PAX8_MOUSE
ID PAX8_MOUSE Reviewed; 457 AA.
AC Q00288; P97342; Q6GU20;
DT 01-DEC-1992, integrated into UniProtKB/Swiss-Prot.
DT 27-JUL-2011, sequence version 3.
DT 03-AUG-2022, entry version 164.
DE RecName: Full=Paired box protein Pax-8;
GN Name=Pax8; Synonyms=Pax-8;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=1723950; DOI=10.1242/dev.110.2.643;
RA Plachov D., Chowdhury K., Walther C., Simon D., Guenet J.-L., Gruss P.;
RT "Pax8, a murine paired box gene expressed in the developing excretory
RT system and thyroid gland.";
RL Development 110:643-651(1990).
RN [2]
RP SEQUENCE REVISION TO 158; 263 AND 340.
RX PubMed=1516825; DOI=10.1101/gad.6.9.1589;
RA Adams B., Doerfler P., Aguzzi A., Kozmik Z., Urbanek P., Maurer-Fogy I.,
RA Busslinger M.;
RT "Pax-5 encodes the transcription factor BSAP and is expressed in B
RT lymphocytes, the developing CNS, and adult testis.";
RL Genes Dev. 6:1589-1607(1992).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=C57BL/6J; TISSUE=Kidney;
RX PubMed=9205117; DOI=10.1006/geno.1997.4735;
RA Okladnova O., Poleev A., Fantes J., Lee M., Plachov D., Horst J.;
RT "The genomic organization of the murine Pax 8 gene and characterization of
RT its basal promoter.";
RL Genomics 42:452-461(1997).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.;
RL Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=FVB/N; TISSUE=Kidney;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [7]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-304, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Kidney;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: Thought to encode a transcription factor. It may have a role
CC in kidney cell differentiation. May play a regulatory role in mammalian
CC development.
CC -!- SUBUNIT: Interacts with WWTR1. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Nucleus.
CC -!- TISSUE SPECIFICITY: Expressed in the developing excretory system and
CC the thyroid gland.
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DR EMBL; X57487; CAA40725.1; -; mRNA.
DR EMBL; X99592; CAA67904.1; -; Genomic_DNA.
DR EMBL; X99593; CAA67904.1; JOINED; Genomic_DNA.
DR EMBL; X99594; CAA67904.1; JOINED; Genomic_DNA.
DR EMBL; X99595; CAA67904.1; JOINED; Genomic_DNA.
DR EMBL; X99596; CAA67904.1; JOINED; Genomic_DNA.
DR EMBL; X99597; CAA67904.1; JOINED; Genomic_DNA.
DR EMBL; X99598; CAA67904.1; JOINED; Genomic_DNA.
DR EMBL; AL732528; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CH466542; EDL08172.1; -; Genomic_DNA.
DR EMBL; BC020526; AAH20526.1; -; mRNA.
DR CCDS; CCDS15738.1; -.
DR PIR; A56925; A56925.
DR RefSeq; NP_035170.1; NM_011040.4.
DR AlphaFoldDB; Q00288; -.
DR BMRB; Q00288; -.
DR SMR; Q00288; -.
DR BioGRID; 202035; 4.
DR IntAct; Q00288; 3.
DR STRING; 10090.ENSMUSP00000028355; -.
DR iPTMnet; Q00288; -.
DR PhosphoSitePlus; Q00288; -.
DR MaxQB; Q00288; -.
DR PaxDb; Q00288; -.
DR PRIDE; Q00288; -.
DR ProteomicsDB; 294390; -.
DR Antibodypedia; 9626; 907 antibodies from 48 providers.
DR DNASU; 18510; -.
DR Ensembl; ENSMUST00000028355; ENSMUSP00000028355; ENSMUSG00000026976.
DR GeneID; 18510; -.
DR KEGG; mmu:18510; -.
DR UCSC; uc008ipb.2; mouse.
DR CTD; 7849; -.
DR MGI; MGI:97492; Pax8.
DR VEuPathDB; HostDB:ENSMUSG00000026976; -.
DR eggNOG; KOG3862; Eukaryota.
DR GeneTree; ENSGT00940000161868; -.
