PA_THOGV
ID PA_THOGV Reviewed; 622 AA.
AC P27194; O41354;
DT 01-AUG-1992, integrated into UniProtKB/Swiss-Prot.
DT 30-MAY-2000, sequence version 2.
DT 29-SEP-2021, entry version 82.
DE RecName: Full=Polymerase acidic protein;
DE Short=PA;
DE AltName: Full=RNA-directed RNA polymerase subunit P3;
GN OrderedLocusNames=Segment 3;
OS Thogoto virus (isolate SiAr 126) (Tho).
OC Viruses; Riboviria; Orthornavirae; Negarnaviricota; Polyploviricotina;
OC Insthoviricetes; Articulavirales; Orthomyxoviridae; Thogotovirus.
OX NCBI_TaxID=126796;
OH NCBI_TaxID=34610; Amblyomma variegatum (Tropical bont tick).
OH NCBI_TaxID=10144; Cavia cutleri (Guinea pig).
OH NCBI_TaxID=210652; Mungos mungo (Banded mongoose).
OH NCBI_TaxID=34631; Rhipicephalus appendiculatus (Brown ear tick).
OH NCBI_TaxID=6941; Rhipicephalus microplus (Cattle tick) (Boophilus microplus).
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=2794982; DOI=10.1099/0022-1317-70-10-2811;
RA Staunton D., Nuttall P.A., Bishop D.H.L.;
RT "Sequence analyses of Thogoto viral RNA segment 3: evidence for a distant
RT relationship between an arbovirus and members of the Orthomyxoviridae.";
RL J. Gen. Virol. 70:2811-2817(1989).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=9282786; DOI=10.1016/s0168-1702(97)00072-5;
RA Leahy M.B., Dessens J.T., Weber F., Kochs G., Nuttall P.A.;
RT "The fourth genus in the Orthomyxoviridae: sequence analyses of two Thogoto
RT virus polymerase proteins and comparison with influenza viruses.";
RL Virus Res. 50:215-224(1997).
CC -!- FUNCTION: subunit of the RNA-dependent RNA polymerase which is
CC responsible for replication and transcription of virus RNA segments.
CC The transcription of viral mRNAs occurs by a unique mechanism called
CC cap-snatching. 5' methylated caps of cellular mRNAs are cleaved after
CC 10-13 nucleotides by PA. In turn, these short capped RNAs are used as
CC primers by PB1 for transcription of viral mRNAs. During virus
CC replication, PB1 initiates RNA synthesis and copy vRNA into
CC complementary RNA (cRNA) which in turn serves as a template for the
CC production of more vRNAs. {ECO:0000250|UniProtKB:P03433}.
CC -!- SUBUNIT: RNA polymerase is composed of three subunits: PA, PB1 and PB2.
CC {ECO:0000250|UniProtKB:P03431}.
CC -!- SUBCELLULAR LOCATION: Virion. Host nucleus {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the influenza viruses PA family. {ECO:0000305}.
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DR EMBL; D00540; BAA00429.1; -; Genomic_RNA.
DR EMBL; AF006073; AAB62893.1; -; mRNA.
DR PIR; A33303; P2IVTV.
DR RefSeq; YP_145795.1; NC_006496.1.
DR PDB; 4CGX; X-ray; 2.70 A; A/B=1-170.
DR PDB; 4CHC; X-ray; 2.77 A; A/B/C/D/E/F=1-170.
DR PDBsum; 4CGX; -.
DR PDBsum; 4CHC; -.
DR SMR; P27194; -.
DR GeneID; 5075734; -.
DR KEGG; vg:5075734; -.
DR Proteomes; UP000008973; Genome.
DR GO; GO:0042025; C:host cell nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR GO; GO:0039694; P:viral RNA genome replication; IEA:InterPro.
DR Gene3D; 3.40.91.90; -; 1.
DR InterPro; IPR001009; PA/PA-X.
DR InterPro; IPR038372; PA/PA-X_sf.
DR Pfam; PF00603; Flu_PA; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Host nucleus; Reference proteome; Virion.
