PB1F2_I97A1
ID PB1F2_I97A1 Reviewed; 90 AA.
AC P0C0U0;
DT 06-DEC-2005, integrated into UniProtKB/Swiss-Prot.
DT 06-DEC-2005, sequence version 1.
DT 23-FEB-2022, entry version 66.
DE RecName: Full=Protein PB1-F2 {ECO:0000255|HAMAP-Rule:MF_04064};
GN Name=PB1 {ECO:0000255|HAMAP-Rule:MF_04064};
OS Influenza A virus (strain A/Hong Kong/156/1997 H5N1 genotype Gs/Gd).
OC Viruses; Riboviria; Orthornavirae; Negarnaviricota; Polyploviricotina;
OC Insthoviricetes; Articulavirales; Orthomyxoviridae; Alphainfluenzavirus.
OX NCBI_TaxID=130763;
OH NCBI_TaxID=8782; Aves.
OH NCBI_TaxID=9685; Felis catus (Cat) (Felis silvestris catus).
OH NCBI_TaxID=9606; Homo sapiens (Human).
OH NCBI_TaxID=9691; Panthera pardus (Leopard) (Felis pardus).
OH NCBI_TaxID=9694; Panthera tigris (Tiger).
OH NCBI_TaxID=9823; Sus scrofa (Pig).
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX PubMed=9430591; DOI=10.1126/science.279.5349.393;
RA Subbarao K., Klimov A., Katz J., Regnery H., Lim W., Hall H., Perdue M.,
RA Swayne D., Bender C., Huang J., Hemphill M., Rowe T., Shaw M., Xu X.,
RA Fukuda K., Cox N.;
RT "Characterization of an avian influenza A (H5N1) virus isolated from a
RT child with a fatal respiratory illness.";
RL Science 279:393-396(1998).
RN [2]
RP MUTAGENESIS OF ASN-66.
RX PubMed=17922571; DOI=10.1371/journal.ppat.0030141;
RA Conenello G.M., Zamarin D., Perrone L.A., Tumpey T., Palese P.;
RT "A single mutation in the PB1-F2 of H5N1 (HK/97) and 1918 Influenza A
RT viruses contributes to increased virulence.";
RL PLoS Pathog. 3:1414-1421(2007).
CC -!- FUNCTION: Plays an important role in promoting lung pathology in both
CC primary viral infection and secondary bacterial infection. Promotes
CC alteration of mitochondrial morphology, dissipation of mitochondrial
CC membrane potential, and cell death. Alternatively, inhibits the
CC production of interferon in the infected cell at the level of host
CC mitochondrial antiviral signaling MAVS. Its level of expression differs
CC greatly depending on which cell type is infected, in a manner that is
CC independent of the levels of expression of other viral proteins.
CC Monocytic cells are more affected than epithelial cells. Seems to
CC disable virus-infected monocytes or other host innate immune cells.
CC During early stage of infection, predisposes the mitochondria to
CC permeability transition through interaction with host SLC25A6/ANT3 and
CC VDAC1. These proteins participate in the formation of the permeability
CC transition pore complex (PTPC) responsible of the release of
CC mitochondrial products that triggers apoptosis. {ECO:0000255|HAMAP-
CC Rule:MF_04064}.
CC -!- SUBUNIT: Oligomer. Interacts with human SLC25A6/ANT3 and VDAC1.
CC Interacts with host MAVS. {ECO:0000255|HAMAP-Rule:MF_04064}.
CC -!- SUBCELLULAR LOCATION: Host mitochondrion inner membrane
CC {ECO:0000255|HAMAP-Rule:MF_04064}. Host nucleus {ECO:0000255|HAMAP-
CC Rule:MF_04064}. Host cytoplasm, host cytosol {ECO:0000255|HAMAP-
CC Rule:MF_04064}. Note=Inner mitochondrial membrane in most cells types.
CC Otherwise is detected in the nucleus and cytosol. {ECO:0000255|HAMAP-
CC Rule:MF_04064}.
CC -!- MISCELLANEOUS: Is not encoded in all strains, and seems to be
CC dispensable for replication.
CC -!- SIMILARITY: Belongs to the influenza viruses PB1-F2 family.
CC {ECO:0000255|HAMAP-Rule:MF_04064}.
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DR EMBL; AF036362; -; NOT_ANNOTATED_CDS; Genomic_RNA.
DR SMR; P0C0U0; -.
DR Proteomes; UP000008587; Genome.
DR GO; GO:0044164; C:host cell cytosol; IEA:UniProtKB-SubCell.
DR GO; GO:0044192; C:host cell mitochondrial inner membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0042025; C:host cell nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0016020; C:membrane; IEA:UniProtKB-UniRule.
DR GO; GO:0039526; P:modulation by virus of host apoptotic process; IEA:UniProtKB-KW.
DR GO; GO:0039545; P:suppression by virus of host viral-induced cytoplasmic pattern recognition receptor signaling pathway via inhibition of MAVS activity; IEA:UniProtKB-KW.
DR HAMAP; MF_04064; INFV_PB1F2; 1.
DR InterPro; IPR021045; Flu_proapoptotic_PB1-F2.
DR Pfam; PF11986; PB1-F2; 1.
PE 1: Evidence at protein level;
KW Apoptosis; Host cytoplasm; Host membrane; Host mitochondrion;
KW Host mitochondrion inner membrane; Host nucleus; Host-virus interaction;
KW Inhibition of host innate immune response by virus;
KW Inhibition of host MAVS by virus; Inhibition of host RLR pathway by virus;
KW Membrane; Modulation of host cell apoptosis by virus; Viral immunoevasion.
FT CHAIN 1..90
FT /note="Protein PB1-F2"
FT /id="PRO_0000078742"
FT REGION 1..34
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 65..87
FT /note="Mitochondrial targeting sequence"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_04064"
FT SITE 66
FT /note="Low pathogenicity"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_04064"
FT MUTAGEN 66
FT /note="N->S: Increased pathogenicity, and 100-fold increase
FT of virus replication in mice."
FT /evidence="ECO:0000269|PubMed:17922571"
SQ SEQUENCE 90 AA; 10757 MW; 0E39C2AAB36F726D CRC64;
MEQEQDTPWT QSTEHINIQK KGGGQQTQRP EHPNSTLLMD HYLKITSRAG MHKQIVYWKQ
WLSLKNPTQD SLKTRVLKRW KLSSKREWIS
