; Pax-4.
DR InterPro; IPR043565; PAX_fam.
DR InterPro; IPR036388; WH-like_DNA-bd_sf.
DR PANTHER; PTHR45636; PTHR45636; 1.
DR PANTHER; PTHR45636:SF8; PTHR45636:SF8; 1.
DR Pfam; PF00046; Homeodomain; 1.
DR Pfam; PF00292; PAX; 1.
DR PRINTS; PR00027; PAIREDBOX.
DR SMART; SM00389; HOX; 1.
DR SMART; SM00351; PAX; 1.
DR SUPFAM; SSF46689; SSF46689; 2.
DR PROSITE; PS00027; HOMEOBOX_1; 1.
DR PROSITE; PS50071; HOMEOBOX_2; 1.
DR PROSITE; PS00034; PAIRED_1; 1.
DR PROSITE; PS51057; PAIRED_2; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Developmental protein; Differentiation; DNA-binding;
KW Homeobox; Nucleus; Paired box; Reference proteome; Repressor;
KW Transcription; Transcription regulation.
FT CHAIN 1..349
FT /note="Paired box protein Pax-4"
FT /id="PRO_0000050182"
FT DNA_BIND 5..131
FT /note="Paired"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00381"
FT DNA_BIND 170..229
FT /note="Homeobox"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00108"
FT REGION 8..64
FT /note="PAI subdomain"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00381"
FT REGION 83..131
FT /note="RED subdomain"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00381"
FT REGION 278..349
FT /note="Transcription repression"
FT VAR_SEQ 146..187
FT /note="Missing (in isoform B and isoform D)"
FT /evidence="ECO:0000303|PubMed:9675102"
FT /id="VSP_002363"
FT VAR_SEQ 217..223
FT /note="WFSNRRA -> SELWNTV (in isoform C and isoform D)"
FT /evidence="ECO:0000303|PubMed:10601637,
FT ECO:0000303|PubMed:9675102"
FT /id="VSP_002364"
FT VAR_SEQ 224..349
FT /note="Missing (in isoform C and isoform D)"
FT /evidence="ECO:0000303|PubMed:10601637,
FT ECO:0000303|PubMed:9675102"
FT /id="VSP_002365"
SQ SEQUENCE 349 AA; 38485 MW; A383009DBB0C0F8D CRC64;
MQQDGLSSVN QLGGLFVNGR PLPLDTRQQI VQLAIRGMRP CDISRSLKVS NGCVSKILGR
YYRTGVLEPK GIGGSKPRLA TPAVVARIAQ LKDEYPALFA WEIQRQLCAE GLCTQDKAPS
VSSINRVLRA LQEDQRLHWT QLRSPAVLAP ALPSPHSNCE APRGPHPGTS HRNRTIFSPG
QAEALEKEFQ RGQYPDSVVR GKLAAATSLP EDTVRVWFSN RRAKWRRQEK LKWETQMPGA
SQDLMVPKDS PGIISAQQSP GSVPSAALPV LEQLNPSFCQ LCWGAVPDRC SSDTTSQACL
QPYWECHSLL PVASSSYMEF AWPCLTTHPV HHLIGGPGQA PSTYYLHWP