FT CHAIN 1..622
FT /note="Polymerase acidic protein"
FT /id="PRO_0000078811"
FT CONFLICT 190
FT /note="D -> N (in Ref. 1; BAA00429)"
FT /evidence="ECO:0000305"
FT CONFLICT 226..236
FT /note="SPWRALLLGAD -> AHGGLYCWE (in Ref. 1; BAA00429)"
FT /evidence="ECO:0000305"
FT CONFLICT 241
FT /note="V -> E (in Ref. 1; BAA00429)"
FT /evidence="ECO:0000305"
FT CONFLICT 245..299
FT /note="GTDQEAISWIHSQSEIECIRESKSTPASVITCLTSSLQSFAEGNPVRSRIHE
FT DII -> EQTKRPSLGYTLSLRLNALGNQIYPSISHHLPNVKPSIICRGEPVDL (in
FT Ref. 1; BAA00429)"
FT /evidence="ECO:0000305"
FT CONFLICT 310
FT /note="Missing (in Ref. 1; BAA00429)"
FT /evidence="ECO:0000305"
FT CONFLICT 377
FT /note="E -> V (in Ref. 1; BAA00429)"
FT /evidence="ECO:0000305"
FT CONFLICT 394
FT /note="A -> V (in Ref. 1; BAA00429)"
FT /evidence="ECO:0000305"
FT CONFLICT 405..408
FT /note="DRSR -> EIVAE (in Ref. 1; BAA00429)"
FT /evidence="ECO:0000305"
FT CONFLICT 469..471
FT /note="VEG -> EE (in Ref. 1; BAA00429)"
FT /evidence="ECO:0000305"
FT CONFLICT 493
FT /note="N -> G (in Ref. 1; BAA00429)"
FT /evidence="ECO:0000305"
FT CONFLICT 543
FT /note="W -> R (in Ref. 1; BAA00429)"
FT /evidence="ECO:0000305"
FT CONFLICT 576
FT /note="R -> E (in Ref. 1; BAA00429)"
FT /evidence="ECO:0000305"
FT CONFLICT 598..622
FT /note="NECIINNPIVAYLAKGWNSMPNVYY -> SLFGKRLEQHA (in Ref. 1;
FT BAA00429)"
FT /evidence="ECO:0000305"
FT STRAND 6..8
FT /evidence="ECO:0007829|PDB:4CGX"
FT HELIX 10..16
FT /evidence="ECO:0007829|PDB:4CGX"
FT HELIX 17..19
FT /evidence="ECO:0007829|PDB:4CGX"
FT TURN 22..24
FT /evidence="ECO:0007829|PDB:4CHC"
FT HELIX 28..45
FT /evidence="ECO:0007829|PDB:4CGX"
FT STRAND 55..59
FT /evidence="ECO:0007829|PDB:4CGX"
FT HELIX 65..74
FT /evidence="ECO:0007829|PDB:4CGX"
FT STRAND 81..83
FT /evidence="ECO:0007829|PDB:4CGX"
FT STRAND 86..89
FT /evidence="ECO:0007829|PDB:4CGX"
FT TURN 90..93
FT /evidence="ECO:0007829|PDB:4CGX"
FT STRAND 94..100
FT /evidence="ECO:0007829|PDB:4CGX"
FT STRAND 104..106
FT /evidence="ECO:0007829|PDB:4CGX"
FT STRAND 112..119
FT /evidence="ECO:0007829|PDB:4CGX"
FT HELIX 127..132
FT /evidence="ECO:0007829|PDB:4CGX"
FT HELIX 135..154
FT /evidence="ECO:0007829|PDB:4CGX"
FT HELIX 158..163
FT /evidence="ECO:0007829|PDB:4CGX"
SQ SEQUENCE 622 AA; 71465 MW; C5C1C85A6E4F7903 CRC64;
MTDRPDHIDS RVWELSETQE DWITQVHGHV RRVVECWKYT ICCLISNMHT HRGAPQYDVF
KWQDRSTIEW ICSKKKVQYP ERDTPDLYDN ERAVAYKVLL VSDLSDHSPT SGIYHDLAFN
LEGEAEESCA LVLRGSQLQD IKGFLCRALE WVVSNNLTQE VVETISGEAK LQFSVGTTFR
TLLKRDTDWD VIPTPRVEPN VPRIEGRRWT QMKKLPLLKE KEGPPSPWRA LLLGADSEYI
VCPPGTDQEA ISWIHSQSEI ECIRESKSTP ASVITCLTSS LQSFAEGNPV RSRIHEDIIA
FGINKKQEKK QSASSSASGE WKRAEYQVEE MSLPPWVEEE MVLLRSDQED NWIELEKNAI
YTEVDGVAEG LVDKYIEIVG RTKVASVIEK WQIAATRTFS QLHTDRSRIT ACPIITRDPS
GNCQFWGMVL LGPHHVKRDT DNAPLLIAEI MGEDTEEKYP KHSVFSLKVE GKQFLLSLKI
TSFSRNKLYT FSNIRRVLIQ PASIYSQVVL SRAAENNSLN LEVNPEIQLY LEGAQRGMTL
YQWVRMILCL EFLMAIYNNP QMEGFLANMR RLHMSRHAMM ERRQVFLPFG SRPEDKVNEC
IINNPIVAYL AKGWNSMPNV YY
