ID   PB2_I01A3               Reviewed;         742 AA.
AC   Q809P5;
DT   13-NOV-2007, integrated into UniProtKB/Swiss-Prot.
DT   22-JUL-2008, sequence version 2.
DT   23-FEB-2022, entry version 61.
DE   RecName: Full=Polymerase basic protein 2;
DE   AltName: Full=RNA-directed RNA polymerase subunit P3;
DE   Flags: Fragment;
GN   Name=PB2;
OS   Influenza A virus (strain A/Chicken/Hong Kong/715.5/2001 H5N1 genotype E).
OC   Viruses; Riboviria; Orthornavirae; Negarnaviricota; Polyploviricotina;
OC   Insthoviricetes; Articulavirales; Orthomyxoviridae; Alphainfluenzavirus.
OX   NCBI_TaxID=196434;
OH   NCBI_TaxID=8782; Aves.
OH   NCBI_TaxID=9685; Felis catus (Cat) (Felis silvestris catus).
OH   NCBI_TaxID=9606; Homo sapiens (Human).
OH   NCBI_TaxID=9691; Panthera pardus (Leopard) (Felis pardus).
OH   NCBI_TaxID=9694; Panthera tigris (Tiger).
OH   NCBI_TaxID=9823; Sus scrofa (Pig).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX   PubMed=12077307; DOI=10.1073/pnas.132268999;
RA   Guan Y., Peiris J.S.M., Lipatov A.S., Ellis T.M., Dyrting K.C., Krauss S.,
RA   Zhang L.J., Webster R.G., Shortridge K.F.;
RT   "Emergence of multiple genotypes of H5N1 avian influenza viruses in Hong
RT   Kong SAR.";
RL   Proc. Natl. Acad. Sci. U.S.A. 99:8950-8955(2002).
RN   [2]
RP   SEQUENCE REVISION.
RA   Li K.S., Xu K.M., Guan Y.;
RL   Submitted (MAR-2008) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Plays an essential role in transcription initiation and cap-
CC       stealing mechanism, in which cellular capped pre-mRNAs are used to
CC       generate primers for viral transcription. Binds the cap of the target
CC       pre-RNA which is subsequently cleaved after 10-13 nucleotides by PA.
CC       Plays a role in the initiation of the viral genome replication and
CC       modulates the activity of the ribonucleoprotein (RNP) complex. In
CC       addition, participates in the inhibition of type I interferon induction
CC       through interaction with the host mitochondrial antiviral signaling
CC       protein MAVS. {ECO:0000250|UniProtKB:P03428}.
CC   -!- SUBUNIT: Influenza RNA polymerase is composed of three subunits: PB1,
CC       PB2 and PA. Interacts (via N-terminus) with PB1 (via C-terminus).
CC       Interacts with nucleoprotein NP (via N-terminus). Interacts (via N-
CC       terminus) with host MAVS (via N-terminus); this interaction inhibits
CC       host innate immune response. {ECO:0000250|UniProtKB:P03428}.
CC   -!- SUBCELLULAR LOCATION: Virion. Host nucleus
CC       {ECO:0000250|UniProtKB:P03428}. Host mitochondrion
CC       {ECO:0000250|UniProtKB:P03428}.
CC   -!- SIMILARITY: Belongs to the influenza viruses PB2 family. {ECO:0000305}.
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DR   EMBL; AF509152; AAO52995.2; -; Genomic_DNA.
DR   SMR; Q809P5; -.
DR   GO; GO:0033650; C:host cell mitochondrion; IEA:UniProtKB-SubCell.
DR   GO; GO:0042025; C:host cell nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR   GO; GO:0006370; P:7-methylguanosine mRNA capping; IEA:UniProtKB-KW.
DR   GO; GO:0075526; P:cap snatching; IEA:UniProtKB-KW.
DR   GO; GO:0039523; P:suppression by virus of host mRNA transcription via inhibition of RNA polymerase II activity; IEA:UniProtKB-KW.
DR   GO; GO:0039545; P:suppression by virus of host viral-induced cytoplasmic pattern recognition receptor signaling pathway via inhibition of MAVS activity; IEA:UniProtKB-KW.
DR   GO; GO:0006351; P:transcription, DNA-templated; IEA:InterPro.
DR   GO; GO:0039694; P:viral RNA genome replication; IEA:InterPro.
DR   Gene3D; 3.30.30.90; -; 1.
DR   InterPro; IPR001591; INV_PB2.
DR   InterPro; IPR037258; PDB2_C.
DR   Pfam; PF00604; Flu_PB2; 1.
DR   SUPFAM; SSF160453; SSF160453; 1.
PE   3: Inferred from homology;
KW   Cap snatching; Eukaryotic host gene expression shutoff by virus;
KW   Eukaryotic host transcription shutoff by virus;
KW   Host gene expression shutoff by virus; Host mitochondrion; Host nucleus;
KW   Host-virus interaction; Inhibition of host innate immune response by virus;
KW   Inhibition of host MAVS by virus; Inhibition of host RLR pathway by virus;
KW   Inhibition of host RNA polymerase II by virus; mRNA capping;
KW   mRNA processing; Viral immunoevasion; Viral transcription; Virion.
FT   CHAIN           1..>742
FT                   /note="Polymerase basic protein 2"
FT                   /id="PRO_0000311147"
FT   MOTIF           736..739
FT                   /note="Nuclear localization signal"
FT                   /evidence="ECO:0000250"
FT   NON_TER         742
SQ   SEQUENCE   742 AA;  83918 MW;  CEDF51FD966B63B7 CRC64;
     MERIKELRDL MSQSRTREIL TKTTVDHMAI IKKYTSGRQE KNPALRMKWM MAMKYPITAD
     KRIIEMIPER NEQGQTLWSK TNDAGSDRVM VSPLAVTWWN RNGPTTSAVH YPKVYKTYFE
     KVERLKHGTF GPVHFRNQVK IRRRVDINPG HADLSAKEAQ DVIMEVVFPN EVGARILTSE
     SQLTITKEKK EELQDCKIAP LMVAYMLERE LVRKTRFLPV AGGTSSVYIE VLHLTQGTCW
     EQMYTPGGEV RNDDVDQSLI IAARNIVRRA TVSADPLASL LEMCHSTQIG GIRMVDILRQ
     NPTEEQAVDI CKAAMGLRIS SSFSFGGFTF KRTSGSSVKK EEEVLTGNLQ TLKIRVHEGY
     EEFTMVGRRA TAILRKATRR LIQLIVSGRD EQSIAEAIIV AMVFSQEDCM IKAVRGDLNF
     VNRANQRLNP MHQLLRHFQK DAKVLFQNWG IEPIDNVMGM IGILPDMTPS TEMSLRGVRV
     SKMGVDEYSS TERVVVSIDR FLRVRDQRGN VLLSPEEVSE TQGTEKLTIT YSSSMMWEIN
     GPESVLVNTY QWIIRNWETV KIQWSQDPTM LYNKMEFEPF QSLVPKAARG QYSGFVRTLF
     QQMRDVLGTF DTVQIIKLLP FAAAPPEQSR MQFSSLTVNV RGSGMRILVR GNSPVFNYNK
     ATKRLTVLGK DAGALTEDPD EGTAGVESAV LRGFLILGKE DKRYGPALSI NELSNLAKGE
     KANVLIGQGD VVLVMKRKRD SS