DR InParanoid; Q00288; -.
DR OMA; QLRTEAY; -.
DR OrthoDB; 592933at2759; -.
DR PhylomeDB; Q00288; -.
DR TreeFam; TF315397; -.
DR BioGRID-ORCS; 18510; 8 hits in 74 CRISPR screens.
DR ChiTaRS; Pax8; mouse.
DR PRO; PR:Q00288; -.
DR Proteomes; UP000000589; Chromosome 2.
DR RNAct; Q00288; protein.
DR Bgee; ENSMUSG00000026976; Expressed in thyroid gland and 95 other tissues.
DR ExpressionAtlas; Q00288; baseline and differential.
DR Genevisible; Q00288; MM.
DR GO; GO:0005654; C:nucleoplasm; ISS:UniProtKB.
DR GO; GO:0005634; C:nucleus; IDA:MGI.
DR GO; GO:0005667; C:transcription regulator complex; TAS:MGI.
DR GO; GO:0003677; F:DNA binding; ISS:UniProtKB.
DR GO; GO:0001228; F:DNA-binding transcription activator activity, RNA polymerase II-specific; ISO:MGI.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; ISS:UniProtKB.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; ISS:UniProtKB.
DR GO; GO:0043565; F:sequence-specific DNA binding; ISO:MGI.
DR GO; GO:1990837; F:sequence-specific double-stranded DNA binding; ISO:MGI.
DR GO; GO:0000976; F:transcription cis-regulatory region binding; IDA:UniProtKB.
DR GO; GO:0048856; P:anatomical structure development; IBA:GO_Central.
DR GO; GO:0009887; P:animal organ morphogenesis; TAS:MGI.
DR GO; GO:0001658; P:branching involved in ureteric bud morphogenesis; IEA:Ensembl.
DR GO; GO:0071371; P:cellular response to gonadotropin stimulus; ISO:MGI.
DR GO; GO:0007417; P:central nervous system development; IEP:UniProtKB.
DR GO; GO:0030855; P:epithelial cell differentiation; IMP:MGI.
DR GO; GO:0042472; P:inner ear morphogenesis; IGI:UniProtKB.
DR GO; GO:0072073; P:kidney epithelium development; IEP:UniProtKB.
DR GO; GO:0003337; P:mesenchymal to epithelial transition involved in metanephros morphogenesis; IEA:Ensembl.
DR GO; GO:0072164; P:mesonephric tubule development; IEP:UniProtKB.
DR GO; GO:0001823; P:mesonephros development; IMP:UniProtKB.
DR GO; GO:0072278; P:metanephric comma-shaped body morphogenesis; IEA:Ensembl.
DR GO; GO:0072221; P:metanephric distal convoluted tubule development; IMP:UniProtKB.
DR GO; GO:0072289; P:metanephric nephron tubule formation; IMP:UniProtKB.
DR GO; GO:0072284; P:metanephric S-shaped body morphogenesis; IEA:Ensembl.
DR GO; GO:0001656; P:metanephros development; IGI:MGI.
DR GO; GO:1900215; P:negative regulation of apoptotic process involved in metanephric collecting duct development; IMP:UniProtKB.
DR GO; GO:1900218; P:negative regulation of apoptotic process involved in metanephric nephron tubule development; IMP:UniProtKB.
DR GO; GO:0010667; P:negative regulation of cardiac muscle cell apoptotic process; IMP:BHF-UCL.
DR GO; GO:0072305; P:negative regulation of mesenchymal cell apoptotic process involved in metanephric nephron morphogenesis; IMP:UniProtKB.
DR GO; GO:1900212; P:negative regulation of mesenchymal cell apoptotic process involved in metanephros development; IMP:UniProtKB.
DR GO; GO:0071599; P:otic vesicle development; IEA:Ensembl.
DR GO; GO:0090190; P:positive regulation of branching involved in ureteric bud morphogenesis; IMP:UniProtKB.
DR GO; GO:0072108; P:positive regulation of mesenchymal to epithelial transition involved in metanephros morphogenesis; IMP:UniProtKB.
DR GO; GO:2000594; P:positive regulation of metanephric DCT cell differentiation; IMP:UniProtKB.
