PAX5_MOUSE
ID PAX5_MOUSE Reviewed; 391 AA.
AC Q02650;
DT 01-JUL-1993, integrated into UniProtKB/Swiss-Prot.
DT 01-JUL-1993, sequence version 1.
DT 03-AUG-2022, entry version 170.
DE RecName: Full=Paired box protein Pax-5;
DE AltName: Full=B-cell-specific transcription factor;
DE Short=BSAP;
GN Name=Pax5; Synonyms=Pax-5;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE, AND TISSUE
RP SPECIFICITY.
RX PubMed=1516825; DOI=10.1101/gad.6.9.1589;
RA Adams B., Doerfler P., Aguzzi A., Kozmik Z., Urbanek P., Maurer-Fogy I.,
RA Busslinger M.;
RT "Pax-5 encodes the transcription factor BSAP and is expressed in B
RT lymphocytes, the developing CNS, and adult testis.";
RL Genes Dev. 6:1589-1607(1992).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 17-136.
RX PubMed=1685142; DOI=10.1016/0888-7543(91)90151-4;
RA Walther C., Guenet J.-L., Simon D., Deutsch U., Jostes B., Goulding M.D.,
RA Plachov D., Balling R., Gruss P.;
RT "Pax: a murine multigene family of paired box-containing genes.";
RL Genomics 11:424-434(1991).
RN [3]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=9042861; DOI=10.1101/gad.11.4.476;
RA Nutt S.L., Urbanek P., Rolink A., Busslinger M.;
RT "Essential functions of Pax5 (BSAP) in pro-B cell development: difference
RT between fetal and adult B lymphopoiesis and reduced V-to-DJ recombination
RT at the IgH locus.";
RL Genes Dev. 11:476-491(1997).
RN [4]
RP INTERACTION WITH DAXX.
RX PubMed=11799127; DOI=10.1074/jbc.m111763200;
RA Emelyanov A.V., Kovac C.R., Sepulveda M.A., Birshtein B.K.;
RT "The interaction of Pax5 (BSAP) with Daxx can result in transcriptional
RT activation in B cells.";
RL J. Biol. Chem. 277:11156-11164(2002).
RN [5]
RP FUNCTION.
RX PubMed=16546096; DOI=10.1016/j.immuni.2006.01.012;
RA Delogu A., Schebesta A., Sun Q., Aschenbrenner K., Perlot T.,
RA Busslinger M.;
RT "Gene repression by Pax5 in B cells is essential for blood cell homeostasis
RT and is reversed in plasma cells.";
RL Immunity 24:269-281(2006).
CC -!- FUNCTION: Transcription factor that plays an essential role in
CC commitment of lymphoid progenitors to the B-lymphocyte lineage
CC (PubMed:9042861). Fulfills a dual role by repressing B-lineage
CC inappropriate genes and simultaneously activating B-lineage-specific
CC genes (PubMed:16546096). In turn, regulates cell adhesion and
CC migration, induces V(H)-to-D(H)J(H) recombination, facilitates pre-B-
CC cell receptor signaling and promotes development to the mature B-cell
CC stage (PubMed:9042861, PubMed:16546096). Repression of the cohesin-
CC release factor WAPL causes global changes of the chromosomal
CC architecture in pro-B cells to facilitate the generation of a diverse
CC antibody repertoire (By similarity). {ECO:0000250|UniProtKB:Q02548,
CC ECO:0000269|PubMed:16546096, ECO:0000269|PubMed:9042861}.
CC -!- SUBUNIT: Interacts with ETS1; this interaction alters PAX5 DNA-binding
CC properties. Binds DNA as a monomer. Interacts with TBP; this
CC interaction allows PAX5 to interact with the basal transcription
CC machinery. Interacts with RB1. Interacts with TLE4 (By similarity).
CC Interacts with DAXX (PubMed:11799127). {ECO:0000250|UniProtKB:Q02548,
CC ECO:0000269|PubMed:11799127}.
