ID   PB2_I02A1               Reviewed;         736 AA.
AC   Q6DNM2;
DT   13-NOV-2007, integrated into UniProtKB/Swiss-Prot.
DT   16-AUG-2004, sequence version 1.
DT   23-FEB-2022, entry version 64.
DE   RecName: Full=Polymerase basic protein 2;
DE   AltName: Full=RNA-directed RNA polymerase subunit P3;
DE   Flags: Fragment;
GN   Name=PB2;
OS   Influenza A virus (strain A/Guinea fowl/Hong Kong/38/2002 H5N1 genotype
OS   X0).
OC   Viruses; Riboviria; Orthornavirae; Negarnaviricota; Polyploviricotina;
OC   Insthoviricetes; Articulavirales; Orthomyxoviridae; Alphainfluenzavirus.
OX   NCBI_TaxID=284208;
OH   NCBI_TaxID=8782; Aves.
OH   NCBI_TaxID=9685; Felis catus (Cat) (Felis silvestris catus).
OH   NCBI_TaxID=9606; Homo sapiens (Human).
OH   NCBI_TaxID=9691; Panthera pardus (Leopard) (Felis pardus).
OH   NCBI_TaxID=9694; Panthera tigris (Tiger).
OH   NCBI_TaxID=9823; Sus scrofa (Pig).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX   PubMed=15241415; DOI=10.1038/nature02746;
RA   Li K.S., Guan Y., Wang J., Smith G.J.D., Xu K.M., Duan L., Rahardjo A.P.,
RA   Puthavathana P., Buranathai C., Nguyen T.D., Estoepangestie A.T.S.,
RA   Chaisingh A., Auewarakul P., Long H.T., Hanh N.T.H., Webby R.J.,
RA   Poon L.L.M., Chen H., Shortridge K.F., Yuen K.Y., Webster R.G.,
RA   Peiris J.S.M.;
RT   "Genesis of a highly pathogenic and potentially pandemic H5N1 influenza
RT   virus in eastern Asia.";
RL   Nature 430:209-213(2004).
CC   -!- FUNCTION: Plays an essential role in transcription initiation and cap-
CC       stealing mechanism, in which cellular capped pre-mRNAs are used to
CC       generate primers for viral transcription. Binds the cap of the target
CC       pre-RNA which is subsequently cleaved after 10-13 nucleotides by PA.
CC       Plays a role in the initiation of the viral genome replication and
CC       modulates the activity of the ribonucleoprotein (RNP) complex. In
CC       addition, participates in the inhibition of type I interferon induction
CC       through interaction with the host mitochondrial antiviral signaling
CC       protein MAVS. {ECO:0000250|UniProtKB:P03428}.
CC   -!- SUBUNIT: Influenza RNA polymerase is composed of three subunits: PB1,
CC       PB2 and PA. Interacts (via N-terminus) with PB1 (via C-terminus).
CC       Interacts with nucleoprotein NP (via N-terminus). Interacts (via N-
CC       terminus) with host MAVS (via N-terminus); this interaction inhibits
CC       host innate immune response. {ECO:0000250|UniProtKB:P03428}.
CC   -!- SUBCELLULAR LOCATION: Virion. Host nucleus
CC       {ECO:0000250|UniProtKB:P03428}. Host mitochondrion
CC       {ECO:0000250|UniProtKB:P03428}.
CC   -!- SIMILARITY: Belongs to the influenza viruses PB2 family. {ECO:0000305}.
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DR   EMBL; AY651730; AAT73561.1; -; Genomic_RNA.
DR   SMR; Q6DNM2; -.
DR   GO; GO:0033650; C:host cell mitochondrion; IEA:UniProtKB-SubCell.
DR   GO; GO:0042025; C:host cell nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR   GO; GO:0006370; P:7-methylguanosine mRNA capping; IEA:UniProtKB-KW.
DR   GO; GO:0075526; P:cap snatching; IEA:UniProtKB-KW.
DR   GO; GO:0039523; P:suppression by virus of host mRNA transcription via inhibition of RNA polymerase II activity; IEA:UniProtKB-KW.
DR   GO; GO:0039545; P:suppression by virus of host viral-induced cytoplasmic pattern recognition receptor signaling pathway via inhibition of MAVS activity; IEA:UniProtKB-KW.
DR   GO; GO:0006351; P:transcription, DNA-templated; IEA:InterPro.
DR   GO; GO:0039694; P:viral RNA genome replication; IEA:InterPro.
DR   Gene3D; 3.30.30.90; -; 1.
DR   InterPro; IPR001591; INV_PB2.
DR   InterPro; IPR037258; PDB2_C.
DR   Pfam; PF00604; Flu_PB2; 1.
DR   SUPFAM; SSF160453; SSF160453; 1.
PE   3: Inferred from homology;
KW   Cap snatching; Eukaryotic host gene expression shutoff by virus;
KW   Eukaryotic host transcription shutoff by virus;
KW   Host gene expression shutoff by virus; Host mitochondrion; Host nucleus;
KW   Host-virus interaction; Inhibition of host innate immune response by virus;
KW   Inhibition of host MAVS by virus; Inhibition of host RLR pathway by virus;
KW   Inhibition of host RNA polymerase II by virus; mRNA capping;
KW   mRNA processing; Viral immunoevasion; Viral transcription; Virion.
FT   CHAIN           <1..>736
FT                   /note="Polymerase basic protein 2"
FT                   /id="PRO_0000311148"
FT   MOTIF           728..731
FT                   /note="Nuclear localization signal"
FT                   /evidence="ECO:0000250"
FT   NON_TER         1
FT   NON_TER         736
SQ   SEQUENCE   736 AA;  83280 MW;  1005854658372B05 CRC64;
     NLMSQPRTRE ILTKTTVDHM AIIKKYTSGR QEKNPALRMK WMMAMKYPIT ADKRIMEMIP
     ERNEQGQTLW SKTNDAGSDR VMVSSLAVTW WNRNGPTTST IHYPKVYKTY FEKVERLKHG
     TFGPVHFRNQ VKIRRRVDVN PGHADLSAKE AQDVIMEVVF PNEVGARILT SESQLDNNKR
     EEEELQDCKI APLMVAYMLE RELVRKTRFL PVAGGTSSVY IEVLHLTQGT CWEQMYTPGG
     EVRNDDVDQS LIIAARNIVR RATVSADPLA SLLEMCHSTQ IGGIRMVDIL RQNPTEEQAV
     DICKAAMGLR ISSSFSFGGF TFKRTSGSSV KKEEEVLTGN LQTLKIRVHE GYEEFTMVGR
     RATAILRKAT RRLIQLIVSG RDEQSIAEAI IVAMVFSQED CMIKAVRGDL NFVNRANQRL
     NPMHQLLRHF QKDAKVLFQN WGIELIDNVM GMIGILPDMT PSTEMSLRGV RVSKMGVDEY
     SSTERVVVSI DRFLRVRDQR GNVLLSPEEV SETQGTEKLT ITYSSSMMWE INGPESVLVN
     TYQWIIRNWE TVKIQWSQDP TMLYNKMEFE PFQSLIPKAV RGQYSGFVRT LFQQMRDVLG
     TFDTVQIIKL LPFTAAPPEQ SRMQFSSLTV NVRGSGMRIL VRGNSPVFNY NKATKRLTVL
     GKDAGALTED PDEGTAGVES AVLRGFLILG KEDKRYGPAL SINELSNLAK GEKANVLIGQ
     GDVVLVMKRK RDSSIL