ID   ASPA_AERSA              Reviewed;         621 AA.
AC   P31339;
DT   01-JUL-1993, integrated into UniProtKB/Swiss-Prot.
DT   01-JUL-1993, sequence version 1.
DT   03-AUG-2022, entry version 90.
DE   RecName: Full=Microbial serine proteinase;
DE            EC=3.4.21.-;
DE   Flags: Precursor;
GN   Name=aspA;
OS   Aeromonas salmonicida.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Aeromonadales;
OC   Aeromonadaceae; Aeromonas.
OX   NCBI_TaxID=645;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PROTEIN SEQUENCE OF 25-30; 322-339
RP   AND 368-386.
RC   STRAIN=MT0004 / U2862;
RX   PubMed=1468618; DOI=10.1016/0378-1097(92)90289-z;
RA   Whitby P.W., Landon M., Coleman G.;
RT   "The cloning and nucleotide sequence of the serine protease gene (aspA) of
RT   Aeromonas salmonicida ssp. salmonicida.";
RL   FEMS Microbiol. Lett. 78:65-71(1992).
CC   -!- FUNCTION: Agent of furonculosis.
CC   -!- SIMILARITY: Belongs to the peptidase S8 family. {ECO:0000305}.
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DR   EMBL; X67043; CAA47428.1; ALT_SEQ; Genomic_DNA.
DR   PIR; S26691; S26691.
DR   AlphaFoldDB; P31339; -.
DR   SMR; P31339; -.
DR   GO; GO:0004252; F:serine-type endopeptidase activity; IEA:InterPro.
DR   GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR   Gene3D; 3.40.50.200; -; 1.
DR   InterPro; IPR008979; Galactose-bd-like_sf.
DR   InterPro; IPR002884; P_dom.
DR   InterPro; IPR000209; Peptidase_S8/S53_dom.
DR   InterPro; IPR036852; Peptidase_S8/S53_dom_sf.
DR   InterPro; IPR023827; Peptidase_S8_Asp-AS.
DR   InterPro; IPR023828; Peptidase_S8_Ser-AS.
DR   InterPro; IPR015500; Peptidase_S8_subtilisin-rel.
DR   Pfam; PF01483; P_proprotein; 1.
DR   Pfam; PF00082; Peptidase_S8; 1.
DR   PRINTS; PR00723; SUBTILISIN.
DR   SUPFAM; SSF49785; SSF49785; 1.
DR   SUPFAM; SSF52743; SSF52743; 1.
DR   PROSITE; PS51829; P_HOMO_B; 1.
DR   PROSITE; PS51892; SUBTILASE; 1.
DR   PROSITE; PS00136; SUBTILASE_ASP; 1.
DR   PROSITE; PS00138; SUBTILASE_SER; 1.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Hydrolase; Protease; Serine protease; Signal.
FT   SIGNAL          1..24
FT                   /evidence="ECO:0000255"
FT   CHAIN           25..621
FT                   /note="Microbial serine proteinase"
FT                   /id="PRO_0000026995"
FT   DOMAIN          68..440
FT                   /note="Peptidase S8"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01240"
FT   DOMAIN          454..619
FT                   /note="P/Homo B"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01173"
FT   REGION          114..133
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          457..485
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        469..485
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   ACT_SITE        98
FT                   /note="Charge relay system"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01240"
FT   ACT_SITE        137
FT                   /note="Charge relay system"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01240"
FT   ACT_SITE        354
FT                   /note="Charge relay system"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01240"
SQ   SEQUENCE   621 AA;  66620 MW;  4A2597290F2C8D7E CRC64;
     MRKTSLALAI SALLSALPIA SVQANESCTP LTGKEAGLDT GRSSAVRCLP GINPLQDLLN
     SGQNAFSPRG GMAGNDLNLW WAHRTEVLGQ GINVAVVDDG LAIAHPDLAD NVRPGSKNVV
     TGGSDPTPTD PDRCPRHSVS GIIAAVDNSI GTLGVAPRVQ LQGFNLLDDN IQQLQKDWLY
     ALGQRRHRRQ PGLQPELRMS LVDPEGANGL DQVQLDRLFE QRTQHASAAY IKAAGTAFSR
     IAAGNYVAQP HRNLPKLPFE NSNIDPSNSN FWNLVVRAIN ADGVRSSYSS VGSNVFLSAP
     GGEYGTDAPA MVTTDLPGCD MGYNRVDDPS TNRLHNNPQL DASCDYNGVM NGTSSATPNT
     TGAMVLMAPY PDLSVRDLRD LLARNATRLD ANQGPVQINY TAANGERRQV TGLEGWERNA
     AGLWYSPSYG FGLVDVNKTQ PCSRQPRTAA TTGAVALAKG KGNGRSPSAP SRYVGSSPTR
     SSTQVDQPLT VEAVQVMVSL DHQRLPDLLI ELVSPSGTRS VLLNPNNSLV GQSLDRQQLG
     YVRTKGLRDM RMLSHKFYGE PAHGEWRLEV TDVANAAAQV SLLDRRTNTR STLTEGNNSQ
     PGQLLDWSRG YSVLGHDAAR S