DR GO; GO:2000611; P:positive regulation of thyroid hormone generation; IMP:UniProtKB.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; ISO:MGI.
DR GO; GO:0045893; P:positive regulation of transcription, DNA-templated; IDA:UniProtKB.
DR GO; GO:0039003; P:pronephric field specification; IEP:UniProtKB.
DR GO; GO:0048793; P:pronephros development; IMP:UniProtKB.
DR GO; GO:0042981; P:regulation of apoptotic process; IMP:UniProtKB.
DR GO; GO:0072307; P:regulation of metanephric nephron tubule epithelial cell differentiation; IMP:UniProtKB.
DR GO; GO:2000612; P:regulation of thyroid-stimulating hormone secretion; ISS:UniProtKB.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; ISO:MGI.
DR GO; GO:0072050; P:S-shaped body morphogenesis; IEP:UniProtKB.
DR GO; GO:0006790; P:sulfur compound metabolic process; IEA:Ensembl.
DR GO; GO:0030878; P:thyroid gland development; IMP:MGI.
DR GO; GO:0006351; P:transcription, DNA-templated; ISO:MGI.
DR GO; GO:0001655; P:urogenital system development; IGI:UniProtKB.
DR GO; GO:0003281; P:ventricular septum development; IMP:BHF-UCL.
DR CDD; cd00131; PAX; 1.
DR Gene3D; 1.10.10.10; -; 2.
DR InterPro; IPR009057; Homeobox-like_sf.
DR InterPro; IPR043182; PAIRED_DNA-bd_dom.
DR InterPro; IPR001523; Paired_dom.
DR InterPro; IPR022130; Pax2_C.
DR InterPro; IPR043565; PAX_fam.
DR InterPro; IPR036388; WH-like_DNA-bd_sf.
DR PANTHER; PTHR45636; PTHR45636; 1.
DR Pfam; PF00292; PAX; 1.
DR Pfam; PF12403; Pax2_C; 1.
DR PRINTS; PR00027; PAIREDBOX.
DR SMART; SM00351; PAX; 1.
DR SUPFAM; SSF46689; SSF46689; 1.
DR PROSITE; PS00034; PAIRED_1; 1.
DR PROSITE; PS51057; PAIRED_2; 1.
PE 1: Evidence at protein level;
KW Developmental protein; Differentiation; DNA-binding; Nucleus; Paired box;
KW Phosphoprotein; Reference proteome; Transcription;
KW Transcription regulation.
FT CHAIN 1..457
FT /note="Paired box protein Pax-8"
FT /id="PRO_0000050198"
FT DNA_BIND 9..135
FT /note="Paired"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00381"
FT REGION 12..68
FT /note="PAI subdomain"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00381"
FT REGION 87..135
FT /note="RED subdomain"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00381"
FT REGION 159..224
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 159..188
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 208..224
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 304
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT CONFLICT 340
FT /note="G -> D (in Ref. 2; no nucleotide entry)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 457 AA; 48793 MW; AAACD3EE54AA4488 CRC64;
MPHNSIRSGH GGLNQLGGAF VNGRPLPEVV RQRIVDLAHQ GVRPCDISRQ LRVSHGCVSK
ILGRYYETGS IRPGVIGGSK PKVATPKVVE KIGDYKRQNP TMFAWEIRDR LLAEGVCDND
TVPSVSSINR IIRTKVQQPF NLPMDSCVAT KSLSPGHTLI PSSAVTPPES PQSDSLGSTY
SINGLLGIAQ PGNDNKRKMD DSDQDSCRLS IDSQSSSSGP RKHLRTDTFS QHHLEALECP
FERQHYPEAY ASPSHTKGEQ GLYPLPLLNS ALDDGKATLT SSNTPLGRNL STHQTYPVVA
DPHSPFAIKQ ETPELSSSSS TPSSLSSSAF LDLQQVGSGG PAGASVPPFN AFPHAASVYG
QFTGQALLSG REMVGPTLPG YPPHIPTSGQ GSYASSAIAG MVAGSEYSGN AYSHTPYSSY
SEAWRFPNSS LLSSPYYYSS TSRPSAPPTS ATAFDHL
