ID   PB1F2_I33A0             Reviewed;          90 AA.
AC   Q20MH0; Q1I2B0;
DT   20-FEB-2007, integrated into UniProtKB/Swiss-Prot.
DT   18-APR-2006, sequence version 1.
DT   23-FEB-2022, entry version 64.
DE   RecName: Full=Protein PB1-F2 {ECO:0000255|HAMAP-Rule:MF_04064};
GN   Name=PB1 {ECO:0000255|HAMAP-Rule:MF_04064};
OS   Influenza A virus (strain A/Wilson-Smith/1933 H1N1) (Influenza A virus
OS   (strain A/WS/1933 H1N1)).
OC   Viruses; Riboviria; Orthornavirae; Negarnaviricota; Polyploviricotina;
OC   Insthoviricetes; Articulavirales; Orthomyxoviridae; Alphainfluenzavirus.
OX   NCBI_TaxID=381518;
OH   NCBI_TaxID=8782; Aves.
OH   NCBI_TaxID=9606; Homo sapiens (Human).
OH   NCBI_TaxID=9823; Sus scrofa (Pig).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RC   STRAIN=A/WSN/1933, and A/WSN/1933 TS61;
RA   Ghedin E., Spiro D., Miller N., Zaborsky J., Feldblyum T., Subbu V.,
RA   Shumway M., Sparenborg J., Groveman L., Halpin R., Sitz J., Koo H.,
RA   Salzberg S.L., Webster R.G., Hoffmann E., Krauss S., Naeve C., Bao Y.,
RA   Bolotov P., Dernovoy D., Kiryutin B., Lipman D.J., Tatusova T.;
RT   "The NIAID influenza genome sequencing project.";
RL   Submitted (MAR-2006) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Plays an important role in promoting lung pathology in both
CC       primary viral infection and secondary bacterial infection. Promotes
CC       alteration of mitochondrial morphology, dissipation of mitochondrial
CC       membrane potential, and cell death. Alternatively, inhibits the
CC       production of interferon in the infected cell at the level of host
CC       mitochondrial antiviral signaling MAVS. Its level of expression differs
CC       greatly depending on which cell type is infected, in a manner that is
CC       independent of the levels of expression of other viral proteins.
CC       Monocytic cells are more affected than epithelial cells. Seems to
CC       disable virus-infected monocytes or other host innate immune cells.
CC       During early stage of infection, predisposes the mitochondria to
CC       permeability transition through interaction with host SLC25A6/ANT3 and
CC       VDAC1. These proteins participate in the formation of the permeability
CC       transition pore complex (PTPC) responsible of the release of
CC       mitochondrial products that triggers apoptosis. {ECO:0000255|HAMAP-
CC       Rule:MF_04064}.
CC   -!- SUBUNIT: Oligomer. Interacts with human SLC25A6/ANT3 and VDAC1.
CC       Interacts with host MAVS. {ECO:0000255|HAMAP-Rule:MF_04064}.
CC   -!- SUBCELLULAR LOCATION: Host mitochondrion inner membrane
CC       {ECO:0000255|HAMAP-Rule:MF_04064}. Host nucleus {ECO:0000255|HAMAP-
CC       Rule:MF_04064}. Host cytoplasm, host cytosol {ECO:0000255|HAMAP-
CC       Rule:MF_04064}. Note=Inner mitochondrial membrane in most cells types.
CC       Otherwise is detected in the nucleus and cytosol. {ECO:0000255|HAMAP-
CC       Rule:MF_04064}.
CC   -!- MISCELLANEOUS: Is not encoded in all strains, and seems to be
CC       dispensable for replication.
CC   -!- SIMILARITY: Belongs to the influenza viruses PB1-F2 family.
CC       {ECO:0000255|HAMAP-Rule:MF_04064}.
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DR   EMBL; CY009610; ABD77805.1; -; Genomic_RNA.
DR   EMBL; CY010794; ABF47964.1; -; Genomic_RNA.
DR   BMRB; Q20MH0; -.
DR   SMR; Q20MH0; -.
DR   Proteomes; UP000131702; Genome.
DR   Proteomes; UP000148454; Genome.
DR   GO; GO:0044164; C:host cell cytosol; IEA:UniProtKB-SubCell.
DR   GO; GO:0044192; C:host cell mitochondrial inner membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0042025; C:host cell nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0016020; C:membrane; IEA:UniProtKB-UniRule.
DR   GO; GO:0039526; P:modulation by virus of host apoptotic process; IEA:UniProtKB-KW.
DR   GO; GO:0039545; P:suppression by virus of host viral-induced cytoplasmic pattern recognition receptor signaling pathway via inhibition of MAVS activity; ISS:UniProtKB.
DR   HAMAP; MF_04064; INFV_PB1F2; 1.
DR   InterPro; IPR021045; Flu_proapoptotic_PB1-F2.
DR   Pfam; PF11986; PB1-F2; 1.
PE   3: Inferred from homology;
KW   Apoptosis; Host cytoplasm; Host membrane; Host mitochondrion;
KW   Host mitochondrion inner membrane; Host nucleus; Host-virus interaction;
KW   Inhibition of host innate immune response by virus;
KW   Inhibition of host MAVS by virus; Inhibition of host RLR pathway by virus;
KW   Membrane; Modulation of host cell apoptosis by virus; Viral immunoevasion.
FT   CHAIN           1..90
FT                   /note="Protein PB1-F2"
FT                   /id="PRO_0000278728"
FT   REGION          1..32
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          65..87
FT                   /note="Mitochondrial targeting sequence"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_04064"
FT   COMPBIAS        1..24
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   SITE            66
FT                   /note="Important for pathogenicity, S is highly pathogenic
FT                   whereas N is low pathogenic"
FT   SITE            66
FT                   /note="Low pathogenicity"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_04064"
FT   VARIANT         73..74
FT                   /note="KT -> RP (in strain: A/WSN/1933 TS61)"
SQ   SEQUENCE   90 AA;  10683 MW;  B70703482BCE448E CRC64;
     MGQEQDTPWI LSTGHISTQK GEDGQQTPKL EHRNSTRLMG HCQKTMNQVV MPKQIVYWKQ
     WPSLRNPILV SLKTRVLKRW RLFSKHEWTS