CC -!- INTERACTION:
CC Q02650; Q6NZQ4: Paxip1; NbExp=3; IntAct=EBI-296260, EBI-1395317;
CC Q02650; Q9UER7: DAXX; Xeno; NbExp=4; IntAct=EBI-296260, EBI-77321;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:Q02548}.
CC -!- TISSUE SPECIFICITY: Expressed in all B-lymphoid organs, in the
CC embryonic midbrain and in adult testis. {ECO:0000269|PubMed:1516825}.
CC -!- PTM: O-glycosylated. {ECO:0000305}.
CC -!- PTM: Phosphorylated by SYK. This phosphorylation plays an important
CC role in the abolition of BLIMP1 repression by PAX5 in order to trigger
CC plasma cell differentiation. {ECO:0000250|UniProtKB:Q02548}.
CC -!- DISRUPTION PHENOTYPE: Mutant mice fail to produce small pre-B, B, and
CC plasma cells and therefore lack any immunoglobulin in their serum owing
CC to a complete arrest of B-cell development at an early stage.
CC {ECO:0000269|PubMed:9042861}.
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DR EMBL; M97013; AAA37325.1; -; mRNA.
DR CCDS; CCDS18125.1; -.
DR PIR; B41061; B41061.
DR RefSeq; NP_032808.1; NM_008782.2.
DR AlphaFoldDB; Q02650; -.
DR SMR; Q02650; -.
DR BioGRID; 202032; 4.
DR IntAct; Q02650; 9.
DR MINT; Q02650; -.
DR STRING; 10090.ENSMUSP00000014174; -.
DR iPTMnet; Q02650; -.
DR PhosphoSitePlus; Q02650; -.
DR MaxQB; Q02650; -.
DR PaxDb; Q02650; -.
DR PRIDE; Q02650; -.
DR ProteomicsDB; 294020; -.
DR Antibodypedia; 3668; 966 antibodies from 49 providers.
DR DNASU; 18507; -.
DR Ensembl; ENSMUST00000014174; ENSMUSP00000014174; ENSMUSG00000014030.
DR GeneID; 18507; -.
DR KEGG; mmu:18507; -.
DR UCSC; uc008srx.1; mouse.
DR CTD; 5079; -.
DR MGI; MGI:97489; Pax5.
DR VEuPathDB; HostDB:ENSMUSG00000014030; -.
DR eggNOG; KOG3862; Eukaryota.
DR GeneTree; ENSGT00940000159636; -.
DR InParanoid; Q02650; -.
DR OMA; IXIQESP; -.
DR OrthoDB; 592933at2759; -.
DR PhylomeDB; Q02650; -.
DR TreeFam; TF315397; -.
DR Reactome; R-MMU-8939245; RUNX1 regulates transcription of genes involved in BCR signaling.
DR BioGRID-ORCS; 18507; 1 hit in 75 CRISPR screens.
DR PRO; PR:Q02650; -.
DR Proteomes; UP000000589; Chromosome 4.
DR RNAct; Q02650; protein.
DR Bgee; ENSMUSG00000014030; Expressed in peripheral lymph node and 97 other tissues.
DR ExpressionAtlas; Q02650; baseline and differential.
DR Genevisible; Q02650; MM.
DR GO; GO:0005829; C:cytosol; ISO:MGI.
DR GO; GO:0001650; C:fibrillar center; ISO:MGI.
DR GO; GO:0043231; C:intracellular membrane-bounded organelle; ISO:MGI.
DR GO; GO:0005654; C:nucleoplasm; ISO:MGI.
DR GO; GO:0005634; C:nucleus; IDA:MGI.
DR GO; GO:0005667; C:transcription regulator complex; TAS:MGI.
DR GO; GO:0003677; F:DNA binding; IDA:MGI.
DR GO; GO:0001228; F:DNA-binding transcription activator activity, RNA polymerase II-specific; IDA:NTNU_SB.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; IDA:MGI.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IDA:NTNU_SB.
DR GO; GO:0030534; P:adult behavior; IMP:MGI.
DR GO; GO:0007568; P:aging; IMP:MGI.
DR GO; GO:0048856; P:anatomical structure development; IBA:GO_Central.
DR GO; GO:0009887; P:animal organ morphogenesis; TAS:MGI.
DR GO; GO:0021987; P:cerebral cortex development; IMP:MGI.
DR GO; GO:0071542; P:dopaminergic neuron differentiation; TAS:ParkinsonsUK-UCL.
DR GO; GO:0048701; P:embryonic cranial skeleton morphogenesis; IMP:MGI.
DR GO; GO:0021670; P:lateral ventricle development; IMP:MGI.
DR GO; GO:0051573; P:negative regulation of histone H3-K9 methylation; IMP:MGI.
DR GO; GO:0000122; P:negative regulation of transcription by RNA polymerase II; IDA:MGI.
DR GO; GO:0045892; P:negative regulation of transcription, DNA-templated; TAS:UniProtKB.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IDA:NTNU_SB.
DR GO; GO:0045893; P:positive regulation of transcription, DNA-templated; TAS:UniProtKB.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; IDA:MGI.
DR GO; GO:0035914; P:skeletal muscle cell differentiation; IMP:MGI.
DR GO; GO:0007283; P:spermatogenesis; IEA:UniProtKB-KW.
DR CDD; cd00131; PAX; 1.
DR Gene3D; 1.10.10.10; -; 2.
DR InterPro; IPR009057; Homeobox-like_sf.
DR InterPro; IPR043182; PAIRED_DNA-bd_dom.
DR InterPro; IPR001523; Paired_dom.
DR InterPro; IPR022130; Pax2_C.
DR InterPro; IPR043565; PAX_fam.
DR InterPro; IPR036388; WH-like_DNA-bd_sf.
DR PANTHER; PTHR45636; PTHR45636; 1.
DR Pfam; PF00292; PAX; 1.
DR Pfam; PF12403; Pax2_C; 1.
DR PRINTS; PR00027; PAIREDBOX.
DR SMART; SM00351; PAX; 1.
DR SUPFAM; SSF46689; SSF46689; 1.
DR PROSITE; PS00034; PAIRED_1; 1.
DR PROSITE; PS51057; PAIRED_2; 1.
PE 1: Evidence at protein level;
KW Developmental protein; Differentiation; Direct protein sequencing;
KW DNA-binding; Glycoprotein; Neurogenesis; Nucleus; Paired box;
KW Reference proteome; Spermatogenesis; Transcription;
KW Transcription regulation.
FT CHAIN 1..391
FT /note="Paired box protein Pax-5"
FT /id="PRO_0000050184"
FT DNA_BIND 16..142
FT /note="Paired"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00381"
FT REGION 19..75
FT /note="PAI subdomain"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00381"
FT REGION 94..142
FT /note="RED subdomain"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00381"
FT REGION 182..218
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 391 AA; 42231 MW; D703E94808DE8EC8 CRC64;
MDLEKNYPTP RTIRTGHGGV NQLGGVFVNG RPLPDVVRQR IVELAHQGVR PCDISRQLRV
SHGCVSKILG RYYETGSIKP GVIGGSKPKV ATPKVVEKIA EYKRQNPTMF AWEIRDRLLA
ERVCDNDTVP SVSSINRIIR TKVQQPPNQP VPASSHSIVS TGSVTQVSSV STDSAGSSYS
ISGILGITSP SADTNKRKRD EGIQESPVPN GHSLPGRDFL RKQMRGDLFT QQQLEVLDRV
FERQHYSDIF TTTEPIKPEQ TTEYSAMASL AGGLDDMKAN LTSPTPADIG SSVPGPQSYP
IVTGRDLAST TLPGYPPHVP PAGQGSYSAP TLTGMVPGSE FSGSPYSHPQ YSSYNDSWRF
PNPGLLGSPY YYSPAARGAA PPAAATAYDR H
